IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rubin-2007-5 A role for the HOXB7 homeodomain protein in DNA repair. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Cancer Res. (0008-5472) 2007 rubin-2007-1 A role for the HOXB7 homeodomain protein in DNA repair. in vitro In vitro pull down pull down protein sequence protein sequence identification protein sequence Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Cancer Res. (0008-5472) 2007 rubin-2007-3 A role for the HOXB7 homeodomain protein in DNA repair. human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Cancer Res. (0008-5472) 2007 rubin-2007-4 A role for the HOXB7 homeodomain protein in DNA repair. human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Cancer Res. (0008-5472) 2007 rubin-2007-2 A role for the HOXB7 homeodomain protein in DNA repair. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Cancer Res. (0008-5472) 2007 rubin-2007-6 A role for the HOXB7 homeodomain protein in DNA repair. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Cancer Res. (0008-5472) 2007 xrcc5_human X-ray repair cross-complementing protein 5 XRCC5 G22P2 ATP-dependent DNA helicase II 80 kDa subunit Lupus Ku autoantigen protein p86 86 kDa subunit of Ku antigen Ku86 Ku80 Thyroid-lupus autoantigen CTC box-binding factor 85 kDa subunit Nuclear factor IV DNA repair protein XRCC5 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) ATP-dependent DNA helicase 2 subunit 2 protein protein human Homo sapiens Human MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAKKVITMFVQRQVFAENKDEIALVLFGTDGTDNPLSGGDQYQNITVHRHLMLPDFDLLEDIESKIQPGSQQADFLDALIVSMDVIQHETIGKKFEKRHIEIFTDLSSRFSKSQLDIIIHSLKKCDISLQFFLPFSLGKEDGSGDRGDGPFRLGGHGPSFPLKGITEQQKEGLEIVKMVMISLEGEDGLDEIYSFSESLRKLCVFKKIERHSIHWPCRLTIGSNLSIRIAAYKSILQERVKKTWTVVDAKTLKKEDIQKETVYCLNDDDETEVLKEDIIQGFRYGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQVQRRFFMGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVAIVRYAYDKRANPQVGVAFPHIKHNYECLVYVQLPFMEDLRQYMFSSLKNSKKYAPTEAQLNAVDALIDSMSLAKKDEKTDTLEDLFPTTKIPNPRFQRLFQCLLHRALHPREPLPPIQQHIWNMLNPPAEVTTKSQIPLSKIKTLFPLIEAKKKDQVTAQEIFQDNHEDGPTAKKLKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEASNQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI xrcc6_human X-ray repair cross-complementing protein 6 XRCC6 G22P1 ATP-dependent DNA helicase II 70 kDa subunit Lupus Ku autoantigen protein p70 70 kDa subunit of Ku antigen Thyroid-lupus autoantigen CTC box-binding factor 75 kDa subunit DNA repair protein XRCC6 X-ray repair complementing defective repair in Chinese hamster cells 6 ATP-dependent DNA helicase 2 subunit 1 5'-deoxyribose-5-phosphate lyase Ku70 protein protein human Homo sapiens Human MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD hxb7_human Homeobox protein Hox-B7 HOXB7 HOX2C Homeobox protein Hox-2C Homeobox protein HHO.C1 protein protein human Homo sapiens Human MSSLYYANTLFSKYPASSSVFATGAFPEQTSCAFASNPQRPGYGAGSGASFAASMQGLYPGGGGMAGQSAAGVYAAGYGLEPSSFNMHCAPFEQNLSGVCPGDSAKAAGAKEQRDSDLAAESNFRIYPWMRSSGTDRKRGRQTYTRYQTLELEKEFHYNRYLTRRRRIEIAHTLCLTERQIKIWFQNRRMKWKKENKTAGPGTTGQDRAEAEEEEEE prkdc_human DNA-dependent protein kinase catalytic subunit PRKDC HYRC HYRC1 DNPK1 p460 protein protein human Homo sapiens Human