IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ jin-2009-4 Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling. human-h460 Homo sapiens non-small-cell lung cancer cell line H460 h460 human non-small-cell lung cancer cell line H460 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jin Z., Li Y., Pitti R., Lawrence D., Pham VC., Lill JR., Ashkenazi A. Apoptosis - Interactions involving proteins with a function related to apoptosis aa@gene.com Cell (0092-8674) 2009 Only protein-protein interactions 19 imex curation jin-2009-7 Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling. human-h460 Homo sapiens non-small-cell lung cancer cell line H460 h460 human non-small-cell lung cancer cell line H460 confocal microscopy confocal microscopy immunostaining immunostaining Jin Z., Li Y., Pitti R., Lawrence D., Pham VC., Lill JR., Ashkenazi A. Apoptosis - Interactions involving proteins with a function related to apoptosis aa@gene.com Cell (0092-8674) 2009 Only protein-protein interactions 19 imex curation jin-2009-1 Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling. human-h460 Homo sapiens non-small-cell lung cancer cell line H460 h460 human non-small-cell lung cancer cell line H460 anti bait coip anti bait coimmunoprecipitation anti bait coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Jin Z., Li Y., Pitti R., Lawrence D., Pham VC., Lill JR., Ashkenazi A. Apoptosis - Interactions involving proteins with a function related to apoptosis aa@gene.com Cell (0092-8674) 2009 Only protein-protein interactions 19 imex curation jin-2009-2 Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling. human-h460 Homo sapiens non-small-cell lung cancer cell line H460 h460 human non-small-cell lung cancer cell line H460 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Jin Z., Li Y., Pitti R., Lawrence D., Pham VC., Lill JR., Ashkenazi A. Apoptosis - Interactions involving proteins with a function related to apoptosis aa@gene.com Cell (0092-8674) 2009 Only protein-protein interactions 19 imex curation jin-2009-9 Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Jin Z., Li Y., Pitti R., Lawrence D., Pham VC., Lill JR., Ashkenazi A. Apoptosis - Interactions involving proteins with a function related to apoptosis origin of CUL3 confirmed by author. aa@gene.com Cell (0092-8674) 2009 Only protein-protein interactions 19 imex curation jin-2009-3 Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Jin Z., Li Y., Pitti R., Lawrence D., Pham VC., Lill JR., Ashkenazi A. Apoptosis - Interactions involving proteins with a function related to apoptosis aa@gene.com Cell (0092-8674) 2009 Only protein-protein interactions 19 imex curation jin-2009-5 Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Jin Z., Li Y., Pitti R., Lawrence D., Pham VC., Lill JR., Ashkenazi A. Apoptosis - Interactions involving proteins with a function related to apoptosis aa@gene.com Cell (0092-8674) 2009 Only protein-protein interactions 19 imex curation jin-2009-6 Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference confocal microscopy confocal microscopy immunostaining immunostaining Jin Z., Li Y., Pitti R., Lawrence D., Pham VC., Lill JR., Ashkenazi A. Apoptosis - Interactions involving proteins with a function related to apoptosis aa@gene.com Cell (0092-8674) 2009 Only protein-protein interactions 19 imex curation jin-2009-8 Cullin3-based polyubiquitination and p62-dependent aggregation of caspase-8 