IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yasui-2007-2 The TSC1 gene product hamartin interacts with NADE. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yasui S., Tsuzaki K., Ninomiya H., Floricel F., Asano Y., Maki H., Takamura A., Nanba E., Higaki K., Ohno K. Apoptosis - Interactions involving proteins with a function related to apoptosis A unique UniProtKB entry could not be associated with kinesin 2 since the exact gene product of the interactor cannot be ascertained. kh4060@grape.med.tottori-u.ac.jp A human fetal brain cDNA library was used. Mol. Cell. Neurosci. (1044-7431) 2007 Only protein-protein interactions imex curation yasui-2007-1 The TSC1 gene product hamartin interacts with NADE. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yasui S., Tsuzaki K., Ninomiya H., Floricel F., Asano Y., Maki H., Takamura A., Nanba E., Higaki K., Ohno K. Apoptosis - Interactions involving proteins with a function related to apoptosis A unique UniProtKB entry could not be associated with kinesin 2 since the exact gene product of the interactor cannot be ascertained. kh4060@grape.med.tottori-u.ac.jp A human fetal brain cDNA library was used. Mol. Cell. Neurosci. (1044-7431) 2007 Only protein-protein interactions imex curation yasui-2007-3 The TSC1 gene product hamartin interacts with NADE. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yasui S., Tsuzaki K., Ninomiya H., Floricel F., Asano Y., Maki H., Takamura A., Nanba E., Higaki K., Ohno K. Apoptosis - Interactions involving proteins with a function related to apoptosis A unique UniProtKB entry could not be associated with kinesin 2 since the exact gene product of the interactor cannot be ascertained. kh4060@grape.med.tottori-u.ac.jp A human fetal brain cDNA library was used. Mol. Cell. Neurosci. (1044-7431) 2007 Only protein-protein interactions imex curation yasui-2007-4 The TSC1 gene product hamartin interacts with NADE. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Yasui S., Tsuzaki K., Ninomiya H., Floricel F., Asano Y., Maki H., Takamura A., Nanba E., Higaki K., Ohno K. Apoptosis - Interactions involving proteins with a function related to apoptosis A unique UniProtKB entry could not be associated with kinesin 2 since the exact gene product of the interactor cannot be ascertained. kh4060@grape.med.tottori-u.ac.jp A human fetal brain cDNA library was used. Mol. Cell. Neurosci. (1044-7431) 2007 Only protein-protein interactions imex curation yasui-2007-5 The TSC1 gene product hamartin interacts with NADE. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy tag visualisation tag visualisation Yasui S., Tsuzaki K., Ninomiya H., Floricel F., Asano Y., Maki H., Takamura A., Nanba E., Higaki K., Ohno K. Apoptosis - Interactions involving proteins with a function related to apoptosis A unique UniProtKB entry could not be associated with kinesin 2 since the exact gene product of the interactor cannot be ascertained. kh4060@grape.med.tottori-u.ac.jp A human fetal brain cDNA library was used. Mol. Cell. Neurosci. (1044-7431) 2007 Only protein-protein interactions imex curation yasui-2007-6 The TSC1 gene product hamartin interacts with NADE. rat-pc12h Rattus norvegicus PC12h adrenal phaeochromocytoma cell line pc12h Rat pheochromocytoma cell line PC12h, The parental cell line is PC12. In PC12h cells a natural iridoid compound, genipin, induces neurite outgrowth through the nitric oxide (NO)-cGMP-protein kinase G signaling pathway. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yasui S., Tsuzaki K., Ninomiya H., Floricel F., Asano Y., Maki H., Takamura A., Nanba E., Higaki K., Ohno K. Apoptosis - Interactions involving proteins with a function related to apoptosis A unique UniProtKB entry could not be associated with kinesin 2 since the exact gene product of the interactor cannot be ascertained. kh4060@grape.med.tottori-u.ac.jp A human fetal brain cDNA library was used. Mol. Cell. Neurosci. (1044-7431) 2007 Only protein-protein interactions imex curation tsc1_human Hamartin TSC1 KIAA0243 Tuberous sclerosis 1 protein TSC protein protein human Homo sapiens Human MAQQANVGELLAMLDSPMLGVRDDVTAVFKENLNSDRGPMLVNTLVDYYLETSSQPALHILTTLQEPHDKHLLDRINEYVGKAATRLSILSLLGHVIRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVAEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTTSPYADTQNSYGCATSTPYSTSRLMLLNMPGQLPQTLSSPSTRLITEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLPQATVTPPRKEERMDSARPCLHRQHHLLNDRGSEEPPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISRELSEITTAEAEPVVPRGGFDSPFYRDSLPGSQRKTHSAASSSQGASVNPEPLHSSLDKLGPDTPKQAFTPIDLPCGSADESPAGDRECQTSLETSIFTPSPCKIPPPTRVGFGSGQPPPYDHLFEVALPKTAHHFVIRKTEELLKKAKGNTEEDGVPSTSPMEVLDRLIQQGADAHSKELNKLPLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYNQLQEQRDTMVTKLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQTQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLDCCNDGCSDSMVGHNEEASGHNGETKTPRPSSARGSSGSRGGGGSSSSSSELSTPEKPPHQRAGPFSSRWETTMGEASASIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDGMTSSLSESLKTELGKDLGVEAKIPLNLDGPHPSPPTPDSVGQLHIMDYNETHHEHS q6pf11_human CSH1 protein protein human Homo sapiens Human MAPGSRTSLLLAFALLCLPWLQEAGAVQTVPLSRLFDHAMLQAHRAHQLAIDTYQEFEETYIPKDQKYSFLHDSQTSFCFSDSIPTPSNMEETQQKSNLELLRISLLLIESWLEPVRFLRSMFANNLVYDTSDSDDYHLLKDLEEGIQTLMGRLEDGSRRTGQILKQTYSKFDTNSHNHDALLKNYGLLYCFRKDMDKVETFLRTVQCRSVEGSCGF bex3_human Protein BEX3 BEX3 Brain-expressed X-linked protein 3 DXS6984E NADE NGFRAP1 Nerve growth factor receptor-associated protein 1 Ovarian granulosa cell 13.0 kDa protein HGR74 p75NTR-associated cell death executor protein protein human Homo sapiens Human MANIHQENEEMEQPMQNGEEDRPLGGGEGHQPAGNRRGQARRLAPNFRWAIPNRQINDGMGGDGDDMEIFMEEMREIRRKLRELQLRNCLRILMGELSNHHDHHDEFCLMP hba_human Hemoglobin subunit alpha Hemoglobin alpha chain Alpha-globin HBA1 HBA2 protein protein human Homo sapiens Human MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR rfip5_human Rab11 family-interacting protein 5 RAB11FIP5 GAF1 KIAA0857 RIP11 Rab11-interacting protein Rip11 Gamma-SNAP-associated factor 1 Phosphoprotein pp75 protein protein human Homo sapiens Human MALVRGAEPAAGPSRWLPTHVQVTVLRARGLRGKSSGAGSTSDAYTVIQVGREKYSTSVVEKTHGCPEWREECSFELPPGALDGLLRAQEADAGPAPWAASSAAACELVLTTMHRSLIGVDKFLGQATVALDEVFGAGRAQHTQWYKLHSKPGKKEKERGEIEVTIQFTRNNLSASMFDLSMKDKPRSPFSKIRDKMKGKKKYDLESASAILPSSAIEDPDLGSLGKMGKAKGFFLRNKLRKSSLTQSNTSLGSDSTLSSASGSLAYQGPGAELLTRSPSRSSWLSTEGGRDSAQSPKLFTHKRTYSDEANQMRVAPPRALLDLQGHLDAASRSSLCVNGSHIYNEEPQGPVRHRSSISGSLPSSGSLQAVSSRFSEEGPRSTDDTWPRGSRSNSSSEAVLGQEELSAQAKVLAPGASHPGEEEGARLPEGKPVQVATPIVASSEAVAEKEGARKEERKPRMGLFHHHHQGLSRSELGRRSSLGEKGGPILGASPHHSSSGEEKAKSSWFGLREAKDPTQKPSPHPVKPLSAAPVEGSPDRKQSRSSLSIALSSGLEKLKTVTSGSIQPVTQAPQAGQMVDTKRLKDSAVLDQSAKYYHLTHDELISLLLQRERELSQRDEHVQELESYIDRLLVRIMETSPTLLQIPPGPPK cd63_human CD63 antigen Melanoma-associated antigen ME491 Ocular melanoma-associated antigen OMA81H Granulophysin Tetraspanin-30 Lysosomal-associated membrane protein 3 CD63 MLA1 TSPAN30 Lysosome integral membrane protein 1 protein protein human Homo sapiens Human MAVEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTIIQGATPGSLLPVVIIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACCLVKSIRSGYEVM nduba_human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 NDUFB10 NADH-ubiquinone oxidoreductase PDSW subunit Complex I-PDSW protein protein human Homo sapiens Human