IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ deval-2006-1 Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr Adult rat dorsal root ganglia cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation deval-2006-2 Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr Adult rat dorsal root ganglia cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation deval-2006-4 Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr Adult rat dorsal root ganglia cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation deval-2006-3 Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. rat-brain Rattus norvegicus brain brain Brain [cerebrum] fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr Adult rat dorsal root ganglia cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation nhrf1_rat Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Nherf1 Ezrin-radixin-moesin-binding phosphoprotein 50 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1 Slc9a3r1 Nherf protein protein rat Rattus norvegicus (Rat) Rat MSADAAAGEPLPRLCCLEKGPNGYGFHLHGEKGKVGQFIRLVEPGSPAEKSGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLKKLGVPIREELLRAQEKSEHTEPPAAADTKKAGDQNEAEKSHLERCELRPRLCTMKKGPNGYGFNLHSDKSKPGQFIRAVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIKAGGDEAKLLVVDKETDEFFKKCRVTPSQEHLDGPLPEPFSNGEIQKENSREALVEPASESPRPALARSASSDTSEELNAQDSPKRHDSTEPSSTSSSSDPILDFNISLAVAKERAHQKRSSKRAPQMDWSKKNELFSNL asic3_rat Acid-sensing ion channel 3 Asic3 Accn3 Amiloride-sensitive cation channel 3 Dorsal root ASIC Drasic protein protein rat Rattus norvegicus (Rat) Rat MKPRSGLEEAQRRQASDIRVFASSCTMHGLGHIFGPGGLTLRRGLWATAVLLSLAAFLYQVAERVRYYGEFHHKTTLDERESHQLTFPAVTLCNINPLRRSRLTPNDLHWAGTALLGLDPAEHAAYLRALGQPPAPPGFMPSPTFDMAQLYARAGHSLEDMLLDCRYRGQPCGPENFTVIFTRMGQCYTFNSGAHGAELLTTPKGGAGNGLEIMLDVQQEEYLPIWKDMEETPFEVGIRVQIHSQDEPPAIDQLGFGAAPGHQTFVSCQQQQLSFLPPPWGDCNTASLDPDDFDPEPSDPLGSPRPRPSPPYSLIGCRLACESRYVARKCGCRMMHMPGNSPVCSPQQYKDCASPALDAMLRKDTCVCPNPCATTRYAKELSMVRIPSRASARYLARKYNRSESYITENVLVLDIFFEALNYEAVEQKAAYEVSELLGDIGGQMGLFIGASLLTILEILDYLCEVFQDRVLGYFWNRRSAQKRSGNTLLQEELNGHRTHVPHLSLGPRPPTTPCAVTKTLSASHRTCYLVTRL nhrf2_rat Na(+)/H(+) exchange regulatory cofactor NHE-RF2 Tyrosine kinase activator protein 1 SRY-interacting protein 1 Solute carrier family 9 isoform A3 regulatory factor 2 NHE3 kinase A regulatory protein E3KARP Sodium-hydrogen exchanger regulatory factor 2 Slc9a3r2 Nherf2 protein protein rat Rattus norvegicus (Rat) Rat MAAPESLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSPAEAAALRAGDRLVEVNGVNVEGETHHQVVHRIKAVEGQTQLLVVDKETDEELCRRQLTCTEEMAHRGLPPAHNPWEPKPDWACSGSLGSDTGHKDVNGPPRELRPRLCHLRRGPQGYGFNLHSDKSRPGQYIRSVDPGSPASLSGLRAQDRLIEVNGQNVEGLRHAEVVARIKAQEDEARLLVVDPETDEHFKRLRVVPTEDHVEGPLPSPVTNGTSLAQLNGGSVCSSRSDLPGSEKDNEDGSAWKRDPFQESGLHLSPTAAEAKEKARATRVNKRAPQMDWNRKREIFSNF ezri_cerae_protein Ezrin (p81) (Cytovillin) (Villin-2) protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt The protein was detected using in situ hybridisation using an anti-ezrin antibody. The source and prooduction details used in production of this assay are not provided in the corresponding paper. asic3-nherf_1 Yeast two hybrid assay reveals interaction between ASIC3 protein and NHERF-1 protein 503589 N/A 503593 unspecified role unspecified role prey prey N/A 503594 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false asic3-nherf_2 Yeast two hybrid assay reveals interaction between ASIC3 protein and NHERF-2 protein 503589 N/A 503595 unspecified role unspecified role prey prey N/A 503594 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false asic3-nherf_1-1 Coimmunoprecipitation assay reveals interaction between ASIC3 protein and NHERF-1 protein 503590 N/A 503593 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region required to bind necessary binding region required to bind range range range range false N/A 503594 unspecified role unspecified role prey prey c terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O35240:p.Ser523Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false O35240:p.Ser523Asp mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The coordiates of the deleted PDZ region for NHERF-1 are not given in the text. The coordinates for the PDZ region were taken from the UniProt entry Q9JJ19. Figure 1A Figure 2 phorbol 12-myristate 13-acetate (PMA) chelerythrine The authors note that "All of these data strongly suggest that PKC phosphorylation of serine 523 in the COOH-terminal region of ASIC3 positively modulates the interaction between ASIC3 and NHERF-1." asic3-nherf_2-1 Coimmunoprecipitation assay reveals interaction between ASIC3 protein and NHERF-2 protein 503590 N/A 503595 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind range range range range false n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 503594 unspecified role unspecified role prey prey c terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false The coordiates of the deleted PDZ region for NHERF-1 are not given in the text. The coordinates for the PDZ region were taken from the UniProt entry Q920G2. Figure 1 B asic3-nherf_1-3 Colocalisation assay reveals interaction between ASIC3 protein and NHERF-1 protein 503591 N/A 503593 unspecified role unspecified role neutral component neutral component n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region required to bind necessary binding region required to bind range range range range false N/A 503594 unspecified role unspecified role neutral component neutral component c terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false Figure 5F asic3-nherf_1-4 Colocalisation assay reveals interaction between ASIC3 protein and NHERF-1 protein 503591 N/A 503593 unspecified role unspecified role neutral component neutral component N/A 503596 unspecified role unspecified role neutral component neutral component N/A 503594 unspecified role unspecified role neutral component neutral component n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false Figure 6 No UniProt accession number is available for Ezrin at this time. asic3-nherf_1-2 Colocalisation assay reveals interaction between ASIC3 protein and NHERF-1 protein 503592 N/A 503593 unspecified role unspecified role neutral component neutral component N/A 503594 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 4E