IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ schlaitz-2007-2 The C. elegans RSA complex localizes protein phosphatase 2A to centrosomes and regulates mitotic spindle assembly. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Schlaitz AL., Srayko M., Dammermann A., Quintin S., Wielsch N., MacLeod I., de Robillard Q., Zinke A., Yates JR., Muller-Reichert T., Shevchenko A., Oegema K., Hyman AA. Chromatin - Epigenetic interactions resulting in chromatin modulation hyman@mpi-cbg.de Cell (0092-8674) 2007 Only protein-protein interactions 6 imex curation Cancer - Interactions investigated in the context of cancer schlaitz-2007-4 The C. elegans RSA complex localizes protein phosphatase 2A to centrosomes and regulates mitotic spindle assembly. caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Schlaitz AL., Srayko M., Dammermann A., Quintin S., Wielsch N., MacLeod I., de Robillard Q., Zinke A., Yates JR., Muller-Reichert T., Shevchenko A., Oegema K., Hyman AA. Chromatin - Epigenetic interactions resulting in chromatin modulation hyman@mpi-cbg.de Cell (0092-8674) 2007 Only protein-protein interactions 6 imex curation Cancer - Interactions investigated in the context of cancer schlaitz-2007-1 The C. elegans RSA complex localizes protein phosphatase 2A to centrosomes and regulates mitotic spindle assembly. caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Schlaitz AL., Srayko M., Dammermann A., Quintin S., Wielsch N., MacLeod I., de Robillard Q., Zinke A., Yates JR., Muller-Reichert T., Shevchenko A., Oegema K., Hyman AA. Chromatin - Epigenetic interactions resulting in chromatin modulation hyman@mpi-cbg.de Cell (0092-8674) 2007 Only protein-protein interactions 6 imex curation Cancer - Interactions investigated in the context of cancer schlaitz-2007-3 The C. elegans RSA complex localizes protein phosphatase 2A to centrosomes and regulates mitotic spindle assembly. caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Schlaitz AL., Srayko M., Dammermann A., Quintin S., Wielsch N., MacLeod I., de Robillard Q., Zinke A., Yates JR., Muller-Reichert T., Shevchenko A., Oegema K., Hyman AA. Chromatin - Epigenetic interactions resulting in chromatin modulation hyman@mpi-cbg.de Cell (0092-8674) 2007 Only protein-protein interactions 6 imex curation Cancer - Interactions investigated in the context of cancer spd5_caeel Spindle-defective protein 5 spd-5 F56A3.4 protein protein caeel Caenorhabditis elegans