UniProt UniProt http://www.uniprot.org jacobs-2008-2 SIRT3 interacts with the daf-16 homolog FOXO3a in the Mitochondria, as well as increases FOXO3a Dependent Gene expression. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Jacobs K.M., Pennington J.D., Bisht K.S., Aykin-Burns N., Kim H.S., Mishra M., Sun L., Nguyen P., Ahn B.H., Leclerc J., Deng C.X., Spitz D.R., Gius D. Diabetes - Interactions investigated in the context of Diabetes HCT116 cells, which contain undetectable levels of endogenous SIRT3 by western analysis, were stably transfected with p-myc-hSIRT3-wt. giusd@mail.nih.gov Int. J. Biol. Sci. 2008 Apoptosis - Interactions involving proteins with a function related to apoptosis jacobs-2008-1 SIRT3 interacts with the daf-16 homolog FOXO3a in the Mitochondria, as well as increases FOXO3a Dependent Gene expression. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jacobs K.M., Pennington J.D., Bisht K.S., Aykin-Burns N., Kim H.S., Mishra M., Sun L., Nguyen P., Ahn B.H., Leclerc J., Deng C.X., Spitz D.R., Gius D. Diabetes - Interactions investigated in the context of Diabetes HCT116 cells, which contain undetectable levels of endogenous SIRT3 by western analysis, were stably transfected with p-myc-hSIRT3-wt. giusd@mail.nih.gov Int. J. Biol. Sci. 2008 Apoptosis - Interactions involving proteins with a function related to apoptosis sodm_human_gene SODM_HUMAN_GENE/SOD2/Superoxide dismutase [Mn], mitochondrial gene gene human Homo sapiens Human foxo3_human Forkhead box protein O3 FOXO3 FKHRL1 FOXO3A Forkhead in rhabdomyosarcoma-like 1 AF6q21 protein protein protein human Homo sapiens Human MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG E5B4E830665A9982 sco2_human_gene SCO2_HUMAN_GENE gene gene human Homo sapiens Human sir3_human NAD-dependent protein deacetylase sirtuin-3, mitochondrial SIRT3 SIR2L3 SIR2-like protein 3 Regulatory protein SIR2 homolog 3 protein protein human Homo sapiens Human MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRDDVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK foxo3-sodm 765466 n/a 765468 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 765469 unspecified role unspecified role bait bait association association false false false 3D foxo3-human 765466 n/a 765470 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A-x 765469 unspecified role unspecified role bait bait association association false false false 3D sirt3a-foxo3 765467 n/a 765469 unspecified role unspecified role prey prey n/a 765471 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 physical association physical association false false false 1B and 1C A SIRT3 acetylation null mutant did also interact. The mutation site (his248tyr) could not be mapped to the SIRT3 aa sequence.