MAGSGAGVRCSLLRLQETLSAADRCGAALAGHQLIRGLGQECVLSSSPAVLALQTSLVFSRDFGLLVFVRKSLNSIEFRECREEILKFLCIFLEKMGQKIAPYSVEIKNTCTSVYTKDRAAKCKIPALDLLIKLLQTFRSSRLMDEFKIGELFSKFYGELALKKKIPDTVLEKVYELLGLLGEVHPSEMINNAENLFRAFLGELKTQMTSAVREPKLPVLAGCLKGLSSLLCNFTKSMEEDPQTSREIFNFVLKAIRPQIDLKRYAVPSAGLRLFALHASQFSTCLLDNYVSLFEVLLKWCAHTNVELKKAALSALESFLKQVSNMVAKNAEMHKNKLQYFMEQFYGIIRNVDSNNKELSIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTQTDTGDDRVYQMPSFLQSVASVLLYLDTVPEVYTPVLEHLVVMQIDSFPQYSPKMQLVCCRAIVKVFLALAAKGPVLRNCISTVVHQGLIRICSKPVVLPKGPESESEDHRASGEVRTGKWKVPTYKDYVDLFRHLLSSDQMMDSILADEAFFSVNSSSESLNHLLYDEFVKSVLKIVEKLDLTLEIQTVGEQENGDEAPGVWMIPTSDPAANLHPAKPKDFSAFINLVEFCREILPEKQAEFFEPWVYSFSYELILQSTRLPLISGFYKLLSITVRNAKKIKYFEGVSPKSLKHSPEDPEKYSCFALFVKFGKEVAVKMKQYKDELLASCLTFLLSLPHNIIELDVRAYVPALQMAFKLGLSYTPLAEVGLNALEEWSIYIDRHVMQPYYKDILPCLDGYLKTSALSDETKNNWEVSALSRAAQKGFNKVVLKHLKKTKNLSSNEAISLEEIRIRVVQMLGSLGGQINKNLLTVTSSDEMMKSYVAWDREKRLSFAVPFREMKPVIFLDVFLPRVTELALTASDRQTKVAACELLHSMVMFMLGKATQMPEGGQGAPPMYQLYKRTFPVLLRLACDVDQVTRQLYEPLVMQLIHWFTNNKKFESQDTVALLEAILDGIVDPVDSTLRDFCGRCIREFLKWSIKQITPQQQEKSPVNTKSLFKRLYSLALHPNAFKRLGASLAFNNIYREFREEESLVEQFVFEALVIYMESLALAHADEKSLGTIQQCCDAIDHLCRIIEKKHVSLNKAKKRRLPRGFPPSASLCLLDLVKWLLAHCGRPQTECRHKSIELFYKFVPLLPGNRSPNLWLKDVLKEEGVSFLINTFEGGGCGQPSGILAQPTLLYLRGPFSLQATLCWLDLLLAALECYNTFIGERTVGALQVLGTEAQSSLLKAVAFFLESIAMHDIIAAEKCFGTGAAGNRTSPQEGERYNYSKCTVVVRIMEFTTTLLNTSPEGWKLLKKDLCNTHLMRVLVQTLCEPASIGFNIGDVQVMAHLPDVCVNLMKALKMSPYKDILETHLREKITAQSIEELCAVNLYGPDAQVDRSRLAAVVSACKQLHRAGLLHNILPSQSTDLHHSVGTELLSLVYKGIAPGDERQCLPSLDLSCKQLASGLLELAFAFGGLCERLVSLLLNPAVLSTASLGSSQGSVIHFSHGEYFYSLFSETINTELLKNLDLAVLELMQSSVDNTKMVSAVLNGMLDQSFRERANQKHQGLKLATTILQHWKKCDSWWAKDSPLETKMAVLALLAKILQIDSSVSFNTSHGSFPEVFTTYISLLADTKLDLHLKGQAVTLLPFFTSLTGGSLEELRRVLEQLIVAHFPMQSREFPPGTPRFNNYVDCMKKFLDALELSQSPMLLELMTEVLCREQQHVMEELFQSSFRRIARRGSCVTQVGLLESVYEMFRKDDPRLSFTRQSFVDRSLLTLLWHCSLDALREFFSTIVVDAIDVLKSRFTKLNESTFDTQITKKMGYYKILDVMYSRLPKDDVHAKESKINQVFHGSCITEGNELTKTLIKLCYDAFTENMAGENQLLERRRLYHCAAYNCAISVICCVFNELKFYQGFLFSEKPEKNLLIFENLIDLKRRYNFPVEVEVPMERKKKYIEIRKEAREAANGDSDGPSYMSSLSYLADSTLSEEMSQFDFSTGVQSYSYSSQDPRPATGRFRRREQRDPTVHDDVLELEMDELNRHECMAPLTALVKHMHRSLGPPQGEEDSVPRDLPSWMKFLHGKLGNPIVPLNIRLFLAKLVINTEEVFRPYAKHWLSPLLQLAASENNGGEGIHYMVVEIVATILSWTGLATPTGVPKDEVLANRLLNFLMKHVFHPKRAVFRHNLEIIKTLVECWKDCLSIPYRLIFEKFSGKDPNSKDNSVGIQLLGIVMANDLPPYDPQCGIQSSEYFQALVNNMSFVRYKEVYAAAAEVLGLILRYVMERKNILEESLCELVAKQLKQHQNTMEDKFIVCLNKVTKSFPPLADRFMNAVFFLLPKFHGVLKTLCLEVVLCRVEGMTELYFQLKSKDFVQVMRHRDDERQKVCLDIIYKMMPKLKPVELRELLNPVVEFVSHPSTTCREQMYNILMWIHDNYRDPESETDNDSQEIFKLAKDVLIQGLIDENPGLQLIIRNFWSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEMTSMSPDYPNPMFEHPLSECEFQEYTIDSDWRFRSTVLTPMFVETQASQGTLQTRTQEGSLSARWPVAGQIRATQQQHDFTLTQTADGRSSFDWLTGSSTDPLVDHTSPSSDSLLFAHKRSERLQRAPLKSVGPDFGKKRLGLPGDEVDNKVKGAAGRTDLLRLRRRFMRDQEKLSLMYARKGVAEQKREKEIKSELKMKQDAQVVLYRSYRHGDLPDIQIKHSSLITPLQAVAQRDPIIAKQLFSSLFSGILKEMDKFKTLSEKNNITQKLLQDFNRFLNTTFSFFPPFVSCIQDISCQHAALLSLDPAAVSAGCLASLQQPVGIRLLEEALLRLLPAELPAKRVRGKARLPPDVLRWVELAKLYRSIGEYDVLRGIFTSEIGTKQITQSALLAEARSDYSEAAKQYDEALNKQDWVDGEPTEAEKDFWELASLDCYNHLAEWKSLEYCSTASIDSENPPDLNKIWSEPFYQETYLPYMIRSKLKLLLQGEADQSLLTFIDKAMHGELQKAILELHYSQELSLLYLLQDDVDRAKYYIQNGIQSFMQNYSSIDVLLHQSRLTKLQSVQALTEIQEFISFISKQGNLSSQVPLKRLLNTWTNRYPDAKMDPMNIWDDIITNRCFFLSKIEEKLTPLPEDNSMNVDQDGDPSDRMEVQEQEEDISSLIRSCKFSMKMKMIDSARKQNNFSLAMKLLKELHKESKTRDDWLVSWVQSYCRLSHCRSRSQGCSEQVLTVLKTVSLLDENNVSSYLSKNILAFRDQNILLGTTYRIIANALSSEPACLAEIEEDKARRILELSGSSSEDSEKVIAGLYQRAFQHLSEAVQAAEEEAQPPSWSCGPAAGVIDAYMTLADFCDQQLRKEEENASVIDSAELQAYPALVVEKMLKALKLNSNEARLKFPRLLQIIERYPEETLSLMTKEISSVPCWQFISWISHMVALLDKDQAVAVQHSVEEITDNYPQAIVYPFIISSESYSFKDTSTGHKNKEFVARIKSKLDQGGVIQDFINALDQLSNPELLFKDWSNDVRAELAKTPVNKKNIEKMYERMYAALGDPKAPGLGAFRRKFIQTFGKEFDKHFGKGGSKLLRMKLSDFNDITNMLLLKMNKDSKPPGNLKECSPWMSDFKVEFLRNELEIPGQYDGRGKPLPEYHVRIAGFDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSACSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYKDWLTKMSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPEAFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHAFGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDPGLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGWEPWM xrcc6-hoxb7 Ku70 coimmunoprecpitates Ku80 and HOXB7 from MCF-7 lysates treated with Ethidium Bromide 606673 N/A 606679 unspecified role unspecified role prey prey N/A 606680 unspecified role unspecified role bait bait N/A 606681 unspecified role unspecified role prey prey association association false false false 3 E The participation of DNA-PK (CS) in this interaction is dependent on the presence of DNA, as it did not interact in the presence of Ethidium Bromide. xrcc5-hoxb7-1 Ku80 coimmunoprecpitates Ku70 and HOXB7 from MCF-7 lysates treated with Ethidium Bromide 606673 N/A 606680 unspecified role unspecified role prey prey N/A 606679 unspecified role unspecified role bait bait N/A 606681 unspecified role unspecified role prey prey association association false false false 3 E The participation of DNA-PK (CS) in this interaction is dependent on the presence of DNA, as it did not interact in the presence of Ethidium Bromide. xrcc6-prkdc Ku70 coimmunoprecipitates Ku80, HoxB7 and DNA-PK (CS) in MCF-7 lysates not treated with Ethidium Bromide 606673 N/A 606680 unspecified role unspecified role bait bait N/A 606682 unspecified role unspecified role prey prey N/A 606681 unspecified role unspecified role prey prey N/A 606679 unspecified role unspecified role prey prey association association false false false 3 E The participation of DNA-PK (CS) in this interaction is dependent on the presence of DNA, as it did not interact in the presence of Ethidium Bromide. xrcc5-prkdc Ku80 coimmunoprecipitates Ku70, HOXB7 and DNA-PK (CS) in MCF-7 lysates not treated with Ethidium Bromide 606673 N/A 606681 unspecified role unspecified role prey prey N/A 606680 unspecified role unspecified role prey prey N/A 606679 unspecified role unspecified role bait bait N/A 606682 unspecified role unspecified role prey prey association association false false false 3 E The participation of DNA-PK (CS) in this interaction is dependent on the presence of DNA, as it did not interact in the presence of Ethidium Bromide. hoxb7-xrcc5-4 GST-HOXB7 pulls down Ku80 and Ku70 from MCF10A extracts 606674 N/A 606680 unspecified role unspecified role prey prey human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line N/A 606681 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 606679 unspecified role unspecified role prey prey human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line association association false false false 3 A PARP was also identified as an interactor, but as