IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ siller-2006-1 The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Siller KH., Cabernard C., Doe CQ. BioCreative - Critical Assessment of Information Extraction systems in Biology cdoe@uoregon.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation siller-2006-2 The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Siller KH., Cabernard C., Doe CQ. BioCreative - Critical Assessment of Information Extraction systems in Biology cdoe@uoregon.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation siller-2006-3 The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. drome-embryonic_neuroblast Drosophila melanogaster embryonic_neuroblast CNS progenitor cells fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Siller KH., Cabernard C., Doe CQ. BioCreative - Critical Assessment of Information Extraction systems in Biology cdoe@uoregon.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation q9vb22_drome pins raps Dmel\CG5692 PINS Pins Rad Raps rad rapsyn Dmel_CG5692 CG5692 RAPS dLGN dPins protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSLSASAENVSSLGLGSGGGGTTSHDGNSQQGSGSDGGSSMCLELALEGERLCKAGDCRAGVAFFQAAIQAGTEDLRTLSAIYSQLGNAYFYLGDYNKAMQYHKHDLTLAKSMNDRLGEAKSSGNLGNTLKVMGRFDEAAICCERHLTLARQLGDRLSEGRALYNLGNVYHAKGKHLGQRNPGKFGDDVKEALTRAVEFYQENLKLMRDLGDRGAQGRACGNLGNTYYLLGDFQAAIEHHQERLRIAREFGDRAAERRANSNLGNSHIFLGQFEDAAEHYKRTLALAVELGEREVEAQSCYSLGNTYTLLHEFNTAIEYHNRHLAIAQELGDRIGEARACWSLGNAHSAIGGHERALKYAEQHLQLAKELHDPVGESTARVNISDLRKLLGMPDSEPSPTEEEARSTASDHSASGNQSDGSENSQGRMVRVRRQSMEQLDLIKITPDGKRMQEEKLRAQATRKAKDDDFFEMLSRSQSKRMDDQRCSIKVNPAGAPAVATGATRKPLVQQNSLFVDPTNLPGLKSPSSANPSAIGHGPLARSATTTQQPDDDFLDMLMRCQGSRLEEQRSELPRPNVTMDAEAEAPPRSVPEAAVPGAPRGQTGRGATVPDEDFFSLIMKVQSGRMEDQRASIPFRNANNNNNSRSNNNGSAGGAGK q8ir55_drome Dmel\CG12047 KS63 Mud NuMA mud NuMa CG12047 Dmel_CG12047 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDTRSWRKVLLQWIGECHFIESNYITLEQSDLDSFFSVFIQKIQETENVKGKNELQDQPTEQSPLVQEFLAHNYPEFIAQQDDKEVDLPLDCLYVYTLLLHYSCVKKPSLFFHNICNKLPELTQTCIASFFRETVDRLLTREYLSQAIANVAVVYRQGVSTSVSPCLPSSSLSPDPRSDDAPCPSTPSSSSSQPSSSTPQLRNHREQLRLNGCEMPPPSTPKTELLEQRTKELRGIRTQLEVVRYEKALLEEQQMEKDELIKVLNKEKMMAKMELEKLRNVKLTEEHHDNESHHIMPYEFEHMKGCLLKEIGLKESLIAEITDKLHDLRVENSELSEKLNLAGKRLLEYTDRIRFLESRVDDLTRIVSSRDVMISSLESDKQELDKCLKEARDDLHNRIEVLNASSDLLDCSLSPNTTPENLASSVIDKQLREKEHENAELKEKLLNLNNSQRELCQALSSFLQKHNIDHEFPVEWTSSSLLSTISAIESKFVNTLEKSTQMKKECDVQSVCVEKLLEKCKLLSVSLGCQPKELDGFEATIPEAMESGFESSRECETILSCCHMKVVDIASKNNDLELDNERLNDKCAELKSIIDRGDQHLADINLQLIEKEKQIKDVGAEIQELRKRNINLENMLSQIADKEASAASHAQHLKQCGELLRAKYEVCRNELIAKNAAQDELVRMMMVPDGETLNGRVRQLIDLEMMHDEHNKMYAQMLKQLNELSAKHDNMTHSHLDFVKRTEIELETKNAQIMAFDEHNNHFDRFLTRIFTLLRSRNCPKSTTMGSATNFLESMHIEKRFENIEMLIEGQLLSADDLKRELDDLRSKNEELAKQNINGIIKRNKFITSLEVNTEKVKQYITDLEEEAFKRKQKVVQLENTLSKEQSNAKEMAQRLDIAQQEIKDYHVEAIRFINTIRDRLQQDFNGVNTPQQLGTCMTEFLKMYDQMEVRYEESSSLVEKLTESQAKLEMQVAELQVELENKDTNQHSGALIKQLNDTIQNLEKVNAKLSEDNTVSHTVHSKLNESLLKAQKELDLRAKIIENLEASERNLSMKLCELKDLKNKLKSSDEKIAQIKETYEEQIKALQAKCDMEAKKNEHLERNQNQSLTQLKEDALENCVLMSTKLEELQAKLQEGQQLVDSQKLELDMNRKELALVKSAYEAQTKLSDDLQRQKESGQQLVDNLKVELEKERKELAHVNSAIGAQTKLSDDLECQKESGQQLVDNLKVELEKERKELAQVKSVIEAQTKLSDDLQREKESAQQLVDNLKVELDKERKELAQVNSAFEAQTKLSDDLQRQKESAQQLVDNLKVELDKERKELAQVNSAFEAQTKLSDDLQREKESAQQLVDNLKVELDKERKELAQVKSVIEAQTKLSDDLQRQKESAQQLVDNLKVELDKERKELAKVKSVIEAQTKLSDDLQRQKESAQQLEAQTKLSDDLQRQKESAQQLVDNLKVELDKERKELAQVKSVIEAQTKLSDDLQRQKESAQQLVDNLKMELDKERKELAQVKSAIGAQTKLSDDLECQKESVQQLVDNLKVELEKERKELAKVNSAFEAQTKLSDDLKLQKEDAQREVFLVKERLVKEKREFEVKLATLEDIIETLEMRCTQMEEERATAYEQINKLENRCQEKDNVKSSQLQVETFKVECLHHQLKSEMATHNSLVEDLNRKLAEKVSKLDFVQSRLMTEIAEHNQVKDQLAQITDIPKVVELQHRLEAETAEREEAQNKLAVVTGRLDEITRELDNARLEHGAQILRMEETAREVGNKNAELCELIEFYRNRVEALERLLLASNQELEELNSIQSNQAEGVRDLGDTYSAAEGRQTESDQDKERYQKLALDCKILQAKYRDAKDEIKRCEKKIKDQRLEMEGKLEKMKNKMRSLYTAEVTRMKEKQERDAAKSASELEALTAQNAKYEEHTRKLSNQIVRLNEKILEQQKQHAIISTNLRHLQMQPISETKPSSTTLTVSSSSSAPNDDWQPFKRPNVPSSNLAMEDEEGEVFNNTYLTDLKLGRVPADMTAEELIYRNSLQPPHLKSTYAAQYDLGSQDEDLKDGPHSLDDSMSALLSSSSTGTRKKSMGTHYKRPGPPTPSKNGGRLSFGSSEPPREILREFGDHNNTSKTPARFKFLTQRFSVGSSGLPRDEPIPQLPRRKRPNLLTGIHRRRLRHAVDIFCTSTPRKSRSYYDQQRMIGASDADKSEAVETEDEVIEVEPDAVPELEPLEDADQQGTPHLSTAALLALTKGNTRRLTGTTKSKKGRVSLSLHGNIFAKSRPASFVAGKRVQLRRKLRRDRIMGRFDEARHLDQMRLSHSAQAAKSPENNNYSLHNRNEEEHLPSENNVMGKTVVLVGKRRLSPVVSTTFNVEERSETSLLQQQFEHENCVTWAMHGGESVMMEETQNDWYFEQLCKETESTAPFQLQPLDYKPLDEEQVEPKFTQLVAASCSNITTTSCATNMTGASSCTVYSIGSVHMQPLPQINITYVQQPSDLQQRRPTRHRSLMAQCRRFLRHLSLGQRLVMGFALLVMVGLSLHLADKLVLVITGILSGMGLVFLTYSCPGRN mud-raps-1 Interaction of MUD with RAPS demonstrated by pulldown 505461 N/A 505464 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 505465 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) 505471 505468 direct interaction direct interaction false false false 1C mud-raps-2 Interaction of MUD with RAPS demonstrated by coimmunoprecipitation 505462 N/A 505464 unspecified role unspecified role bait bait N/A 505465 unspecified role unspecified role prey prey physical association physical association false false false 1D mud-raps-3 Interaction of MUD with RAPS demonstrated by coimmunoprecipitation 505463 N/A 505465 unspecified role unspecified role neutral component neutral component N/A 505464 unspecified role unspecified role neutral component neutral component physical association physical association false false false 2 & 3