IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ varfolomeev-2007-2 IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com Cell (0092-8674) 2007 Only protein-protein interactions 2 imex curation varfolomeev-2007-1 IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com Cell (0092-8674) 2007 Only protein-protein interactions 2 imex curation varfolomeev-2007-3 IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com Cell (0092-8674) 2007 Only protein-protein interactions 2 imex curation varfolomeev-2007-4 IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com Cell (0092-8674) 2007 Only protein-protein interactions 2 imex curation methyl 1-{(2s)-2-cyclohexyl-2-[(n-methyl-l-alanyl)amino]acetyl}-l-prolyl-beta-phenyl-l-phenylalaninate Monovalent IAP antagonist 1-{(2S)-2-cyclohexyl-2-[(N-methyl-L-alanyl)amino]acetyl}-L-prolyl-beta-phenyl-L-phenylalaninate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) birc2_human Baculoviral IAP repeat-containing protein 2 Inhibitor of apoptosis protein 2 TNFR2-TRAF-signaling complex protein 2 IAP homolog B RING finger protein 48 BIRC2 API1 MIHB RNF48 RING-type E3 ubiquitin transferase BIRC2 Cellular inhibitor of apoptosis 1 protein protein human Homo sapiens Human MHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDVKCFCCDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQGRYPHLLEQLLSTSDTTGEENADPPIIHFGPGESSSEDAVMMNTPVVKSALEMGFNRDLVKQTVQSKILTTGENYKTVNDIVSALLNAEDEKREEEKEKQAEEMASDDLSLIRKNRMALFQQLTCVLPILDNLLKANVINKQEHDIIKQKTQIPLQARELIDTILVKGNAAANIFKNCLKEIDSTLYKNLFVDKNMKYIPTEDVSGLSLEEQLRRLQEERTCKVCMDKEVSVVFIPCGHLVVCQECAPSLRKCPICRGIIKGTVRTFLS xiap_human E3 ubiquitin-protein ligase XIAP Inhibitor of apoptosis protein 3 X-linked inhibitor of apoptosis protein IAP-like protein XIAP API3 IAP3 BIRC4 Baculoviral IAP repeat-containing protein 4 RING-type E3 ubiquitin transferase XIAP protein protein human Homo sapiens Human MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS birc3_human Baculoviral IAP repeat-containing protein 3 Inhibitor of apoptosis protein 1 TNFR2-TRAF-signaling complex protein 1 IAP homolog C Apoptosis inhibitor 2 RING finger protein 49 BIRC3 API2 MIHC RNF49 RING-type E3 ubiquitin transferase BIRC3 Cellular inhibitor of apoptosis 2 protein protein human Homo sapiens Human MNIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRSSYHCAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGGKLSNWEPKDNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQLASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQVQASYPHLLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSRSLVKQTVQRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEKESNDLLLIRKNRMALFQHLTCVIPILDSLLTAGIINEQEHDVIKQKTQTSLQARELIDTILVKGNIAATVFRNSLQEAEAVLYEHLFVQQDIKYIPTEDVSDLPVEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS n,n'-(hexane-1,6-diyl)bis(1-{(2s)-2-cyclohexyl-2-[(n-methyl-l-alanyl)amino]acetyl}-l-prolyl-beta-phenyl-l-phenylalaninamide) Bivalent IAP antagonist N,N'-hexane-1,6-diylbis[1-{(2S)-2-cyclohexyl-2-[(N-methyl-L-alanyl)amino]acetyl}-L-prolyl-beta-phenyl-L-phenylalaninamide] small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) m3k14_human Mitogen-activated protein kinase kinase kinase 14 MAP3K14 NIK Serine/threonine-protein kinase NIK NF-kappa-beta-inducing kinase protein protein human Homo sapiens Human MAVMEMACPGAPGSAVGQQKELPKAKEKTPPLGKKQSSVYKLEAVEKSPVFCGKWEILNDVITKGTAKEGSEAGPAAISIIAQAECENSQEFSPTFSERIFIAGSKQYSQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHAGVALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQLCFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHPFPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPHLSKLACVDSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAHSLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETTGRAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPSSPERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQPVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP casp9_human Caspase-9 ICE-like apoptotic protease 6 Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 CASP9 MCH6 protein protein human Homo sapiens Human MDEADRRLLRRCRLRLVEELQVDQLWDALLSRELFRPHMIEDIQRAGSGSRRDQARQLIIDLETRGSQALPLFISCLEDTGQDMLASFLRTNRQAAKLSKPTLENLTPVVLRPEIRKPEVLRPETPRPVDIGSGGFGDVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS mv1-birc2-4 Binding of IAP antagonist MV1 to BIRC2 681992 N/A 681996 unspecified role unspecified role neutral component neutral component N/A 681997 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Supp. Table 2 Ki=1.45E-5M mv1-birc2-5 Binding of IAP antagonist MV1 to BIRC2 681992 N/A 681996 unspecified role unspecified role neutral component neutral component N/A 681997 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Supp. Table 2 Ki=4.0E-8M mv1-birc4-4 Binding of IAP antagonist MV1 to BIRC4 681992 N/A 681996 unspecified role unspecified role neutral component neutral component N/A 681998 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Supp. Table 2 Ki=4.83E-7M mv1-birc4-5 Binding of IAP antagonist MV1 to BIRC4 681992 N/A 681998 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 681996 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false Supp. Table 2 Ki=3.73E-7M mv1-birc3-1 Binding of IAP antagonist MV1 to BIRC3 681992 N/A 681999 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 681996 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false Supp. Table 2 Ki=8.41E-6M mv1-birc3-2 Binding of IAP antagonist MV1 to BIRC3 681992 N/A 681999 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 681996 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false Supp. Table 2 Ki=8.7E-8M bv6-birc2-2 Binding of IAP antagonist BV6 to BIRC2 681992 N/A 682000 unspecified role unspecified role neutral component neutral component N/A 681997 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Supp. Table 2 Ki=1.05E-5M bv6-birc2-3 Binding of IAP antagonist BV6 to