IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ingley-2000-4 HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. mouse-j2e Mouse fetal liver cells j2e Murine fetal liver cell line In vitro infection of murine fetal liver cells with a retrovirus containing v-raf and v-myc oncogenes has produced continuous lines of immature erythroid cells that are leukemogenic. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2 pklinken@cyllene.uwa.edu.au J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer ingley-2000-1 HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid full dna sequence full identification by DNA sequencing full dna sequence Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. pklinken@cyllene.uwa.edu.au J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer ingley-2000-2 HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 1B,C pklinken@cyllene.uwa.edu.au J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer ingley-2000-3 HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 1D,E pklinken@cyllene.uwa.edu.au J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer ingley-2000-5 HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. mouse-c11 p53-/- mouse fetal liver cells c11 p53-/- mouse fetal liver cells Cells were p53 negative and were transformed with a myc-raf virus. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2A pklinken@cyllene.uwa.edu.au J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer ingley-2000-6 HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. mouse-c24 p53-/- mouse fetal liver cells c24 p53-/- mouse fetal liver cells Cells were p53 negative and were transformed with a myc-raf virus. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2A pklinken@cyllene.uwa.edu.au J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer ingley-2000-7 HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. mouse-erythroleukemia Mus musculus me17-1 Murine erythroid cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2A pklinken@cyllene.uwa.edu.au J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer ingley-2000-8 HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. mouse-me17 Murine erythroid cell line me17 Murine erythroid cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2A pklinken@cyllene.uwa.edu.au J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer ingley-2000-9 HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. mouse-j2e Mouse fetal liver cells j2e Murine fetal liver cell line In vitro infection of murine fetal liver cells with a retrovirus containing v-raf and v-myc oncogenes has produced continuous lines of immature erythroid cells that are leukemogenic. imaging technique imaging technique microscopy immunostaining immunostaining Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2D pklinken@cyllene.uwa.edu.au J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer hcls1_mouse Hematopoietic lineage cell-specific protein Hcls1 Hs1 Hematopoietic cell-specific LYN substrate 1 LckBP1 protein protein mouse Mus musculus Mouse MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDILKKKELESGPKASHGYGGRFGVERDRMDKSAVGHEYVADVEKHSSQTDAARGFGGKYGVERDRADKSAVGFDYKGEVEKHASQKDYSHGFGGRYGVEKDKRDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGARGLKAKFESLAEEKRKREEEEKAQQMARQQQERKAVVKMSREVQQPSMPVEEPAAPAQLPKKISSEVWPPAESHLPPESQPVRSRREYPVPSLPTRQSPLQNHLEDNEEPPALPPRTPEGLQVVEEPVYEAAPELEPEPEPDYEPEPETEPDYEDVGELDRQDEDAEGDYEDVLEPEDTPSLSYQAGPSAGAGGAGISAIALYDYQGEGSDELSFDPDDIITDIEMVDEGWWRGQCRGHFGLFPANYVKLL lyn_mouse Tyrosine-protein kinase Lyn Lyn V-yes-1 Yamaguchi sarcoma viral related oncogene homolog p53Lyn p56Lyn protein protein mouse Mus musculus Mouse MGCIKSKRKDNLNDDEVDSKTQPVRNTDRTIYVRDPTSNKQQRPVPEFHLLPGQRFQTKDPEEQGDIVVALYPYDGIHPDDLSFKKGEKMKVLEEHGEWWKAKSLSSKREGFIPSNYVAKVNTLETEEWFFKDITRKDAERQLLAPGNSAGAFLIRESETLKGSFSLSVRDYDPMHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKHYQKQSDGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKKLGAGQFGEVWMGYYNNSTKVAVKTLKPGTMSVQAFLEEANLMKTLQHDKLVRLYAVVTKEEPIYIITEFMAKGSLLDFLKSDEGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCFTIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMSALSQGYRMPRMENCPDELYDIMKMCWKEKAEERPTFDYLQSVLDDFYTATEGQYQQQP lck_mouse Proto-oncogene tyrosine-protein kinase LCK Lck Lsk-t Lymphocyte cell-specific protein-tyrosine kinase p56-LCK Leukocyte C-terminal Src kinase protein protein mouse Mus musculus Mouse MGCVCSSNPEDDWMENIDVCENCHYPIVPLDSKISLPIRNGSEVRDPLVTYEGSLPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHDLVRHYTNASDGLCTKLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHPRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLNVNKLLDMAAQIAEGMAFIEEQNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYHLMMLCWKERPEDRPTFDYLRSVLDDFFTATEGQYQPQP fyn_mouse Tyrosine-protein kinase Fyn p59-Fyn Fyn Proto-oncogene c-Fyn protein protein mouse Mus musculus Mouse MGCVQCKDKEAAKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQGFLEDYFTATEPQYQPGENL lyn-hs1-11 Interaction between mouse Lyn and Hs1 detected by coIP 363837 N/A 363846 unspecified role unspecified role prey prey N/A 363847 unspecified role unspecified role bait bait physical association physical association false false false hs1-lyn-3 Interaction between mouse Hs1 and Lyn detected by coIP 363837 N/A 363847 unspecified role unspecified role prey prey N/A 363846 unspecified role unspecified role bait bait physical association physical association false false false Maximal coimmunoprecipitation of Lyn occurred between 15 and 30 minutes of erythropoietin stimulation. Erythropoietin. hs1-lck-1 Interaction between mouse Hs1 and Lck detected by coIP 363837 N/A 363846 unspecified role unspecified role prey prey N/A 363848 unspecified role unspecified role bait bait physical association physical association false false false hs1-fyn-1 Interaction between mouse Hs1 and Fyn detected by coIP 363837 N/A 363849 unspecified role unspecified role bait bait N/A 363846 unspecified role unspecified role prey prey physical association physical association false false false lyn-hs1-2 Interaction between mouse Lyn and Hs1 detected by Y2H 363838 N/A 363846 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 363847 unspecified role unspecified role bait bait physical association physical association false false false hs1-lyn-2 Interaction between mouse Hs1 and Lyn detected by GST pulldown 363839 N/A 363847 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) N/A 363846 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false lyn-hs1-7 Interaction between mouse Lyn and Hs1 detected by beta galactosidase 363840 N/A 363847 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 363846 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false lyn-hs1-12 Interaction between mouse Lyn and Hs1 detected by coIP 363841 N/A 363846 unspecified role unspecified role prey prey N/A 363847 unspecified role unspecified role bait bait physical association physical association false false false lyn-hs1-13 Interaction between mouse Lyn and Hs1 detected by coIP 363842 N/A 363846 unspecified role unspecified role prey prey N/A 363847 unspecified role unspecified role bait bait physical association physical association false false false lyn-hs1-14 Interaction between mouse Lyn and Hs1 detected by coIP 363843 N/A 363846 unspecified role unspecified role prey prey N/A 363847 unspecified role unspecified role bait bait physical association physical association false false false lyn-hs1-15 Interaction between mouse Lyn and Hs1 detected by coIP 363844 N/A 363846 unspecified role unspecified role prey prey N/A 363847 unspecified role unspecified role bait bait physical association physical association false false false hs1-lyn-4 Colocalization of mouse Hs1 and Lyn detected by confocal microscopy 363845 N/A 363847 unspecified role unspecified role neutral component neutral component N/A 363846 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false