IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ drost-2010-6 BRD7 is a candidate tumour suppressor gene required for p53 function. human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis For BRD7 ChIP, formaldehyde crosslinking was preceded by protein–protein crosslinking with 2 mM disuccinimidyl glutarate. BJ/ET/RasV12ER cells were used:-BJ fibroblasts expressing ecotropic receptor, hTERT and 4-OH-tamoxifen (4-OHT)-inducible oncogenic H-RasV12. p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer drost-2010-7 BRD7 is a candidate tumour suppressor gene required for p53 function. human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis BJ/ET/RasV12ER cells were used:-BJ fibroblasts expressing ecotropic receptor, hTERT and 4-OH-tamoxifen (4-OHT)-inducible oncogenic H-RasV12. p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer drost-2010-3 BRD7 is a candidate tumour suppressor gene required for p53 function. human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis BJ/ET/RasV12ER cells were used:-BJ fibroblasts expressing ecotropic receptor, hTERT and 4-OH-tamoxifen (4-OHT)-inducible oncogenic H-RasV12. p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer drost-2010-4 BRD7 is a candidate tumour suppressor gene required for p53 function. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer drost-2010-5 BRD7 is a candidate tumour suppressor gene required for p53 function. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer drost-2010-1 BRD7 is a candidate tumour suppressor gene required for p53 function. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer drost-2010-2 BRD7 is a candidate tumour suppressor gene required for p53 function. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer drost-2010-8 BRD7 is a candidate tumour suppressor gene required for p53 function. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer drost-2010-9 BRD7 is a candidate tumour suppressor gene required for p53 function. human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. confocal microscopy confocal microscopy immunostaining immunostaining Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis BJ/ET/RasV12ER cells were used:-BJ fibroblasts expressing ecotropic receptor, hTERT and 4-OH-tamoxifen (4-OHT)-inducible oncogenic H-RasV12. p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer drost-2010-10 BRD7 is a candidate tumour suppressor gene required for p53 function. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. pull down pull down western blot western blot Immuno blot Immuno blot Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer cdkn1a_human_gene Human CDKN1A gene gene gene human Homo sapiens Human brd7_human Bromodomain-containing protein 7 75 kDa bromodomain protein Protein CELTIX-1 BRD7 BP75 CELTIX1 protein protein human Homo sapiens Human MGKKHKKHKSDKHLYEEYVEKPLKLVLKVGGNEVTELSTGSSGHDSSLFEDKNDHDKHKDRKRKKRKKGEKQIPGEEKGRKRRRVKEDKKKRDRDRVENEAEKDLQCHAPVRLDLPPEKPLTSSLAKQEEVEQTPLQEALNQLMRQLQRKDPSAFFSFPVTDFIAPGYSMIIKHPMDFSTMKEKIKNNDYQSIEELKDNFKLMCTNAMIYNKPETIYYKAAKKLLHSGMKILSQERIQSLKQSIDFMADLQKTRKQKDGTDTSQSGEDGGCWQREREDSGDAEAHAFKSPSKENKKKDKDMLEDKFKSNNLEREQEQLDRIVKESGGKLTRRLVNSQCEFERRKPDGTTTLGLLHPVDPIVGEPGYCPVRLGMTTGRLQSGVNTLQGFKEDKRNKVTPVLYLNYGPYSSYAPHYDSTFANISKDDSDLIYSTYGEDSDLPSDFSIHEFLATCQDYPYVMADSLLDVLTKGGHSRTLQEMEMSLPEDEGHTRTLDTAKEMEITEVEPPGRLDSSTQDRLIALKAVTNFGVPVEVFDSEEAEIFQKKLDETTRLLRELQEAQNERLSTRPPPNMICLLGPSYREMHLAEQVTNNLKELAQQVTPGDIVSTYGVRKAMGISIPSPVMENNFVDLTEDTEEPKKTDVAECGPGGS mdm2_human_gene Human MDM2 gene gene gene human Homo sapiens Human bbc3_human_gene Human BBC3 gene gene gene human Homo sapiens Human fas_human_gene Human FAS gene gene gene human Homo sapiens Human bax_human_gene Human BAX gene gene gene human Homo sapiens Human ep300_human Histone acetyltransferase p300 Histone butyryltransferase p300 Histone crotonyltransferase p300 Protein propionyltransferase p300 EP300 P300 E1A-associated protein p300 Protein 2-hydroxyisobutyryltransferase p300 Protein lactyltransferas p300 protein protein human Homo sapiens Human