UniProt UniProt http://www.uniprot.org starostina-2007-2 A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu Dev. Cell (1534-5807) 2007 Only protein-protein interactions imex curation starostina-2007-3 A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu Dev. Cell (1534-5807) 2007 Only protein-protein interactions imex curation starostina-2007-1 A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu Dev. Cell (1534-5807) 2007 Only protein-protein interactions imex curation starostina-2007-4 A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu Dev. Cell (1534-5807) 2007 Only protein-protein interactions imex curation fem1_caeel Sex-determining protein fem-1 fem-1 isx-1 Feminization of XX and XO animals protein 1 F35D6.1 protein protein caeel Caenorhabditis elegans MTPNGHHFRTVIYNAAAVGNLQRIKVFTINSRNDRQWIIDCFNSDQDGRYPLVIAARNGHANVVEYLLEIGADPSVRGVVEFDNENIQGTPPLWAASAAGHIEIVKLLIEKANADVNQATNTRSTPLRGACYDGHLDIVKYLLEKGADPHIPNRHGHTCLMIASYRNKVGIVEELLKTGIDVNKKTERGNTALHDAAESGNVEVVKILLKHGSVLMKDIQGVDPLMGAALSGFLDVLNVLADQMPSGIHKRDALKLLGSTLLDKKMDAMSAMNCWKQSMEVPLHADDLRLVREMETFFEPQEVYEYQREAQNIAQVELLDGNIEAQRMQALVIRERILGGAHTDVHYYLRFRGAVYCDMGQMNRCYDLWKHALELQQKHFAPLYYGTITTLQSFHETFSMSLNDFVNNHHANRNLRVRSSWVKYVFNGVCLELERAAAWTGAPLLEDTECCGKERCQHATEESEYKKLVYVAVHLVNVLERLSLPSAHIDDSDEEKEPKADVRRLMIVCHELHIPLLHHTLEERIPDSNSAELGLPKAAVLEQLLELDLDVNATDKNNDTPMHILLRAREFRKSLVRALLVRGTWLFARNRHGDVVLNVMKRMKALNHANFDDLPLGRHITLAGLVANAMRVKYPKKFVGVEEQMPLELRRFYLAH q9bks1_caeel Elongin-C elc-1 Y82E9BR.15 CELE_Y82E9BR.15 protein protein caeel Caenorhabditis elegans MADQNNAIQCDQDAAQPKQYGGIEGPTSQYVKLVSSDDHEFIIKRELALTSGTIRAMLSGPGVYAENESNVVYFREIPSHVLQKVCQYFAYKVRYTHAATEIPEFPIPPDVALELLMAANFLDC p34708-1 Sex-determining transformer protein 1 Tra-1L tra-1 her-2 Hermaphrodization of XO animals protein 2 Y47D3A.6 protein protein caeel Caenorhabditis elegans MMAPSTEDPDTVVEAQRRGSFSKKKNANGWNKVELVDQCAKQMGSEDKQPGGGDVKTENDPSKNGLGSATSNFIQSSVPPSHQTLSNPLQLSPPAEASVAQQSGASQVFPTFQAALGASSDELLQPNATSSSTSSSASTSSIVPVVKFTNQTAPNGSTVATSVGQNVRLTINGKRVGRPPGTFKRPQNNAANSSNSGNDSDMMGDHDLTCRWKSCNSSFQTLKALVDHVQESHVQSTEQEHHAWRCEWEGCDRNETFKALYMLIVHVRRHTGEKPNKCEYPGCGKEYSRLENLKTHRRTHTGEKPYKCEFADCEKAFSNASDRAKHQNRTHSNLKPYSCQIPQCTKSYTDPSSLRKHIKAVHGDDEYEKAKKSRPANYSNRRRPDHRLAPPTGAMSHPYLATPNSGASVVAHSSVHQQNFINMALAQHHHNAQRAQQLMAATGNVMPMMDPASAAAAAQAQAHHQAQAQMLQTHMMQQAQIQAAAQMQAQVQHQAAMQAHAMQQAQMVLQNNLLGAQSLLSPFSPLLPPSRAPNVMAMLQTPPTPTSVAPMFDIMTSRAPMAPVVSAPTAPAPLVPAPVPASPVFDELREQMREVEPLQQQQQQEPMDQDLQDIRVDGDSDDEDEEEPRTPSGALLLPRGGNNGDGGFGGSGSSRASSGSGTMELSAAPISQNGSRASGSGERGMRSFLIADILQLAADFQNERLLSDVLDLAIFDTRDVRSLHNIYQVYIRAHKAIPITRRPLDWNETHQLHNLYHDPRFNRAEHQDSPAIRDRDTRFWRTIAEANTMRQRQIEPVPLDDDDEGYFDEMVHRVQNGRLNEQFMEGFESDDDDGFEDEDDVPGLGIAVYRGRRRVRREALKQANLDIQEAETAGRNVGGFGDEEDRNNRGHDQDRSFLDHYYPPMVVVVETESPQIVRDQEMMRQFEEAKKNVETDEIKKRAEAMQFGTSSSHHHTKTLLIQRALFDKTSSVRRSLLQFITISVDQEELRQSCHATSAPQGAHVVHNVVDEFDSIMRAQEDSNNRILLSLDIPAPSAVTGVSGSITHADNSALQLQQEQPTSSFSSWFPEDDPIYALPPPPPPPAPPRRRRSADNKDDSENIPKKPRHQF