the isoform of PARP is not clear, it cannot be added to this interaction. DNA-PK (CS) was also inferred as an interactor but definitely identified and thus has not been added as an interactor. hoxb7-xrcc5-3 GST-HOXB7 pulls down Ku70 and Ku80 from SKBR3 extracts 606674 N/A 606679 unspecified role unspecified role prey prey human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion N/A 606681 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 606680 unspecified role unspecified role prey prey human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion association association false false false 3 A PARP was also identified as an interactor, but as the isoform of PARP is not clear, it cannot be added to this interaction. DNA-PK (CS) was also inferred as an interactor but definitely identified and thus has not been added as an interactor. hoxb7-xrcc5 YFP-HOXB7 coimmunoprecipitates Ku80 and Ku70 in SKBR3 lysates 606675 N/A 606679 unspecified role unspecified role prey prey N/A 606680 unspecified role unspecified role prey prey N/A 606681 unspecified role unspecified role bait bait region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 3 C P09629 transfection hoxb7-xrcc5-2 FLAG-HOXB7 coimmunoprecipitates Ku80 and Ku70 in SKBR3 lysates 606675 N/A 606679 unspecified role unspecified role prey prey N/A 606681 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 606680 unspecified role unspecified role prey prey association association false false false 4 B P09629 transfection xrcc5-hoxb7 YFP-HOXB7 coimmunoprecipitates with Ku80 in SKBR3 lysates 606676 N/A 606681 unspecified role unspecified role prey prey region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 606679 unspecified role unspecified role bait bait physical association physical association false false false P09629 transfection 3 D prkdc-hoxb7 YFP-HOXB7 coimmunoprecipitates with DNA-PK (CS) in SKBR3 lysates 606676 N/A 606682 unspecified role unspecified role bait bait N/A 606681 unspecified role unspecified role prey prey region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false P09629 transfection 3 D hoxb7-g22p1-1 GST-HOXB7 pulls down Ku70, Ku80 and DNA-PK (CS) from SKBR3 extracts 606677 N/A 606679 unspecified role unspecified role prey prey human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line N/A 606682 unspecified role unspecified role prey prey human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line N/A 606681 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 606680 unspecified role unspecified role prey prey human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line association association false false false 3 B PARP was also identified as an interactor, but the isoform of PARP is not clear and thus it could not be added to the interaction. hoxb7-xrcc5-1 FLAG-HOXB7 coimmunoprecipitates Ku70 and Ku80 in CHO lysates 606678 N/A 606680 unspecified role unspecified role prey prey N/A 606679 unspecified role unspecified role prey prey N/A 606681 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4 A P09629; P12956; P13010 transfection As coexpression of Ku70 and Ku80 was required for the association of either Ku subunit with HOXB7, it is possible that Ku70/Ku80 heterodimer formation is a prerequisite for HOXB7 binding.