IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tan-2011-1 Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tan J.J., Ong S.A., Chen K.S. Apoptosis - Interactions involving proteins with a function related to apoptosis kschen@ntu.edu.sg Mouse brain cDNA BMC Mol. Biol. (null) 2011 Only protein-protein interactions imex curation tan-2011-2 Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip antibody detection identification by antibody antibody detection Tan J.J., Ong S.A., Chen K.S. Apoptosis - Interactions involving proteins with a function related to apoptosis kschen@ntu.edu.sg Mouse brain cDNA BMC Mol. Biol. (null) 2011 Only protein-protein interactions imex curation tan-2011-3 Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Tan J.J., Ong S.A., Chen K.S. Apoptosis - Interactions involving proteins with a function related to apoptosis kschen@ntu.edu.sg Mouse brain cDNA BMC Mol. Biol. (null) 2011 Only protein-protein interactions imex curation tan-2011-4 Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Tan J.J., Ong S.A., Chen K.S. Apoptosis - Interactions involving proteins with a function related to apoptosis kschen@ntu.edu.sg Mouse brain cDNA BMC Mol. Biol. (null) 2011 Only protein-protein interactions imex curation tan-2011-5 Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Tan J.J., Ong S.A., Chen K.S. Apoptosis - Interactions involving proteins with a function related to apoptosis kschen@ntu.edu.sg Mouse brain cDNA BMC Mol. Biol. (null) 2011 Only protein-protein interactions imex curation tan-2011-6 Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Tan J.J., Ong S.A., Chen K.S. Apoptosis - Interactions involving proteins with a function related to apoptosis kschen@ntu.edu.sg Mouse brain cDNA BMC Mol. Biol. (null) 2011 Only protein-protein interactions imex curation nr2f6_mouse Nuclear receptor subfamily 2 group F member 6 Nr2f6 Ear-2 Ear2 Erbal2 COUP transcription factor 3 V-erbA-related protein 2 protein protein mouse Mus musculus Mouse MAMVTGGWGDPGGDTNGVDKAGGSYPRATEDDSASPPGATSDAEPGDEERPGLQVDCVVCGDKSSGKHYGVFTCEGCKSFFKRSIRRNLSYTCRSNRDCQIDQHHRNQCQYCRLKKCFRVGMRKEAVQRGRIPHALPGPAACSPPGATGVEPFTGPPVSELIAQLLRAEPYPAAGRFGGGGAVLGIDNVCELAARLLFSTVEWARHAPFFPELPAADQVALLRLSWSELFVLNAAQAALPLHTAPLLAAAGLHAAPMAAERAVAFMDQVRAFQEQVDKLGRLQVDAAEYGCLKAIALFTPDACGLSDPAHVESLQEKAQVALTEYVRAQYPSQPQRFGRLLLRLPALRAVPASLISQLFFMRLVGKTPIETLIRDMLLSGSTFNWPYGSG rasd1_mouse Dexamethasone-induced Ras-related protein 1 Rasd1 Dexras1 protein protein mouse Mus musculus Mouse MKLAAMIKKMCPSDSELSIPAKNCYRMVILGSSKVGKTAIVSRFLTGRFEDAYTPTIEDFHRKFYSIRGEVYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRDSFEEVQRLKQQILDTKSCLKNKTKENVDVPLVICGNKGDRDFYREVEQREIEQLVGDDPQRCAYFEISAKKNSSLDQMFRALFAMAKLPSEMSPDLHRKVSVQYCDVLHKKALRNKKLLRAGSGGGGDHGDAFGILAPFARRPSVHSDLMYIREKTSVGSQAKDKERCVIS cenpb_mouse Major