IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ duce-2010-1 Iron-Export Ferroxidase Activity of ?-Amyloid Precursor Protein Is Inhibited by Zinc in Alzheimer's Disease. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Duce JA., Tsatsanis A., Cater MA., James SA., Robb E., Wikhe K., Leong SL., Perez K., Johanssen T., Greenough MA., Cho HH., Galatis D., Moir RD., Masters CL., McLean C., Tanzi RE., Cappai R., Barnham KJ., Ciccotosto GD., Rogers JT., Bush AI. Apoptosis - Interactions involving proteins with a function related to apoptosis Conversion of Fe2+ to Fe3+ was measured by monitoring the spectroscopic change in apo-transferrin when loaded with Fe3+. abush@mhri.edu.au Cell (0092-8674) 2010 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease duce-2010-4 Iron-Export Ferroxidase Activity of ?-Amyloid Precursor Protein Is Inhibited by Zinc in Alzheimer's Disease. human-brain Homo sapiens brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Duce JA., Tsatsanis A., Cater MA., James SA., Robb E., Wikhe K., Leong SL., Perez K., Johanssen T., Greenough MA., Cho HH., Galatis D., Moir RD., Masters CL., McLean C., Tanzi RE., Cappai R., Barnham KJ., Ciccotosto GD., Rogers JT., Bush AI. Apoptosis - Interactions involving proteins with a function related to apoptosis Conversion of Fe2+ to Fe3+ was measured by monitoring the spectroscopic change in apo-transferrin when loaded with Fe3+. abush@mhri.edu.au Cell (0092-8674) 2010 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease duce-2010-5 Iron-Export Ferroxidase Activity of ?-Amyloid Precursor Protein Is Inhibited by Zinc in Alzheimer's Disease. mouse-brain-cortex Mus musculus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Duce JA., Tsatsanis A., Cater MA., James SA., Robb E., Wikhe K., Leong SL., Perez K., Johanssen T., Greenough MA., Cho HH., Galatis D., Moir RD., Masters CL., McLean C., Tanzi RE., Cappai R., Barnham KJ., Ciccotosto GD., Rogers JT., Bush AI. Apoptosis - Interactions involving proteins with a function related to apoptosis Conversion of Fe2+ to Fe3+ was measured by monitoring the spectroscopic change in apo-transferrin when loaded with Fe3+. abush@mhri.edu.au Cell (0092-8674) 2010 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease duce-2010-2 Iron-Export Ferroxidase Activity of ?-Amyloid Precursor Protein Is Inhibited by Zinc in Alzheimer's Disease. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Duce JA., Tsatsanis A., Cater MA., James SA., Robb E., Wikhe K., Leong SL., Perez K., Johanssen T., Greenough MA., Cho HH., Galatis D., Moir RD., Masters CL., McLean C., Tanzi RE., Cappai R., Barnham KJ., Ciccotosto GD., Rogers JT., Bush AI. Apoptosis - Interactions involving proteins with a function related to apoptosis Conversion of Fe2+ to Fe3+ was measured by monitoring the spectroscopic change in apo-transferrin when loaded with Fe3+. abush@mhri.edu.au Cell (0092-8674) 2010 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease duce-2010-3 Iron-Export Ferroxidase Activity of ?-Amyloid Precursor Protein Is Inhibited by Zinc in Alzheimer's Disease. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip tag visualisation tag visualisation Duce JA., Tsatsanis A., Cater MA., James SA., Robb E., Wikhe K., Leong SL., Perez K., Johanssen T., Greenough MA., Cho HH., Galatis D., Moir RD., Masters CL., McLean C., Tanzi RE., Cappai R., Barnham KJ., Ciccotosto GD., Rogers JT., Bush AI. Apoptosis - Interactions involving proteins with a function related to apoptosis Conversion of Fe2+ to Fe3+ was measured by monitoring the spectroscopic change in apo-transferrin when loaded with Fe3+. abush@mhri.edu.au Cell (0092-8674) 2010 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease iron2plus iron(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p05067-4 Amyloid-beta precursor protein APP A4 AD1 ABPP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II APPI PreA4 695 Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVRVPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN A major isoform. ceru_human Ceruloplasmin CP Ferroxidase protein protein human