IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ green-2007-2 Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis m.el-tanani@qub.ac.uk. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. Mol. Cancer Res. (1541-7786) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer green-2007-3 Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis m.el-tanani@qub.ac.uk. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. Mol. Cancer Res. (1541-7786) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer green-2007-4 Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis m.el-tanani@qub.ac.uk. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. Mol. Cancer Res. (1541-7786) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer green-2007-1 Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Green CD., Thompson PD., Johnston PG., El-Tanani MK. Apoptosis - Interactions involving proteins with a function related to apoptosis m.el-tanani@qub.ac.uk. A cDNA library constructed from EGF-stimulated MCF-7 human breast cancer cells was used. Mol. Cancer Res. (1541-7786) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV p20290-2 Transcription factor BTF3 BTF3b BTF3 NACB RNA polymerase B transcription factor 3 OK/SW-cl.8 Nascent polypeptide-associated complex subunit beta protein protein human Homo sapiens Human MKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNEAN p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD lt_sv40 Large T antigen protein protein sv40 sv40 SV40 MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET esr1-btf3-1 ERalpha A/B domain interacted with BTF3b in a directed yeast two-hybrid assay 680262 N/A 680266 unspecified role unspecified role bait bait n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 680267 unspecified role unspecified role prey prey physical association physical association false false false 2 A tp53-lt P53 interacts with the SV40 large T antigen in a directed yeast two-hybrid assay 680262 N/A 680268 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 680269 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2 A tp53-lt-1 P53 interacts with the SV40 large T antigen in a directed mammalian two-hybrid assay 680263 N/A 680269 unspecified role unspecified role prey prey N/A 680268 unspecified role unspecified role bait bait physical association physical association false false false 2 B esr1-btf3-2 ERalpha A/B domain interacted with BTF3b in a directed mammalian two-hybrid assay 680263 N/A 680266 unspecified role unspecified role bait bait n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 680267 unspecified role unspecified role prey prey physical association physical association false false false 2 B btf3-esr1 BTF3b coimmunoprecipitates ERalpha in MCF-7 lysates 680264 N/A 680267 unspecified role unspecified role bait bait N/A 680266 unspecified role unspecified role prey prey physical association physical association false false false 3 A P03372 In this experiment, coimmunoprecipitation of endogenous ERalpha when using BTF3 antibody was not observed. The authors suggest this is due to the association of unliganded ERalpha with HSPs, and the requirement for estradiol to dissociate this complex and stabilize the receptor. btf3-esr1-1 Endogenous BTF3b coimmunoprecipitates endogenous ERalpha in MCF-7 lysates 680264 N/A 680267 unspecified role unspecified role bait bait N/A 680266 unspecified role unspecified role prey prey physical association physical association false false false 3 C Estradiol - the association between ERa and BTF3 was significantly enhanced by the presence of estradiol. esr1-btf3 ERalpha interacted with BTF3b in a yeast two-hybrid assay 680265 N/A 680266 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 680267 unspecified role unspecified role prey prey physical association physical association false false false