MPDSWDKDVYPEPPRRTPVQPNPIVYMMKAFDLIVDRPVTLVREFIERQHAKNRYYYYHRQYRRVPDITECKEEDIMCMYEAEMQWKRDYKVDQEIINIMQDRLKACQQREGQNYQQNCIKEVEQFTQVAKAYQDRYQDLGAYSSARKCLAKQRQRMLQERKAAKEAAAATS id3_human DNA-binding protein inhibitor ID-3 ID3 1R21 BHLHB25 Inhibitor of DNA binding 3 ID-like protein inhibitor HLH 1R21 Helix-loop-helix protein HEIR-1 HEIR1 Class B basic helix-loop-helix protein 25 Inhibitor of differentiation 3 protein protein human Homo sapiens Human MKALSPVRGCYEAVCCLSERSLAIARGRGKGPAAEEPLSLLDDMNHCYSRLRELVPGVPRGTQLSQVEILQRVIDYILDLQVVLAEPAPGPPDGPHLPIQTAELTPELVISNDKRSFCH rasa1_human Ras GTPase-activating protein 1 Ras p21 protein activator p120GAP RASA1 RASA GAP protein protein human Homo sapiens Human MMAAEAGSEEGGPVTAGAGGGGAAAGSSAYPAVCRVKIPAALPVAAAPYPGLVETGVAGTLGGGAALGSEFLGAGSVAGALGGAGLTGGGTAAGVAGAAAGVAGAAVAGPSGDMALTKLPTSLLAETLGPGGGFPPLPPPPYLPPLGAGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQMNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQEQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCNLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLRIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLTHLLNILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNTTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLILVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAITELLQQKQNQYTKTNDVR actb_human Actin, cytoplasmic 1 ACTB Beta-actin protein protein human Homo sapiens Human MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF acta_human Actin, aortic smooth muscle Alpha-actin-2 Cell growth-inhibiting gene 46 protein ACTA2 ACTSA ACTVS GIG46 protein protein human Homo sapiens Human MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF sfpq_human Splicing factor, proline- and glutamine-rich SFPQ PSF Polypyrimidine tract-binding protein-associated-splicing factor DNA-binding p52/p100 complex, 100 kDa subunit 100 kDa DNA-pairing protein protein protein human Homo sapiens Human MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRMGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF eif3f_human Eukaryotic translation initiation factor 3 subunit F Eukaryotic translation initiation factor 3 subunit 5 eIF-3-epsilon eIF3 p47 EIF3F EIF3S5 Deubiquitinating enzyme eIF3f protein protein human Homo sapiens Human MATPAVPVSAPPATPTPVPAAAPASVPAPTPAPAAAPVPAAAPASSSDPAAAAAATAAPGQTPASAQAPAQTPAPALPGPALPGPFPGGRVVRLHPVILASIVDSYERRNEGAARVIGTLLGTVDKHSVEVTNCFSVPHNESEDEVAVDMEFAKNMYELHKKVSPNELILGWYATGHDITEHSVLIHEYYSREAPNPIHLTVDTSLQNGRMSIKAYVSTLMGVPGRTMGVMFTPLTVKYAYYDTERIGVDLIMKTCFSPNRVIGLSSDLQQVGGASARIQDALSTVLQYAEDVLSGKVSADNTVGRFLMSLVNQVPKIVPDDFETMLNSNINDLLMVTYLANLTQSQIALNEKLVNL rasf7_human Ras association domain-containing protein 7 RASSF7 C11orf13 HRC1 HRAS1-related cluster protein 1 protein protein human Homo sapiens Human MLLGLAAMELKVWVDGIQRVVCGVSEQTTCQEVVIALAQAIGQTGRFVLVQRLREKERQLLPQECPVGAQATCGQFASDVQFVLRRTGPSLAGRPSSDSCPPPERCLIRASLPVKPRAALGCEPRKTLTPEPAPSLSRPGPAAPVTPTPGCCTDLRGLELRVQRNAEELGHEAFWEQELRREQAREREGQARLQALSAATAEHAARLQALDAQARALEAELQLAAEAPGPPSPMASATERLHQDLAVQERQSAEVQGSLALVSRALEAAERALQAQAQELEELNRELRQCNLQQFIQQTGAALPPPPRPDRGPPGTQGPLPPAREESLLGAPSESHAGAQPRPRGGPHDAELLEVAAAPAPEWCPLAAQPQAL frih_human Ferritin heavy chain Cell proliferation-inducing gene 15 protein FTH1 FTH FTHL6 PIG15 OK/SW-cl.84 protein protein human Homo sapiens Human MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES mat1_human CDK-activating kinase assembly factor MAT1 RING finger protein MAT1 Menage a trois CDK7/cyclin-H assembly factor p36 p35 Cyclin-G1-interacting protein RING finger protein 66 MNAT1 CAP35 MAT1 RNF66 protein protein human Homo sapiens Human