DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ li-2008-2 Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons. rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li H., Chen Y., Jones AF., Sanger RH., Collis LP., Flannery R., McNay EC., Yu T., Schwarzenbacher R., Bossy B., Bossy-Wetzel E., Bennett MV., Pypaert M., Hickman JA., Smith PJ., Hardwick JM., Jonas EA. Apoptosis - Interactions involving proteins with a function related to apoptosis Coimmunoprecipitations were carried out on lysates of rat hippocampal neurons. The authors confirmed by email that the BCL-XL construct used was human. mbennett@aecom.yu.edu,elizabeth.jonas@yale.edu Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2008 IMEx Only protein-protein interactions imex curation li-2008-1 Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons. rat-hippocampus Rattus norvegicus hippocampal neurons hippocampus Hippocampus [Brain hippocampus] anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Li H., Chen Y., Jones AF., Sanger RH., Collis LP., Flannery R., McNay EC., Yu T., Schwarzenbacher R., Bossy B., Bossy-Wetzel E., Bennett MV., Pypaert M., Hickman JA., Smith PJ., Hardwick JM., Jonas EA. Apoptosis - Interactions involving proteins with a function related to apoptosis Coimmunoprecipitations were carried out on lysates of rat hippocampal neurons. The authors confirmed by email that the BCL-XL construct used was human. mbennett@aecom.yu.edu,elizabeth.jonas@yale.edu Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2008 IMEx Only protein-protein interactions imex curation li-2008-3 Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons. in vitro In vitro gtpase assay gtpase assay GTPase gtp hydrolisis gtp hydrolisis GTPase predetermined featur predetermined feature Li H., Chen Y., Jones AF., Sanger RH., Collis LP., Flannery R., McNay EC., Yu T., Schwarzenbacher R., Bossy B., Bossy-Wetzel E., Bennett MV., Pypaert M., Hickman JA., Smith PJ., Hardwick JM., Jonas EA. Apoptosis - Interactions involving proteins with a function related to apoptosis Coimmunoprecipitations were carried out on lysates of rat hippocampal neurons. The authors confirmed by email that the BCL-XL construct used was human. mbennett@aecom.yu.edu,elizabeth.jonas@yale.edu Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2008 IMEx Only protein-protein interactions imex curation dnm1l_rat Dynamin-1-like protein Dnm1l Dlp1 Drp1 Dynamin-like protein protein protein rat Rattus norvegicus (Rat) Rat MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGVVTRRPLILQLVHVSPEDKRKTTGEENDPATWKNSRHLSKGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKVFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKSSKVPSALAPASQEPSPAASAEADGKLIQDNRRETKNVASAGGGIGDGGRIGDGGQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW p53563-1 Bcl-2-like protein 1 Bcl-xL Bcl2l1 Bclx Blc2l Apoptosis regulator Bcl-X protein protein rat Rattus norvegicus (Rat) Rat MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEPERETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK q07817-1 Bcl-2-like protein 1 BCLX Apoptosis regulator Bcl-X BCL2L1 BCL2L Bcl-xL protein protein human Homo sapiens Human MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 b2cl1_human Bcl-2-like protein 1 BCL2L1 BCL2L BCLX Apoptosis regulator Bcl-X protein protein human Homo sapiens Human MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK dnm1l_human Dynamin-1-like protein Dynamin-like protein Dnm1p/Vps1p-like protein Dynamin family member proline-rich carboxyl-terminal domain less Dynamin-related protein 1 Dynamin-like protein 4 Dynamin-like protein IV DNM1L DLP1 DRP1 protein protein human Homo sapiens Human MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGIVTRRPLILQLVHVSQEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKIFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKSSKVPSALAPASQEPSPAASAEADGKLIQDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW F9521A376B785B71 bcl2l1-dnm1l-1 Bcl2l1 (Bcl-xL) coimmunoprecipitates Dnm1l (Drp1) protein in rat brain lysate 733539 N/A 733542 unspecified role unspecified role prey prey N/A 733543 unspecified role unspecified role bait bait physical association physical association false false false 6 B bcl2l1-dnm1l-2 Bcl2l1 (Bcl-xL) coimmunoprecipitates with Dnm1l (Drp1) protein in rat brain lysate 733539 N/A 733542 unspecified role unspecified role bait bait N/A 733543 unspecified role unspecified role prey prey physical association physical association false false false 6 B bcl2l1-dnm1l-3 Human BCL2L1 (BCL-XL) coimmunoprecipitates endogenous Dnm1l (Drp1) protein in rat hippocampal neuron lysates 733540 N/A 733544 unspecified role unspecified role bait bait n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 733542 unspecified role unspecified role prey prey physical association physical association false false false 6 A Q07817-1 transduction dnml1-gtp BCL2L1 (BCL-XL) stimulates DNM1L (DRP1) GTPase activity 733541 N/A 733545 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 733546 stimulator stimulator unspecified role unspecified role N/A 733547 enzyme enzyme unspecified role unspecified role gtpase reaction gtpase reaction false false false 6 C Bcl-xL increased Drp1 GTPase activity by about 60%, relative to the control protein, BSA.