IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ xie-2006a-1 Identification of the fibroblast growth factor (FGF)-interacting domain in a secreted FGF-binding protein by phage display. in vitro In vitro t7 phage t7 phage display t7 phage nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Xie B., Tassi E., Swift MR., McDonnell K., Bowden ET., Wang S., Ueda Y., Tomita Y., Riegel AT., Wellstein A. BioCreative - Critical Assessment of Information Extraction systems in Biology Biopanning experiment using N-terminal deletion mutants of FGFBP1 wellstea@georgetown.edu J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation xie-2006a-2 Identification of the fibroblast growth factor (FGF)-interacting domain in a secreted FGF-binding protein by phage display. in vitro In vitro competition binding competition binding predetermined predetermined participant predetermined Xie B., Tassi E., Swift MR., McDonnell K., Bowden ET., Wang S., Ueda Y., Tomita Y., Riegel AT., Wellstein A. BioCreative - Critical Assessment of Information Extraction systems in Biology Biopanning experiment using N-terminal deletion mutants of FGFBP1 wellstea@georgetown.edu J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation xie-2006a-3 Identification of the fibroblast growth factor (FGF)-interacting domain in a secreted FGF-binding protein by phage display. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Xie B., Tassi E., Swift MR., McDonnell K., Bowden ET., Wang S., Ueda Y., Tomita Y., Riegel AT., Wellstein A. BioCreative - Critical Assessment of Information Extraction systems in Biology Biopanning experiment using N-terminal deletion mutants of FGFBP1 wellstea@georgetown.edu J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation fgf2_human Fibroblast growth factor 2 Basic fibroblast growth factor FGF2 FGFB Heparin-binding growth factor 2 protein protein human Homo sapiens Human MVGVGGGDVEDVTPRPGGCQISGRGARGCNGIPGAAAWEAALPRRRPRRHPSVNPRSRAAGSPRTRGRRTEERPSGSRLGDRGRGRALPGGRLGGRGRGRAPERVGGRGRGRGTAAPRAAPAARGSRPGPAGTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS fgfp1_human Fibroblast growth factor-binding protein 1 17 kDa heparin-binding growth factor-binding protein FGFBP1 FGFBP HBP17 protein protein human Homo sapiens Human MKICSLTLLSFLLLAAQVLLVEGKKKVKNGLHSKVVSEQKDTLGNTQIKQKSRPGNKGKFVTKDQANCRWAATEQEEGISLKVECTQLDHEFSCVFAGNPTSCLKLKDERVYWKQVARNLRSQKDICRYSKTAVKTRVCRKDFPESSLKLVSSTLFGNTKPRKEKTEMSPREHIKGKETTPSSLAVTQTMATKAPECVEDPDMANQRKTALEFCGETWSSLCTFFLSIVQDTSC fgfbp1-fgf2-1 Interaction between FGFBP1 and FGF2 identified by phage display 501508 N/A 501511 unspecified role unspecified role bait bait N/A 501512 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1 & 2 fgfbp1-fgf2-2 Interaction between FGFBP1 and FGF2 identified by phage display 501509 N/A 501511 unspecified role unspecified role bait bait i125 region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 501512 unspecified role unspecified role prey prey direct interaction direct interaction false false false 3A A fusion protein of MALT and the C-terminal fragment of FGF-BP1 competed for binding. fgfbp1-fgf2-3 Interaction between FGFBP1 and FGF2 identified by SPR 501510 N/A 501511 unspecified role unspecified role bait bait N/A 501512 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 4A & 4B