centromere autoantigen B Cenpb Cenp-b Centromere protein B protein protein mouse Mus musculus Mouse MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVACSGVTRSRARSSAPRAPAAPAGPATVPSEGSGGSTPGWHTREEQPPSVAEGYASQDVFSATETSLWYDFLSDQASGLWGGDGPARQATQRLSVLLCANADGSEKLPPLVAGKSAKPRAGQGGLPCDYTANSKGGVTTQALAKYLKALDTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQAMLLKAMAALEGQDPSGLQLGLVEALHFVAAAWQAVEPSDIATCFREAGFGGGLNATITTSFKSEGEEEEEEEEEEEEEEEEEGEGEEEEEEEEEGEEEGGEGEEEGEEEVEEEGEVDDSDEEEEESSSEGLEAEDWAQGVVEASGGFGGYSVQEEAQFPTLHFLEGGEDSDSDSDEEEDDEEEDEEDEDEEDDEDGDEVPVPSFGEAMAYFAMVKRYLTSFPIDDRVQSHILHLEHDLVHVTRKNHARQAGVRGLGHQS gbb1_mouse Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Gnb1 Transducin beta chain 1 protein protein mouse Mus musculus Mouse MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN pls1_mouse Phospholipid scramblase 1 Plscr1 Tra1b Tras1 Ca(2+)-dependent phospholipid scramblase 1 Transplantability-associated protein 1 Mg(2+)-dependent nuclease protein protein mouse Mus musculus Mouse MENHSKQTEAPHPGTYMPAGYPPPYPPAAFQGPSDHAAYPIPQAGYQGPPGPYPGPQPGYPVPPGGYAGGGPSGFPVQNQPAYNHPGGPGGTPWMPAPPPPLNCPPGLEYLAQIDQLLVHQQIELLEVLTGFETNNKYEIKNSLGQRVYFAVEDTDCCTRNCCGASRPFTLRILDNLGREVMTLERPLRCSSCCFPCCLQEIEIQAPPGVPVGYVTQTWHPCLPKFTLQNEKKQDVLKVVGPCVVCSCCSDIDFELKSLDEESVVGKISKQWSGFVREAFTDADNFGIQFPLDLDVKMKAVMLGACFLIDFMFFERTGNEEQRSGAWQ sh3g2_mouse Endophilin-A1 Sh3gl2 Sh3d2a Endophilin-1 SH3 domain-containing GRB2-like protein 2 SH3 domain protein 2A Een1 SH3p4 protein protein mouse Mus musculus Mouse MSVAGLKKQFHKATQKVSEKVGGAEGTKLDDDFKEMERKVDVTSRAVMEIMTKTIEYLQPNPASRAKLSMINTMSKIRGQEKGPGYPQAEALLAEAMLKFGRELGDDCNFGPALGEVGEAMRELSEVKDSLDMEVKQNFIDPLQNLHDKDLREIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFDESKEIAESSMFNLLEMDIEQVSQLSALVQAQLEYHKQAVQILQQVTVRLEERIRQASSQPRREYQPKPRMSLEFATGDSTQPNGGLSHTGTPKPPGVQMDQPCCRALYDFEPENEGELGFKEGDIITLTNQIDENWYEGMLHGQSGFFPINYVEILVALPH sp16h_mouse FACT complex subunit SPT16 Supt16h Fact140 Factp140 Facilitates chromatin transcription complex subunit SPT16 FACT 140 kDa subunit FACTp140 Chromatin-specific transcription elongation factor 140 kDa subunit Supt16 protein protein mouse Mus musculus Mouse MAVTLDKDAYYRRVKRLYSNWRKGEDEYASIDAIVVSVGVDEEIVYAKSTALQTWLFGYELTDTIMVFCDDKIIFMASKKKVEFLKQIANTKGNENANGAPAITLLVREKNESNKSSFDKMIDAIKESKSGKKIGVFSKDKFPGEFMKSWSDCLNKEGFDKVDISAVVAYTIAVKEDGELNLMKKAASITSEVFNKFFKERVMEIVDADEKVRHSKLAESVEKAIEEKKYLAGADPSTVEMCYPPIIQSGGNYNLKFSVVSDKNHMHFGAITCAMGIRFKSYCSNLVRTLMVDPTQEVQENYNFLLQLQEELLKELRHGVKICDVYNSVMDVVKKQKPELLNKITKNLGFGMGIEFREGSLVINSKNQYKLKKGMVFSINLGFSDLTNKEGKKPEEKTYALFIGDTVLVDEDGPATILTSVKKKVKNVGIFLKNEDDEEEEEEKDEAEDLLGRGSRAALLTERTRNEMTAEEKRRAHQKELAAQLNEEAKRRLTEQKGEQQIQKARKSNVSYKNPSLMPKEPHIREMKIYIDKKYETVIMPVFGIATPFHIATIKNISMSVEGDYTYLRINFYCPGSALGRNEGNIFPNPEATFVKEITYRASNMKAPGEQTVPALNLQNAFRIIKEVQKRYKTREAEEKEKEGIVKQDSLVINLNRSNPKLKDLYIRPNIAQKRMQGSLEAHVNGFRFTSVRGDKVDILYNNIKHALFQPCDGEMIIVLHFHLKNAVMFGKKRHTDVQFYTEVGEITTDLGKHQHMHDRDDLYAEQMEREMRHKLKTAFKNFIEKVEALTKEELEFEVPFRDLGFNGAPYRSTCLLQPTSSALVNATEWPPFVVTLDEVELIHFERVQFHLKNFDMVIVYKDYSKKVTMINAIPVASLDPIKEWLNSCDLKYTEGVQSLNWTKIMKTIVDDPEGFFEQGGWSFLEPEGEGSDAEDGDSESEIEDETFNPSEDDYEEEEEDSDEDYSSEAEESDYSKESLGSEEESGKDWDELEEEARKADRESRYEEEEEQSRSMSRKRKASVHSSGRGSNRGSRHSSAPPKKKRK