IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chen-2007c-4 Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chen D., Zhang Z., Li M., Wang W., Li Y., Rayburn ER., Hill DL., Wang H., Zhang R. Apoptosis - Interactions involving proteins with a function related to apoptosis An email from the authors confirmed that all recombinant proteins used were of human origin. hui.wang@ccc.uab.edu,ruiwen.zhang@ccc.uab.edu A human prostate MATCHMAKER cDNA library (BD Biosciences, Clontech) was used. Oncogene (0950-9232) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer chen-2007c-1 Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Chen D., Zhang Z., Li M., Wang W., Li Y., Rayburn ER., Hill DL., Wang H., Zhang R. Apoptosis - Interactions involving proteins with a function related to apoptosis An email from the authors confirmed that all recombinant proteins used were of human origin. hui.wang@ccc.uab.edu,ruiwen.zhang@ccc.uab.edu A human prostate MATCHMAKER cDNA library (BD Biosciences, Clontech) was used. Oncogene (0950-9232) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer chen-2007c-5 Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen D., Zhang Z., Li M., Wang W., Li Y., Rayburn ER., Hill DL., Wang H., Zhang R. Apoptosis - Interactions involving proteins with a function related to apoptosis An email from the authors confirmed that all recombinant proteins used were of human origin. hui.wang@ccc.uab.edu,ruiwen.zhang@ccc.uab.edu A human prostate MATCHMAKER cDNA library (BD Biosciences, Clontech) was used. Oncogene (0950-9232) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer chen-2007c-2 Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Chen D., Zhang Z., Li M., Wang W., Li Y., Rayburn ER., Hill DL., Wang H., Zhang R. Apoptosis - Interactions involving proteins with a function related to apoptosis An email from the authors confirmed that all recombinant proteins used were of human origin. hui.wang@ccc.uab.edu,ruiwen.zhang@ccc.uab.edu A human prostate MATCHMAKER cDNA library (BD Biosciences, Clontech) was used. Oncogene (0950-9232) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer chen-2007c-3 Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Chen D., Zhang Z., Li M., Wang W., Li Y., Rayburn ER., Hill DL., Wang H., Zhang R. Apoptosis - Interactions involving proteins with a function related to apoptosis An email from the authors confirmed that all recombinant proteins used were of human origin. hui.wang@ccc.uab.edu,ruiwen.zhang@ccc.uab.edu A human prostate MATCHMAKER cDNA library (BD Biosciences, Clontech) was used. Oncogene (0950-9232) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer rs7_human 40S ribosomal protein S7 RPS7 Small ribosomal subunit protein eS7 protein protein human Homo sapiens Human MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLVRELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDKAQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD rl11_human 60S ribosomal protein L11 RPL11 CLL-associated antigen KW-12 Large ribosomal subunit protein uL5 protein protein human Homo sapiens Human MAQDQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKEEAMRWFQQKYDGIILPGK rl23_human 60S ribosomal protein L23 RPL23 60S ribosomal protein L17 Large ribosomal subunit protein uL14 protein protein human Homo sapiens Human MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA mdm2-rps7-6 T7-MDM2 coimmunoprecipitates myc-S7 in COS-7 lysates 659480 N/A 659485 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 659486 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 C2 Q00987; P62081 transfection rps7-mdm2-1 T7-MDM2 coimmunoprecipitates with myc-S7 in COS-7 lysates 659480 N/A 659485 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 659486 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 C1 Q00987; P62081 transfection mdm2-rps7-7 myc-MDM2 coimmunoprecipitates GFP-S7 in COS-7 lysates 659480 N/A 659485 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 659486 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 G1 Q00987; P62081 transfection rps7-mdm2-3 myc-MDM2 coimmunoprecipitates with GFP-S7 in COS-7 lysates 659480 N/A 659485 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 659486 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 G2 Q00987; P62081 transfection rps7-mdm2-4 T7-MDM2 coimmunoprecipitates with GFP-S7 in COS-7 lysates 659480 N/A 659485 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 659486 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3 C1 Q00987; P62081 transfection Q00987; P62081 overexpressed rps7-mdm2-5 T7-MDM2 and HA-p53 coimmunoprecipitate with GFP-S7 in COS-7 lysates 659480 N/A 659485 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 659487 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 659486 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 3 C1 Q00987; P62081; P04637 transfection Q00987; P62081; P04637 overexpressed mdm2-rps7-2 MDM2 interacts with ribosomal protein S7 in a yeast two-hybrid assay 659481 N/A 659485 unspecified role unspecified role prey prey N/A 659486 unspecified role unspecified role bait bait physical association physical association false false false mdm2-rpl11-1 MDM2 interacts with RPL11 in a yeast two-hybrid assay 659481 N/A 659486 unspecified role unspecified role bait bait N/A 659488 unspecified role unspecified role prey prey physical association physical association false false false mdm2-rpl23 MDM2 interacts with RPL23 in a yeast two-hybrid assay 659481 N/A 659486 unspecified role unspecified role bait bait N/A 659489 unspecified role unspecified role prey prey physical association physical association false false false mdm2-rps7-1 MDM2 coimmunoprecipitates S7 in U2OS lysates 659482 N/A 659485 unspecified role unspecified role prey prey N/A 659486 unspecified role unspecified role bait bait physical association physical association false false false 1 C2 rps7-mdm2-2 MDM2 coimmunoprecipitates with S7 in U2OS lysates 659482 N/A 659485 unspecified role unspecified role bait bait N/A 659486 unspecified role unspecified role prey prey physical association physical association false false false 1 C1 mdm2-rps7 MDM2 coimmunoprecipitates S7 and p53 in U2OS lysates 659482 N/A 659485 unspecified role unspecified role prey prey N/A 659487 unspecified role unspecified role prey prey N/A 659486 unspecified role unspecified role bait bait association association false false false 3 D Actinomycin D (5 nM for 24 h) enhanced this interaction. This treatment causes ribosomal stress. mdm2-rps7-3 GST-MDM2 pulls down ribosomal protein S7 in vitro 659483 N/A 659485 unspecified role unspecified role prey prey N/A 659486 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 1 B1 rps7-mdm2 GST-S7 pulls down MDM2 in vitro 659483 N/A 659485 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 659486 unspecified role unspecified role prey prey direct interaction direct interaction false false false 1 B2 mdm2-rps7-4 MDM2 interacts with ribosomal protein S7 in a yeast two-hybrid assay 659484 N/A 659485 unspecified role unspecified role prey prey N/A 659486 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 A, 1 E1, E2, E3 mdm2-rps7-5 MDM2 interacts with ribosomal protein S7 in a yeast two-hybrid assay 659484 N/A 659485 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 659486 unspecified role unspecified role bait bait physical association physical association false false false 1 A, 1 F1, F2, F3