cul2_human Cullin-2 CUL2 protein protein human Homo sapiens Human MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA fem2_caeel Protein phosphatase fem-2 Ca(2+)/calmodulin-dependent protein kinase phosphatase fem-2 Sex-determining protein fem-2 Feminization of XX and XO animals protein 2 T19C3.8 protein protein caeel Caenorhabditis elegans MEKVNEERDAVFEDHIGDRRRSVRSLLEEAFADEMEKTSYDVEVADTPQPHIPIRFRHPPIAGPVHDVFGDAIHDIFQKMMKRGQAVDFCHWVSHLIATEIDEKFSEVAFRDVQYNPDIYVTDSTTEAKKLFNDKIWPAIDKILQQNAETCPILSEKWSGIHVSGDQLKGQRHKQEDRFLAYPNGQYMDRGEDPISVLAVFDGHGGHECSQYAAGHLWETWLEVRKSRDPSDSLEDQLRKSLELLDERMTVRSVKECWKGGSTAVCCAIDMDQKLMALAWLGDSPGYVMSNIEFRQLTRGHSPSDEREARRVEEAGGQLFVIGGELRVNGVLNLTRALGDVPGRPMISNEPETCQVPIESSDYLVLLACDGISDVFNERDLYQLVEAFANDYPVEDYAELSRFICTKAIEAGSADNVSVVIGFLRPPQDVWKLMKHESDDEDSDVTDEE fem3_caeel Sex-determination protein fem-3 Feminization of XX and XO animals protein 3 Ce-FEM-3 C01F6.4 fem-3 protein protein caeel Caenorhabditis elegans MEVDPGSDDVEADRETRAQKLKLKRNVKFRAQMRRFDEYCGVTNLTVDDLNWPLISGIPLQRQRLTGATYYDDSLLDQNPWDEFSIDRFLEITSIQLITAGAGYERNDEITRFVFQRTMKTIVTYCNFMYDLARRNGKVQITRFELQDLIHRDEFRFYMYFRQFLPNPDPNCTAFSNHYTSLLHTLYFNIPGMPQFWNNSQMYNYAATRGQRLVQNIAAFYPPEYFWNEDESKYHTTFVVPRGTEFSKFYARRFHEALGMPPLENEIITVLDWLAKLCILEIVYHTTIWCDITGFGGLPRIEHYRLAMENVEDIIFDLAIDDFSISRLQLQISPFEISRYSPLDVSGYYETIKRKKDIEEYQNRFYEVHYSDDVRIMNVYATDCSRKR vhl_caeel von Hippel-Lindau tumor suppressor homolog vhl-1 F08G12.4 protein protein caeel Caenorhabditis elegans MSDGSMDDDGRLFPDLGSSTHDNREIRVRFLNRCAYPVDVFWLNPSKQPTKYGTLAQKKYLDIKTFKDHPWVARRSFDGCKVLVNEKEVFWPEPAPRMNLIVRNHCVITMKVQSLREIAGRSFLRHNPTEVPNKIKGLPRELQFEVKHFLDRKQEYSEIVCRSIPPPGPQRPQQ zer1_caeel Zyg eleven-related protein 1 zer-1 zyg-11-related protein T24C4.6 protein protein caeel Caenorhabditis elegans MSHTADIPLEAPTLLKLASSSVQSQINNDEYMETVILPVPLSQELFARFRNKWNIHNHGPAPVEHLGPPTDEPRPDQGNIDMIKQVFRIWSDSTRLPLEKLNLTGATVDDQIFVDLLHAHAHSITELDLTNVTGVTDLSNAYLYARSTHFPMLTSIRMTSMDLVTGHQPRRKNGIASTEFRALFMLGEEALAEARGQMERDGLRSPLSPSSQPSSIQSDHQSLDALVPCDSPVEPPLITARAPNVKRLFLPRCPAKRTTIDEEEQNTHVILTKILSPLQQLEVLDLSYWSKTDDMRCLQPLSNTLTCLILYDVPDLYHAVPNICHMTELRILDVSQSNRDSGLYPHPVTTLNKLIVSLKYLTHLDISSTNLATQPSSHDNPARYRESVRTDICGLQSLVRPLKYLGLFNCESASHVREIPAEVVSGDANEDQVITSLQMYKDRAGLLQNVLNESYQLYRFGNSNPLTRHTEALHLVLEAMHRHLADSTLQIAGSASLFYIIRKVDMNRDTKRRVVSALLSGMEVHMEEQVMVRNCCLSLCQFEIPQDILFDYSRLAVLLVSVLQHHNADNLTQRIVVFLLNSMACHVEGDQKVQVGSYGAIEMILDQIARKHTANVCDDVMEVGWSFLWNITDETPVNCELFLNANGLDLFQKCYEAFKTERELVRNMMGLIGNIAEVDELRSQLMKDDYVKIFCALLESQEESIEISYNSAGVLAHMVSDGEEVWKQMTVCRNEVMQRIVEATSSWKLATRRFINYRSFRPILRLLPLYHAYASQHWAVWALANLTTTDGEKYCAYVRDEGGVPLLEELVSNAITTTDIRTLANTVLENIRNVENKRNPKSIPADLAHSSNSPTFVPSYVSDVEEEDEGDVEADEEVQDFVNVDFDDYALPMEY cand1_caeel Cullin-associated NEDD8-dissociated protein 1 cand-1 Cullin-associated