Homo sapiens Human MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPDRIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHSHGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIYHSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTYCSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVHAAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFFQVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNADPVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENESLLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYLFSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDHYTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQNVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDKVKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAYYSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVFDIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETTYTVLQNEDTKSG a4_human Amyloid-beta precursor protein APP A4 AD1 Alzheimer disease amyloid protein ABPP PreA4 Cerebral vascular amyloid peptide Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN s40a1_human Solute carrier family 40 member 1 Ferroportin-1 Iron-regulated transporter 1 SLC40A1 FPN1 IREG1 SLC11A3 MSTP079 protein protein human Homo sapiens Human MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLLTAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHELLTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRIDQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLKEEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWVSYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAFTWLRRKCGLVRTGLISGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITPTKIPEITTEIYMSNGSNSANIVPETSPESVPIISVSLLFAGVIAARIGLWSFDLTVTQLLQENVIESERGIINGVQNSMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHIMYFRFAQNTLGNKLFACGPDAKEVRKENQANTSVV s40a1_mouse Solute carrier family 40 member 1 Slc40a1 Fpn1 Ireg1 Slc11a3 Slc39a1 Ferroportin-1 Iron-regulated transporter 1 Metal transporter protein 1 protein protein mouse Mus musculus Mouse MTKARDQTHQEGCCGSLANYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLLTAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKNELLTMYHGWVLTVCYILIITIANIANLASTATAITIQRDWIVVVAGENRSRLADMNATIRRIDQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYFLLWKVYQKTPALAVKAALKVEESELKQLTSPKDTEPKPLEGTHLMGEKDSNIRELECEQEPTCASQMAEPFRTFRDGWVSYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAFTWLRRKCGLVRTGLFSGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFVNVEPVSPTTKIPETVFTTEMHMSNMSNVHEMSTKPIPIVSVSLLFAGVIAARIGLWSFDLTVTQLLQENVIESERGIINGVQNSMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHLMYFRFAQKTLGNQIFVCGPDEKEVTDENQPNTSVV a4_mouse Amyloid-beta precursor protein App Alzheimer disease amyloid A4 protein homolog ABPP Amyloidogenic glycoprotein Amyloid precursor protein A4 AD1 Alzheimer disease amyloid protein Amyloid-beta (A4) precursor protein Amyloid-beta A4 protein protein protein mouse Mus musculus Mouse MLPSLALLLLAAWTVRALEVPTDGNAGLLAEPQIAMFCGKLNMHMNVQNGKWESDPSGTKTCIGTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHTHIVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDSVDSADAEEDDSDVWWGGADTDYADGGEDKVVEVAEEEEVADVEEEEADDDEDVEDGDEVEEEAEEPYEEATERTTSTATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVCGSVSTQSLLKTTSEPLPQDPDKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHPFGVDSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN ceru_mouse Ceruloplasmin Cp Ferroxidase protein protein mouse Mus musculus Mouse MKFLLLSTFIFLYSSLALARDKHYFIGITEAVWDYASGTEEKKLISVDTEQSNFYLQNGPDRIGRKYKKALYFEYTDGTFSKTIDKPAWLGFLGPVIKAEVEDKVYVHLKNLASRIYTFHAHGVTYTKEYEGAVYPDNTTDFQRADDKVLPGQQYVYVLHANEPSPGEGDSNCVTRIYHSHVDAPKDIASGLIGPLILCKKGSLYKEKEKNIDQEFVLMFSVVDENLSWYLEDNIKTFCSEPEKVDKDNEDFQESNRMYSINGYTFGSLPGLSMCAADRVKWYLFGMGNEVDVHSAFFHGQALTSRNYQTDIINLFPATLIDAYMVAQNPGVWMLSCQNLNHLKAGLQAFFQVRDCNKPSPEDNIQDRHVRHYYIAAEEVIWNYAPSGTDIFTGENLTALESDSRVFFEQGATRIGGSYKKMAYREYTDGSFTNRKQRGPDEEHLGILGPVIWAEVGDTIKVTFHNKGQHPLSIQPMGVSFTAENEGTYYGPPGRSSQQAASHVAPKETFTYEWTVPKEMGPTYADPVCLSKMYYSGVDPTKDIFTGLIGPMKICKKGSLLADGRQKDVDKEFYLFPTVFDENESLLLDDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLNMCLGESIVWYLFSAGNEADVHGIYFSGNTYLSKGERRDTANLFPHKSLTLLMNPDTKGTFDVECLTTDHYTGGMKQKYTVNQCQRQFEDFTVYLGERTYYVAAVEVEWDYSPSRAWEKELHHLQEQNVSNVFLDKEEFFIGSKYKKVVYRQFTDSSFREQVKRRAEDEHLGILGPPIHANVGDKVKVVFKNMATRPYSIHAHGVKTESSTVVPTLPGEVRTYTWQIPERSGAGREDSACIPWAYYSTVDRVKDLYSGLIGPLIVCRKSYVKVFSPKKKMEFFLLFLVFDENESWYLDDNIKTYSEHPEKVNKDNEEFLESNKMHAINGKMFGNLQGLTMHVKDEVNWYVMGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVFDLFPGTYQTLEMFPQTPGTWLLHCHVTDHVHAGMATTYTVLPVEQETKSG