IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hayashida-2005-5 E-cadherin regulates the association between beta-catenin and actinin-4. human-dld1 Human colorectal adenocarcinoma dld1 Human colon adenocarcinoma confocal microscopy confocal microscopy immunostaining immunostaining Hayashida Y., Honda K., Idogawa M., Ino Y., Ono M., Tsuchida A., Aoki T., Hirohashi S., Yamada T. Cancer - Interactions investigated in the context of cancer tyamada@ncc.go.jp Cancer Res. (0008-5472) 2005 Only protein-protein interactions imex curation hayashida-2005-1 E-cadherin regulates the association between beta-catenin and actinin-4. human-colon human colon colon anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hayashida Y., Honda K., Idogawa M., Ino Y., Ono M., Tsuchida A., Aoki T., Hirohashi S., Yamada T. Cancer - Interactions investigated in the context of cancer tyamada@ncc.go.jp Cancer Res. (0008-5472) 2005 Only protein-protein interactions imex curation hayashida-2005-2 E-cadherin regulates the association between beta-catenin and actinin-4. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hayashida Y., Honda K., Idogawa M., Ino Y., Ono M., Tsuchida A., Aoki T., Hirohashi S., Yamada T. Cancer - Interactions investigated in the context of cancer tyamada@ncc.go.jp Cancer Res. (0008-5472) 2005 Only protein-protein interactions imex curation hayashida-2005-3 E-cadherin regulates the association between beta-catenin and actinin-4. human-colon human colon colon anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Hayashida Y., Honda K., Idogawa M., Ino Y., Ono M., Tsuchida A., Aoki T., Hirohashi S., Yamada T. Cancer - Interactions investigated in the context of cancer tyamada@ncc.go.jp Cancer Res. (0008-5472) 2005 Only protein-protein interactions imex curation hayashida-2005-4 E-cadherin regulates the association between beta-catenin and actinin-4. human-colon human colon colon confocal microscopy confocal microscopy immunostaining immunostaining Hayashida Y., Honda K., Idogawa M., Ino Y., Ono M., Tsuchida A., Aoki T., Hirohashi S., Yamada T. Cancer - Interactions investigated in the context of cancer tyamada@ncc.go.jp Cancer Res. (0008-5472) 2005 Only protein-protein interactions imex curation ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL actn4_human Alpha-actinin-4 ACTN4 Non-muscle alpha-actinin 4 protein protein human Homo sapiens Human MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL cadh1_mouse Cadherin-1 Epithelial cadherin Uvomorulin ARC-1 Cdh1 protein protein mouse Mus musculus Mouse MGARCRSFSALLLLLQVSSWLCQELEPESCSPGFSSEVYTFPVPERHLERGHVLGRVRFEGCTGRPRTAFFSEDSRFKVATDGTITVKRHLKLHKLETSFLVRARDSSHRELSTKVTLKSMGHHHHRHHHRDPASESNPELLMFPSVYPGLRRQKRDWVIPPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIERETGWLKVTQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAEGAVPGTSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPVFNPSTYQGQVPENEVNARIATLKVTDDDAPNTPAWKAVYTVVNDPDQQFVVVTDPTTNDGILKTAKGLDFEAKQQYILHVRVENEEPFEGSLVPSTATVTVDVVDVNEAPIFMPAERRVEVPEDFGVGQEITSYTAREPDTFMDQKITYRIWRDTANWLEINPETGAIFTRAEMDREDAEHVKNSTYVALIIATDDGSPIATGTGTLLLVLLDVNDNAPIPEPRNMQFCQRNPQPHIITILDPDLPPNTSPFTAELTHGASVNWTIEYNDAAQESLILQPRKDLEIGEYKIHLKLADNQNKDQVTTLDVHVCDCEGTVNNCMKAGIVAAGLQVPAILGILGGILALLILILLLLLFLRRRTVVKEPLLPPDDDTRDNVYYYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPQYRPRPANPDEIGNFIDENLKAADSDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESDQDQDYDYLNEWGNRFKKLADMYGGGEDD actn4-ctnnb1-2 Actinin-4 and beta-catenin colocalize in DLD-1 cells 669267 N/A 669272 unspecified role unspecified role neutral component neutral component N/A 669273 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3 H-M The expression level of beta-catenin was significantly elevated by the inhibition of E-cadherin expression with siRNA, and beta-catenin and actinin-4 proteins jointly distributed in the bleb-like protrusions formed at the free cell surfaces, as in SW480 cells lacking expression of E-cadherin. In control cells the colocalization mainly took place in the nucleus. actn4-ctnnb1-3 HA-Actinin-4 and beta-catenin colocalize in DLD-1 cells 669267 N/A 669272 unspecified role unspecified role neutral component neutral component N/A 669273 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 4 D-I This interaction occurred when expression of HA-actinin-4 was induced by removal of doxycycline from culture medium of DLD-1 Tet-off ACTN-4 cells. The proteins colocalized at the leading edge of wavy protrusions. ctnnb1-actn4 Coimmunoprecipitation of actinin-4 with beta-catenin is inhibited by expression of E-cadherin 669268 N/A 669274 inhibitor inhibitor prey prey N/A 669273 unspecified role unspecified role prey prey N/A 669272 unspecified role unspecified role bait bait association association false false false 4 C E-cadherin - Transfection with E-cadherin significantly reduced the amount of actinin-4 coimmunoprecipitated with beta-catenin. This indicates that recovery of E-cadherin-mediated cell adhesion inhibits the aberrant interaction between actinin-4 and beta-catenin in SW480 cells. actn4-ctnnb1 Actinin-4 and beta-catenin interact in a yeast two-hybrid assay 669269 N/A 669272 unspecified role unspecified role prey prey n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 669273 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2 H The UniProtKB entry O43707 contains an additional 27 aa at the N-terminus not found in the sequence referenced by the authors for actinin-4, BA24447. BAIT protein BA24447; PREY protein NP_001895 ctnnb1-actn4-1 Beta-catenin coimmunoprecipitates actinin-4 from SW480 lysates 669270 N/A 669272 unspecified role unspecified role bait bait N/A 669273 unspecified role unspecified role prey prey physical association physical association false false false 2 A, B actn4-ctnnb1-1 Actinin-4 and beta-catenin colocalize in protrusions on the free surfaces of SW480 cell clusters 669271 N/A 669272 unspecified role unspecified role neutral component neutral component N/A 669273 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2 E-G Beta-catenin and actinin-4 were colocalized in bleb-like protrusions formed on the free surfaces of SW480 cell clusters. SW480 cells lack expression of E-cadherin. The bleb-like protrusions disappeared when actinin-4 was knocked down and beta-catenin was diffusely distributed in the cytoplasm.