and neddylation-dissociated protein 1 Y102A5A.1 protein protein caeel Caenorhabditis elegans MSAYHVGQLVDKMSNPDKDFRFMACNDLMKDLQTGTIALEDDSTAKVIRALIKLLSDSNGEVQNLAIKCIGLLAQPSKIKTHHLEYLVEELTPHVFSKAEQSRDIHSLTLKAMILNLAPSASSNATTTVVKRMLPKFVDSLSLCAPDDAARVDVLDLIGEVLLRFGDVVPEMHKGSLKVMVDHLYSFRSAIRKKAITGIGHLASVINGELYDELVQDLLKELAQRSPPSSAAQNVQLRTLVIALSTVARASGSRFSKHTPKVVPFLLQYLQIDPGTESEHDDLREASIQGLEVFLYRNPQEVVAFEKEVIQQLTDALAYDPNYEYGDDDEDEQMEDDEDDDEDEYSDDEDVTWKVRRAAAKAIEAMISSHRESLLNLSQKIGPVVIGRFKEREETVRTEIISVYIALLNQISILVPDLQKAVVAADEDSIETDDIVVIGGTKFSTNYLSRSQLAIIQSLADQKDVLLRTITKSMKKHPKTGPKCIELLSALIRTYPSGLEDSLDDIIPAVSNILTDKNASAQGKMTVLSFISNALTLNNPKRFKNLLSPLTTIMTHSISEPFYKVSAEGLAVCCKYIDVLRELSACGGNEEAKKLLVVVEKKFMANDTDQEVRERAISAISMLLAAFKDVLKNETPAILEKMTERIGRDMTCLVAFRASTHIVEAGIIFSSAQLQSILRHVVDYVKKIARSLRMTCLNFVEKLMKHSPAGSIPVEELTCVLGEMSNLISETDLQITNQAFCCLTYAFLNFPTCVSLHMQPILDSIIRLLTSPLIQGLALNSLLNLFTAIVKTDFPEKPTFESLLDSVTSPVYDNVALSRHAHMAIASCAAVITESTQNLEKSRSLAKKLAQQLQTANMSDSIRLFAMITLGELGRRVPDTYSPDFPVKPEDLAIKAFNHHHEDLKSAAAQALGALAVGNLNVYLPFILEQIRTQPKKQYLLLHALKEVIVWESSSEESTKSTDLFRSAIVDIWGMLMANAGGNEDGTRSVVAECLGRLCSFDPESLLPKLKESMRSSDPAIRSSAVSAIKYMINDEKRIVDITLQKQIGDFLAAVRDEDLKVRRVALVVLNSAAHNKPALVRDLLPDLLPAVYEETKLRKELIKEVEMGPFKHQVDEGLDLRKCAFECMFTLLESCVDKIDITQFSSVMEVGLSDQNHDVKLLNYLTLQRVANLAPGQVLQRIDRVCEPLKTQLNVRPRGNAVKQEVEKLEELKKAVIRVVYGLKLKLPEVERNPQFLDLYNTIKHTKELEALANDVLKESQRAVVYDTPMETA rbx1_caeel RING-box protein 1 rbx-1 Ce-rbx-1 ZK287.5 protein protein caeel Caenorhabditis elegans MAQASDSTAMEVEEATNQTVKKRFEVKKWSAVALWAWDIQVDNCAICRNHIMDLCIECQANQAAGLKDECTVAWGNCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH cul2_caeel Cullin-2 cul-2 ccd-3 div-3 ZK520.4 protein protein caeel Caenorhabditis elegans MASSTKVSLRRPPRPQVMYSLKPKVVEFDKVWVQLRPSIIDIINLRPITNVQWHHKFSDVYDICVSIPTPLSERLYNEVKACIQEHVRQKRQDIVDVDPDLLLQEYHKMWRVFHEGAIFIHRLFGYLNKQFVKQKRCTDLDNFAQYAAFLQIPDVKEIGCLALEIWKEDLVKTILPQLVKLLLIAIDNDRKGNFPHIANEVSGVINSFVKMEETDFDVVPAEGARYKARESTTAFYQESFEKPLLTDTEQYYSALAQKMLTDLSCSEYMEQVIVLLEQEEMRAKKYLHESSVEKVITLCQKVMIKAHKDKLHAVCHDLITNEENKDLRNMYRLLKPIQAGLSVMVKEFEEYVKKKGLEAVSRLTGENVPQQFVENVLRVYNKFNDMKTAVFMDDGEFSSGLDKALQGVVNSKEPGQSVPKASERLARYTDGLLKKSTKGLSETDLEAKLDSAIVIFRYIEDKDIFQKFYSKMLANRLIASTSISMDAEELMINKLKQACGYEFTSKLSRMFTDIGLSQELSNNFDKHIADIKTVQPDVKFVPTQTMILQAGSWPLNAPQLSTNSNNQTAQDVANFHLPRILQPVIQEFEKFYTGKHNGRKLTWLFNMSQGDVRLTYLDKQYVAQMYVYQMAALLCFERRDAILVKDIGEEIGVSGDYLLKTIRTILDVTLLTCDDQNLTADSLVRLNMSMTSKRMKFRLQAPQVNKAVEKEQEAVANTVSQDRKYYMECAIVRIMKTRKVLKHNALVTEIMDQTKGRFSPDVPFIKKSIEDLIEKMYIQRTDQNDEYQYLA zyg11_caeel Early embryogenesis protein zyg-11 zyg-11 C08B11.1 protein protein caeel Caenorhabditis elegans