aspp2_mouse Apoptosis-stimulating of p53 protein 2 Tumor suppressor p53-binding protein 2 Tp53bp2 Aspp2 Trp53bp2 protein protein mouse Mus musculus Mouse MMPMFLTVYLSNSEQHFTEVPVTPETICRDVVDLCKEPGENDCHLAEVWCGSERPVADNERMFDVLQRFGSQRNEVRFFLRHERPPNRDIVSGPRSQDPSVKRNGVKVPGEHRRKENGVNSPRLDLTLAELQEMASRQQQQIEAQQQMLATKEQRLKFLKQQDQRQQQQAAEQEKLKRLREIAESQEAKLKKVRALKGHVEQKRLSNGKLVEEIEQMNSLFQQKQRELVLAVSKVEELTRQLEMLKNGRIDGHHDNQSAVAELDRLYKELQLRNKLNQEQNAKLQQQRECLNKRNSEVAVMDKRVSELRDRLWKKKAALQQKENLPVSPDGNLPQQAVSAPSRVAAVGPYIQSSTMPRMPSRPELLVKPALPDGSLLMQSAEGPMKIQTLPNMRSGAASQSKGSKAHPASPDWNPSNADLLPSQGSSVPQSAGTALDQVDDGEIAVREKEKKVRPFSMFDTVDQCAAPPSFGTLRKNQSSEDILRDAQAVNKNVAKVPPPVPTKPKQIHLPYFGQTAQSPSDMKPDGNAQQLPIAATSVGAKLKPAGPQARMLLSPGAPSGGQDQVLSPASKQESPPAAAVRPFTPQPSKDTFPPAFRKPQTVAASSIYSMYTQQQAPGKNFQQAVQSALTKTQPRGPHFSSVYGKPVIAAAQNPQQHPENIYSCSQGKPGSPEPETETVSSVHESHENERIPRPLSPTKLLPFLSNPYRNQSDADLEALRKKLSNAPRPLKKRSSITEPEGPNGPNIQKLLYQRTTIAAMETISVPSHPSKSPGSVTVNPESSVEIPNPYLHVEPEKEVGSLVPEPLSPEDMGSASTENSDVPAPSAGLEYVSEGVTDSSTNLQNNVEETNPEAPHLLEVYLEEYPPYPPPPYPSGEPEVSEEDSARMRPPEITGQVSLPPGKRTNLRKTGSERIAHGMRVKFNPLALLLDSSLEGEFDLVQRIIYEVDDPSLPNDEGITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFAMTYSDMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQHDDELLMKEGDCMTVIRREDEEEIEWWWARLNDKEGYVPRNLLGLYPRIKPRQRSLA 1433f_mouse 14-3-3 protein eta Ywhah protein protein mouse Mus musculus Mouse MGDREQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSIEQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLALLDKFLIKNCNDFQYESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEHMQPTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDEEAGEGN rasd1_human Dexamethasone-induced Ras-related protein 1 Activator of G-protein signaling 1 RASD1 AGS1 DEXRAS1 protein protein human Homo sapiens Human MKLAAMIKKMCPSDSELSIPAKNCYRMVILGSSKVGKTAIVSRFLTGRFEDAYTPTIEDFHRKFYSIRGEVYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRDSFEEVQRLRQQILDTKSCLKNKTKENVDVPLVICGNKGDRDFYREVDQREIEQLVGDDPQRCAYFEISAKKNSSLDQMFRALFAMAKLPSEMSPDLHRKVSVQYCDVLHKKALRNKKLLRAGSGGGGGDPGDAFGIVAPFARRPSVHSDLMYIREKASAGSQAKDKERCVIS nr2f6_human Nuclear receptor subfamily 2 group F member 6 EAR2 ERBAL2 V-erbA-related protein 2 NR2F6 protein protein human Homo sapiens Human MAMVTGGWGGPGGDTNGVDKAGGYPRAAEDDSASPPGAASDAEPGDEERPGLQVDCVVCGDKSSGKHYGVFTCEGCKSFFKRSIRRNLSYTCRSNRDCQIDQHHRNQCQYCRLKKCFRVGMRKEAVQRGRIPHSLPGAVAASSGSPPGSALAAVASGGDLFPGQPVSELIAQLLRAEPYPAAAGRFGAGGGAAGAVLGIDNVCELAARLLFSTVEWARHAPFFPELPVADQVALLRLSWSELFVLNAAQAALPLHTAPLLAAAGLHAAPMAAERAVAFMDQVRAFQEQVDKLGRLQVDSAEYGCLKAIALFTPDACGLSDPAHVESLQEKAQVALTEYVRAQYPSQPQRFGRLLLRLPALRAVPASLISQLFFMRLVGKTPIETLIRDMLLSGSTFNWPYGSGQ rasd1-nr2f6 1172309 n/a 1172315 unspecified role unspecified role prey prey n/a 1172316 unspecified role unspecified role bait bait physical association physical association false false false Table 1 rasd1-cenpb 1172309 n/a 1172317 unspecified role unspecified role prey prey n/a 1172316 unspecified role unspecified role bait bait physical association physical association false false false Table 1 rasd1-gnb1 1172309 n/a 1172316 unspecified role unspecified role bait bait n/a 1172318 unspecified role unspecified role prey prey physical association physical association false false false Table 1 rasd1-plscr1 1172309 n/a 1172316 unspecified role unspecified role bait bait n/a 1172319 unspecified role unspecified role prey prey physical association physical association false false false Fig. 7B rasd1-sh3gl2 1172309 n/a 1172320 unspecified role unspecified role prey prey n/a 1172316 unspecified role unspecified role bait bait physical association physical association false false false Table 1 rasd1-supt16h 1172309 n/a 1172316 unspecified role unspecified role bait bait n/a 1172321 unspecified role unspecified role prey prey physical association physical association false false false Table 1 rasd1-tp53bp2 1172309 n/a 1172316 unspecified role unspecified role bait bait n/a 1172322 unspecified role unspecified role prey prey physical association physical association false false false Table 1 rasd1-ywhah 1172309 n/a 1172323 unspecified role unspecified role prey prey n/a 1172316 unspecified role unspecified role bait bait physical association physical association false false false Table 1 nr2f6-rasd1-1 1172310 n/a 1172315 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1172316 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 1C nr2f6-rasd1-2 1172311 n/a 1172324 unspecified role unspecified role prey prey n/a 1172325 unspecified role unspecified role bait bait physical association physical association false false false Figure 1E nr2f6-rasd1-4 1172312 n/a 1172315 unspecified role unspecified role bait bait n/a 1172316 unspecified role unspecified role prey prey physical association physical association false false false Figure 1E rasd1-nr2f6-1 1172313 n/a 1172315 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 1172316 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O35626:p.Lys34Asn mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O35626:p.Gly81Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false Figure 1B and 4B nr2f6-rasd1-6 1172314 n/a 1172316 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1172315 unspecified role unspecified role neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Figure 5