UniProt UniProt http://www.uniprot.org george-2007-4 Dimerization and actin-bundling properties of villin and its role in the assembly of epithelial cell brush borders. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column ms participant Identification by mass spectrometry ms participant George SP., Wang Y., Mathew S., Srinivasan K., Khurana S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cross-linking agents Dithiobis (succinimidylpropionate) (DSP); Disuccinimidyl suberate (DSS); Dimethyl 3,3′-dithiobispropionimidate (DTBP); Ethylene glycol bis (succinimidylsyccinate) (EGS); 1,5-Difluoro-2,4-dinitrobenzene (DFDNB); and 3,3′ Dithiobis (sulfosuccinimidyl propionate) (DTSSP). skhurana@utmem.edu J. Biol. Chem. (0021-9258) 2007 mimix curation george-2007-1 Dimerization and actin-bundling properties of villin and its role in the assembly of epithelial cell brush borders. in vitro In vitro crosslink cross-linking study crosslink weight identificat confirmation by molecular weight weight identificat George SP., Wang Y., Mathew S., Srinivasan K., Khurana S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cross-linking agents Dithiobis (succinimidylpropionate) (DSP); Disuccinimidyl suberate (DSS); Dimethyl 3,3′-dithiobispropionimidate (DTBP); Ethylene glycol bis (succinimidylsyccinate) (EGS); 1,5-Difluoro-2,4-dinitrobenzene (DFDNB); and 3,3′ Dithiobis (sulfosuccinimidyl propionate) (DTSSP). skhurana@utmem.edu J. Biol. Chem. (0021-9258) 2007 mimix curation george-2007-2 Dimerization and actin-bundling properties of villin and its role in the assembly of epithelial cell brush borders. in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining George SP., Wang Y., Mathew S., Srinivasan K., Khurana S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cross-linking agents Dithiobis (succinimidylpropionate) (DSP); Disuccinimidyl suberate (DSS); Dimethyl 3,3′-dithiobispropionimidate (DTBP); Ethylene glycol bis (succinimidylsyccinate) (EGS); 1,5-Difluoro-2,4-dinitrobenzene (DFDNB); and 3,3′ Dithiobis (sulfosuccinimidyl propionate) (DTSSP). skhurana@utmem.edu J. Biol. Chem. (0021-9258) 2007 mimix curation george-2007-3 Dimerization and actin-bundling properties of villin and its role in the assembly of epithelial cell brush borders. in vitro In vitro density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot George SP., Wang Y., Mathew S., Srinivasan K., Khurana S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cross-linking agents Dithiobis (succinimidylpropionate) (DSP); Disuccinimidyl suberate (DSS); Dimethyl 3,3′-dithiobispropionimidate (DTBP); Ethylene glycol bis (succinimidylsyccinate) (EGS); 1,5-Difluoro-2,4-dinitrobenzene (DFDNB); and 3,3′ Dithiobis (sulfosuccinimidyl propionate) (DTSSP). skhurana@utmem.edu J. Biol. Chem. (0021-9258) 2007 mimix curation george-2007-5 Dimerization and actin-bundling properties of villin and its role in the assembly of epithelial cell brush borders. human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot George SP., Wang Y., Mathew S., Srinivasan K., Khurana S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cross-linking agents Dithiobis (succinimidylpropionate) (DSP); Disuccinimidyl suberate (DSS); Dimethyl 3,3′-dithiobispropionimidate (DTBP); Ethylene glycol bis (succinimidylsyccinate) (EGS); 1,5-Difluoro-2,4-dinitrobenzene (DFDNB); and 3,3′ Dithiobis (sulfosuccinimidyl propionate) (DTSSP). skhurana@utmem.edu J. Biol. Chem. (0021-9258) 2007 mimix curation george-2007-6 Dimerization and actin-bundling properties of villin and its role in the assembly of epithelial cell brush borders. canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line crosslink cross-linking study crosslink antibody detection identification by antibody antibody