IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tillman-2007-1 DJ-1 binds androgen receptor directly and mediates its activity in hormonally treated prostate cancer cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tillman JE., Yuan J., Gu G., Fazli L., Ghosh R., Flynt AS., Gleave M., Rennie PS., Kasper S. Parkinsons - Interactions investigated in the context of Parkinsons disease susan.kasper@vanderbilt.edu A cDNA library generated from flutamide-activated primary HPE cells was used. Cancer Res. (0008-5472) 2007 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer tillman-2007-2 DJ-1 binds androgen receptor directly and mediates its activity in hormonally treated prostate cancer cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Tillman JE., Yuan J., Gu G., Fazli L., Ghosh R., Flynt AS., Gleave M., Rennie PS., Kasper S. Parkinsons - Interactions investigated in the context of Parkinsons disease susan.kasper@vanderbilt.edu Cancer Res. (0008-5472) 2007 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer A cDNA library generated from flutamide-activated primary HPE cells was used. tillman-2007-3 DJ-1 binds androgen receptor directly and mediates its activity in hormonally treated prostate cancer cells. human-lapc4 Homo sapiens LAPC4 cells derived from a human prostate cancer xenograft lapc4 Human prostate cancer xenograft cell line LAPC4 LAPC4 cells were derived from a human prostate cancer xenograft implanted in SCID mice and express wild-type androgen receptor anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tillman JE., Yuan J., Gu G., Fazli L., Ghosh R., Flynt AS., Gleave M., Rennie PS., Kasper S. Parkinsons - Interactions investigated in the context of Parkinsons disease susan.kasper@vanderbilt.edu Cancer Res. (0008-5472) 2007 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer A cDNA library generated from flutamide-activated primary HPE cells was used. tillman-2007-4 DJ-1 binds androgen receptor directly and mediates its activity in hormonally treated prostate cancer cells. human-lapc4 Homo sapiens LAPC4 cells derived from a human prostate cancer xenograft lapc4 Human prostate cancer xenograft cell line LAPC4 LAPC4 cells were derived from a human prostate cancer xenograft implanted in SCID mice and express wild-type androgen receptor anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Tillman JE., Yuan J., Gu G., Fazli L., Ghosh R., Flynt AS., Gleave M., Rennie PS., Kasper S. Parkinsons - Interactions investigated in the context of Parkinsons disease susan.kasper@vanderbilt.edu Cancer Res. (0008-5472) 2007 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer A cDNA library generated from flutamide-activated primary HPE cells was used. tillman-2007-5 DJ-1 binds androgen receptor directly and mediates its activity in hormonally treated prostate cancer cells. human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tillman JE., Yuan J., Gu G., Fazli L., Ghosh R., Flynt AS., Gleave M., Rennie PS., Kasper S. Parkinsons - Interactions investigated in the context of Parkinsons disease susan.kasper@vanderbilt.edu Cancer Res. (0008-5472) 2007 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer A cDNA library generated from flutamide-activated primary HPE cells was used. tillman-2007-6 DJ-1 binds androgen receptor directly and mediates its activity in hormonally treated prostate cancer cells. human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma confocal microscopy confocal microscopy predetermined predetermined participant predetermined Tillman JE., Yuan J., Gu G., Fazli L., Ghosh R., Flynt AS., Gleave M., Rennie PS., Kasper S. Parkinsons - Interactions investigated in the context of Parkinsons disease susan.kasper@vanderbilt.edu Cancer Res. (0008-5472) 2007 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer A cDNA library generated from flutamide-activated primary HPE cells was used. park7_human Parkinson disease protein 7 PARK7 Oncogene DJ1 Parkinsonism-associated deglycase Maillard deglycase Protein DJ-1 Protein/nucleic acid deglycase DJ-1 protein protein human Homo sapiens Human MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVVICPDASLEDAKKEGPYDVVVLPGGNLGAQNLSESAAVKEILKEQENRKGLIAAICAGPTALLAHEIGFGSKVTTHPLAKDKMMNGGHYTYSENRVEKDGLILTSRGPGTSFEFALAIVEALNGKEVAAQVKAPLVLKD andr_human Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 AR DHTR NR3C4 protein protein human Homo sapiens Human MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSALECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLKDILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKSTEDTAEYSPFKGGYTKGLEGESLGCSGSAAAGSSGTLELPSTLSLYKSGALDEAAAYQSRDYYNFPLALAGPPPPPPPPHPHARIKLENPLDYGSAWAAAAAQCRYGDLASLHGAGAAGPGSGSPSAAASSSWHTLFTAEEGQLYGPCGGGGGGGGGGGGGGGGGGGGGGGEAGAVAPYGYTRPPQGLAGQESDFTAPDVWYPGGMVSRVPYPSPTCVKSEMGPWMDSYSGPYGDMRLETARDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ park7-ar DJ-1 interacts with Androgen Receptor (bp 1377-2406) in a yeast two-hybrid assay 662257 N/A 662263 unspecified role unspecified role bait bait N/A 662264 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false park7-ar-1 DJ-1 interacts with Androgen Receptor (bp 1377-2406) in a yeast two-hybrid assay 662258 N/A 662264 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 662263 unspecified role unspecified role bait bait physical association physical association false false false 1 B ar-park7 Androgen Receptor coimmunoprecipitates DJ-1 in LAPC4 lysates 662259 N/A 662263 unspecified role unspecified role prey prey N/A 662264 unspecified role unspecified role bait bait physical association physical association false false false 1 C Dihydrotestosterone (DHT) - Treatment with DHT increased the interaction between DJ-1 and AR. park7-ar-2 Androgen Receptor coimmunoprecipitates with DJ-1 in LAPC4 lysates 662260 N/A 662264 unspecified role unspecified role prey prey N/A 662263 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 D Q99497 transfection ar-park7-1 Androgen Receptor coimmunoprecipitates DJ-1 in LAPC4 lysates 662261 N/A 662264 cofactor cofactor coenzyme bait bait N/A 662263 cofactor cofactor coenzyme prey prey physical association physical association false false false 2 Dihydrotestosterone (DHT) - Treatment with DHT increased the interaction between DJ-1 and AR. ar-park7-2 Androgen Receptor and DJ-1 colocalize in LNCaP cells 662262 N/A 662263 unspecified role unspecified role neutral component neutral component N/A 662264 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3 DJ-1-AR colocalization was greatest with DHT treatment, followed by OH-flutamide and bicalutamide. DHT and bicalutamide treatment resulted in similar colocalization throughout the nucleus, whereas DJ-1-AR with OH-flutamide treatment seemed to concentrate just within the nuclear perimeter.