mediate extrinsic apoptosis signaling. human-h2122 Human lung carcinoma cell line h2122 Lung carcinoma cell line H2122 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jin Z., Li Y., Pitti R., Lawrence D., Pham VC., Lill JR., Ashkenazi A. Apoptosis - Interactions involving proteins with a function related to apoptosis aa@gene.com Cell (0092-8674) 2009 Only protein-protein interactions 19 imex curation cul3_human Cullin-3 CUL3 KIAA0617 protein protein human Homo sapiens Human MSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA casp8_human Caspase-8 CASP8 MCH5 ICE-like apoptotic protease 5 MORT1-associated ced-3 homolog FADD-homologous ICE/ced-3-like protease FADD-like ICE Apoptotic cysteine protease Apoptotic protease Mch-5 CAP4 protein protein human Homo sapiens Human MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQPTFTLRKKLVFPSD tr10a_human Tumor necrosis factor receptor superfamily member 10A Death receptor 4 TNF-related apoptosis-inducing ligand receptor 1 TNFRSF10A APO2 DR4 TRAILR1 protein protein human Homo sapiens Human MAPPPARVHLGAFLAVTPNPGSAASGTEAAAATPSKVWGSSAGRIEPRGGGRGALPTSMGQHGPSARARAGRAPGPRPAREASPRLRVHKTFKFVVVGVLLQVVPSSAATIKLHDQSIGTQQWEHSPLGELCPPGSHRSEHPGACNRCTEGVGYTNASNNLFACLPCTACKSDEEERSPCTTTRNTACQCKPGTFRNDNSAEMCRKCSRGCPRGMVKVKDCTPWSDIECVHKESGNGHNIWVILVVTLVVPLLLVAVLIVCCCIGSGCGGDPKCMDRVCFWRLGLLRGPGAEDNAHNEILSNADSLSTFVSEQQMESQEPADLTGVTVQSPGEAQCLLGPAEAEGSQRRRLLVPANGADPTETLMLFFDKFANIVPFDSWDQLMRQLDLTKNEIDVVRAGTAGPGDALYAMLMKWVNKTGRNASIHTLLDALERMEERHAREKIQDLLVDSGKFIYLEDGTGSAVSLE tr10b_human Tumor necrosis factor receptor superfamily member 10B Death receptor 5 TNF-related apoptosis-inducing ligand receptor 2 UNQ160/PRO186 TNFRSF10B DR5 KILLER TRAILR2 TRICK2 ZTNFR9 protein protein human Homo sapiens Human MEQRGQNAPAASGARKRHGPGPREARGARPGPRVPKTLVLVVAAVLLLVSAESALITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKESGTKHSGEVPAVEETVTSSPGTPASPCSLSGIIIGVTVAAVVLIVAVFVCKSLLWKKVLPYLKGICSGGGGDPERVDRSSQRPGAEDNVLNEIVSILQPTQVPEQEMEVQEPAEPTGVNMLSPGESEHLLEPAEAERSQRRRLLVPANEGDPTETLRQCFDDFADLVPFDSWEPLMRKLGLMDNEIKVAKAEAAGHRDTLYTMLIKWVNKTGRDASVHTLLDALETLGERLAKQKIEDHLLSSGKFMYLEGNADSAMS sqstm_human Sequestosome-1 SQSTM1 ORCA OSIL Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 EBI3-associated protein of 60 kDa protein protein human Homo sapiens Human MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL otu7b_human OTU domain-containing protein 7B Zinc finger protein Cezanne Zinc finger A20 domain-containing protein 1 Cellular zinc finger anti-NF-kappa-B protein OTUD7B ZA20D1 protein protein human Homo sapiens Human MTLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDVNAALSDFEQLRQVHAGNLPPSFSEGSGGSRTPEKGFSDREPTRPPRPILQRQDDIVQEKRLSRGISHASSSIVSLARSHVSSNGGGGGSNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLIEQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEKGVEKEALKRRWRWQQTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALVSMEQKENTKEQAVIPLTDSEYKLLPLHFAVDPGKGWEWGKDDSDNVRLASVILSLEVKLHLLHSYMNVKWIPLSSDAQAPLAQPESPTASAGDEPRSTPESGDSDKESVGSSSTSNEGGRRKEKSKRDREKDKKRADSVANKLGSFGKTLGSKLKKNMGGLMHSKGSKPGGVGTGLGGSSGTETLEKKKKNSLKSWKGGKEEAAGDGPVSEKPPAESVGNGGSKYSQEVMQSLSILRTAMQGEGKFIFVGTLKMGHRHQYQEEMIQRYLSDAEERFLAEQKQKEAERKIMNGGIGGGPPPAKKPEPDAREEQPTGPPAESRAMAFSTGYPGDFTIPRPSGGGVHCQEPRRQLAGGPCVGGLPPYATFPRQCPPGRPYPHQDSIPSLEPGSHSKDGLHRGALLPPPYRVADSYSNGYREPPEPDGWAGGLRGLPPTQTKCKQPNCSFYGHPETNNFCSCCYREELRRREREPDGELLVHRF ubb_human Polyubiquitin-B UBB protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGC