IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ott-2007-1 The mitochondrial TOM complex is required for tBid/Bax-induced cytochrome c release. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ott M., Norberg E., Walter KM., Schreiner P., Kemper C., Rapaport D., Zhivotovsky B., Orrenius S. Apoptosis - Interactions involving proteins with a function related to apoptosis The N. crassa TOM core complex (Tom40, Tom22, Tom5, Tom6 and Tom7) was incubated with tBID and BAX for 15 min at 30 C, followed by immunoprecipitation. Sten.Orrenius@ki.se J. Biol. Chem. (0021-9258) 2007 ott-2007-2 The mitochondrial TOM complex is required for tBid/Bax-induced cytochrome c release. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ott M., Norberg E., Walter KM., Schreiner P., Kemper C., Rapaport D., Zhivotovsky B., Orrenius S. Apoptosis - Interactions involving proteins with a function related to apoptosis The N. crassa TOM core complex (Tom40, Tom22, Tom5, Tom6 and Tom7) was incubated with tBID and BAX for 15 min at 30 C, followed by immunoprecipitation. Sten.Orrenius@ki.se J. Biol. Chem. (0021-9258) 2007 tom40_neucr Mitochondrial import receptor subunit tom40 tom40 Protein MOM38 Translocase of outer membrane 40 kDa subunit mom38 B10H4.150 NCU01179 protein protein neucr Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) MASFSTESPLAMLRDNAIYSSLSDAFNAFQERRKQFGLSNPGTIETIAREVQRDTLLTNYMFSGLRADVTKAFSLAPLFQVSHQFAMGERLNPYAFAALYGTNQIFAQGNLDNEGALSTRFNYRWGDRTITKTQFSIGGGQDMAQFEHEHLGDDFSASLKAINPSFLDGGLTGIFVGDYLQAVTPRLGLGLQAVWQRQGLTQGPDTAISYFARYKAGDWVASAQLQAQGALNTSFWKKLTDRVQAGVDMTLSVAPSQSMMGGLTKEGITTFGAKYDFRMSTFRAQIDSKGKLSCLLEKRLGAAPVTLTFAADVDHVTQQAKLGMSVSIEASDVDLQEQQEGAQSLNIPF bax_human Apoptosis regulator BAX BAX BCL2L4 Bcl-2-like protein 4 protein protein human Homo sapiens Human MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG tom40_yeast Mitochondrial import receptor subunit TOM40 TOM40 ISP42 YMR203W YM8325.04 MOM38 Mitochondrial import site protein ISP42 Translocase of outer membrane 40 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAPTPLAEASQIPTIPALSPLTAKQSKGNFFSSNPISSFVVDTYKQLHSHRQSLELVNPGTVENLNKEVSRDVFLSQYFFTGLRADLNKAFSMNPAFQTSHTFSIGSQALPKYAFSALFANDNLFAQGNIDNDLSVSGRLNYGWDKKNISKVNLQISDGQPTMCQLEQDYQASDFSVNVKTLNPSFSEKGEFTGVAVASFLQSVTPQLALGLETLYSRTDGSAPGDAGVSYLTRYVSKKQDWIFSGQLQANGALIASLWRKVAQNVEAGIETTLQAGMVPITDPLMGTPIGIQPTVEGSTTIGAKYEYRQSVYRGTLDSNGKVACFLERKVLPTLSVLFCGEIDHFKNDTKIGCGLQFETAGNQELLMLQQGLDADGNPLQALPQL tom22_yeast Mitochondrial import receptor subunit TOM22 MOM22 TOM22 MAS17 MAS22 YNL131W N1217 N1862 Mitochondrial 22 kDa outer membrane protein Translocase of outer membrane 22 kDa subunit Protein MAS17 Mitochondrial 17 kDa assembly protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MVELTEIKDDVVQLDEPQFSRNQAIVEEKASATNNDVVDDEDDSDSDFEDEFDENETLLDRIVALKDIVPPGKRQTISNFFGFTSSFVRNAFTKSGNLAWTLTTTALLLGVPLSLSILAEQQLIEMEKTFDLQSDANNILAQGEKDAAATAN 6ACEC5D37D143CD3 tom40-bax TOM40 coimmunoprecipitates BAX in vitro 745405 N/A 745407 unspecified role unspecified role bait bait N/A 745408 unspecified role unspecified role prey prey physical association physical association false false false 3 A bax-tom40 TOM40 coimmunoprecipitates with BAX in vitro 745405 N/A 745408 unspecified role unspecified role bait bait N/A 745407 unspecified role unspecified role prey prey physical association physical association false false false 3 A tom22-bax Tom22 coimmunoprecipitates Tom40 and BAX in vitro 745406 N/A 745409 unspecified role unspecified role prey prey N/A 745410 unspecified role unspecified role bait bait N/A 745408 unspecified role unspecified role prey prey association association false false false 3 B BAX was coimmunoprecipitated in lysates from mitochondria exposed to BAX for 2 min but not after 30 min. The authors suggest this is because BAX interacts transiently with the TOM complex during its insertion into the outer mitochondrial membrane. tom22-tom40 Tom22 coimmunoprecipitates Tom40 in vitro 745406 N/A 745410 unspecified role unspecified role bait bait N/A 745409 unspecified role unspecified role prey prey physical association physical association false false false 3 B BAX was coimmunoprecipitated in lysates from mitochondria exposed to BAX for 2 min but not after 30 min. The authors suggest this is because BAX interacts transiently with the TOM complex during its insertion into the outer mitochondrial membrane.