detection George SP., Wang Y., Mathew S., Srinivasan K., Khurana S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cross-linking agents Dithiobis (succinimidylpropionate) (DSP); Disuccinimidyl suberate (DSS); Dimethyl 3,3′-dithiobispropionimidate (DTBP); Ethylene glycol bis (succinimidylsyccinate) (EGS); 1,5-Difluoro-2,4-dinitrobenzene (DFDNB); and 3,3′ Dithiobis (sulfosuccinimidyl propionate) (DTSSP). skhurana@utmem.edu J. Biol. Chem. (0021-9258) 2007 mimix curation george-2007-7 Dimerization and actin-bundling properties of villin and its role in the assembly of epithelial cell brush borders. canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined George SP., Wang Y., Mathew S., Srinivasan K., Khurana S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cross-linking agents Dithiobis (succinimidylpropionate) (DSP); Disuccinimidyl suberate (DSS); Dimethyl 3,3′-dithiobispropionimidate (DTBP); Ethylene glycol bis (succinimidylsyccinate) (EGS); 1,5-Difluoro-2,4-dinitrobenzene (DFDNB); and 3,3′ Dithiobis (sulfosuccinimidyl propionate) (DTSSP). skhurana@utmem.edu J. Biol. Chem. (0021-9258) 2007 mimix curation vili_human Villin-1 VIL1 VIL protein protein human Homo sapiens Human MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWTASNRTSGLGKTHTVGSVAKVEQVKFDATSMHVKPQVAAQQKMVDDGSGEVQVWRIENLELVPVDSKWLGHFYGGDCYLLLYTYLIGEKQHYLLYVWQGSQASQDEITASAYQAVILDQKYNGEPVQIRVPMGKEPPHLMSIFKGRMVVYQGGTSRTNNLETGPSTRLFQVQGTGANNTKAFEVPARANFLNSNDVFVLKTQSCCYLWCGKGCSGDEREMAKMVADTISRTEKQVVVEGQEPANFWMALGGKAPYANTKRLQEENLVITPRLFECSNKTGRFLATEIPDFNQDDLEEDDVFLLDVWDQVFFWIGKHANEEEKKAAATTAQEYLKTHPSGRDPETPIIVVKQGHEPPTFTGWFLAWDPFKWSNTKSYEDLKAELGNSRDWSQITAEVTSPKVDVFNANSNLSSGPLPIFPLEQLVNKPVEELPEGVDPSRKEEHLSIEDFTQAFGMTPAAFSALPRWKQQNLKKEKGLF vil1-12 1023235 n/a 1023242 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 direct interaction direct interaction false false false 2D and E Monermers, dimers, trimers and tetramers habe been detected. vil1-12 1023235 n/a 1023242 unspecified role unspecified role neutral component neutral component Stoichiometry: 3.0 direct interaction direct interaction false false false 2D and E Monermers, dimers, trimers and tetramers habe been detected. vil1-12 1023235 n/a 1023242 unspecified role unspecified role neutral component neutral component Stoichiometry: 4.0 direct interaction direct interaction false false false 2D and E Monermers, dimers, trimers and tetramers habe been detected. vil1-vil1-7 1023236 n/a 1023242 unspecified role unspecified role neutral component neutral component n/a 1023242 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false 2A, 4D and E vil1-vil1-8 1023237 n/a 1023242 unspecified role unspecified role prey prey n/a 1023242 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 2B vil1-vil1-9 1023238 n/a 1023242 unspecified role unspecified role neutral component neutral component n/a 1023242 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false Monomers, dimers, trimers and possibly tetramers have been detected. 2C vil1-vil1-11 1023239 n/a 1023242 unspecified role unspecified role neutral component neutral component n/a 1023242 unspecified role unspecified role neutral component neutral component physical association physical association false false false 3B vil1-vil1-10 1023240 n/a 1023242 unspecified role unspecified role neutral component neutral component n/a 1023242 unspecified role unspecified role neutral component neutral component physical association physical association false false false 3A vil1-vil1-12 1023241 n/a 1023242 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n/a 1023242 unspecified role unspecified role fluorescence donor fluorescence donor proximity proximity close-contact colocalization neighbourhood interaction false false false 4C