IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ watanabe-2011-3 Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. in vitro In vitro protease assay protease assay western blot western blot Immuno blot Immuno blot Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation watanabe-2011-1 Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. in vitro In vitro protease assay protease assay weight by comassie molecular weight estimation by coomasie staining weight by comassie Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation watanabe-2011-2 Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. in vitro In vitro protease assay protease assay anti tag western anti tag western blot anti tag western Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation watanabe-2011-4 Calcium-dependent activation and autolysis of Arabidopsis metacaspase 2d. in vitro In vitro protease assay protease assay tag visualisation tag visualisation Watanabe N., Lam E. Apoptosis - Interactions involving proteins with a function related to apoptosis lam@aesop.rutgers.edu J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation mca4_arath Metacaspase-4 AMC4 AMC7 MCP2D Metacaspase 2d Metacaspase-7 YUP8H12R.4 At1g79340 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTKKAVLIGINYPGTKAELRGCVNDVRRMYKCLVERYGFSEENITVLIDTDESSTQPTGKNIRRALADLVESADSGDVLVVHYSGHGTRLPAETGEDDDTGFDECIVPCDMNLITDDDFRDLVDKVPPGCRMTIISDSCHSGGLIDEAKEQIGESTKKEAEDEDESEESSSRFGFRKFLRSKVEGAIESRGFHIGGNKKDEDEAEEIETKEIELEDGETIHAKDKSLPLQTLIDILKQQTGNDNIEVGKIRPSLFDAFGDDSSPKVKKFMKVILGKLQAGNGEEGGLMGMLGKLASGFLEGKLNDEDYVKPAMQTHVGSKEEVYAGGSRGSVPLPDSGILISGCQTDQTSADATPAGKPTEAYGAMSNSIQTILEETDGEISNREMVTRARKALKKQGFTQQPGLYCHDGYANAPFIC amc4-2 1280616 n/a 1280620 enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1280620 enzyme enzyme neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1280620 enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false O64517:p.Cys139Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false t7 tag t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 4a (left) Ca2+ is required for the amc4 protease to show this autocatalytic activity. amc4-amc4 1280616 n/a 1280620 putative self putative self neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) putative self interaction putative self interaction false false false 5b, 6a, 6b, S5b and S6 Ca2+ is required for the amc4 protease to show this autocatalytic activity. amc4 1280617 n/a 1280620 putative self putative self neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O64517:p.Cys139Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O64517:p.Lys225Gly mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) putative self interaction putative self interaction false false false 2a, 2b, 2e and S2a Ca2+ is required for the amc4 protease to show this autocatalytic activity. amc4-1 1280618 n/a 1280620 enzyme enzyme neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1280620 enzyme target enzyme target substrate substrate neutral component neutral component purified purified t7 tag t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false O64517:p.Cys139Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 4a (right) Ca2+ is required for the amc4 protease to show this autocatalytic activity. amc4-1 1280619 n/a 1280620 putative self putative self putative self putative self purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false putative self interaction putative self interaction false true false 5b, 6a, 6b and S6 (right) Ca2+ is required for the amc4 protease to show this autocatalytic activity. The cleavage is inhibited by FPR-cmk and FK-tmbk.