UniProt UniProt http://www.uniprot.org peng-2011-4 SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org Mol. Cell. Biol. (0270-7306) 2011 mimix curation peng-2011-5 SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org Mol. Cell. Biol. (0270-7306) 2011 mimix curation peng-2011-1 SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. in vitro In vitro acetylase assay acetylase assay predetermined predetermined participant predetermined Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org Mol. Cell. Biol. (0270-7306) 2011 mimix curation peng-2011-2 SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. in vitro In vitro deacetylase assay deacetylase assay predetermined predetermined participant predetermined Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org Mol. Cell. Biol. (0270-7306) 2011 mimix curation peng-2011-3 SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 imaging technique imaging technique microscopy immunostaining immunostaining Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org Mol. Cell. Biol. (0270-7306) 2011 mimix curation dnmt1_human DNA (cytosine-5)-methyltransferase 1 CXXC9 MCMT DNA methyltransferase HsaI CXXC-type zinc finger protein 9 DNMT1 AIM DNMT protein protein human Homo sapiens Human MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD sir1_human NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 SIRT1 SIR2L1 SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein human Homo sapiens Human MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS kat2b_human Histone acetyltransferase KAT2B P300/CBP-associated factor PCAF Lysine acetyltransferase 2B Histone acetyltransferase PCAF KAT2B Spermidine acetyltransferase KAT2B protein protein human Homo sapiens Human MSEAGGAGPGGCGAGAGAGAGPGALPPQPAALPPAPPQGSPCAAAAGGSGACGPATAVAAAGTAEGPGGGGSARIAVKKAQLRSAPRAKKLEKLGVYSACKAEESCKCNGWKNPNPSPTPPRADLQQIIVSLTESCRSCSHALAAHVSHLENVSEEEMNRLLGIVLDVEYLFTCVHKEEDADTKQVYFYLFKLLRKSILQRGKPVVEGSLEKKPPFEKPSIEQGVNNFVQYKFSHLPAKERQTIVELAKMFLNRINYWHLEAPSQRRLRSPNDDISGYKENYTRWLCYCNVPQFCDSLPRYETTQVFGRTLLRSVFTVMRRQLLEQARQEKDKLPLEKRTLILTHFPKFLSMLEEEVYSQNSPIWDQDFLSASSRTSQLGIQTVINPPPVAGTISYNSTSSSLEQPNAGSSSPACKASSGLEANPGEKRKMTDSHVLEEAKKPRVMGDIPMELINEVMSTITDPAAMLGPETNFLSAHSARDEAARLEERRGVIEFHVVGNSLNQKPNKKILMWLVGLQNVFSHQLPRMPKEYITRLVFDPKHKTLALIKDGRVIGGICFRMFPSQGFTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHDILNFLTYADEYAIGYFKKQGFSKEIKIPKTKYVGYIKDYEGATLMGCELNPRIPYTEFSVIIKKQKEIIKKLIERKQAQIRKVYPGLSCFKDGVRQIPIESIPGIRETGWKPSGKEKSKEPRDPDQLYSTLKSILQQVKSHQSAWPFMEPVKRTEAPGYYEVIRFPMDLKTMSERLKNRYYVSKKLFMADLQRVFTNCKEYNPPESEYYKCANILEKFFFSKIKEAGLIDK dnmt1-sirt1-2 1190202 n/a 1190207 unspecified role unspecified role bait bait n/a 1190208 unspecified role unspecified role prey prey physical association physical association false false false 3b, c and d sirt1-dnmt1 1190202 n/a 1190207 unspecified role unspecified role prey prey n/a 1190208 unspecified role unspecified role bait bait physical association physical association false false false 3b dnmt1-sirt1-3 1190203 n/a 1190208 unspecified role unspecified role prey prey n/a 1190207 unspecified role unspecified role bait bait physical association physical association false false false 3b sirt1-dnmt1-1 1190203 n/a 1190207 unspecified role unspecified role prey prey n/a 1190208 unspecified role unspecified role bait bait physical association physical association false false false 3b dnmt1-kat2b 1190204 n/a 1190207 enzyme target enzyme target substrate substrate neutral component neutral component n/a 1190209 enzyme enzyme neutral component neutral component direct interaction direct interaction false false false 1 dnmt1-sirt1 1190205 n/a 1190207 enzyme target enzyme target substrate substrate neutral component neutral component n/a 1190208 enzyme enzyme neutral component neutral component direct interaction direct interaction false false false 2 dnmt1-sirt1-1 1190206 n/a 1190207 unspecified role unspecified role neutral component neutral component n/a 1190208 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3a