MEDNSVLNEDSNLEHVEGQPRRSMSQPVLNVEGDKRTSSTSATQQQVLSGAFSSADVRSIPIIQTWEENKALKTKITILRGELQMYQRRYSEAKEASQKRVKEVMDDYVDLKLGQENVQEKMEQYKLMEEDLLAMQSRIETSEDNFARQMKEFEAQKHAMEERIKELELSATDANNTTVGSFRGTLDDILKKNDPDFTLTSGYEERKINDLEAKLLSEIDKVAELEDHIQQLRQELDDQSARLADSENVRAQLEAATGQGILGAAGNAMVPNSTFMIGNGRESQTRDQLNYIDDLETKLADAKKENDKARQALVEYMNKCSKLEHEIRTMVKNSTFDSSSMLLGGQTSDELKIQIGKVNGELNVLRAENRELRIRCDQLTGGDGNLSISLGQSRLMAGIATNDVDSIGQGNETGGTSMRILPRESQLDDLEESKLPLMDTSSAVRNQQQFASMWEDFESVKDSLQNNHNDTLEGSFNSSMPPPGRDATQSFLSQKSFKNSPIVMQKPKSLHLHLKSHQSEGAGEQIQNNSFSTKTASPHVSQSHIPILHDMQQILDSSAMFLEGQHDVAVNVEQMQEKMSQIREALARLFERLKSSAALFEEILERMGSSDPNADKIKKMKLAFETSINDKLNVSAILEAAEKDLHNMSLNFSILEKSIVSQAAEASRRFTIAPDAEDVASSSLLNASYSPLFKFTSNSDIVEKLQNEVSELKNELEMARTRDMRSPLNGSSGRLSDVQINTNRMFEDLEVSEATLQKAKEENSTLKSQFAELEANLHQVNSKLGEVRCELNEALARVDGEQETRVKAENALEEARQLISSLKHEENELKKTITDMGMRLNEAKKSDEFLKSELSTALEEEKKSQNLADELSEELNGWRMRTKEAENKVEHASSEKSEMLERIVHLETEMEKLSTSEIAADYCSTKMTERKKEIELAKYREDFENAAIVGLERISKEISELTKKTLKAKIIPSNISSIQLVCDELCRRLSREREQQHEYAKVMRDVNEKIEKLQLEKDALEHELKMMSSNNENVPPVGTSVSGMPTKTSNQKCAQPHYTSPTRQLLHESTMAVDAIVQKLKKTHNMSGMGPELKETIGNVINESRVLRDFLHQKLILFKGIDMSNWKNETVDQLITDLGQLHQDNLMLEEQIKKYKKELKLTKSAIPTLGVEFQDRIKTEIGKIATDMGGAVKEIRKK rsa2_caeel Regulator of spindle assembly protein 2 Y48A6B.11 rsa-2 protein protein caeel Caenorhabditis elegans MSKIPVFKGSFKSWLAKNDEKPAKSVLLEPKYRDHHEKISFRNKENMEEGEKPVFVSMANPQPTREDYERYDEDRRLKDKARDLRIARRRNSATPEASPSSDQYFTPEPADDEFVTPSTSKKSQKPRSSDATPVSSKPPRYLPRTPLSEQYTSCLSRKMEENFSRMQELMISGHSPHEARQQTIQESSEQLEPRAKVTRSSSQPPPIDTLKPRVPRIESPLVKKTTETPIRRTSYVDTLVATHQIQLQYSDRVVVGVERQMTKLESIKNLAAANRSPIIAEEAKKRRNEAEAVRKLIEVETQNAKKRAVIQELKDRIDKLTQAQLAIHQLVSSQPFSGDPYNQRLLRSIDNWMALPFREFDIQTAREMLELARKMKITIDHFRNVATLHRNSKSLNRSLNTSRKSIAVKINPSSQLNQQSSSDAAPPPSMREASTQMTSRLAESAMTQTSPRRIGVEPLDLSQLLEKHNSSSQTTPPVVEPVLAEVSAEPQRPPVTLSMTAPVSTIAEFDSMLNSISLHNESLETVAEPFSRLKTDISFPSTVEETTPRSGRVSLDSESARRLSAGLSHYLEQVKKERESMEAQESESESMELEIPVVSEVSVTTESENLEEVVSEHSDSKSPETLVASDNGEDSSGGSEDPNATQFEHEIEEHKEPEKLGLIIDPEDEQDETKRFVNHDEFEQSLEEELEPRGNNDSADDSGFLLDNSPAPRLKSIFDNLPPAAASAAVTDTPRVPAEADETTFAGMDMEEYCQREFLKEISPIMVQKAIELQDELRGVDWLTAQDVWQPPSFKDVQMEFDDNFEYFDSFSILIWSAVVDLINKNYLKFGRKMTENEEIAFEAEALKMLQTEHGPESRKSEWCTDVKMSKKLEGMMPMELDYRYDVRRGLPDAEKQKYQWQQVQMTVIAARYANKNLINEANEVYATEKEKLGQMVLESEIDATVTDV p2r31_caeel Serine/threonine-protein phosphatase 2A regulatory subunit rsa-1 rsa-1 Regulator of spindle assembly protein 1 Serine/threonine-protein phosphatase 2A 72kDa regulatory subunit rsa-1 Serine/threonine-protein phosphatase 2A regulatory subunit B'' rsa-1 C25A1.9 protein protein caeel Caenorhabditis elegans MPTDEPSKRKSILPTIPTSLMLKKSNEALSDFERTFNDRVMDIFAENRRIDVEEFKKNAECFLNIIRSNKIDLNWGEGGESRYVTITRLMKILKTSPQSIKDLLPHNTVSNFVKITNYNLTIDITLLEELVRTVIHAEESYIKLLPFSENSTEISSYSLQDFVATHFIPIMIEEPENPVYYTAYAVGTIFFLLGARRRDCVYLKDLLASTLLLQLEECIHAENHCLSPPKIDVFTVAQFRTTLSEFRFLDSQRKGLLAPADLKFFRDGIFNEVFTKRIFEISITYEDGRIDFKAFVDFVTALKFRHTTASAKYHFEILDLKDDGLLDEEEIRSISSFQLQNLPDYVPEDNSVNPEVATAELRDMMRLNQNGITLEEFLANRMNSTFAGFLSNSDDYMKYERREQ pp2a_caeel Serine/threonine-protein phosphatase 2A catalytic subunit let-92 F38H4.9 protein protein caeel Caenorhabditis elegans