BIRC2 681992 N/A 682000 unspecified role unspecified role neutral component neutral component N/A 681997 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Supp. Table 2 Ki=1.69-7M bv6-birc4-6 Binding of IAP antagonist BV6 to BIRC4 681992 N/A 682000 unspecified role unspecified role neutral component neutral component N/A 681998 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Supp. Table 2 Ki=9.05E-7M bv6-birc4-2 Binding of IAP antagonist BV6 to BIRC4 681992 N/A 682000 unspecified role unspecified role neutral component neutral component N/A 681998 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Supp. Table 2 Ki=4.86E-7M bv6-birc3-1 Binding of IAP antagonist BV6 to BIRC3 681992 N/A 682000 unspecified role unspecified role neutral component neutral component N/A 681999 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Supp. Table 2 Ki=2.69E-6M bv6-birc3-2 Binding of IAP antagonist BV6 to BIRC3 681992 N/A 682000 unspecified role unspecified role neutral component neutral component N/A 681999 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Supp. Table 2 Ki=2.34E-7M mv1-birc2-1 Binding of IAP antagonist MV1 to BIRC2 681993 N/A 681996 unspecified role unspecified role bait bait N/A 681997 unspecified role unspecified role prey prey bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment mv1-birc2-2 Binding of IAP antagonist MV1 to BIRC2 681993 N/A 681997 unspecified role unspecified role prey prey bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 681996 unspecified role unspecified role bait bait direct interaction direct interaction false false false Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment mv1-birc2-3 Binding of IAP antagonist MV1 to BIRC2 681993 N/A 681997 unspecified role unspecified role prey prey bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 681996 unspecified role unspecified role bait bait direct interaction direct interaction false false false Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment bv6-birc2-1 Binding of IAP antagonist MV1 to BIRC2 681993 N/A 682000 unspecified role unspecified role bait bait N/A 681997 unspecified role unspecified role prey prey bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Table 1, Supp. Table 1 The bivalent inhibitor binds at the second BIR site KD=2.9E-6M BV6 is biotinylated to enable attachment mv1-birc4-1 Binding of IAP antagonist MV1 to BIRC4 681993 N/A 681998 unspecified role unspecified role prey prey bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 681996 unspecified role unspecified role bait bait direct interaction direct interaction false false false Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment mv1-birc4-2 Binding of IAP antagonist MV1 to BIRC4 681993 N/A 681996 unspecified role unspecified role bait bait N/A 681998 unspecified role unspecified role prey prey bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment mv1-birc4-3 Binding of IAP antagonist MV1 to BIRC4 681993 N/A 681998 unspecified role unspecified role prey prey bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 681996 unspecified role unspecified role bait bait direct interaction direct interaction false false false Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment bv6-birc4-1 Binding of IAP antagonist BV6 to BIRC4 681993 N/A 682000 unspecified role unspecified role bait bait N/A 681998 unspecified role unspecified role prey prey bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction false false false Table 1, Supp. Table 1 BV6 is biotinylated to enable attachment The bivalent inhibitor BV6 binds to the second Bir region KD=4.3E-6M mv1-birc2-6 Binding of IAP antagonist MV1 to BIRC2 681994 N/A 681996 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 N/A 681997 unspecified role unspecified role neutral component neutral component bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 direct interaction direct interaction false false false Table 1, Supp Table 3 bv6-birc2-4 Binding of IAP antagonist MV1 to BIRC2 681994 N/A 681997 unspecified role unspecified role neutral component neutral component bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 2.0 N/A 682000 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 direct interaction direct interaction false false false Table 1, Table S3 mv1-birc4-6 Binding of IAP antagonist MV1 to BIRC4 681994 N/A 681996 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 N/A 681998 unspecified role unspecified role neutral component neutral component bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 direct interaction direct interaction false false false Table 1, Supp Fig. 3 bv6-birc4-7 Binding of IAP antagonist BV6 to BIRC4 681994 N/A 682000 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 N/A 681998 unspecified role unspecified role neutral component neutral component bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 direct interaction direct interaction false false false Table 1, Supp Table 3 birc2-map3k14-1 Interaction between BIRC2 (c-IAP1) and MAP3K14 (NIK) identified by coimmunoprecipitation 681995 N/A 682001 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 681997 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false bir1 region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 6B, Supp Fig. 7 Exact coordinates of BIRC2 required to bind MAP3$14 not given in text but is described as the region required for binding to Traf proteins which is Bir1. birc4-casp9-1 Interaction between BIRC4 and Caspase-9 demonstrated by coimmunoprecipitation 681995 N/A 682002 unspecified role unspecified role prey prey p35 region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined certain certain sequence position certain false N/A 681998 unspecified role unspecified role bait bait bir3 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false IAP antagonists such as MV1 and BV6 Sup Fig. S2 P98170 transfection; P55211 transfection;