caspa_human Caspase-10 ICE-like apoptotic protease 4 Apoptotic protease Mch-4 FAS-associated death domain protein interleukin-1B-converting enzyme 2 CASP10 MCH4 protein protein human Homo sapiens Human MKSQGQHWYSSSDKNCKVSFREKLLIIDSNLGVQDVENLKFLCIGLVPNKKLEKSSSASDVFEHLLAEDLLSEEDPFFLAELLYIIRQKKLLQHLNCTKEEVERLLPTRQRVSLFRNLLYELSEGIDSENLKDMIFLLKDSLPKTEMTSLSFLAFLEKQGKIDEDNLTCLEDLCKTVVPKLLRNIEKYKREKAIQIVTPPVDKEAESYQGEEELVSQTDVKTFLEALPQESWQNKHAGSNGNRATNGAPSLVSRGMQGASANTLNSETSTKRAAVYRMNRNHRGLCVIVNNHSFTSLKDRQGTHKDAEILSHVFQWLGFTVHIHNNVTKVEMEMVLQKQKCNPAHADGDCFVFCILTHGRFGAVYSSDEALIPIREIMSHFTALQCPRLAEKPKLFFIQACQGEEIQPSVSIEADALNPEQAPTSLQDSIPAEADFLLGLATVPGYVSFRHVEEGSWYIQSLCNHLKKLVPRMLKFLEKTMEIRGRKRTVWGAKQISATSLPTAISAQTPRPPMRRWSSVS cflar_human CASP8 and FADD-like apoptosis regulator Cellular FLICE-like inhibitory protein Caspase-eight-related protein Caspase-like apoptosis regulatory protein MACH-related inducer of toxicity Caspase homolog Inhibitor of FLICE FADD-like antiapoptotic molecule 1 Usurpin CFLAR CASH CLARP MRIT CASP8AP1 protein protein human Homo sapiens Human MSAEVIHQVEEALDTDEKEMLLFLCRDVAIDVVPPNVRDLLDILRERGKLSVGDLAELLYRVRRFDLLKRILKMDRKAVETHLLRNPHLVSDYRVLMAEIGEDLDKSDVSSLIFLMKDYMGRGKISKEKSFLDLVVELEKLNLVAPDQLDLLEKCLKNIHRIDLKTKIQKYKQSVQGAGTSYRNVLQAAIQKSLKDPSNNFRLHNGRSKEQRLKEQLGAQQEPVKKSIQESEAFLPQSIPEERYKMKSKPLGICLIIDCIGNETELLRDTFTSLGYEVQKFLHLSMHGISQILGQFACMPEHRDYDSFVCVLVSRGGSQSVYGVDQTHSGLPLHHIRRMFMGDSCPYLAGKPKMFFIQNYVVSEGQLEDSSLLEVDGPAMKNVEFKAQKRGLCTVHREADFFWSLCTADMSLLEQSHSSPSLYLQCLSQKLRQERKRPLLDLHIELNGYMYDWNSRVSAKEKYYVWLQHTLRKKLILSYT tnf10_human Tumor necrosis factor ligand superfamily member 10 TNF-related apoptosis-inducing ligand Apo-2 ligand TNFSF10 APO2L TRAIL protein protein human Homo sapiens Human MAMMEVQGGPSLGQTCVLIVIFTVLLQSLCVAVTYVYFTNELKQMQDKYSKSGIACFLKEDDSYWDPNDEESMNSPCWQVKWQLRQLVRKMILRTSEETISTVQEKQQNISPLVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG fadd_human FAS-associated death domain protein FAS-associating death domain-containing protein Mediator of receptor induced toxicity FADD MORT1 GIG3 Growth-inhibiting gene 3 protein protein protein human Homo sapiens Human MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRKLERVQSGLDLFSMLLEQNDLEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVICDNVGKDWRRLARQLKVSDTKIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVGALRSCQMNLVADLVQEVQQARDLQNRSGAMSPMSWNSDASTSEAS ub2l3_human Ubiquitin-conjugating enzyme E2 L3 Ubiquitin-protein ligase L3 Ubiquitin carrier protein L3 UbcH7 Ubiquitin-conjugating enzyme E2-F1 L-UBC UBE2L3 UBCE7 UBCH7 E2 ubiquitin-conjugating enzyme L3 protein protein human Homo sapiens Human MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD ub2v1_human Ubiquitin-conjugating enzyme E2 variant 1 CROC-1 TRAF6-regulated IKK activator 1 beta Uev1A P/OKcl.19 UBE2V1 CROC1 UBE2V UEV1 protein protein human Homo sapiens Human MAATTGSGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQCYSN ub2d1_human Ubiquitin-conjugating enzyme E2 D1 Ubiquitin-protein ligase D1 Ubiquitin carrier protein D1 UbcH5 Ubiquitin-conjugating enzyme E2-17 kDa 1 Ubiquitin-conjugating enzyme E2(17)KB 1 UBC4/5 homolog Stimulator of Fe transport UBE2D1 UBCH5 UBCH5A SFT UBC5A (E3-independent) E2 ubiquitin-conjugating enzyme D1 E2 ubiquitin-conjugating enzyme D1 protein protein human Homo sapiens Human MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM casp8-cul3-1 Interaction between CASP8 and CUl3 determined by