IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ redondo_mun-2010-1 Matrix metalloproteinase-9 promotes chronic lymphocytic leukemia b cell survival through its hemopexin domain. human-pbl-bcll Homo sapiens pbl-bcll: PBLs from human B cell Chronic Lymphocytic Leukaemia patients peripheral_blood_lymphocyte-bcll pbl-bcll: PBLs from B cell Chronic Lymphocytic Leukaemia patients B cell population was >95% CD19+ and >90% CD5+. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Redondo-Munoz J., Ugarte-Berzal E., Terol MJ., Van den Steen PE., Hernandez del Cerro M., Roderfeld M., Roeb E., Opdenakker G., Garcia-Marco JA., Garcia-Pardo A. Apoptosis - Interactions involving proteins with a function related to apoptosis DNA Pull-down Assay using biotinylated 22 nucleotide probe containing the specific binding site for STAT factors in the Mcl-1 promoter, and Streptavidin-Conjugated Beads. agarciapardo@cib.csic.es Cancer Cell (1535-6108) 2010 Only protein-protein interactions 6 imex curation Cancer - Interactions investigated in the context of cancer redondo_mun-2010-2 Matrix metalloproteinase-9 promotes chronic lymphocytic leukemia b cell survival through its hemopexin domain. human-pbl-bcll Homo sapiens pbl-bcll: PBLs from human B cell Chronic Lymphocytic Leukaemia patients peripheral_blood_lymphocyte-bcll pbl-bcll: PBLs from B cell Chronic Lymphocytic Leukaemia patients B cell population was >95% CD19+ and >90% CD5+. confocal microscopy confocal microscopy immunostaining immunostaining Redondo-Munoz J., Ugarte-Berzal E., Terol MJ., Van den Steen PE., Hernandez del Cerro M., Roderfeld M., Roeb E., Opdenakker G., Garcia-Marco JA., Garcia-Pardo A. Apoptosis - Interactions involving proteins with a function related to apoptosis DNA Pull-down Assay using biotinylated 22 nucleotide probe containing the specific binding site for STAT factors in the Mcl-1 promoter, and Streptavidin-Conjugated Beads. agarciapardo@cib.csic.es Cancer Cell (1535-6108) 2010 Only protein-protein interactions 6 imex curation Cancer - Interactions investigated in the context of cancer redondo_mun-2010-3 Matrix metalloproteinase-9 promotes chronic lymphocytic leukemia b cell survival through its hemopexin domain. human-pbl-bcll Homo sapiens pbl-bcll: PBLs from human B cell Chronic Lymphocytic Leukaemia patients peripheral_blood_lymphocyte-bcll pbl-bcll: PBLs from B cell Chronic Lymphocytic Leukaemia patients B cell population was >95% CD19+ and >90% CD5+. pull down pull down western blot western blot Immuno blot Immuno blot Redondo-Munoz J., Ugarte-Berzal E., Terol MJ., Van den Steen PE., Hernandez del Cerro M., Roderfeld M., Roeb E., Opdenakker G., Garcia-Marco JA., Garcia-Pardo A. Apoptosis - Interactions involving proteins with a function related to apoptosis DNA Pull-down Assay using biotinylated 22 nucleotide probe containing the specific binding site for STAT factors in the Mcl-1 promoter, and Streptavidin-Conjugated Beads. agarciapardo@cib.csic.es Cancer Cell (1535-6108) 2010 Only protein-protein interactions 6 imex curation Cancer - Interactions investigated in the context of cancer o43521-1 Bcl-2-like protein 11 Bim(EL) BCL2L11 BIM Bcl2-interacting mediator of cell death protein protein human Homo sapiens Human MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQGNPEGNHGGEGDSCPHGSPQGPLAPPASPGPFATRSPLFIFMRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASMRQAEPADMRPEIWIAQELRRIGDEFNAYYARRVFLNNYQAAEDHPRMVILRLLRYIVRLVWRMH mcl1_human Induced myeloid leukemia cell differentiation protein Mcl-1 Bcl-2-related protein EAT/mcl1 mcl1/EAT MCL1 BCL2L3 Bcl-2-like protein 3 protein protein human Homo sapiens Human MFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLTPDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDGVQRNHETAFQGMLRKLDIKNEDDVKSLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKHLKTINQESCIEPLAESITDVLVRTKRDWLVKQRGWDGFVEFFHVEDLEGGIRNVLLAFAGVAGVGAGLAYLIR lyn_human Tyrosine-protein kinase Lyn JTK8 Lck/Yes-related novel protein tyrosine kinase V-yes-1 Yamaguchi sarcoma viral related oncogene homolog p53Lyn p56Lyn LYN protein protein human Homo sapiens Human MGCIKSKGKDSLSDDGVDLKTQPVRNTERTIYVRDPTSNKQQRPVPESQLLPGQRFQTKDPEEQGDIVVALYPYDGIHPDDLSFKKGEKMKVLEEHGEWWKAKSLLTKKEGFIPSNYVAKLNTLETEEWFFKDITRKDAERQLLAPGNSAGAFLIRESETLKGSFSLSVRDFDPVHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKHYQKQADGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKRLGAGQFGEVWMGYYNNSTKVAVKTLKPGTMSVQAFLEEANLMKTLQHDKLVRLYAVVTREEPIYIITEYMAKGSLLDFLKSDEGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCFTIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMTALSQGYRMPRVENCPDELYDIMKMCWKEKAEERPTFDYLQSVLDDFYTATEGQYQQQP