MDDQGCPRCKTTKYRNPSLKLMVNVCGHTLCESCVDLLFVRGAGNCPECGTPLRKSNFRVQLFEDPTVDKEVEIRKKVLKIYNKREEDFPSLREYNDFLEEVEEIVFNLTNNVDLDNTKKKMEIYQKENKDVIQKNKLKLTREQEELEEALEVERQENEQRRLFIQKEEQLQQILKRKNKQAFLDELESSDLPVALLLAQHKDRSTQLEMQLEKPKPVKPVTFSTGIKMGQHISLAPIHKLEEALYEYQPLQIETYGPHVPELEMLGRLGYLNHVRAASPQDLAGGYTSSLACHRALQDAFSGLFWQPS sdcg8_human Serologically defined colon cancer antigen 8 Antigen NY-CO-8 Centrosomal colon cancer autoantigen protein SDCCAG8 CCCAP NPHP10 HSPC085 protein protein human Homo sapiens Human MAKSPENSTLEEILGQYQRSLREHASRSIHQLTCALKEGDVTIGEDAPNLSFSTSVGNEDARTAWPELQQSHAVNQLKDLLRQQADKESEVSPSRRRKMSPLRSLEHEETNMPTMHDLVHTINDQSQYIHHLEAEVKFCKEELSGMKNKIQVVVLENEGLQQQLKSQRQEETLREQTLLDASGNMHNSWITTGEDSGVGETSKRPFSHDNADFGKAASAGEQLELEKLKLTYEEKCEIEESQLKFLRNDLAEYQRTCEDLKEQLKHKEFLLAANTCNRVGGLCLKCAQHEAVLSQTHTNVHMQTIERLVKERDDLMSALVSVRSSLADTQQREASAYEQVKQVLQISEEANFEKTKALIQCDQLRKELERQAERLEKELASQQEKRAIEKDMMKKEITKEREYMGSKMLILSQNIAQLEAQVEKVTKEKISAINQLEEIQSQLASREMDVTKVCGEMRYQLNKTNMEKDEAEKEHREFRAKTNRDLEIKDQEIEKLRIELDESKQHLEQEQQKAALAREECLRLTELLGESEHQLHLTRQEKDSIQQSFSKEAKAQALQAQQREQELTQKIQQMEAQHDKTENEQYLLLTSQNTFLTKLKEECCTLAKKLEQISQKTRSEIAQLSQEKRYTYDKLGKLQRRNEELEEQCVQHGRVHETMKQRLRQLDKHSQATAQQLVQLLSKQNQLLLERQSLSEEVDRLRTQLPSMPQSDC tnr16_human Tumor necrosis factor receptor superfamily member 16 NGFR TNFRSF16 Low-affinity nerve growth factor receptor Gp80-LNGFR p75 ICD Low affinity neurotrophin receptor p75NTR protein protein human Homo sapiens Human MGAGATGRAMDGPRLLLLLLLGVSLGGAKEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDAVCRCAYGYYQDETTGRCEACRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECTRWADAECEEIPGRWITRSTPPEGSDSTAPSTQEPEAPPEQDLIASTVAGVVTTVMGSSQPVVTRGTTDNLIPVYCSILAAVVVGLVAYIAFKRWNSCKQNKQGANSRPVNQTPPPEGEKLHSDSGISVDSQSLHDQQPHTQTASGQALKGDGGLYSSLPPAKREEVEKLLNGSAGDTWRHLAGELGYQPEHIDSFTHEACPVRALLASWATQDSATLDALLAALRRIQRADLVESLCSESTATSPV csh1-tsc1 Hamartin interacts with Chorionic somatomammotropin hormine 1 in a yeast two-hybrid assay 662414 n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 662421 unspecified role unspecified role bait bait physical association physical association false false false Table S2 ngfrap1-tsc1 Hamartin interacts with NADE in a yeast two-hybrid assay 662414 n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 662422 unspecified role unspecified role bait bait physical association physical association false false false Table S2 hba2-tsc1 Hamartin interacts with HBA2 in a yeast two-hybrid assay 662414 n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 662423 unspecified role unspecified role bait bait physical association physical association false false false Table S2 rab11fip5-tsc1 Hamartin interacts with phosphoprotein pp75 in a yeast two-hybrid assay 662414 n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 662424 unspecified role unspecified role bait bait physical association physical association false false false Table S2 cd63-tsc1 Hamartin interacts with CD63 antigen in a yeast two-hybrid assay 662414 n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 662425 unspecified role unspecified role bait bait physical association physical association false false false Table S2 ndufb10-tsc1 Hamartin interacts with NDUFB10 in a yeast two-hybrid assay 662414 N/A 662426 unspecified role unspecified role bait bait n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S2 id3-tsc1 Hamartin interacts with ID3 in a yeast two-hybrid assay 662414 N/A 662427 unspecified role unspecified role bait bait n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S2 rasa1-tsc1 Hamartin interacts with RASA1 in a yeast two-hybrid assay 662414 N/A 662428 unspecified role unspecified role bait bait n/a 662420 unspecified role unspecified role prey prey regiojn sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S2 actb-tsc1 