IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ pavet-2010-1 Multivalent DR5 peptides activate the TRAIL death pathway and exert tumoricidal activity. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Pavet V., Beyrath J., Pardin C., Morizot A., Lechner M.C., Briand J.P., Wendland M., Maison W., Fournel S., Micheau O., Guichard G., Gronemeyer H. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin and features of TNFSF10, TNFR1 and TNF-alpha were provided by the author upon email request. hg@igbmc.fr Cancer Res. (0008-5472) 2010 Only protein-protein interactions imex curation pavet-2010-2 Multivalent DR5 peptides activate the TRAIL death pathway and exert tumoricidal activity. human-bjab Homo sapiens B-cell lymphoma cell line bjab B-cell lymphoma cell line, derived from the tumour of an exceptional African case of Burkitt's lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Pavet V., Beyrath J., Pardin C., Morizot A., Lechner M.C., Briand J.P., Wendland M., Maison W., Fournel S., Micheau O., Guichard G., Gronemeyer H. Apoptosis - Interactions involving proteins with a function related to apoptosis The BJAB cell line used in this experiment stably expresses TNFRSF10B (DR5). Origin and features of TNFSF10, TNFR1 and TNF-alpha were provided by the author upon email request. hg@igbmc.fr Cancer Res. (0008-5472) 2010 Only protein-protein interactions imex curation pavet-2010-3 Multivalent DR5 peptides activate the TRAIL death pathway and exert tumoricidal activity. human-bjab Homo sapiens B-cell lymphoma cell line bjab B-cell lymphoma cell line, derived from the tumour of an exceptional African case of Burkitt's lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Pavet V., Beyrath J., Pardin C., Morizot A., Lechner M.C., Briand J.P., Wendland M., Maison W., Fournel S., Micheau O., Guichard G., Gronemeyer H. Apoptosis - Interactions involving proteins with a function related to apoptosis The BJAB cell line used in this experiment stably does not express TNFRSF10B (DR5). Origin and features of TNFSF10, TNFR1 and TNF-alpha were provided by the author upon email request. hg@igbmc.fr Cancer Res. (0008-5472) 2010 Only protein-protein interactions imex curation tnf10_human Tumor necrosis factor ligand superfamily member 10 TNF-related apoptosis-inducing ligand Apo-2 ligand TNFSF10 APO2L TRAIL protein protein human Homo sapiens Human MAMMEVQGGPSLGQTCVLIVIFTVLLQSLCVAVTYVYFTNELKQMQDKYSKSGIACFLKEDDSYWDPNDEESMNSPCWQVKWQLRQLVRKMILRTSEETISTVQEKQQNISPLVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG tr10a_human Tumor necrosis factor receptor superfamily member 10A Death receptor 4 TNF-related apoptosis-inducing ligand receptor 1 TNFRSF10A APO2 DR4 TRAILR1 protein protein human Homo sapiens Human MAPPPARVHLGAFLAVTPNPGSAASGTEAAAATPSKVWGSSAGRIEPRGGGRGALPTSMGQHGPSARARAGRAPGPRPAREASPRLRVHKTFKFVVVGVLLQVVPSSAATIKLHDQSIGTQQWEHSPLGELCPPGSHRSEHPGACNRCTEGVGYTNASNNLFACLPCTACKSDEEERSPCTTTRNTACQCKPGTFRNDNSAEMCRKCSRGCPRGMVKVKDCTPWSDIECVHKESGNGHNIWVILVVTLVVPLLLVAVLIVCCCIGSGCGGDPKCMDRVCFWRLGLLRGPGAEDNAHNEILSNADSLSTFVSEQQMESQEPADLTGVTVQSPGEAQCLLGPAEAEGSQRRRLLVPANGADPTETLMLFFDKFANIVPFDSWDQLMRQLDLTKNEIDVVRAGTAGPGDALYAMLMKWVNKTGRNASIHTLLDALERMEERHAREKIQDLLVDSGKFIYLEDGTGSAVSLE tr10b_human Tumor necrosis factor receptor superfamily member 10B Death receptor 5 TNF-related apoptosis-inducing ligand receptor 2 UNQ160/PRO186 TNFRSF10B DR5 KILLER TRAILR2 TRICK2 ZTNFR9 protein protein human Homo sapiens Human MEQRGQNAPAASGARKRHGPGPREARGARPGPRVPKTLVLVVAAVLLLVSAESALITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKESGTKHSGEVPAVEETVTSSPGTPASPCSLSGIIIGVTVAAVVLIVAVFVCKSLLWKKVLPYLKGICSGGGGDPERVDRSSQRPGAEDNVLNEIVSILQPTQVPEQEMEVQEPAEPTGVNMLSPGESEHLLEPAEAERSQRRRLLVPANEGDPTETLRQCFDDFADLVPFDSWEPLMRKLGLMDNEIKVAKAEAAGHRDTLYTMLIKWVNKTGRDASVHTLLDALETLGERLAKQKIEDHLLSSGKFMYLEGNADSAMS tr10c_human Tumor necrosis factor receptor superfamily member 10C Decoy receptor 1 Decoy TRAIL receptor without death domain TNF-related apoptosis-inducing ligand receptor 3 TRAIL receptor without an intracellular domain Lymphocyte inhibitor of TRAIL Antagonist decoy receptor for TRAIL/Apo-2L DCR1 LIT TRAILR3 TRID UNQ321/PRO366 TNFRSF10C protein protein human Homo sapiens Human MARIPKTLKFVVVIVAVLLPVLAYSATTARQEEVPQQTVAPQQQRHSFKGEECPAGSHRSEHTGACNPCTEGVDYTNASNNEPSCFPCTVCKSDQKHKSSCTMTRDTVCQCKEGTFRNENSPEMCRKCSRCPSGEVQVSNCTSWDDIQCVEEFGANATVETPAAEETMNTSPGTPAPAAEETMNTSPGTPAPAAEETMTTSPGTPAPAAEETMTTSPGTPAPAAEETMITSPGTPASSHYLSCTIVGIIVLIVLLIVFV