I2D I2D xiong-2011-3 Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. sunyi@umich.edu Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer xiong-2011-4 Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. sunyi@umich.edu Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer xiong-2011-5 Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. sunyi@umich.edu Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer xiong-2011-1 Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. sunyi@umich.edu Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer xiong-2011-2 Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. sunyi@umich.edu Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer xiong-2011-6 Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Xiong X., Zhao Y., He H., Sun Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that all constructs are of human origin. sunyi@umich.edu Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer rs27l_human 40S ribosomal protein S27-like RPS27L Small ribosomal subunit protein eS27-like protein protein human Homo sapiens Human MPLARDLLHPSLEEEKKKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP rs27_human 40S ribosomal protein S27 RPS27 MPS1 Metallopan-stimulin 1 Small ribosomal subunit protein eS27 protein protein human Homo sapiens Human MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD rs27l_mouse 40S ribosomal protein S27-like Rps27l protein protein mouse Mus musculus Mouse MPLARDLLHPSLEEEKKKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH rps27l-mdm2 1142245 n/a 1142251 unspecified role unspecified role bait bait n/a 1142252 unspecified role unspecified role prey prey physical association physical association false false false 4a rps27-mdm2 1142245 n/a 1142252 unspecified role unspecified role prey prey n/a 1142253 unspecified role unspecified role bait bait physical association physical association false false false 4a mdm2-rps27-1 1142245 n/a 1142253 unspecified role unspecified role prey prey n/a 1142252 unspecified role unspecified role bait bait physical association physical association false false false 4b mdm2-rps27l-1 1142245 n/a 1142251 unspecified role unspecified role prey prey n/a 1142252 unspecified role unspecified role bait bait physical association physical association false false false 4b mdm2-rps27l-2 1142246 n/a 1142251 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1142252 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q00987:p.[Lys446Arg;Cys475Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4a mdm2-rps27-4 1142246 n/a 1142253 unspecified role unspecified role unspecified role unspecified role n/a 1142252 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q00987:p.[Lys446Arg;Cys475Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4a mdm2-tp53-5 1142246 n/a 1142252 unspecified role unspecified role bait bait Q00987:p.[Lys446Arg;Cys475Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1142254 unspecified role unspecified role prey prey physical association physical association false false false 4c mdm2-tp53-6 1142247 n/a 1142254 unspecified role unspecified role prey prey n/a 1142252 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q00987:p.[Lys446Arg;Cys475Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4d mdm2-rps27-3 1142247 n/a 1142252 unspecified role unspecified role bait bait n/a 1142253 unspecified role unspecified role prey prey physical association physical association false false false 4d mdm2-rps27l-3 1142247 n/a 1142252 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q00987:p.[Lys446Arg;Cys475Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1142251 unspecified role unspecified role prey prey physical association physical association false false false 4d mdm2-rps27-1 1142248 n/a 1142253 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1142252 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 3a mdm2-rps27l 1142248 n/a 1142252 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1142251 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 3a mdm2-rs27l 1142249 n/a 1142252 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1142255 unspecified role unspecified role prey prey mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. physical association physical association false false false 3c Cell lysates were from p53/Mdm2 double-null MEF cells. mdm2-rps27l-4 1142250 n/a 1142252 unspecified role unspecified role neutral component neutral component n/a 1142251 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 8a