IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yeh-2006-3 A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology yeh-2006-1 A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology yeh-2006-2 A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology yeh-2006-4 A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology yeh-2006-5 A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells protein kinase assay protein kinase assay predetermined predetermined participant predetermined Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology yeh-2006-6 A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells in gel kinase assay in-gel kinase assay in gel kinase assay predetermined predetermined participant predetermined Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology akt1_human RAC-alpha serine/threonine-protein kinase AKT1 PKB RAC RAC-PK-alpha Protein kinase B Proto-oncogene c-Akt Protein kinase B alpha protein protein human Homo sapiens Human MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA bcl10_human B-cell lymphoma/leukemia 10 BCL10 CIPER CLAP Cellular homolog of vCARMEN Mammalian CARD-containing adapter molecule E10 Cellular-E10 CARD-like apoptotic protein B-cell CLL/lymphoma 10 CED-3/ICH-1 prodomain homologous E10-like regulator CARD-containing molecule enhancing NF-kappa-B protein protein human Homo sapiens Human MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGESSTTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANSSEMFLPLRSRTVSRQ bcl3_human B-cell lymphoma 3 protein BCL3 BCL4 D19S37 Proto-oncogene BCL3 protein protein human Homo sapiens Human MPRCPAGAMDEGPVDLRTRPKAAGLPGAALPLRKRPLRAPSPEPAAPRGAAGLVVPLDPLRGGCDLPAVPGPPHGLARPEALYYPGALLPLYPTRAMGSPFPLVNLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSPPAFLPFAGVLRGPGRPVPPSPAPGGS bcl10_human_gene Human Bcl10 gene gene gene human Homo sapiens Human tf65_human Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit RELA NFKB3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein human Homo sapiens Human MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS ikba_human_gene NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 gene gene human Homo sapiens Human nfkbia_human Human NFKBIA (I-kappa-B alpha) gene dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human bcl10-akt1-1 Interaction between BCL10 and Ak1 demonstrated by coimmunoprecipitation 491682 N/A 491688 unspecified role unspecified role prey prey N/A 491689 unspecified role unspecified role bait bait physical association physical association false false false 5B TNFalpha bcl10-akt1-2 Interaction between BCL10 and Ak1 demonstrated by coimmunoprecipitation 491682 N/A 491688 unspecified role unspecified role bait bait N/A 491689 unspecified role unspecified role prey prey physical association physical association false false false 5B TNFalpha bcl10-bcl3-1 Interaction between BCL3 and BCL10 demonstrated by coimmunoprecipitation 491682 N/A 491689 unspecified role unspecified role prey prey N/A 491690 unspecified role unspecified role bait bait physical association physical association false false false 7D TNFalpha bcl10-bcl3-2 Interaction between BCL3 and BCL10 demonstrated by coimmunoprecipitation 491682 N/A 491690 unspecified role unspecified role prey prey N/A 491689 unspecified role unspecified role bait bait physical association physical association false false false 7D TNFalpha bcl10dna-rela-1 Interaction between BCL10 gene and TP65 demonstrated by Ch-IP 491683 N/A 491691 unspecified role unspecified role prey prey N/A 491692 unspecified role unspecified role bait bait association association false false false 3A TNFalpha rela-nfkbia-9 Interaction between I-kappa-B gene and TP65 demonstrated by Ch-IP 491683 n/a 491693 unspecified role unspecified role prey prey N/A 491692 unspecified role unspecified role bait bait association association false false false 3A TNFalpha bcl10dna-rela-2 Interaction between BCL10 gene and TP65 demonstrated by EMSA 491684 N/A 491691 unspecified role unspecified role neutral component neutral component region -270 to -250 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 491692 unspecified role unspecified role neutral component neutral component human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association false false false 3B, 3C TNFalpha Interaction competed out using unlabelled probe nfkbiadna-rela-3 Interaction between I-kappa-B gene and TP65 demonstrated by EMSA 491684 N/A 491694 unspecified role unspecified role neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false kappab region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 491692 unspecified role unspecified role neutral component neutral component human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association false false false 3B TNFalpha bcl10-akt1-3 Interaction between BCL10 and Ak1 demonstrated by His-pulldown 491685 N/A 491689 unspecified role unspecified role bait bait c-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 491688 unspecified role unspecified role prey prey human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association false false false 6A bcl10-bcl3-3 Interaction between BCL3 and BCL10 demonstrated by His pulldown 491685 N/A 491689 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O95999:p.Ser218Asp mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O95999:p.Ser231Asp mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false N/A 491690 unspecified role unspecified role prey prey human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association false false false 7E TNFalpha bcl10-akt1-4 Phosphorylation of BCL10 by Akt1 491686 N/A 491689 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 491688 enzyme enzyme unspecified role unspecified role human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association false false false 6B Phosphorylation of BCL10 at Ser-218 and Ser-231 akt1-akt1-3 Autophosphorylation of Akt1 observed in in-gel kinase assay 491687 n/a 491688 putative self putative self putative self putative self human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells phosphorylation phosphorylation reaction phosphorylation false true false 6D