IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ xie-2006-2 The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. human-22rv1 Homo sapiens 22rv1 Human prostate adenocarcinoma cell line Also known as CWR22Rv1 or Rv1. Cells dsplay a NE (neuroendocrine phenotype). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer xie-2006-5 The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer xie-2006-1 The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer xie-2006-3 The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer xie-2006-4 The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer xie-2006-6 The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer xie-2006-9 The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer bmx_human Cytoplasmic tyrosine-protein kinase BMX Bone marrow tyrosine kinase gene in chromosome X protein Epithelial and endothelial tyrosine kinase NTK38 BMX protein protein human Homo sapiens Human MDTKSILEELLLKRSQQKKKMSPNNYKERLFVLTKTNLSYYEYDKMKRGSRKGSIEIKKIRCVEKVNLEEQTPVERQYPFQIVYKDGLLYVYASNEESRSQWLKALQKEIRGNPHLLVKYHSGFFVDGKFLCCQQSCKAAPGCTLWEAYANLHTAVNEEKHRVPTFPDRVLKIPRAVPVLKMDAPSSSTTLAQYDNESKKNYGSQPPSSSTSLAQYDSNSKKIYGSQPNFNMQYIPREDFPDWWQVRKLKSSSSSEDVASSNQKERNVNHTTSKISWEFPESSSSEEEENLDDYDWFAGNISRSQSEQLLRQKGKEGAFMVRNSSQVGMYTVSLFSKAVNDKKGTVKHYHVHTNAENKLYLAENYCFDSIPKLIHYHQHNSAGMITRLRHPVSTKANKVPDSVSLGNGIWELKREEITLLKELGSGQFGVVQLGKWKGQYDVAVKMIKEGSMSEDEFFQEAQTMMKLSHPKLVKFYGVCSKEYPIYIVTEYISNGCLLNYLRSHGKGLEPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDRDLCVKVSDFGMTRYVLDDQYVSSVGTKFPVKWSAPEVFHYFKYSSKSDVWAFGILMWEVFSLGKQPYDLYDNSQVVLKVSQGHRLYRPHLASDTIYQIMYSCWHELPEKRPTFQQLLSSIEPLREKDKH p11309-2 Serine/threonine-protein kinase pim-1 Pim-1L PIM1 protein protein human Homo sapiens Human MPHEPHEPLTPPFSALPDPAGAPSRRQSRQRPQLSSDSPSAFRASRSHSRNATRSHSHSHSPRHSLRHSPGSGSCGSSSGHRPCADILEVGMLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK Initiates from CTG codon. stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD pim1_human Serine/threonine-protein kinase pim-1 PIM1 protein protein human Homo sapiens Human MLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK pim1-bmx-2 Interaction between PIM1 and BMX identified by coimmunoprecipitation 218490 N/A 218497 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 218498 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 4a BMX associates with PIM1L (UniProt identifier /FTId=PRO_0000043349) but not the shorter isoform PIM1S, prodiced by alternative promoter usage (UniProt identifier /FTId=PRO_0000043350). pim1-bmx-3 Interaction between PIM1 and BMX identified by coimmunoprecipitation 218490 N/A 218497 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 218498 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false BMX associates with PIM1L (UniProt identifier /FTId=PRO_0000043349) but not the shorter isoform PIM1S, produced by alternative promoter usage (UniProt identifier /FTId=PRO_0000043350). pim1-bmx-6 Interaction between PIM1 and BMX identified by coimmunoprecipitation 218491 N/A 218497 unspecified role unspecified role prey prey n/a 218498 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 218499 unspecified role unspecified role prey prey association association false false false 4e Phosphorylation of BMX and Tyr-705 of STAT3 bmx-tp53-1 Interaction between BMX/Etk and p53 determined by coimmunoprecipitation 218491 N/A 218497 unspecified role unspecified role prey prey N/A 218500 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false This interaction is disrupted by an assocation between PIM-1(L) and ETK pim1-bmx-1 Interaction between PIM1 and BMX identified by coimmunoprecipitation 218492 N/A 218498 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 218497 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4a, 4b BMX associates with PIM1L (UniProt identifier /FTId=PRO_0000043349) but not the shorter isoform PIM1S, prodiced by alternative promoter usage (UniProt identifier /FTId=PRO_0000043350). Author also performs experiments (Fig. 4B) to demonstrate binding of SH3 domain of BMX (ETK) to PIM1L. However, BMX does not contain an SH3 domain and sequence co-ordinates are not given, so this information has not been added. pim1-bmx-4 Interaction between PIM1 and BMX identified by coimmunoprecipitation 218493 N/A 218501 unspecified role unspecified role bait bait N/A 218497 unspecified role unspecified role prey prey physical association physical association false false false pim1-bmx-5 Interaction between PIM1 and BMX identified by coimmunoprecipitation 218494 N/A 218497 unspecified role unspecified role prey prey N/A 218501 unspecified role unspecified role bait bait physical association physical association false false false pim1-bmx-7 Interaction between PIM1 and BMX identified by coimmunoprecipitation 218495 N/A 218499 unspecified role unspecified role prey prey N/A 218497 unspecified role unspecified role prey prey n/a 218498 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false association association false false false 4e Phosphorylation of BMX and Tyr-705 of STAT3 pim1-bmx-11 Interaction between PIM1 and BMX identified by colocalisation 218496 N/A 218497 unspecified role unspecified role neutral component neutral component region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 218498 unspecified role unspecified role neutral component neutral component n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 3b BMX associates with PIM1L (UniProt identifier /FTId=PRO_0000043349) but not the shorter isoform PIM1S, prodiced by alternative promoter usage (UniProt identifier /FTId=PRO_0000043350).