itb1_human Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta ITGB1 FNRB MDF2 MSK12 Glycoprotein IIa protein protein human Homo sapiens Human MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK ita4_human Integrin alpha-4 Integrin alpha-IV VLA-4 subunit alpha CD49 antigen-like family member D ITGA4 CD49D protein protein human Homo sapiens Human MAWEARREPGPRRAAVRETVMLLLCLGVPTGRPYNVDTESALLYQGPHNTLFGYSVVLHSHGANRWLLVGAPTANWLANASVINPGAIYRCRIGKNPGQTCEQLQLGSPNGEPCGKTCLEERDNQWLGVTLSRQPGENGSIVTCGHRWKNIFYIKNENKLPTGGCYGVPPDLRTELSKRIAPCYQDYVKKFGENFASCQAGISSFYTKDLIVMGAPGSSYWTGSLFVYNITTNKYKAFLDKQNQVKFGSYLGYSVGAGHFRSQHTTEVVGGAPQHEQIGKAYIFSIDEKELNILHEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIREEGRVFVYINSGSGAVMNAMETNLVGSDKYAARFGESIVNLGDIDNDGFEDVAIGAPQEDDLQGAIYIYNGRADGISSTFSQRIEGLQISKSLSMFGQSISGQIDADNNGYVDVAVGAFRSDSAVLLRTRPVVIVDASLSHPESVNRTKFDCVENGWPSVCIDLTLCFSYKGKEVPGYIVLFYNMSLDVNRKAESPPRFYFSSNGTSDVITGSIQVSSREANCRTHQAFMRKDVRDILTPIQIEAAYHLGPHVISKRSTEEFPPLQPILQQKKEKDIMKKTINFARFCAHENCSADLQVSAKIGFLKPHENKTYLAVGSMKTLMLNVSLFNAGDDAYETTLHVKLPVGLYFIKILELEEKQINCEVTDNSGVVQLDCSIGYIYVDHLSRIDISFLLDVSSLSRAEEDLSITVHATCENEEEMDNLKHSRVTVAIPLKYEVKLTVHGFVNPTSFVYGSNDENEPETCMVEKMNLTFHVINTGNSMAPNVSVEIMVPNSFSPQTDKLFNILDVQTTTGECHFENYQRVCALEQQKSAMQTLKGIVRFLSKTDKRLLYCIKADPHCLNFLCNFGKMESGKEASVHIQLEGRPSILEMDETSALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRPKRYFTIVIISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSNDD biotin_probe_mcl-1 biotinylated 22 nucleotide probe; the binding site for STAT factors in the Mcl-1 promoter dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human CCCTTTTATGGGAATACTTTTT stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D bimel-mcl1 886383 n/a 886386 unspecified role unspecified role prey prey n/a 886387 unspecified role unspecified role bait bait physical association physical association false false false In response to proMMP-9, Mcl-1 sequestered BH3-only proteins thus preventing mitochondrial damage and apoptosis. Fig 2C. itb1-ita4-lyn 886383 n/a 886388 unspecified role unspecified role prey prey n/a 886389 unspecified role unspecified role bait bait n/a 886390 unspecified role unspecified role prey prey association association false false false Anti-beta1 integrin antibodies pulled down the alpha4 integrin subunit as expected, plus one band of 55 kDa, which was recognized by an anti-Lyn antibody. Fig 3C. lyn-itab1 886383 n/a 886389 unspecified role unspecified role prey prey n/a 886388 unspecified role unspecified role bait bait region (possible) phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false After cell incubation on BSA, the beta1 integrin subunit remained bound to Lyn, but this association was almost completely lost upon incubation on proMMP-9. Cell binding to proMMP-9 clearly resulted in Lyn Tyr396 phosphorylation while incubation on BSA did not. Fig 3D. lyn-ita4-1 886383 n/a 886390 unspecified role unspecified role prey prey n/a 886389 unspecified role unspecified role prey prey n/a 886388 unspecified role unspecified role bait bait association association false false false Conversely, western blotting analyses of anti-Lyn immunoprecipitates revealed the presence of the alpha4 and beta1 integrin subunits. Fig S3C. lyn-ita4 886384 n/a 886388 unspecified role unspecified role neutral component neutral component n/a 886390 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig S3D. Colocalization of Lyn (green) with alpha4 and beta1 integrins (red) was further demonstrated. lyn-itb1 886384 n/a 886388 unspecified role unspecified role neutral component neutral component n/a 886389 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig S3D. Colocalization of Lyn (green) with alpha4 and beta1 integrins (red) was further demonstrated. stat3_probe 886385 n/a 886391 unspecified role unspecified role bait bait n/a 886392 unspecified role unspecified role prey prey physical association physical association false false false STAT3 was clearly increased in the nuclear fraction of proMMP-9-treated cells compared to BSA-treated cells. Moreover, STAT3 from proMMP-9-cultured cells was specifically pulled down by a Mcl-1 promoter probe, but not by a mutated or control DNA probe. Fig 4C.