MDHNAALTTCSTSAIPRLARLATERIAELIENDALPEFDHKVIASCSNDIFEVLRQKKKLNHQTLQTMCSTSRFQINKIDLMGNKVELQDLELCSNQNLVSFRLGDIDYFPNTNKIQVADVLDTALNRTSRQQLRHLDLSGRHRITSNWPTYVARKLPRLESLAFANRSTANETLSQIGASLLNLRFLDISSTCVTDISCISSLRNLEVLIMYNLNILKGDVTETLSNLTKLRVLDISRKVNTDYLQETSQDAHLDLALGIYNRSVEAIESGTATPWAELRAIDMSGLSIVQFGTDRALAFVEKIIEAHPKLEQISLLATPLDSSLIEIPNRNLQVINTVSRRSIIFALSHYANLDRPAFITHALHSVYYQLQSGYDKFSQEELKECLRLVCISMQQGLNTLPVQIAGSACLYHLCKMKRIKRLSVKEVNNCIERSLDAAEQYRSMTQLQKNVWLTICNDYLLHLDEIDFYRTCKVALDTMLLNRDASVERMTIAIVSIVTPKMRPSEAKILTTETKYVYHLVKIMNDYLEAYTREHRVGHERDNENALYTLKFTLSALWNLTDECPATCKAFLDAGGVQIAFRILKAFDYHGNVQTKVLGILNNLAEVEELHLGQLCKNEYISVLISCLDGSFNEVDSKGRYREVERSYFAAGILANLLTNTDGWESENQRDEACEKLLELIEQYPTLPSAMVSYKSFIPFSRIVRESNSNGAIMWCLWGVHHVLQHREKNKAPTYEKGYIEMFMDSGLSPIVEDMSHGHSKHIHNLDIRVVHLAREIIDIVSCHSLSSSPVRLVRRV g5ecr7_caeel 3L686 elb-1 Y41C4A.10 CELE_Y41C4A.10 protein protein caeel Caenorhabditis elegans MSQQELFFEVRRNRSHIYLDAEESHSVMELKLMLAGITRDPVNHMELWKLDEDGNKSQLLHDTATLSDAGFSSTNAKAQCPAALGLRLTNAEEHLTISDVSTAPPIPDTMRQEQAPQE fem1-elc1-1 768511 n/a 768515 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region binding region binding-associated region binding region mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false n/a 768516 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 1C fem1-tra1a-1 768511 n/a 768515 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768517 unspecified role unspecified role prey prey n-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 5A, 6A Interaction is observed only in the presence of the proteasome inhibitor N-acetyl-L-leucinyl- L-leucinal-L-norleucinal (LLnL). fem1-cul2-1 768511 n/a 768515 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768518 unspecified role unspecified role prey prey physical association physical association false false false Fig 5B fem2-tra1a-1 768511 n/a 768519 unspecified role unspecified role bait bait n-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768517 unspecified role unspecified role prey prey n-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 6A Interaction is observed only in the presence of the proteasome inhibitor N-acetyl-L-leucinyl- L-leucinal-L-norleucinal (LLnL). fem3-tra1a-1 768511 n/a 768517 unspecified role unspecified role prey prey n-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768520 unspecified role unspecified role bait bait n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 6A Interaction is observed only in the presence of the proteasome inhibitor N-acetyl-L-leucinyl- L-leucinal-L-norleucinal (LLnL). fem1-fem2-1 768511 n/a 768519 unspecified role unspecified role prey prey n-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768515 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768520 unspecified role unspecified role prey prey n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 6D fem2-fem1-2 768511 n/a 768519 unspecified role unspecified role bait bait n-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768515 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768520 unspecified role unspecified role prey prey