IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ demon-2009-2 Proteome-wide substrate analysis indicates substrate exclusion as a mechanism to generate caspase-7 versus caspase-3 specificity. in vitro In vitro protease assay protease assay autoradiography autoradiography Demon D., Van Damme P., Vanden Berghe T., Deceuninck A., Van Durme J., Verspurten J., Helsens K., Impens F., Wejda M., Schymkowitz J., Rousseau F., Madder A., Vandekerckhove J., Declercq W., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis In this experiment, mouse macrophages were metabolically labeled by SILACm lysed and cleaved by recombinant caspases. The cleaved samples were then analyzed via COFRADIC-coupled mass spectrometry. peter.vandenabeele@dmbr.UGent.be Mol. Cell Proteomics (1535-9476) 2009 Only protein-protein interactions imex curation demon-2009-4 Proteome-wide substrate analysis indicates substrate exclusion as a mechanism to generate caspase-7 versus caspase-3 specificity. in vitro In vitro protease assay protease assay tag fluorescence tag visualisation by fluorescence tag fluorescence Demon D., Van Damme P., Vanden Berghe T., Deceuninck A., Van Durme J., Verspurten J., Helsens K., Impens F., Wejda M., Schymkowitz J., Rousseau F., Madder A., Vandekerckhove J., Declercq W., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis In this experiment, mouse macrophages were metabolically labeled by SILACm lysed and cleaved by recombinant caspases. The cleaved samples were then analyzed via COFRADIC-coupled mass spectrometry. peter.vandenabeele@dmbr.UGent.be Mol. Cell Proteomics (1535-9476) 2009 Only protein-protein interactions imex curation demon-2009-3 Proteome-wide substrate analysis indicates substrate exclusion as a mechanism to generate caspase-7 versus caspase-3 specificity. in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Demon D., Van Damme P., Vanden Berghe T., Deceuninck A., Van Durme J., Verspurten J., Helsens K., Impens F., Wejda M., Schymkowitz J., Rousseau F., Madder A., Vandekerckhove J., Declercq W., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis In this experiment, mouse macrophages were metabolically labeled by SILACm lysed and cleaved by recombinant caspases. The cleaved samples were then analyzed via COFRADIC-coupled mass spectrometry. peter.vandenabeele@dmbr.UGent.be Mol. Cell Proteomics (1535-9476) 2009 Only protein-protein interactions imex curation casp7_mouse Caspase-7 Casp7 Lice2 Mch3 Apoptotic protease Mch-3 Cysteine protease LICE2 protein protein mouse Mus musculus Mouse MTDDQDCAAELEKVDSSSEDGVDAKPDRSSIISSILLKKKRNASAGPVRTGRDRVPTYLYRMDFQKMGKCIIINNKNFDKATGMDVRNGTDKDAGALFKCFQNLGFEVTVHNDCSCAKMQDLLRKASEEDHSNSACFACVLLSHGEEDLIYGKDGVTPIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQADSGPINDIDANPRNKIPVEADFLFAYSTVPGYYSWRNPGKGSWFVQALCSILNEHGKDLEIMQILTRVNDRVARHFESQSDDPRFNEKKQIPCMVSMLTKELYFSR rs18_mouse 40S ribosomal protein S18 Rps18 Ke-3 protein protein mouse Mus musculus Mouse MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK mk67i_mouse MKI67 FHA domain-interacting nucleolar phosphoprotein Nifk Mki67ip Nucleolar protein interacting with the FHA domain of pKI-67 protein protein mouse Mus musculus Mouse MAGLAGPAKPSLALNPQEDSQFEKALTQIQGRTKKPQQKKKEKLNRGVVYLGHLPSTLSESHIYNYCAQFGDISRFRLSRSKRTGNSKGYAFVEFESEDVAKIVAETMDNYLFGERLLSCKFMPRKKVHKDLFSQRNALFHRPSFPAVKRYNRKRGHLQMLKMEYRFKKKEKLLRKKLAAKGIDYSFPSLVLPKPKNIAVAHRDSEGNQVLPDQKEGLSGEPRRKEKMMKEDISNNIPKKRKRSRRKKSSVDSQGPTPVCTPTFLERRKSQVMEVGGDKDDEIILKLPVPPVKEDTQKTPTSASPGGKRPRKRKSKQ mbb1a_mouse Myb-binding protein 1A Mybbp1a P160 Myb-binding protein of 160 kDa protein protein mouse Mus musculus Mouse