tr10d_human Tumor necrosis factor receptor superfamily member 10D Decoy receptor 2 TNF-related apoptosis-inducing ligand receptor 4 TRAIL receptor with a truncated death domain TNFRSF10D DCR2 TRAILR4 TRUNDD UNQ251/PRO288 protein protein human Homo sapiens Human MGLWGQSVPTASSARAGRYPGARTASGTRPWLLDPKILKFVVFIVAVLLPVRVDSATIPRQDEVPQQTVAPQQQRRSLKEEECPAGSHRSEYTGACNPCTEGVDYTIASNNLPSCLLCTVCKSGQTNKSSCTTTRDTVCQCEKGSFQDKNSPEMCRTCRTGCPRGMVKVSNCTPRSDIKCKNESAASSTGKTPAAEETVTTILGMLASPYHYLIIIVVLVIILAVVVVGFSCRKKFISYLKGICSGGGGGPERVHRVLFRRRSCPSRVPGAEDNARNETLSNRYLQPTQVSEQEIQGQELAELTGVTVESPEEPQRLLEQAEAEGCQRRRLLVPVNDADSADISTLLDASATLEEGHAKETIQDQLVGSEKLFYEEDEAGSATSCL trailmim/dr5 1t TRAIL mimicking peptide 1-trimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGRRQCVKL 3xtrailmim/dr5 1m linked through an adamantyl core. trailmim/dr5 2m TRAIL mimicking peptide 2-monomer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGKRQCVRL trailmim/dr5 3m TRAIL mimicking peptide 3-monomer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNKIGRRQCVRL trailmim/dr5 2d TRAIL mimicking peptide 2-dimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGKRQCVRL 2xtrailmim/dr5 2m linked through K10 using bis-succunimidyl carboxymehtoxyacetate. trailmim/dr5 3t TRAIL mimicking peptide 3-trimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNKIGRRQCVRL 3xtrailmim/dr5 3m linked through an adamantyl core. trailmim/dr5 2t TRAIL mimicking peptide 2-trimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGKRQCVRL 3xtrailmim/dr5 2m linked through an adamantyl core. trailmim/dr5 1m TRAIL mimicking peptide 1-monomer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGRRQCVKL trailmim/dr5 1d TRAIL mimicking peptide 1-dimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNRIGRRQCVKL 2xtrailmim/dr5 1m linked through K15 using bis-succunimidyl carboxymehtoxyacetate. trailmim/dr5 3d TRAIL mimicking peptide 3-dimer peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WDCLDNKIGRRQCVRL 2xtrailmim/dr5 3m linked through K7 using bis-succunimidyl carboxymehtoxyacetate. tnfa_human Tumor necrosis factor TNF TNFA TNFSF2 TNF-alpha Tumor necrosis factor ligand superfamily member 2 Cachectin protein protein human Homo sapiens Human MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL tnr1a_human Tumor necrosis factor receptor superfamily member 1A TNFRSF1A TNFAR TNFR1 p60 Tumor necrosis factor receptor 1 Tumor necrosis factor receptor type I p55 protein protein human Homo sapiens Human MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR casp8_human Caspase-8 CASP8 MCH5 ICE-like apoptotic protease 5 MORT1-associated ced-3 homolog FADD-homologous ICE/ced-3-like protease FADD-like ICE Apoptotic cysteine protease Apoptotic protease Mch-5 CAP4 protein protein human Homo sapiens Human MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQPTFTLRKKLVFPSD fadd_human FAS-associated death domain protein FAS-associating death domain-containing protein Mediator of receptor induced toxicity FADD MORT1 GIG3 Growth-inhibiting gene 3 protein protein protein human Homo sapiens Human MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRKLERVQSGLDLFSMLLEQNDLEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVICDNVGKDWRRLARQLKVSDTKIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVGALRSCQMNLVADLVQEVQQARDLQNRSGAMSPMSWNSDASTSEAS tnfrsf10a-tnfsf10-3 1242711 n/a 1242714 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1242715 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 1b and S1 tnfrsf10b-tnfsf10-4 1242711 n/a 1242716 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1242714 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1242711 1b and S1 tnfrsf10c-tnfsf10 1242711 n/a 1242717 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1242714 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1b and S1 tnfrsf10d-tnfsf10 1242711 n/a 1242714 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1242718 unspecified role unspecified role bait bait purified purified extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 1b and S1 tnfrsf10b-1t 1242711 n/a 1242719 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 1242711 S2 tnfrsf10b-2m 1242711 n/a 1242720 