IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ivanova-2001-1 Regulation of STRA13 by the von Hippel-Lindau tumor suppressor protein, hypoxia, and the UBC9/ubiquitin proteasome degradation pathway. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ivanova AV., Ivanov SV., Danilkovitch-Miagkova A., Lerman MI. Cancer - Interactions investigated in the context of cancer ivanova@mail.ncifcrf.gov The cDNA human fetal kidney Matchmaker library (CLONTECH) was used. J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation ivanova-2001-2 Regulation of STRA13 by the von Hippel-Lindau tumor suppressor protein, hypoxia, and the UBC9/ubiquitin proteasome degradation pathway. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast yeast display yeast display predetermined predetermined participant predetermined Ivanova AV., Ivanov SV., Danilkovitch-Miagkova A., Lerman MI. Cancer - Interactions investigated in the context of cancer ivanova@mail.ncifcrf.gov J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation The cDNA human fetal kidney Matchmaker library (CLONTECH) was used. ivanova-2001-3 Regulation of STRA13 by the von Hippel-Lindau tumor suppressor protein, hypoxia, and the UBC9/ubiquitin proteasome degradation pathway. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ivanova AV., Ivanov SV., Danilkovitch-Miagkova A., Lerman MI. Cancer - Interactions investigated in the context of cancer ivanova@mail.ncifcrf.gov J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation The cDNA human fetal kidney Matchmaker library (CLONTECH) was used. ubc9_human SUMO-conjugating enzyme UBC9 UBE2I p18 SUMO-protein ligase UBC9 UBCE9 Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I RING-type E3 SUMO transferase UBC9 protein protein human Homo sapiens Human MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS bhe40_human Class E basic helix-loop-helix protein 40 BHLHE40 Differentially expressed in chondrocytes protein 1 Enhancer-of-split and hairy-related protein 2 Stimulated by retinoic acid gene 13 protein BHLHB2 DEC1 SHARP2 STRA13 Class B basic helix-loop-helix protein 2 protein protein human Homo sapiens Human MERIPSAQPPPACLPKAPGLEHGDLPGMYPAHMYQVYKSRRGIKRSEDSKETYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHVKALTNLIDQQQQKIIALQSGLQAGELSGRNVETGQEMFCSGFQTCAREVLQYLAKHENTRDLKSSQLVTHLHRVVSELLQGGTSRKPSDPAPKVMDFKEKPSSPAKGSEGPGKNCVPVIQRTFAHSSGEQSGSDTDTDSGYGGESEKGDLRSEQPCFKSDHGRRFTMGERIGAIKQESEEPPTKKNRMQLSDDEGHFTSSDLISSPFLGPHPHQPPFCLPFYLIPPSATAYLPMLEKCWYPTSVPVLYPGLNASAAALSSFMNPDKISAPLLMPQRLPSPLPAHPSVDSSVLLQALKPIPPLNLETKD bhlbh2-ube2i 568311 N/A 568314 unspecified role unspecified role prey prey N/A 568315 unspecified role unspecified role bait bait physical association physical association false false false The authors state that full-length STRA13 is 411 residues long in the method for the yeast two hybrid experiment whereas the Uniprot entry is 412 residues (including the initial Met). Note in Fig. 3 STRA13 is represented as a 412 residue protein. bhlbh2-ube2i-1 568312 N/A 568315 unspecified role unspecified role bait bait N/A 568314 unspecified role unspecified role prey prey physical association physical association false false false The authors state that full-length STRA13 is 411 residues long in the method for the yeast two hybrid experiment whereas the Uniprot entry is 412 residues (including the initial Met). Note in Fig. 3 STRA13 is represented as a 412 residue protein. bhlbh2-ube2i-2 568313 N/A 568315 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 568314 unspecified role unspecified role prey prey physical association physical association false false false The authors state that full-length STRA13 is 411 residues long in the method for the yeast two hybrid experiment whereas the Uniprot entry is 412 residues (including the initial Met). Note in Fig. 3 STRA13 is represented as a 412 residue protein. 3