MAEMKSPTKAEPATPAEAAQSDRHSLLEHSREFLDFFWDIAKPDQETRLRATEKLLEYLRTRPNDSEMKYALKRLITGLGVGREAARPCYSLALAQLLQSFEDIPLCDILDQIQEKYSLQAMNKAMMRPSLFANLFGVLALFQSGRLVKDKEALMKSVQLLKILSQHPNHLQGQPIKALVDILSEVPESMFQEILPKVLKGNMKVILRSPKYLELFLLAKQRVPTKLESLMGSVDLFSEDNIPSLVNILKVAANSVKKEHKLPNVALDLLRLALKESRFELFWKKVLEEGLLKNPSWTSSYMCFRLLGASLPLLSEEQLQLVMRGDLIRHFGENMVISKPQNLFKIIPEISTYVGTFLEGCQDDPKRQLTMMVAFTTITNQGLPVMPTFWRVTRFLNAEALQSYVAWLRDMFLQPDLNSLVDFSTANQKRAQDASLNVPERAVFRLRKWIIHRLVSLVDHLHLEKDEAVVEQIARFCLFHAFFKTKKATPQIPETKQHFSFPLDDRNRGVFVSAFFSLLQTLSVKFRQTPDLAENGKPWTYRLVQLADMLLNHNRNVTSVTSLTTQQRQAWDQMMSTLKELEARSSETRAIAFQHLLLLVGLHIFKSPAESCDVLGDIQTCIKKSMEQNPRRSRSRAKASQEPVWVEVMVEILLSLLAQPSNLMRQVVRSVFGHICPHLTPRCLQLILAVLSPVTNEDEDDNVVVTDDADEKQLQHGEDEDSDNEDNKNSESDMDSEDGEESEEEDRDKDVDPGFRQQLMEVLKAGNALGGVDNEEEEELGDEAMMALDQNLASLFKEQKMRIQARNEEKNKLQKEKKLRRDFQIRALDLIEVLVTKQPEHPLILELLEPLLNVIQHSMRSKGSTKQEQDLLHKTARIFMHHLCRARRYCHEVGPCAEALHAQVERLVQQAGSQADASVALYYFNASLYLLRVLKGNTNKRHQDGHKLHGADTEDSEDQAANCLDLDFVTRVYSASLESLLTKRNSSLTVPMFLSLFSRYPVICKNLLPVLAQHVAGPSRPRHQAQACLMLQKTLSARELRVCFEDPEWEQLITQLLGKATQTLQTLGEAQSKGEHQKELSILELLNTLLRTVNHEKLSVDLTAPLGVLQSKQQKLQQSLQQGNHSSGSNRLYDLYWQAMRMLGVQRPKSEKKNAKDIPSDTQSPVSTKRKKKGFLPETKKRKKLKSEGTTPEKNAASQQDAVTEGAMPAATGKDQPPSTGKKKRKRVKASTPSQVNGITGAKSPAPSNPTLSPSTPAKTPKLQKKKEKLSQVNGATPVSPIEPESKKHHQEALSTKEVIRKSPHPQSALPKKRARLSLVSRSPSLLQSGVKKRRVASRRVQTP rl28_mouse 60S ribosomal protein L28 Rpl28 protein protein mouse Mus musculus Mouse MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVMKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILRSQKPVVVKRKRTRPTKSS nucks_mouse Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 Nucks1 Nucks JC7 protein protein mouse Mus musculus Mouse MSRPVRNRKVVDYSQFQESDDADEDYGRDSGPPAKKIRSSPREAKNKRRSGKNSQEDSEDSEEKDVKTKKDDSHSAEDSEDEKDDHKNVRQQRQAASKAASKQREMLLEDVGSEEEPEEDDEAPFQEKDSGSDEDFLMEDDDDSDYGSSKKKNKKMVKKSKPERKEKKMPKPRLKATVTPSPVKGKAKVGRPTASKKSKEKTPSPKEEDEEAESPPEKKSGDEGSEDEASSGED hdgf_mouse Hepatoma-derived growth factor Hdgf Tdrm1 protein protein mouse Mus musculus Mouse MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCAAEPEVEPEAHEGDGDKKGSAEGSSDEEGKLVIDEPAKEKNEKGTLKRRAGDVLEDSPKRPKESGDHEEEDKEIAALEGERPLPVEVEKNSTPSEPDSGQGPPAEEEEGEEEAAKEEAEAQGVRDHESL wasc2_mouse WASH complex subunit 2 Washc2 D6Wsu116e Fam21 Kiaa0592 protein protein mouse Mus musculus Mouse MNRTSPDSERPPASEPVWERPWSVEEIRRSSQNWSLAADAGLLQFLQEFSQQTISRTHEIKKQVDGLIQETKATHCRLHNVFNDFLMLSNTQFIENRVYDEEVEEQVLKAEAEKAEQEKTREQKEIDLIPKVQEAVNYGLQVLDSAFEQLDIKAGNSDSEEDDANERVDLILEPKDLYIDRPLPYLIGSKLFMEQEDVGLGELSSEEGSVGSDRGSIVDSEDEKEEEESDEDFASHSDNDQNQHTTQISDEEEDDDGDLFADSEKEGDDIEDIEESAKSKRPTSFADELAARIKGDISNQRKEGQTDGKPQKTVKEKKERRTPADDEEDILFPPPTLTDEDFSPFGSRGGLFSNGQGLFDDEDESDLFKEAPRARPAQAPVSEELPPSPKPGKKIPAGAVSVLLGHPDVSGSTSAPSLKELQKHGQPTPGKSSHLPTPAGLFDDDDNDNDEDDNNFFMPSSSKPSKTDKVKSTAIIFDDDEGDLFKEKAEALPAASVSQTHESKTRADKTIALPSSKNLKLVSETKTQKGLFSDEEDSEDLFSSQSSSKPKSASLPSSQPPTSVSLFGDEDEEDSLFGSAAAKKQTSSLQPQSQEKAKPSEQPSKKTSALLFSSDEEDQWNIADSHTKLASDNKSKGELWDSGATQGQEAKAVKKTNLFEDDDDDEVDLFAIAKDSQKKTQRTSLLFEDDAESGSSLFGLPPTSVPSATTKKESVPKVPLLFSDEEDSEVPSGVKPEDLKVDNARVSPEVGSADVASIAQKEGLLPASDQEAGGPSDIFSSSSPLDKGAKGRTRTVLSLFDEDEDKVEDESSTCAPQDGREKGLKTDSRPKSTGVFQDEELLFSHKLQKDNDPDVDLFAGTKKIRSSVPSGGSLFGDDEDDDLFSSAKTQPVVPEKKGTLKKDHPVSLKNQDPLDSTQGSKEKSTWKTEPAQDSSGLTPFKSREPSSRIGKIQANLAINPAALLPTVALQIPGTKPVSSELAFPSSEPGRSHILESVPTLPGSVEAGVSFDLPAQADTLHSANKSRVKVRGKRRPQTRAARRLAAQESSEAEDVTVDRGPVAQLSSSPVLPNGHQPLLQPRMASGQTSSETATAPPWEGGPVLSAADRSFFVKSRPQTGNEADLFDSGDIFPKSRGSQSVEGAGVMAGEPPSHSSGGRKEKSLAFPDLSEGSSTEDLFQSVKPRAAKNRNPFPLLEDEEDLFADPRGKKNERKPDSHQDSVSKTHDIFEDDIFATEAIKPFPKKREKGRTLEPNLFDDNIDIFADLTVKPKEKSKKKVAAKSMFDDDTDDIFSSGLQAKASKPKSQSAEAASEQRSEHKVASIFDDPLNAFGSQ acta_mouse Actin, aortic smooth muscle Acta2 Actsa Actvs Alpha-actin-2 protein protein mouse Mus musculus Mouse MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF kpym_mouse Pyruvate kinase PKM Pkm Pk3 