MAENVVEPGPPSAKRPKLSSPALSASASDGTDFGSLFDLEHDLPDELINSTELGLTNGGDINQLQTSLGMVQDAASKHKQLSELLRSGSSPNLNMGVGGPGQVMASQAQQSSPGLGLINSMVKSPMTQAGLTSPNMGMGTSGPNQGPTQSTGMMNSPVNQPAMGMNTGMNAGMNPGMLAAGNGQGIMPNQVMNGSIGAGRGRQNMQYPNPGMGSAGNLLTEPLQQGSPQMGGQTGLRGPQPLKMGMMNNPNPYGSPYTQNPGQQIGASGLGLQIQTKTVLSNNLSPFAMDKKAVPGGGMPNMGQQPAPQVQQPGLVTPVAQGMGSGAHTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHCQSGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDKRNQQPILTGAPVGLGNPSSLGVGQQSAPNLSTVSQIDPSSIERAYAALGLPYQVNQMPTQPQVQAKNQQNQQPGQSPQGMRPMSNMSASPMGVNGGVGVQTPSLLSDSMLHSAINSQNPMMSENASVPSLGPMPTAAQPSTTGIRKQWHEDITQDLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANNRAEYYHLLAEKIYKIQKELEEKRRTRLQKQNMLPNAAGMVPVSMNPGPNMGQPQPGMTSNGPLPDPSMIRGSVPNQMMPRITPQSGLNQFGQMSMAQPPIVPRQTPPLQHHGQLAQPGALNPPMGYGPRMQQPSNQGQFLPQTQFPSQGMNVTNIPLAPSSGQAPVSQAQMSSSSCPVNSPIMPPGSQGSHIHCPQLPQPALHQNSPSPVPSRTPTPHHTPPSIGAQQPPATTIPAPVPTPPAMPPGPQSQALHPPPRQTPTPPTTQLPQQVQPSLPAAPSADQPQQQPRSQQSTAASVPTPTAPLLPPQPATPLSQPAVSIEGQVSNPPSTSSTEVNSQAIAEKQPSQEVKMEAKMEVDQPEPADTQPEDISESKVEDCKMESTETEERSTELKTEIKEEEDQPSTSATQSSPAPGQSKKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAGFVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHASDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLSRGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQDRFVYTCNECKHHVETRWHCTVCEDYDLCITCYNTKNHDHKMEKLGLGLDDESNNQQAAATQSPGDSRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPICKQLIALCCYHAKHCQENKCPVPFCLNIKQKLRQQQLQHRLQQAQMLRRRMASMQRTGVVGQQQGLPSPTPATPTTPTGQQPTTPQTPQPTSQPQPTPPNSMPPYLPRTQAAGPVSQGKAAGQVTPPTPPQTAQPPLPGPPPAAVEMAMQIQRAAETQRQMAHVQIFQRPIQHQMPPMTPMAPMGMNPPPMTRGPSGHLEPGMGPTGMQQQPPWSQGGLPQPQQLQSGMPRPAMMSVAQHGQPLNMAPQPGLGQVGISPLKPGTVSQQALQNLLRTLRSPSSPLQQQQVLSILHANPQLLAAFIKQRAAKYANSNPQPIPGQPGMPQGQPGLQPPTMPGQQGVHSNPAMQNMNPMQAGVQRAGLPQQQPQQQLQPPMGGMSPQAQQMNMNHNTMPSQFRDILRRQQMMQQQQQQGAGPGIGPGMANHNQFQQPQGVGYPPQQQQRMQHHMQQMQQGNMGQIGQLPQALGAEAGASLQAYQQRLLQQQMGSPVQPNPMSPQQHMLPNQAQSPHLQGQQIPNSLSNQVRSPQPVPSPRPQSQPPHSSPSPRMQPQPSPHHVSPQTSSPHPGLVAAQANPMEQGHFASPDQNSMLSQLASNPGMANLHGASATDLGLSTDNSDLNSNLSQSTLDIH p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD brd7-cdkn1a-1 1186025 n/a 1186035 unspecified role unspecified role prey prey n/a 1186036 unspecified role unspecified role bait bait association association false false false Fig 5A BRD7 bound at the upstream (−2.3 kilobases (kb)) p53-binding site in the P21 promoter. Addition of Etoposide resulted in an increase in chromatin-bound acetylated p53 on all target promoters examined. brd7-mdm2 1186025 n/a 1186036 unspecified role unspecified role bait bait n/a 1186037 unspecified role unspecified role prey prey association association false false false Fig 5A BRD7 bound at thep53-binding region of the HDM2 promoter. Addition of Etoposide resulted in an increase in chromatin-bound acetylated p53 on all target promoters examined. brd7-bbc3 1186025 n/a 1186038 unspecified role unspecified role prey prey n/a 1186036 unspecified role unspecified role bait bait association association false false false Fig 5A, 6F. Less binding of BRD7 was detected on the promoters of the p53 target genes that do not require BRD7 for their transcription, including PUMA, FAS and BAX, although it was higher than that to the promoter of a non-transcribed gene. Addition of Etoposide resulted in an increase in chromatin-bound acetylated p53 on all target promoters examined. brd7-fas 1186025 n/a 1186036 unspecified role unspecified role bait bait n/a 1186039 unspecified role unspecified role prey prey association association false false false Fig 5A, 6F. Less binding of BRD7 was detected on the promoters of the p53 target genes that do not require BRD7 for their transcription, including PUMA, FAS and BAX, although it was higher than that to the promoter of a non-transcribed gene. Addition of Etoposide resulted in an increase in chromatin-bound acetylated p53 on all target promoters examined. brd7-bax 1186025 n/a 1186036 unspecified role unspecified role bait bait n/a 1186040 unspecified role unspecified role prey prey association association false false false Fig 5A Less binding of BRD7 was detected on the promoters of the p53 target genes that do not require BRD7 for their transcription, including PUMA, FAS