UniProt UniProt http://www.uniprot.org kim-2007f-5 Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Kim E.J., Kho J.H., Kang M.R., Um S.J. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SIRT1 expression plasmids provided by E.S. Hwang according pmid:18230337 and fig 2 (seq length) ejk93@hanmail.net Mol. Cell (1097-2765) 2007 mimix curation kim-2007f-1 Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kim E.J., Kho J.H., Kang M.R., Um S.J. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SIRT1 expression plasmids provided by E.S. Hwang according pmid:18230337 and fig 2 (seq length) ejk93@hanmail.net Mol. Cell (1097-2765) 2007 mimix curation kim-2007f-2 Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim E.J., Kho J.H., Kang M.R., Um S.J. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SIRT1 expression plasmids provided by E.S. Hwang according pmid:18230337 and fig 2 (seq length) ejk93@hanmail.net Mol. Cell (1097-2765) 2007 mimix curation kim-2007f-3 Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. imaging technique imaging technique microscopy immunostaining immunostaining Kim E.J., Kho J.H., Kang M.R., Um S.J. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SIRT1 expression plasmids provided by E.S. Hwang according pmid:18230337 and fig 2 (seq length) ejk93@hanmail.net Mol. Cell (1097-2765) 2007 mimix curation kim-2007f-4 Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity. in vitro In vitro deacetylase assay deacetylase assay predetermined predetermined participant predetermined Kim E.J., Kho J.H., Kang M.R., Um S.J. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SIRT1 expression plasmids provided by E.S. Hwang according pmid:18230337 and fig 2 (seq length) ejk93@hanmail.net Mol. Cell (1097-2765) 2007 mimix curation kim-2007f-6 Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kim E.J., Kho J.H., Kang M.R., Um S.J. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SIRT1 expression plasmids provided by E.S. Hwang according pmid:18230337 and fig 2 (seq length) ejk93@hanmail.net Mol. Cell (1097-2765) 2007 mimix curation kim-2007f-7 Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim E.J., Kho J.H., Kang M.R., Um S.J. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SIRT1 expression plasmids provided by E.S. Hwang according pmid:18230337 and fig 2 (seq length) ejk93@hanmail.net Mol. Cell (1097-2765) 2007 mimix curation kim-2007f-8 Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim E.J., Kho J.H., Kang M.R., Um S.J. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SIRT1 expression plasmids provided by E.S. Hwang according pmid:18230337 and fig 2 (seq length) ejk93@hanmail.net Mol. Cell (1097-2765) 2007 mimix curation kim-2007f-9 Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim E.J., Kho J.H., Kang M.R., Um S.J. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SIRT1 expression plasmids provided by E.S. Hwang according pmid:18230337 and fig 2 (seq length) ejk93@hanmail.net Mol. Cell (1097-2765) 2007 mimix curation kim-2007f-10 Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Kim E.J., Kho J.H., Kang M.R., Um S.J. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SIRT1 expression plasmids provided by E.S. Hwang according pmid:18230337 and fig 2 (seq length) ejk93@hanmail.net Mol. Cell (1097-2765) 2007 mimix curation aros_human Active regulator of SIRT1 RPS19BP1 AROS 40S ribosomal protein S19-binding protein 1 protein protein human Homo sapiens Human MSAALLRRGLELLAASEAPRDPPGQAKPRGAPVKRPRKTKAIQAQKLRNSAKGKVPKSALDEYRKRECRDHLRVNLKFLTRTRSTVAESVSQQILRQNRGRKACDRPVAKTKKKKAEGTVFTEEDFQKFQQEYFGS sir1_human NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 SIRT1 SIR2L1 SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein human Homo sapiens Human MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD rps19bp1-sirt1-3 1189842 n/a 1189852 unspecified role unspecified role bait bait n/a 1189853 unspecified role unspecified role prey prey physical association physical association false false false S5 sirt1-rps19bp1-3 1189842 n/a 1189852 unspecified role unspecified role prey prey n/a 1189853 unspecified role unspecified role bait bait physical association physical association false false false 2D sirt1-rps19bp1 1189843 n/a 1189853 unspecified role unspecified role bait bait n/a 1189852 unspecified role unspecified role prey prey physical association physical association false false false no figure sirt1-rps19bp1-1 1189844 n/a 1189852 unspecified role unspecified role prey prey n/a 1189853 unspecified role unspecified role bait bait physical association physical association false false false 1B rps19bp1-sirt1 1189845 n/a 1189853 unspecified role unspecified role neutral component neutral component n/a 1189852 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S2 rps19bp1-sirt1-2 1189846 n/a 1189854 enzyme target enzyme target substrate substrate neutral component neutral component n/a 1189853 enzyme enzyme neutral component neutral component direct interaction direct interaction false false false S4 Deacetylation is enhanced by RPS19BP1/AROS sirt1-rps19bp1-2 1189847 n/a 1189852 unspecified role unspecified role prey prey n/a 1189853 unspecified role unspecified role bait bait direct interaction direct interaction false false false 2C rps19bp1-sirt1-4 1189848 n/a 1189853 unspecified role unspecified role prey prey n/a 1189852 unspecified role unspecified role bait bait physical association physical association false false false 2D rps19bp1-sirt1-5 1189849 n/a 1189852 unspecified role unspecified role bait bait n/a 1189853 unspecified role unspecified role prey prey physical association physical association false false false 2E sirt1-rps19bp1-4 1189850 n/a 1189853 unspecified role unspecified role bait bait n/a 1189852 unspecified role unspecified role prey prey physical association physical association false false false 6A tp53-rps19bp1 1189851 n/a 1189853 unspecified role unspecified role prey prey n/a 1189854 unspecified role unspecified role bait bait n/a 1189852 unspecified role unspecified role prey prey association association false false false S6