IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rastogi-2006-4 Camptothecin induces nuclear export of prohibitin preferentially in transformed cells through a CRM-1-dependent mechanism. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rastogi S., Joshi B., Fusaro G., Chellappan S. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were treated with a range of chemicals camptothecin(30 micro molar), tamoxifen (10 micro molar), taxol (10nM), 5-fluorouracil (2 micro molar), etopside (30 micro molar), adriamycin (2nM), or cisplatin (20 micro molar) for 4 hours prior to immunostaining. Chellasp@moffitt.usf.edu J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Apoptosis - Interactions involving proteins with a function related to apoptosis rastogi-2006-5 Camptothecin induces nuclear export of prohibitin preferentially in transformed cells through a CRM-1-dependent mechanism. human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. confocal microscopy confocal microscopy immunostaining immunostaining Rastogi S., Joshi B., Fusaro G., Chellappan S. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were treated with a range of chemicals camptothecin(30 micro molar), tamoxifen (10 micro molar), taxol (10nM), 5-fluorouracil (2 micro molar), etopside (30 micro molar), adriamycin (2nM), or cisplatin (20 micro molar) for 4 hours prior to immunostaining. Chellasp@moffitt.usf.edu J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Apoptosis - Interactions involving proteins with a function related to apoptosis rastogi-2006-1 Camptothecin induces nuclear export of prohibitin preferentially in transformed cells through a CRM-1-dependent mechanism. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells confocal microscopy confocal microscopy immunostaining immunostaining Rastogi S., Joshi B., Fusaro G., Chellappan S. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were treated with a range of chemicals camptothecin(30 micro molar), tamoxifen (10 micro molar), taxol (10nM), 5-fluorouracil (2 micro molar), etopside (30 micro molar), adriamycin (2nM), or cisplatin (20 micro molar) for 4 hours prior to immunostaining. Chellasp@moffitt.usf.edu J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Apoptosis - Interactions involving proteins with a function related to apoptosis rastogi-2006-2 Camptothecin induces nuclear export of prohibitin preferentially in transformed cells through a CRM-1-dependent mechanism. human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour confocal microscopy confocal microscopy immunostaining immunostaining Rastogi S., Joshi B., Fusaro G., Chellappan S. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were treated with a range of chemicals camptothecin(30 micro molar), tamoxifen (10 micro molar), taxol (10nM), 5-fluorouracil (2 micro molar), etopside (30 micro molar), adriamycin (2nM), or cisplatin (20 micro molar) for 4 hours prior to immunostaining. Chellasp@moffitt.usf.edu J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Apoptosis - Interactions involving proteins with a function related to apoptosis rastogi-2006-3 Camptothecin induces nuclear export of prohibitin preferentially in transformed cells through a CRM-1-dependent mechanism. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells confocal microscopy confocal microscopy immunostaining immunostaining Rastogi S., Joshi B., Fusaro G., Chellappan S. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were treated with a range of chemicals camptothecin(30 micro molar), tamoxifen (10 micro molar), taxol (10nM), 5-fluorouracil (2 micro molar), etopside (30 micro molar), adriamycin (2nM), or cisplatin (20 micro molar) for 4 hours prior to immunostaining. Chellasp@moffitt.usf.edu J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Apoptosis - Interactions involving proteins with a function related to apoptosis phb_human Prohibitin PHB PHB1 protein protein human Homo sapiens Human MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ xpo1_human Exportin-1 CRM1 XPO1 Chromosome region maintenance 1 protein homolog protein protein human Homo sapiens Human