and BAX, although it was higher than that to the promoter of a non-transcribed gene. ep300-cdkn1a 1186025 n/a 1186041 unspecified role unspecified role bait bait n/a 1186035 unspecified role unspecified role prey prey association association false false false Fig 6A p300 bound to the p21 promoter. brd7-cdkn1a 1186026 n/a 1186036 unspecified role unspecified role bait bait n/a 1186035 unspecified role unspecified role unspecified role unspecified role association association false false false Fig 5C BRD7 bound at the upstream (−2.3 kilobases (kb)) p53-binding site in the P21 promoter. brd7-mdm2-1 1186026 n/a 1186037 unspecified role unspecified role prey prey n/a 1186036 unspecified role unspecified role bait bait association association false false false Fig 5C BRD7 showed much broader chromatin binding than p53 at the p21 locus, but a similar profile at the HDM2 locus. tp53-mdm2-3 1186026 n/a 1186042 unspecified role unspecified role bait bait n/a 1186037 unspecified role unspecified role prey prey association association false false false Fig 5C BRD7 showed much broader chromatin binding than p53 at the p21 locus, but a similar profile at the HDM2 locus. tp53-cdkn1a-4 1186026 n/a 1186042 unspecified role unspecified role bait bait n/a 1186035 unspecified role unspecified role prey prey association association false false false Fig 5C BRD7 bound at the upstream (−2.3 kilobases (kb)) p53-binding site in the P21 promoter. brd7-tp53 1186027 n/a 1186042 unspecified role unspecified role prey prey n/a 1186036 unspecified role unspecified role bait bait physical association physical association false false false Fig 3A 4-OHT (4-hydroxy tamoxifan) induces expression of RasV12, which increased the interaction between the two proteins. brd7-ep300 1186027 n/a 1186041 unspecified role unspecified role prey prey n/a 1186036 unspecified role unspecified role bait bait physical association physical association false false false Fig 6B brd7-tp53-1 1186028 n/a 1186036 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186042 unspecified role unspecified role prey prey c_terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false Fig 3B. 4-OHT (4-hydroxy tamoxifan) brd7-tp53-6 1186028 n/a 1186042 unspecified role unspecified role prey prey in vitro In vitro n/a 1186036 unspecified role unspecified role bait bait n_terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 3C. 4-OHT (4-hydroxy tamoxifan) brd7-tp53-2 1186029 n/a 1186042 unspecified role unspecified role prey prey n/a 1186036 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 3D BRD7 and BRD7-Delta-BD (a mutant lacking the bromodomain) bound equally well to wild-type p53 and p53-9KR (a mutant in which nine lysines in the carboxy terminus - representing potential acetylation sites, were mutated to arginines). brd7-tp53-5 1186029 n/a 1186041 unspecified role unspecified role prey prey n/a 1186036 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false S11A tp53-brd7 1186030 n/a 1186042 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1186036 unspecified role unspecified role prey prey physical association physical association false false false S5 tp53-brd7-2 1186031 n/a 1186036 unspecified role unspecified role prey prey n/a 1186042 unspecified role unspecified role bait bait c_terminal_region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false S5 tp53-brd7-3 1186032 n/a 1186036 unspecified role unspecified role prey prey n/a 1186042 unspecified role unspecified role bait bait physical association physical association false false false Fig 6C BRD7 and BRD7-Delta-BD (a mutant lacking the bromodomain) bound equally well to wild-type p53 and p53-9KR (a mutant in which nine lysines in the carboxy terminus - representing potential acetylation sites, were mutated to arginines). brd7-tp53-4 1186033 n/a 1186036 unspecified role unspecified role neutral component neutral component n/a 1186042 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S3C brd7-ep300-1 1186034 n/a 1186041 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186036 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false S11B