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 1242711 1b and S2 tnfrsf10b-3m 1242711 n/a 1242721 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 1242711 1b and S2 tnfrsf10b-2d 1242711 n/a 1242722 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 1242711 S1 and S2 tnfrsf10b-3t 1242711 n/a 1242723 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 1242711 S1 and S2 tnfrsf10b-2t 1242711 n/a 1242716 unspecified role unspecified role bait bait purified purified extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1242724 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1242711 S2 tnfrsf10b-1m 1242711 n/a 1242716 unspecified role unspecified role bait bait purified purified extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1242725 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1242711 1b and S2 tnfrsf10b-1d 1242711 n/a 1242726 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 1242711 S1 and S2 tnfrsf10b-3d 1242711 n/a 1242727 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role bait bait purified purified extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 1242711 S1 and S2 tnfsf10-tnfrsf10b-21 1242711 n/a 1242725 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242714 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1242716 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false 1242711 1c tnfsf10-tnfrsf10b-19 1242711 n/a 1242726 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1242714 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 1242711 1c tnfsf10-tnfrsf10b-25 1242711 n/a 1242720 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role prey prey purified purified extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1242714 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 1242711 1c tnfsf10-tnfrsf10b-22 1242711 n/a 1242722 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242714 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1242716 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false 1242711 1c tnfsf10-tnfrsf10b-20 1242711 n/a 1242724 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1242714 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 1242711 1c tnfsf10-tnfrsf10b-23 1242711 n/a 1242721 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1242714 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 1242711 1c tnfsf10-tnfrsf10b-24 1242711 n/a 1242727 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242714 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1242716 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false 1242711 1c tnfsf10-tnfrsf10b-18 1242711 n/a 1242723 competitor competitor competitor prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1242716 unspecified role unspecified role prey prey purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular portion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1242714 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 1242711 1c tnfrsf1a-tnf-2 1242711 n/a 1242728 unspecified role unspecified role prey prey purified purified yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 1242729 unspecified role unspecified role bait bait purified purified fc portion of human igg1 fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false extracellular domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false S1 casp8-fadd-13 1242712 n/a 1242715 unspecified role unspecified role prey prey n/a 1242716 unspecified role unspecified role prey prey n/a 1242730 unspecified role unspecified role bait bait n/a 1242731 unspecified role unspecified role prey prey association association false false false 2b The interaction depends on TNFSF10 (TRAIL) treatment of the cells. casp8-fadd-14 1242712 n/a 1242731 unspecified role unspecified role prey prey n/a 1242730 unspecified role unspecified role bait bait n/a 1242716 unspecified role unspecified role prey prey association association false false false 2b The interaction depends on treatment of the cells with TRAILmim/DR5 peptides (1m, 1d, 1t, 2d, 2t, 3m, 3d and 3t). casp8-fadd-15 1242713 n/a 1242730 unspecified role unspecified role bait bait n/a 1242731 unspecified role unspecified role prey prey n/a 1242715 unspecified role unspecified role prey prey association association false false false 2b The interaction depends on TNFSF10 (TRAIL) treatment of the cells.