MPAIMTMLADHAARQLLDFSQKLDINLLDNVVNCLYHGEGAQQRMAQEVLTHLKEHPDAWTRVDTILEFSQNMNTKYYGLQILENVIKTRWKILPRNQCEGIKKYVVGLIIKTSSDPTCVEKEKVYIGKLNMILVQILKQEWPKHWPTFISDIVGASRTSESLCQNNMVILKLLSEEVFDFSSGQITQVKSKHLKDSMCNEFSQIFQLCQFVMENSQNAPLVHATLETLLRFLNWIPLGYIFETKLISTLIYKFLNVPMFRNVSLKCLTEIAGVSVSQYEEQFVTLFTLTMMQLKQMLPLNTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHDQLIEKRLNLRETLMEALHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDVPPRRQLYLPMLFKVRLLMVSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTERIMTEKLHNQVNGTEWSWKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAIIASNIMYIVGQYPRFLRAHWKFLKTVVNKLFEFMHETHDGVQDMACDTFIKIAQKCRRHFVQVQVGEVMPFIDEILNNINTIICDLQPQQVHTFYEAVGYMIGAQTDQTVQEHLIEKYMLLPNQVWDSIIQQATKNVDILKDPETVKQLGSILKTNVRACKAVGHPFVIQLGRIYLDMLNVYKCLSENISAAIQANGEMVTKQPLIRSMRTVKRETLKLISGWVSRSNDPQMVAENFVPPLLDAVLIDYQRNVPAAREPEVLSTMAIIVNKLGGHITAEIPQIFDAVFECTLNMINKDFEEYPEHRTNFFLLLQAVNSHCFPAFLAIPPTQFKLVLDSIIWAFKHTMRNVADTGLQILFTLLQNVAQEEAAAQSFYQTYFCDILQHIFSVVTDTSHTAGLTMHASILAYMFNLVEEGKISTSLNPGNPVNNQIFLQEYVANLLKSAFPHLQDAQVKLFVTGLFSLNQDIPAFKEHLRDFLVQIKEFAGEDTSDLFLEEREIALRQADEEKHKRQMSVPGIFNPHEIPEEMCD p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD phb-tp53-3 Coimmunoprecipitation of Prohibitin and CRM-1 protein 504239 N/A 504244 unspecified role unspecified role bait bait N/A 504245 unspecified role unspecified role prey prey physical association physical association false false false Figure 8 Very little CRM-1 associated with prohibitin in MCF-10A cells which were derived from normal mammary epithelial cells. tp53-phb- Coimmunoprecipitation of CRM-1 protein and Prohibitin 504239 N/A 504244 unspecified role unspecified role prey prey N/A 504245 unspecified role unspecified role bait bait physical association physical association false false false Figure 8 Very little CRM-1 associated with prohibitin in MCF-10A cells which were derived from normal mammary epithelial cells. phb-tp53-4 Colocalisation of Prohibitin and p53 protein 504240 N/A 504246 unspecified role unspecified role neutral component neutral component N/A 504244 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 1A Figure 4A The majority of prohibitin and p53 were found to be localized in the nucleus of MCF-7 cells. Treatment of the cells with 30micro molar camptothecin for 4 h led to the translocation of both proteins to the cytoplasmic compartment. Treatment of cells with other apoptotic agents (taxol, tamoxifen, 5-fluorouracil, doxorubicin, etoposide or cisplatin) failed to induce translocation of proteins from nuclear to cytoplasmic compartment. Translocation of proteins from nuclear to cytosolic compartments was not observed in MCF-10A cells which were from normal mammary epithelial cells. phb-tp53 Colocalisation of Prohibitin and p53 protein 504241 N/A 504244 unspecified role unspecified role neutral component neutral component N/A 504246 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 1A Figure 4A The majority of prohibitin and p53 were found to be localized in the nucleus of MCF-7 cells. Treatment of the cells with 30micro molar camptothecin for 4 h led to the translocation of both proteins to the cytoplasmic compartment. Treatment of cells with other apoptotic agents (taxol, tamoxifen, 5-fluorouracil, doxorubicin, etoposide or cisplatin) failed to induce translocation of proteins from nuclear to cytoplasmic compartment. Translocation of proteins from nuclear to cytosolic compartments was not observed in MCF-10A cells which were from normal mammary epithelial cells. phb-tp53-1 Colocalisation of Prohibitin and p53 protein 504242 N/A 504244 unspecified role unspecified role neutral component neutral component N/A 504246 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 1B The majority of prohibitin and p53 were found to be localized in the nucleus of t47d cells. Treatment of the cells with 30micro molar camptothecin for 4 h led to the translocation of both proteins to the cytoplasmic compartment. Treatment of cells with other apoptotic agents (taxol, tamoxifen, 5-fluorouracil, doxorubicin, etoposide or cisplatin) failed to induce translocation of proteins from nuclear to cytoplasmic compartment. Translocation of proteins from nuclear to cytosolic compartments was observed in fibroblast cells identified as the WI-38 cell line that was transformed with SV40 large T-antigen (WI-38-VA13). phb-tp53-2 Colocalisation of Prohibitin and p53 protein 504243 N/A 504246 unspecified role unspecified role neutral component neutral component N/A 504244 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 2 The majority of prohibitin and p53 were found to be localized in the nucleus of MCF-7 cells. Treatment of the cells with 30micro molar camptothecin for 4 h did not lead to the translocation of both proteins to the cytoplasmic compartment.