IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ xie-2007a-1 The 44 kD Pim-1 kinase phosphorylates BCRP/ABCG2 and thereby promotes its multimerization and drug resistant activity in human prostate cancer cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Xie Y., Xu K., Linn DE., Yang X., Guo Z., Shimelis H., Nakanishi T., Ross DD., Chen H., Fazli L., Gleave ME., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis yqiu@som.umaryland.edu A human HeLa cell cDNA library was used. J. Biol. Chem. (0021-9258) 2007 IMEx Cancer - Interactions investigated in the context of cancer Only protein-protein interactions imex curation imex curation xie-2007a-2 The 44 kD Pim-1 kinase phosphorylates BCRP/ABCG2 and thereby promotes its multimerization and drug resistant activity in human prostate cancer cells. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xie Y., Xu K., Linn DE., Yang X., Guo Z., Shimelis H., Nakanishi T., Ross DD., Chen H., Fazli L., Gleave ME., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis yqiu@som.umaryland.edu A human HeLa cell cDNA library was used. J. Biol. Chem. (0021-9258) 2007 IMEx Cancer - Interactions investigated in the context of cancer Only protein-protein interactions imex curation imex curation xie-2007a-3 The 44 kD Pim-1 kinase phosphorylates BCRP/ABCG2 and thereby promotes its multimerization and drug resistant activity in human prostate cancer cells. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Xie Y., Xu K., Linn DE., Yang X., Guo Z., Shimelis H., Nakanishi T., Ross DD., Chen H., Fazli L., Gleave ME., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis yqiu@som.umaryland.edu A human HeLa cell cDNA library was used. J. Biol. Chem. (0021-9258) 2007 IMEx Cancer - Interactions investigated in the context of cancer Only protein-protein interactions imex curation imex curation xie-2007a-6 The 44 kD Pim-1 kinase phosphorylates BCRP/ABCG2 and thereby promotes its multimerization and drug resistant activity in human prostate cancer cells. human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma confocal microscopy confocal microscopy immunostaining immunostaining Xie Y., Xu K., Linn DE., Yang X., Guo Z., Shimelis H., Nakanishi T., Ross DD., Chen H., Fazli L., Gleave ME., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis yqiu@som.umaryland.edu J. Biol. Chem. (0021-9258) 2007 IMEx Cancer - Interactions investigated in the context of cancer A human HeLa cell cDNA library was used. Only protein-protein interactions imex curation imex curation xie-2008-5 The 44-kDa Pim-1 kinase phosphorylates BCRP/ABCG2 and thereby promotes its multimerization and drug-resistant activity in human prostate cancer cells. human-cwr_r1 Homo sapiens prostate cancer cell line CWR-R1 cwr_r1 Human prostate cancer cell line CWR-R1 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Linn DE., Yang X., Guo Z., Shimelis H., Nakanishi T., Ross DD., Chen H., Fazli L., Gleave ME., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis yqiu@som.umaryland.edu A human HeLa cell cDNA library was used. J. Biol. Chem. (0021-9258) 2007 IMEx Cancer - Interactions investigated in the context of cancer Only protein-protein interactions imex curation imex curation p11309-2 Serine/threonine-protein kinase pim-1 Pim-1L PIM1 protein protein human Homo sapiens Human MPHEPHEPLTPPFSALPDPAGAPSRRQSRQRPQLSSDSPSAFRASRSHSRNATRSHSHSHSPRHSLRHSPGSGSCGSSSGHRPCADILEVGMLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK Initiates from CTG codon. abcg2_human Broad substrate specificity ATP-binding cassette transporter ABCG2 Placenta-specific ATP-binding cassette transporter Breast cancer resistance protein Mitoxantrone resistance-associated protein CDw338 ABCG2 ABCP BCRP BCRP1 MXR Urate exporter ATP-binding cassette sub-family G member 2 protein protein human Homo sapiens Human MSSSNVEVFIPVSQGNTNGFPATASNDLKAFTEGAVLSFHNICYRVKLKSGFLPCRKPVEKEILSNINGIMKPGLNAILGPTGGGKSSLLDVLAARKDPSGLSGDVLINGAPRPANFKCNSGYVVQDDVVMGTLTVRENLQFSAALRLATTMTNHEKNERINRVIQELGLDKVADSKVGTQFIRGVSGGERKRTSIGMELITDPSILFLDEPTTGLDSSTANAVLLLLKRMSKQGRTIIFSIHQPRYSIFKLFDSLTLLASGRLMFHGPAQEALGYFESAGYHCEAYNNPADFFLDIINGDSTAVALNREEDFKATEIIEPSKQDKPLIEKLAEIYVNSSFYKETKAELHQLSGGEKKKKITVFKEISYTTSFCHQLRWVSKRSFKNLLGNPQASIAQIIVTVVLGLVIGAIYFGLKNDSTGIQNRAGVLFFLTTNQCFSSVSAVELFVVEKKLFIHEYISGYYRVSSYFLGKLLSDLLPMRMLPSIIFTCIVYFMLGLKPKADAFFVMMFTLMMVAYSASSMALAIAAGQSVVSVATLLMTICFVFMMIFSGLLVNLTTIASWLSWLQYFSIPRYGFTALQHNEFLGQNFCPGLNATGNNPCNYATCTGEEYLVKQGIDLSPWGLWKNHVALACMIVIFLTIAYLKLLFLKKYS pim1-abcg2-3 Kinase dead Pim-1L interacts with BCRP in a yeast two-hybrid assay 684240 N/A 684245 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P11309-2:p.Lys158Met mutation mutation predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false N/A 684246 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false The PIM-1 protein used as the bait is a kinase inactive mutant. pim1-abcg2-1 FLAG-Pim-1L coimmunoprecipitates HA-BCRP in 293T lysates 684241 N/A 684245 unspecified role unspecified role bait bait n-terminal Region missing in Pim-1S required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 684246 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 1 A Q9UNQ0; P11309-1 transfection The data suggest that this interaction requires both the N-terminal proline-rich region and the kinase domain present in Pim-1L but not Pim-1S. pim1-abcg2-2 GST-PIM-1L pulls down BCRP from 293T lysates 684242 N/A 684246 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 684245 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 1 B The data suggest that this interaction requires both the N-terminal proline-rich region and the kinase domain present in Pim-1L but not Pim-1S. abcg2-3g-pim1-1 Pim-1L and BCRP colocalize in LNCaP cells 684243 N/A 684246 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 684245 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 1 C P11309-1; Q9UNQ0 transfection pim1-abcg2-9 Endogenous PIM-1L coimmunoprecipitates endogenous ABCG2 (BCRP) in CWR-R1 lysates 684244 N/A 684246 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 684245 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 1 D