MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint rho-2006-1 A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis jeholee@unitel.co.kr A human ovary cDNA library was used. FEBS Lett. (0014-5793) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer rho-2006-2 A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis The approximate range for the BTB-PLZF construct was obtained by aligning the primers against the PLZF DNA sequence AF060568 using Align and consulting the information regarding the cds. jeholee@unitel.co.kr A human ovary cDNA library was used. FEBS Lett. (0014-5793) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer rho-2006-3 A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis jeholee@unitel.co.kr A human ovary cDNA library was used. FEBS Lett. (0014-5793) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer rho-2006-4 A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. human-siha Homo sapiens SiHa cervical carcinoma cell line siha SiHa cells huamn cervical carcinoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rho SB., Park YG., Park K., Lee SH., Lee JH. Apoptosis - Interactions involving proteins with a function related to apoptosis jeholee@unitel.co.kr A human ovary cDNA library was used. FEBS Lett. (0014-5793) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer ef1a1_human Elongation factor 1-alpha 1 EEF1A1 EEF1A EF1A LENG7 Eukaryotic elongation factor 1 A-1 Elongation factor Tu Leukocyte receptor cluster member 7 protein protein human Homo sapiens Human MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK zbt16_human Zinc finger and BTB domain-containing protein 16 Zinc finger protein PLZF Promyelocytic leukemia zinc finger protein Zinc finger protein 145 ZBTB16 PLZF ZNF145 protein protein human Homo sapiens Human MDLTKMGMIQLQNPSHPTGLLCKANQMRLAGTLCDVVIMVDSQEFHAHRTVLACTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKAEDLDDLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGTLQPPAGPEEPTLAGGGRHPGVAEVKTEMMQVDEVPSQDSPGAAESSISGGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESAEQVPPPAEAGQAPTGRPEHPAPPPEKHLGIYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKSESRTIGEQCSVCGVELPDNEAVEQHRKLHSGMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFVCDQCGAQFSKEDALETHRQTHTGTDMAVFCLLCGKRFQAQSALQQHMEVHAGVRSYICSECNRTFPSHTALKRHLRSHTGDHPYECEFCGSCFRDESTLKSHKRIHTGEKPYECNGCGKKFSLKHQLETHYRVHTGEKPFECKLCHQRSRDYSAMIKHLRTHNGASPYQCTICTEYCPSLSSMQKHMKGHKPEEIPPDWRIEKTYLYLCYV zbtb16-q8iub0 PLZF interacts with CCS-3 in a yeast two-hybrid assay 500809 n/a 500813 unspecified role unspecified role prey prey N/A 500814 unspecified role unspecified role bait bait physical association physical association false false false zbtb16-q8iub0-1 PLZF interacts with CCS-3 in a yeast two-hybrid assay 500810 n/a 500813 unspecified role unspecified role prey prey N/A 500814 unspecified role unspecified role bait bait region BTB domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false physical association physical association false false false 1 A The PLZF construct containing the BTB domain bound to CCS-3 but those containing the RD2 or ZF domains did not. zbtb16-q8iub0-2 GST-PLZF pulls down CCS-3 in vitro 500811 n/a 500813 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 500814 unspecified role unspecified role bait bait region BTB domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false 1 B zbtb16-q8iub0-3 GST-PLZF coimmunoprecipitates CCS-3 in SiHa cell lysates 500812 n/a 500813 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 500814 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 C Q05516; P68104 transfection