MAAAPPSADPLDKALIVDVDQWIEQLYECKPLSENQVKTLCEKAKEILEKEPNVQEVRCPVTVCGDVHGQFHDLMELFKMGGKSPDTNYLFMGDYVDRGYYSVETVSLLVCLKIRYKDRVTLLRGNHESRQITQVYGFYDECLRKYGNSNVWKYFTDLFDCFPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRIQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHSNGLTLISRAHQLVMEGYNWSHDRNVVTVFSAPNYCYRCGNQAAMVELDDDLKYSFLQFDPAPRRGEPHVTRRTPDYFL g5edl3_caeel Aurora kinase AIR-1 air-1 K07C11.2 CELE_K07C11.2 protein protein caeel Caenorhabditis elegans MSGKENTAPVIDDQKAEVISLTEDSRPQRVDQAREESCWSLDDFDVGRPLGKGKFGNVFISREKKTKRIIALKVLFKTQLLQLGVSHQLKREIEIQYHLRHPNILTLYGYFHDDKRVFVILDYASRGELFNVLQSQPGHKVNEVIAGRFVRQLANALHYCHSKGVIHRDIKPENLLLDSKLNLKLADFGWSVVADHSKRHTLCGTMDYLAPEMVSNQPHDFNVDIWAIGILLFEMLVGYAPFANQTGDKLIARIKECKIYIPSVVTDGAASLINAIIKKEPQERLPLVDIMAHPWIKEMKQREDIEVPLFISTLTKSSRNNSTANQ g5ede7_caeel tpxl-1 Y39G10AR.12 1C866 CELE_Y39G10AR.12 protein protein caeel Caenorhabditis elegans MSKSQTIQEFDENFFSSFAVPKVKDYSRLSSSPSRDQPCETSYFENHFPSLECDTPVKMQLPKTKRESDDDLPSPTIDNLLSSSSESTVINASQNDNTEEKQQQPIREPLKIVPNEDASASGSGGENQLIEGLEKLSMFAPKAPEERLSQRRRSEQRKKEEEDKRTSVIAPASGTRSRSISTKRSMSREPAAAAPRAPSVPRPPHNSVTRTISAPIANTGPGVITRAMAARVAENVTKTSVRRSVMPLKVAQKTPVAAVKSDAATAGGDRSRDRTRRTNTTASRRSVFPNKPAAAAANTTPIAPPRRSASANKATAPTSVSKLTPKIRPIATANRSSILRMNVVKPTVPAPAPADAQQNKAASRPAVPAGGAARNRQPLRVNPGTPSTTIKKPPVASSVQRQPPTRARSVDRSGQPPISASTPGKASRADLFNRLATPKVTATPTRTSHEGPASSIRKSAVRPVPSYVYSSYKGMPSAGGLHGIRAPSKDRSTQKPPAAASTPQSKQ 2aaa_caeel Probable serine/threonine-protein phosphatase PP2A regulatory subunit F48E8.5 Protein phosphatase PP2A regulatory subunit A paa-1 protein protein caeel Caenorhabditis elegans MSVVEEATDDALYPIAVLIDELRNEDVTLRLNSIRKLSTIALALGVERTRNELIQFLTDTIYDEDEVLLVLAEQLGNFTPLVGGPDHVHCLLLPLENLATVEETVVRDKAVESLRKIADKHSSASLEEHFVPMLRRLATGDWFTSRTSACGLFSVVYPRVSPAIKSELKSMFRTLCRDDTPMVRRAAAAKLGEFAKVFEKTAVIEGLHSSLTDLHVDEQDSVRLLTVESAIAFGTLLDKANKKKLIEPILIELFDDKSWRVRYMVAEKLIEIQNVLGEDMDTTHLVNMYTNLLKDPEGEVRCAATQRLQEFALNLPEDKRQNIICNSLLNVAKELVTDGNQLVKSELAGVIMGLAPLIGKEQTVSELLPIYMQLLNDQTPEVRLNIISSLDKVNEVIGAAQLSTSLLPAIVGLAEDGKWRVRLAIVQFMPLLASQLGQEFFDEKLLPLCLNWLTDHVFSIREASTLIMKELTQKFGGQWASTNIVPKMQKLQKDTNYLQRMTCLFCLNTLSEAMTQEQILKEIMPIVKDLVEDDVPNVRFNAAKSLKRIGKNLTPSTLTSEVKPLLEKLGKDSDFDVRYFSEEAKNSLGL y48a6b_11-spd_5 RSA-2 interacts with SPD-5 (aa 280-1198) in a yeast two-hybrid assay 577154 N/A 577158 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 577159 unspecified role unspecified role bait bait physical association physical association false false false 2 D, Table S1 There are 2 proteins corresponding to Y48A6B.11 (RSA-2). The longer protein Q7YSJ2 has an insertion of SF at position 40-41 compared to the shorter protein (Q9TVY5). y48a6b_11-o02217 RSA-2 interacts with RSA-1 in a yeast two-hybrid assay 577154 N/A 577160 unspecified role unspecified role prey prey N/A 577159 unspecified role unspecified role bait bait physical association physical association false false false 