iron2plus-a4 Ferroxidase activity of A4 986644 n/a 986649 enzyme target enzyme target substrate substrate neutral component neutral component n/a 986650 enzyme enzyme neutral component neutral component picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false Vmax = 228.6 uM Fe3+/min/uM APP; Km = 48.6 uM Figs. 1D, 2A, 2E, 6C, Supp. Fig. 1D pH 7.2 Temp=37C Activity was maintained over a pH range 5.0-9.0 zinc A shorter A4 E2 domain construct (290-420) bound Fe2+ with greatly reduced affinity. Ferroxidase activity of the E2 domain of APP is potentiated by the E1 (28-189) domain in a concentration-dependent manner up to a 1:1 stoichiometry. iron2plus-cp-1 Ferroxidase activity of Ceruloplasmin 986644 n/a 986651 enzyme enzyme neutral component neutral component n/a 986649 enzyme target enzyme target substrate substrate neutral component neutral component direct interaction direct interaction false false false Vmax = 53.0 uM Fe3+/min/uM APP; Km = 201.2 uM Fig. 1E pH 7.2 Temp=37C iron2plus-a4-1 Ferroxidase activity of A4 986644 n/a 986650 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P05067-4:p.Glu340Asn Alters kinetics of interaction mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P05067-4:p.Glu339_Glu340delinsAlaMet Alters kinetics of interaction mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 986649 enzyme target enzyme target substrate substrate neutral component neutral component direct interaction direct interaction false false false Vmax = 92.1 uM Fe3+/min/uM APP; Km = 44.4 uM Fig. 2C pH 7.2 Temp=37C a4-slc40a1-3 Interaction between A4 and SLC40A1 (Ferroportin) 986645 n/a 986652 unspecified role unspecified role bait bait n/a 986653 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4D cp-slc40a1-1 Interaction between CP and SLC40A1 (Ferroportin) 986645 n/a 986651 unspecified role unspecified role bait bait n/a 986653 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4D a4-slc40a1-5 Interaction between A4 and SLC40A1 (Ferroportin) 986645 n/a 986653 unspecified role unspecified role bait bait n/a 986652 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4D, Supp. Fig. 4C That membrane-bound full-length APP interacts with ferroportin was confirmed using APP detection antibodies for both the N- and C-terminal ends of the protein. a4-slc40a1-4 Interaction between A4 and SLC40A1 (Ferroportin) 986646 n/a 986654 unspecified role unspecified role prey prey n/a 986655 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4D cp-slc40a1-2 Interaction between CP and SLC40A1 (Ferroportin) 986646 n/a 986656 unspecified role unspecified role bait bait n/a 986654 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4D, Supp. fig. 4D Interaction slightly but significantly increased in APP−/− cells. a4-slc40a1-6 Interaction between A4 and SLC40A1 (Ferroportin) 986646 n/a 986654 unspecified role unspecified role bait bait n/a 986655 unspecified role unspecified role prey prey physical association physical association false false false Supp. Figs. 4A, 4C Interaction did not occur in A4-/- mice. a4-slc40a1-1 Interaction between A4 and SLC40A1 (Ferroportin) 986647 n/a 986653 unspecified role unspecified role prey prey n/a 986652 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3E Iron slc40a1-a4-1 Interaction between A4 and SLC40A1 (Ferroportin) 986647 n/a 986653 unspecified role unspecified role bait bait n/a 986652 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 2G Fe(NH4)2(SO4)2 a4-slc40a1-2 Interaction between A4 adn SLC40A1 (Ferroportin) 986648 n/a 986650 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 986653 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3E