n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 6D fem3-fem1-2 768511 n/a 768519 unspecified role unspecified role prey prey n-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768515 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768520 unspecified role unspecified role bait bait n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 6D elc1-fem1-2 768511 n/a 768519 unspecified role unspecified role prey prey n-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768515 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768518 unspecified role unspecified role prey prey n/a 768517 unspecified role unspecified role prey prey n/a 768520 unspecified role unspecified role prey prey n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 768516 unspecified role unspecified role bait bait n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 6E, 7B elc1-fem1-1 768512 n/a 768515 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 mutation analysis mutation analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 768516 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Fig 1D fem2-fem1-1 768512 n/a 768519 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) n/a 768515 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 mutation analysis mutation analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig 6B fem2-tra1a-2 768512 n/a 768519 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) n/a 768517 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig 6B fem3-tra1a-2 768512 n/a 768520 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) n/a 768517 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig 6B fem3-fem1-1 768512 n/a 768515 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 768520 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Fig 6B tra1a-fem1-1 768512 n/a 768515 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 768517 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Fig 6C cul2-elc1-1 768513 n/a 768521 unspecified role unspecified role prey prey n/a 768522 unspecified role unspecified role prey prey n/a 768523 unspecified role unspecified role prey prey n/a 768524 unspecified role unspecified role prey prey n/a 768515 unspecified role unspecified role prey prey n/a 768525 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 768526 unspecified role unspecified role prey prey n/a 768527 unspecified role unspecified role prey prey n/a 768516 unspecified role unspecified role prey prey physical association physical association false false false Fig 1A cul2-tra1a-1 768514 n/a 768525 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 768517 unspecified role unspecified role prey prey physical association physical association false false false Fig 5C Interaction is observed only when pbs-4 RNAi is used to inactivate the proteasome.