coimmunopreciptiation 828928 n/a 828937 unspecified role unspecified role prey prey region (possible) neddylated lysine neddylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 828938 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2D casp8-cul3-2 Interaction between CASP8 and CUl3 determined by coimmunopreciptiation 828928 n/a 828937 unspecified role unspecified role bait bait region (possible) neddylated lysine neddylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 828938 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2E tnfrsf10a-sqstm1-1 Interactors of TNFRSF10A 828928 n/a 828939 unspecified role unspecified role bait bait n/a 828940 unspecified role unspecified role prey prey n/a 828941 unspecified role unspecified role prey prey n/a 828938 unspecified role unspecified role prey prey association association false false false Fig. 6A casp8-sqstm1-1 Interaction between CASP8 and SQSTM1 identified by coimmunoprecipitation 828928 n/a 828941 unspecified role unspecified role bait bait n/a 828938 unspecified role unspecified role prey prey association association false false false Fig. 6A CUL3 knockdown attenuated the association of p62 with the DISC. casp8-otud7b-1 Interaction between CASP8 and OTUD7B determined by coimmunopreciptiation 828928 n/a 828942 unspecified role unspecified role prey prey n/a 828938 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2D TNFSF10 casp8-tnfrsf10a-1 Colocalisation of CASP8 and TNFRSF10A 828929 n/a 828938 unspecified role unspecified role neutral component neutral component n/a 828939 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 7A-C, 7E TNFSF10 casp8-tnfrsf10a-2 Colocalisation of CASP8, SQSTM1 and TNFRSF10A 828929 n/a 828941 unspecified role unspecified role neutral component neutral component n/a 828938 unspecified role unspecified role neutral component neutral component n/a 828939 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 7E, Supp. Fig. 12 TNFSF10 casp8-sqstm1-2 Colocalisation of CASP8 and SQSTM1 828929 n/a 828938 unspecified role unspecified role neutral component neutral component n/a 828941 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 7E, Supp. Fig. 12 TNFSF10 rps27a-sqstm1-3 Colocalisation of RPS27A and SQSTM1 828929 n/a 828941 unspecified role unspecified role neutral component neutral component n/a 828943 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp. Fig. 11 TNFSF10 tnfrsf10a-tnfsf10-1 Interactors of TNFRSF10A 828930 n/a 828944 unspecified role unspecified role prey prey n/a 828945 unspecified role unspecified role prey prey n/a 828946 unspecified role unspecified role prey prey n/a 828940 unspecified role unspecified role prey prey n/a 828947 unspecified role unspecified role prey prey n/a 828938 unspecified role unspecified role prey prey n/a 828939 unspecified role unspecified role bait bait n/a 828937 unspecified role unspecified role prey prey n/a 828942 unspecified role unspecified role prey prey n/a 828941 unspecified role unspecified role prey prey association association false false false TNFSF10 (1 ug/ml) Fig. 2A, Supp. Fig. 8A tnfrsf10b-tnfsf10-2 Interactors of TNFRSF10B 828930 n/a 828942 unspecified role unspecified role prey prey n/a 828941 unspecified role unspecified role prey prey n/a 828947 unspecified role unspecified role prey prey n/a 828937 unspecified role unspecified role prey prey n/a 828938 unspecified role unspecified role prey prey n/a 828946 unspecified