2 D, Table S1 There are 2 proteins corresponding to Y48A6B.11 (RSA-2). The longer protein Q7YSJ2 has an insertion of SF at position 40-41 compared to the shorter protein (Q9TVY5). tpxl_1-air_1 TPXL-1 coimmunoprecipitates AIR-1, RSA-1 and -2, PAA-1 and LET-92 577155 N/A 577161 unspecified role unspecified role prey prey N/A 577160 unspecified role unspecified role prey prey N/A 577162 unspecified role unspecified role prey prey N/A 577163 unspecified role unspecified role bait bait N/A 577159 unspecified role unspecified role prey prey N/A 577164 unspecified role unspecified role prey prey association association false false false Table S2 There are 2 proteins corresponding to Y48A6B.11 (RSA-2). The longer protein Q7YSJ2 has an insertion of SF at position 40-41 compared to the shorter protein (Q9TVY5). There are 2 proteins corresponding to TPXl-1. The longer protein has MPSAGGLH instead of YEKKRWIY at position 476-482 as compared to the shorter protein (Q8WSN6) and extra residues at the C-terminus (483-507). q7ysj2-2aaa RSA-2 coimmunoprecipitates RSA-1, PAA-1 and LET-92 577155 N/A 577164 unspecified role unspecified role prey prey N/A 577161 unspecified role unspecified role prey prey N/A 577159 unspecified role unspecified role bait bait N/A 577160 unspecified role unspecified role prey prey association association false false false Table S2 There are 2 proteins corresponding to Y48A6B.11 (RSA-2). The longer protein Q7YSJ2 has an insertion of SF at position 40-41 compared to the shorter protein (Q9TVY5). c25a1_9-let_92 RSA-1 coimmunoprecipitates with a PP2A complex, SPD-5 and RSA-2 (Y48A6B.11) 577156 N/A 577160 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 577159 unspecified role unspecified role prey prey N/A 577161 unspecified role unspecified role prey prey N/A 577158 unspecified role unspecified role prey prey N/A 577164 unspecified role unspecified role prey prey association association false false false Table 1 There are 2 proteins corresponding to Y48A6B.11 (RSA-2). The longer protein Q7YSJ2 has an insertion of SF at position 40-41 compared to the shorter protein (Q9TVY5). tpxl_1-o02217 RSA-1 and -2 coimmunoprecipitate with TPXl-1 in C. elegans embryo extracts 577157 N/A 577163 unspecified role unspecified role bait bait n/a 577159 unspecified role unspecified role prey prey N/A 577160 unspecified role unspecified role prey prey association association false false false 6 C There are 2 proteins corresponding to Y48A6B.11 (RSA-2). The longer protein Q7YSJ2 has an insertion of SF at position 40-41 compared to the shorter protein (Q9TVY5). There are 2 proteins corresponding to TPXl-1. The longer protein has MPSAGGLH instead of YEKKRWIY at position 476-482 as compared to the shorter protein (Q8WSN6) and extra residues at the C-terminus (483-507).