IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ boncela-2006-3 Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] facs fluorescence-activated cell sorting FACS Flow cytometry facs immunostaining immunostaining Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation boncela-2006-4 Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells facs fluorescence-activated cell sorting FACS Flow cytometry facs immunostaining immunostaining Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation boncela-2006-1 Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. human-endothelium Homo sapiens endothelail cells/endothelium endothelium Endothelial cells [endothelium] confocal microscopy confocal microscopy predetermined predetermined participant predetermined Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation boncela-2006-2 Binding of PAI-1 to endothelial cells stimulated by thymosin beta4 and modulation of their fibrinolytic potential. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa predetermined predetermined participant predetermined Boncela J., Smolarczyk K., Wyroba E., Cierniewski CS. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells were preincubated with Thymosin beta4 (40nm) for 24 hours before Immunolocalisation and activated cell sorting was used to detect the protein interaction. cciern@zdn.am.lodz.pl J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation a1ag1_human Alpha-1-acid glycoprotein 1 Orosomucoid-1 ORM1 AGP1 protein protein human Homo sapiens Human MALSWVLTVLSLLPLLEAQIPLCANLVPVPITNATLDQITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQDQCIYNTTYLNVQRENGTISRYVGGQEHFAHLLILRDTKTYMLAFDVNDEKNWGLSVYADKPETTKEQLGEFYEALDCLRIPKSDVVYTDWKKDKCEPLEKQHEKERKQEEGES pai1_human Plasminogen activator inhibitor 1 Endothelial plasminogen activator inhibitor SERPINE1 PAI1 PLANH1 Serpin E1 protein protein human Homo sapiens Human MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP agp1-pai1-2 Solid phase binding assay reveals interaction of AGP1 and PAI1proteins 500735 N/A 500739 unspecified role unspecified role neutral component neutral component N/A 500740 unspecified role unspecified role neutral component neutral component P05121:p.[Asn173His;Lys177Thr;Gln342Leu;Met377Ile] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Figure 3 The authors state that a mutant form of P05121 was used in this study. agp1-pai1-3 Solid phase binding assay reveals interaction of AGP1 and PAI1proteins 500736 N/A 500740 unspecified role unspecified role neutral component neutral component P05121:p.[Asn173His;Lys177Thr;Gln342Leu;Met377Ile] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 500739 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 3 The authors state that a mutant form of P05121 was used in this study. agp1-pai1 Colocalisation of AGP1 and PAI1proteins 500737 N/A 500740 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 500739 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Figure 4 A and Figure 5 A AGP preferentially interacted with the active form of PAI1 agp1-pai1-1 Solid phase binding assay reveals interaction of AGP1 and PAI1proteins 500738 N/A 500739 unspecified role unspecified role neutral component neutral component N/A 500740 unspecified role unspecified role neutral component neutral component physical association physical association false false false Figure 4 E AGP preferentially interacted with the active form of PAI1