IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ golubovskay-2005-1 Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Golubovskaya VM., Finch R., Cance WG. Apoptosis - Interactions involving proteins with a function related to apoptosis cance@surgery.ufl.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer golubovskay-2005-2 Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Golubovskaya VM., Finch R., Cance WG. Apoptosis - Interactions involving proteins with a function related to apoptosis cance@surgery.ufl.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer golubovskay-2005-4 Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53. human-bt474 Homo sapiens breast ductal carcinoma cell line bt474 Human breast ductal carcinoma cell line BT474 Human breast ductal carcinoma cell line established from a solid, invasive ductal carcinoma of the breast obtained from a 60-year-old woman. confocal microscopy confocal microscopy immunostaining immunostaining Golubovskaya VM., Finch R., Cance WG. Apoptosis - Interactions involving proteins with a function related to apoptosis Cells were transfected with GFP-FAK constructs or endogenous FAK was visualized using immunostaining. Endogenous p53 was visualized using immunostaining. cance@surgery.ufl.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer golubovskay-2005-3 Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Golubovskaya VM., Finch R., Cance WG. Apoptosis - Interactions involving proteins with a function related to apoptosis cance@surgery.ufl.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer golubovskay-2005-7 Direct Interaction of the N-terminal Domain of Focal Adhesion Kinase with the N-terminal Transactivation Domain of p53 human-cls Homo sapiens fibroblast cell line (GM03318) cls Human fibroblast obtained from patients with Coffin-Lowry Syndrome (CLS). GM03318 (NIGMS Human Genetic Cell Repository) Catalog ID GM03318 (NIGMS Human Genetic Cell Repository) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Golubovskaya VM., Finch R., Cance WG. Apoptosis - Interactions involving proteins with a function related to apoptosis NHF-1 cells were treated or not treated with doxorubicin which caused significant induction of p53 due to stress. cance@surgery.ufl.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer golubovskay-2005-5 Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53. human-bt474 Homo sapiens breast ductal carcinoma cell line bt474 Human breast ductal carcinoma cell line BT474 Human breast ductal carcinoma cell line established from a solid, invasive ductal carcinoma of the breast obtained from a 60-year-old woman. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Golubovskaya VM., Finch R., Cance WG. Apoptosis - Interactions involving proteins with a function related to apoptosis cance@surgery.ufl.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer golubovskay-2005-6 Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Golubovskaya VM., Finch R., Cance WG. Apoptosis - Interactions involving proteins with a function related to apoptosis cance@surgery.ufl.edu J. Biol. Chem. (0021-9258) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD fak1_human Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase Protein phosphatase 1 regulatory subunit 71 p125FAK protein protein human Homo sapiens Human MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH ptk2-tp53 FAK pulls down p53 in vitro 672037 N/A 672044 unspecified role unspecified role prey prey N/A 672045 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false The p53 was baculovirus-expressed but the cell type was not specified. 1 A, 5 p73-alpha, a homolog of p53, did not bind GST-FAK, confirming the specificity of this interaction. tp53-ptk2 p53 pulls down FAK in vitro 672037 N/A 672044 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 672045 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 672056 672054 direct interaction direct interaction false false false 1 C, 4 PYK-2, a FAK family member, did not bind GST-p53, confirming the specificity of this interaction. ptk2-tp53-2 FAK pulls down p53 from 293 lysates 672037 N/A 672045 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 672044 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 2 C The binding of p53 to GST-FAK-NT was higher in lysates from cells overexpressing p53. tp53-ptk2-1 Endogenous p53 coimmunoprecipitates endogenous FAK in 293 cell lysates 672038 N/A 672045 unspecified role unspecified role prey prey N/A 672044 unspecified role unspecified role bait bait physical association physical association false false false 2 A ptk2-tp53-1 Endogenous p53 coimmunoprecipitates with endogenous FAK in 293 cell lysates 672038 N/A 672044 unspecified role unspecified role prey prey N/A 672045 unspecified role unspecified role bait bait physical association physical association false false false 2 A, B Treatment with p53 siRNA reduced the amount of p53 in complex with FAK. ptk2-tp53-3 GFP-FAK and p53 colocalize in BT474 cells 672039 N/A 672044 unspecified role unspecified role neutral component neutral component N/A 672045 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 3 A Colocalization took place in nuclear, perinuclear and cytoplasmic speckles. Q05397 transfection ptk2-tp53-4 Endogenous FAK and p53 colocalize in BT474 cells 672039 N/A 672044 unspecified role unspecified role neutral component neutral component N/A 672045 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3 A Colocalization took place in nuclear, perinuclear and cytoplasmic speckles. tp53-ptk2-2 Endogenous p53 coimmunoprecipitates endogenous FAK in HCT116 cell lysates 672040 N/A 672044 unspecified role unspecified role bait bait N/A 672045 unspecified role unspecified role prey prey physical association physical association false false false 2 D tp53-ptk2-3 Endogenous p53 coimmunoprecipitates endogenous FAK in NHF-1 cell lysates 672041 N/A 672045 unspecified role unspecified role prey prey N/A 672044 unspecified role unspecified role bait bait physical association physical association false false false 2 E Doxorubicin - Complex formation was enhanced in cells treated with doxorubicin 0.5.microg/ml for 24 h. ptk2-tp53-5 FAK coimmunoprecipitates p53 from BT-474 lysates 672042 N/A 672045 unspecified role unspecified role bait bait N/A 672044 unspecified role unspecified role prey prey physical association physical association false false false 3 D This complex was detected in both nuclear and cytoplasmic fractions, but was lower in the nucleus as these cells express lower nuclear p53. ptk2-tp53-6 FAK coimmunoprecipitates p53 from 293 lysates 672043 N/A 672045 unspecified role unspecified role bait bait N/A 672044 unspecified role unspecified role prey prey physical association physical association false false false 3 E This complex was higher in the nuclear fraction in 293 cells as cells express higher levels of nuclear p53.