IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ bottoni-2007-1 Proteasomes and RARS modulate AIMP1/EMAP II secretion in human cancer cell lines. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bottoni A., Vignali C., Piccin D., Tagliati F., Luchin A., Zatelli MC., Uberti EC. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of RARS arginyl t-RNA synthetase taken from PMID:15648093 ti8@unife.it J. Cell. Physiol. (0021-9541) 2007 Only protein-protein interactions imex curation bottoni-2007-2 Proteasomes and RARS modulate AIMP1/EMAP II secretion in human cancer cell lines. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bottoni A., Vignali C., Piccin D., Tagliati F., Luchin A., Zatelli MC., Uberti EC. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of RARS arginyl t-RNA synthetase taken from PMID:15648093 ti8@unife.it J. Cell. Physiol. (0021-9541) 2007 Only protein-protein interactions imex curation syrc_human Arginine--tRNA ligase, cytoplasmic RARS1 Arginyl-tRNA synthetase RARS protein protein human Homo sapiens Human MDVLVSECSARLLQQEEEIKSLTAEIDRLKNCGCLGASPNLEQLQEENLKLKYRLNILRKSLQAERNKPTKNMINIISRLQEVFGHAIKAAYPDLENPPLLVTPSQQAKFGDYQCNSAMGISQMLKTKEQKVNPREIAENITKHLPDNECIEKVEIAGPGFINVHLRKDFVSEQLTSLLVNGVQLPALGENKKVIVDFSSPNIAKEMHVGHLRSTIIGESISRLFEFAGYDVLRLNHVGDWGTQFGMLIAHLQDKFPDYLTVSPPIGDLQVFYKESKKRFDTEEEFKKRAYQCVVLLQGKNPDITKAWKLICDVSRQELNKIYDALDVSLIERGESFYQDRMNDIVKEFEDRGFVQVDDGRKIVFVPGCSIPLTIVKSDGGYTYDTSDLAAIKQRLFEEKADMIIYVVDNGQSVHFQTIFAAAQMIGWYDPKVTRVFHAGFGVVLGEDKKKFKTRSGETVRLMDLLGEGLKRSMDKLKEKERDKVLTAEELNAAQTSVAYGCIKYADLSHNRLNDYIFSFDKMLDDRGNTAAYLLYAFTRIRSIARLANIDEEMLQKAARETKILLDHEKEWKLGRCILRFPEILQKILDDLFLHTLCDYIYELATAFTEFYDSCYCVEKDRQTGKILKVNMWRMLLCEAVAAVMAKGFDILGIKPVQRM syk_human Lysine--tRNA ligase KARS1 KARS KIAA0070 Lysyl-tRNA synthetase protein protein human Homo sapiens Human MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV aimp1_human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 AIMP1 EMAP2 SCYE1 Multisynthase complex auxiliary component p43 protein protein human Homo sapiens Human MANNDAVLKRLEQKGAEADQIIEYLKQQVSLLKEKAILQATLREEKKLRVENAKLKKEIEELKQELIQAEIQNGVKQIPFPSGTPLHANSMVSENVIQSTAVTTVSSGTKEQIKGGTGDEKKAKEKIEKKGEKKEKKQQSIAGSADSKPIDVSRLDLRIGCIITARKHPDADSLYVEEVDVGEIAPRTVVSGLVNHVPLEQMQNRMVILLCNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK rars-aimp1 918871 n/a 918873 unspecified role unspecified role prey prey n/a 918874 unspecified role unspecified role prey prey n/a 918875 unspecified role unspecified role bait bait association association false false false Fig 2. The amount of RARS associated with AIMP1 is significantly higher in HeLa and MCF7 cells over-expressing RARS as compared to the amount of RARS associated with AIMP1 in control cells. To demonstrate that the immunoprecipitated AIMP1 was part of the ARS complex, we performed a Western blot for KARS. rars-aimp1-1 918872 n/a 918875 unspecified role unspecified role bait bait n/a 918874 unspecified role unspecified role prey prey n/a 918873 unspecified role unspecified role prey prey association association false false false Fig 2. The amount of RARS associated with AIMP1 is significantly higher in HeLa and MCF7 cells over-expressing RARS as compared to the amount of RARS associated with AIMP1 in control cells. To demonstrate that the immunoprecipitated AIMP1 was part of the ARS complex, we performed a Western blot for KARS.