Hamartin interacts with actin, beta in a yeast two-hybrid assay 662414 N/A 662429 unspecified role unspecified role bait bait n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S2 acta2-tsc1 Hamartin interacts with actin, alpha 2, smooth muscle, aorta in a yeast two-hybrid assay 662414 N/A 662430 unspecified role unspecified role bait bait n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S2 sfpq-tsc1 Hamartin interacts with SFPQ in a yeast two-hybrid assay 662414 n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 662431 unspecified role unspecified role bait bait physical association physical association false false false Table S2 eif3s5-tsc1 Hamartin interacts with EIF3S5 in a yeast two-hybrid assay 662414 n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 662432 unspecified role unspecified role bait bait physical association physical association false false false Table S2 rassf7-tsc1 Hamartin interacts with RASSF7 in a yeast two-hybrid assay 662414 n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 662433 unspecified role unspecified role bait bait physical association physical association false false false Table S2 ngfrap1-tsc1-1 TSC1 (aa 674-1084) interacts with NADE in a yeast two-hybrid assay 662415 n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 662422 unspecified role unspecified role bait bait physical association physical association false false false Table S1 fth1-tsc1 Hamartin interacts with ferritin heavy polypeptide 1 in a yeast two-hybrid assay 662415 N/A 662434 unspecified role unspecified role bait bait n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S1 mnat1-tsc1 Hamartin interacts with cyclin H assembly factor in a yeast two-hybrid assay 662415 N/A 662435 unspecified role unspecified role bait bait n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S1 sdccag8-tsc1 Hamartin interacts with HSPC085 in a yeast two-hybrid assay 662415 N/A 662436 unspecified role unspecified role bait bait n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S1 tsc1-tsc1 Hamartin interacts with itself in a yeast two-hybrid assay 662415 n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 662420 unspecified role unspecified role bait bait physical association physical association false false false Table S1 ngfrap1-tsc1-2 Hamartin interacts with NADE in a yeast two-hybrid assay 662416 n/a 662420 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 662422 unspecified role unspecified role bait bait physical association physical association false false false 1 B tsc1-ngfrap1 GST-TSC1 (aa 674-1084) pulls down NADE in vitro 662417 N/A 662422 unspecified role unspecified role prey prey rat-pc12h Rattus norvegicus PC12h adrenal phaeochromocytoma cell line pc12h Rat pheochromocytoma cell line PC12h, The parental cell line is PC12. In PC12h cells a natural iridoid compound, genipin, induces neurite outgrowth through the nitric oxide (NO)-cGMP-protein kinase G signaling pathway. n/a 662420 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 1 C tsc1-ngfrap1-1 GFP-TSC1 colocalizes with myc-NADE in COS-7 cells 662418 n/a 662420 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 662422 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 2 A Q92574; Q00994 transfection Q92574 overexpressed ngfr-ngfrap1 NGFR coimmunoprecipitates Hamartin, NADE from PC12h lysates 662419 N/A 662422 unspecified role unspecified role prey prey N/A 662437 unspecified role unspecified role bait bait n/a 662420 unspecified role unspecified role prey prey association association false false false 3 A Tuberin was also coimmunoprecipitated but a unique UniProtKB identifier could not be associated with this protein.