Pkm2 Pykm Pyruvate kinase muscle isozyme protein protein mouse Mus musculus Mouse MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP casp3_mouse Caspase-3 Casp3 Cpp32 Apopain Cysteine protease CPP32 Protein Yama SREBP cleavage activity 1 LICE protein protein mouse Mus musculus Mouse MENNKTSVDSKSINNFEVKTIHGSKSVDSGIYLDSSYKMDYPEMGICIIINNKNFHKSTGMSSRSGTDVDAANLRETFMGLKYQVRNKNDLTREDILELMDSVSKEDHSKRSSFVCVILSHGDEGVIYGTNGPVELKKLTSFFRGDYCRSLTGKPKLFIIQACRGTELDCGIETDSGTDEEMACQKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCSMLKLYAHKLEFMHILTRVNRKVATEFESFSLDSTFHAKKQIPCIVSMLTKELYFYH parp1_mouse Poly [ADP-ribose] polymerase 1 Poly[ADP-ribose] synthase 1 NAD(+) ADP-ribosyltransferase 1 Parp1 Adprp Adprt Adprt1 ADP-ribosyltransferase diphtheria toxin-like 1 DNA ADP-ribosyltransferase PARP1 Protein poly-ADP-ribosyltransferase PARP1 protein protein mouse Mus musculus Mouse MAEASERLYRVQYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGQSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVAGKGQDGSGGKAEKTLGDFAAEYAKSNRSMCKGCLEKIEKGQMRLSKKMVDPEKPQLGMIDRWYHPTCFVKKRDELGFRPEYSASQLKGFSLLSAEDKEALKKQLPAIKNEGKRKGDEVDGTDEVAKKKSRKETDKYSKLEKALKAQNELIWNIKDELKKACSTNDLKELLIFNQQQVPSGESAILDRVADGMAFGALLPCKECSGQLVFKSDAYYCTGDVTAWTKCMVKTQNPSRKEWVTPKEFREISYLKKLKVKKQDRIFPPESSAPITVHWPLSVTSAPTAVNSSAPADKPLSNMKILTLGKLSQNKDEAKAVIEKLGGKLTGSANKASLCISIKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQDLLSAHSLSPWGAEVKAEPGEVVAPRGKSAAPSKKSKGCFKEEGVNKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKESRYWIFRSWGRLGTVIGSNKLEQMPSKEEAVEQFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVKPGTKSKLPKPVQELVGMIFDVDSMKKALVEYEIDLQKMPLGKLSRRQIQAAYSILSEVQQPVSQGSSESQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEVIRKYVKNTHATTHNAYDLEVIDIFKIEREGESQRYKPFRQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLIMLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSASITLEGVEVPLGTGIPSGVNDTALLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW casp7-rps18-1 1246778 n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1246782 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage false false false 2a, 5c and 6e casp7-mk67i-1 1246778 n/a 1246783 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 2b casp7-mybbp1a 1246778 n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1246784 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage false false false 2c casp7-rl28 1246778 n/a 1246785 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 2d casp7-nucks 1246778 n/a 1246786 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 2f casp7-hdgf 1246778 n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1246787 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage false false false 2f casp7-fam21 1246778 n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1246788 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage false false false 2h casp7-acta-1 1246778 n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1246789 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage false false false 2i casp7-kpym-1 1246778 n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1246790 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage false false false 2j casp3-kpym-1 1246778 n/a 1246790 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 2j casp3-acta2-1 1246778 n/a 1246789 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 2i casp3-fam21-1 1246778 n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1246788 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage false false false 2h casp3-hdgf-1 1246778 n/a 1246787 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 2g casp3-nucks-1 1246778 n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1246786 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage false false false 2f casp3-rps18-2 1246778 n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1246782 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P62270:p.Lys88Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage false false false 5d casp7-parp1 1246778 n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1246792 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false 5g and 6f casp3-parp1-1 1246778 n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1246792 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified P11103:p.Gly210Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false 5h and 6h casp7-parp1-1 1246779 n/a 1246792 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified tetrapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 1246779 1246779 table 1 casp3-parp1-2 1246779 n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1246792 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified tetrapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false 1246779 1246779 table 1 casp7-rps18-3 1246779 n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1246782 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified p6-p5' undecapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false tetrapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false 1246779 1246779 table 1 casp7-rps18-4 1246779 n/a 1246782 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false pentapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P62270:p.Lys88Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 1246779 1246779 table 1 casp3-rps18-5 1246779 n/a 1246782 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified tetrapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false hexapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P62270:p.Gln87Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 1246779 1246779 table 1 casp3-rps18-6 1246779 n/a 1246782 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified P62270:p.Lys88Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false pentapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 1246779 1246779 table 1 casp7-rps18-2 1246780 n/a 1246782 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified p6-p5' undecapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pentapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 4a, 5a, 6a, 6b, 6c, 6d and 6e casp3-parp 1246780 n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1246792 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified P11103:p.Gly210Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false p6-p5' undecapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false tetrapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false 5f and 6h casp7-parp 1246780 n/a 1246781 enzyme enzyme unspecified role unspecified role purified purified casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1246792 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified p6-p5' undecapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false tetrapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false 5e and 6f casp3-rps18-1 1246780 n/a 1246782 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified p6-p5' undecapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P62270:p.Lys88Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) 1246994 1246995 protein cleavage protein cleavage false false false 5b casp3-rps18-7 1246780 n/a 1246791 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1246782 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified p6-p2' octapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pentapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false 6c, 6d and 6g