role unspecified role prey prey n/a 828944 unspecified role unspecified role prey prey n/a 828940 unspecified role unspecified role prey prey n/a 828945 unspecified role unspecified role prey prey n/a 828939 unspecified role unspecified role bait bait association association false false false TNFSF10 (1 ug/ml) Supp. Fig. 8A tnfsf10-cul3-1 Interaction between TNFSF10 and CUl3 determined by coimmunopreciptiation 828931 n/a 828937 unspecified role unspecified role prey prey region (possible) neddylated lysine neddylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 828946 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2C tnfsf10-cul3-3 Interactors of TNFSF10 828931 n/a 828938 unspecified role unspecified role prey prey n/a 828940 unspecified role unspecified role prey prey n/a 828946 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 828947 unspecified role unspecified role prey prey n/a 828939 unspecified role unspecified role prey prey association association false false false Fig. 3C casp8-cul3-5 Interaction between CASP8 and CUl3 determined by coimmunopreciptiation 828932 n/a 828937 unspecified role unspecified role prey prey region (possible) neddylated lysine neddylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 828938 unspecified role unspecified role bait bait n-terminal t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 2G CASP8 is a non-cleavable mutant - details not given in the paper. casp8-cul3-6 Interaction between CASP8 and CUl3 determined by coimmunopreciptiation 828932 n/a 828937 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 828938 unspecified role unspecified role prey prey n-terminal t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 2F CASP8 is a non-cleavable mutant - details not given in the paper. tnfsf10-cul3-2 Interaction between TNFSF10 and CUl3 determined by coimmunopreciptiation 828933 n/a 828937 unspecified role unspecified role prey prey region (possible) neddylated lysine neddylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 828946 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2C casp8-cul3-3 Ubiquination of CASP8 828934 n/a 828937 unspecified role unspecified role ancillary ancillary human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 n/a 828943 unspecified role unspecified role ancillary ancillary n/a 828938 enzyme target enzyme target substrate substrate neutral component neutral component n-terminal t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro n/a 828948 unspecified role unspecified role ancillary ancillary n/a 828949 unspecified role unspecified role ancillary ancillary n/a 828950 enzyme enzyme neutral component neutral component ubiquitination ubiquitination reaction ubiquitination false false false Additional CUL3 binding proteins may have been present in the assay but were not identified. Fig. 4D Ubiquitination of CASP8 CASP8 is a non-cleavable mutant - details not given in paper. casp8-sqstm1-3 Colocalisation of CASP8 and SQSTM1 828935 n/a 828938 unspecified role unspecified role neutral component neutral component n/a 828941 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 6F casp8-cul3-4 Interaction between CASP8 and CUl3 determined by coimmunopreciptiation 828936 n/a 828938 unspecified role unspecified role bait bait n/a 828937 unspecified role unspecified role prey prey region (possible) neddylated lysine neddylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp. Fig. S4B TNFSF10