IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ grimmler-2007-2 Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis Further detail on Lyn kinase taken from manufacturer's website. Identity of Cyclin A taken from reference Lacy et al. 2004 (PMID:15024385). ludger.hengst@i-med.ac.at Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation grimmler-2007-4 Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis Identity of Cyclin A taken from reference Lacy et al. 2004 (PMID:15024385). ludger.hengst@i-med.ac.at Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation grimmler-2007-1 Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation grimmler-2007-5 Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation grimmler-2007-3 Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis Identity of Cyclin A taken from reference Lacy et al. 2004 (PMID:15024385). ludger.hengst@i-med.ac.at Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation grimmler-2007-6 Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation lyn_human Tyrosine-protein kinase Lyn JTK8 Lck/Yes-related novel protein tyrosine kinase V-yes-1 Yamaguchi sarcoma viral related oncogene homolog p53Lyn p56Lyn LYN protein protein human Homo sapiens Human MGCIKSKGKDSLSDDGVDLKTQPVRNTERTIYVRDPTSNKQQRPVPESQLLPGQRFQTKDPEEQGDIVVALYPYDGIHPDDLSFKKGEKMKVLEEHGEWWKAKSLLTKKEGFIPSNYVAKLNTLETEEWFFKDITRKDAERQLLAPGNSAGAFLIRESETLKGSFSLSVRDFDPVHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKHYQKQADGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKRLGAGQFGEVWMGYYNNSTKVAVKTLKPGTMSVQAFLEEANLMKTLQHDKLVRLYAVVTREEPIYIITEYMAKGSLLDFLKSDEGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCFTIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMTALSQGYRMPRVENCPDELYDIMKMCWKEKAEERPTFDYLQSVLDDFYTATEGQYQQQP cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT abl1_mouse Tyrosine-protein kinase ABL1 Abl1 Abl Abelson murine leukemia viral oncogene homolog 1 Proto-oncogene c-Abl p150 Abelson tyrosine-protein kinase 1 protein protein mouse Mus musculus Mouse MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTIYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVSAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKRGTRGGAGSMLQAPELPTKTRTCRRAAEQKDAPDTPELLHTKGLGESDALDSEPAVSPLLPRKERGPPDGSLNEDERLLPRDRKTNLFSALIKKKKKMAPTPPKRSSSFREMDGQPDRRGASEDDSRELCNGPPALTSDAAEPTKSPKASNGAGVPNGAFREPGNSGFRSPHMWKKSSTLTGSRLAAAEEESGMSSSKRFLRSCSASCMPHGARDTEWRSVTLPRDLPSAGKQFDSSTFGGHKSEKPALPRKRTSESRSEQVAKSTAMPPPRLVKKNEEAAEEGFKDTESSPGSSPPSLTPKLLRRQVTASPSSGLSHKEEATKGSASGMGTPATAEPAPPSNKVGLSKASSEEMRVRRHKHSSESPGRDKGRLAKLKPAPPPPPACTGKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTDPTKAGPPGEGLRKPVPPSVPKPQSTAKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLISTRVSLRKTRQPPERIASGTITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLESNLRELQICPATASSGPAATQDFSKLLSSVKEISDIVRR ccna2_human Cyclin-A2 Cyclin A CCNA2 CCN1 CCNA protein protein human Homo sapiens Human MLGNSAPGPATREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRGLAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKIEREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLKVLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYHGVSLLNPPETLNL cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL ccne1_human G1/S-specific cyclin-E1 CCNE1 CCNE protein protein human Homo sapiens Human MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA ccnd1_human G1/S-specific cyclin-D1 CCND1 BCL1 PRAD1 PRAD1 oncogene BCL-1 oncogene B-cell lymphoma 1 protein protein protein human Homo sapiens Human MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI cdkn1b-lyn-2 Phosphorylation of CDKN1B (p27) by lyn 593342 N/A 593348 enzyme enzyme unspecified role unspecified role n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. N/A 593349 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 1D Phosphorylation of CDKN1B (p27) on Tyr-88 (mutational analysis) cdkn1b-abl-1 Phosphorylation of CDKN1B (p27) by murine c-Abl 593342 N/A 593350 enzyme enzyme unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false ecoli Escherichia coli (strain K12) N/A 593349 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 1D Phosphorylation of CDKN1B (p27) on Tyr-88 (mutational analysis) cdkn1b-abl-2 Phosphorylation of CDKN1B (p27) by murine c-Abl 593342 N/A 593349 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) N/A 593351 unspecified role unspecified role ancillary ancillary N/A 593352 unspecified role unspecified role ancillary ancillary spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 593350 enzyme enzyme unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 1G Phosphorylation of CDKN1B (p27) Binding of CDKN1B (p27) to Cyclin A/CDK2 partially inhibited phosphorylation by cAbl cdkn1b-cdk2-2 interaction between CDKN1B, CDK2 and CCNA2 demonstrated by SPR 593343 N/A 593349 unspecified role unspecified role bait bait P46527:p.Tyr89Phe mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 593351 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 593352 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false Table 1 Tyr-88 of CDKN1B (p27) tyrosine phosphorylated, KD=3.8E-9M. Tyr-88 and Tyr 89 of CDKN1B (p27) mutated to Phe KD=5.8E-9M. Truncated CDKN1B (p27) 22-104, KD=2.9E-9M. Tyr-88 of truncated CDKN1B (p27) 22-104, KD=2.7E-9M. cdkn1b-cdk2-3 interaction between CDKN1B and CDK2 demonstrated by SPR 593343 N/A 593349 unspecified role unspecified role bait bait P46527:p.Tyr89Phe mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 593352 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction false false false Table 1 Tyr-88 of CDKN1B (p27) tyrosine phosphorylated, KD=4.5E-6M. Truncated 22-104 CDKN1B (p27)KD=1.9E-7M Tyr-88 of truncated 22-104 CDKN1B (p27)tyrosine phosphorylated, KD=9.0E-7M. cdkn1b-ccna1-1 interaction between CDKN1B and CCNA2 demonstrated by SPR 593343 N/A 593349 unspecified role unspecified role bait bait P46527:p.Tyr89Phe mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 593351 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction false false false Table 1 Tyr-88 of CDKN1B (p27) tyrosine phosphorylated, KD=4.0E-8M. Tyr-88 of truncated 22-104 CDKN1B (p27)tyrosine phosphorylated, KD=3.03E-8M. Truncated 22-104 CDKN1B (p27) KD=3.87E-8M. cdkn1b-lyn-1 Interaction between CDKN1B (p27) and lyn demonstrated by coimmunoprecipitation 593344 N/A 593348 unspecified role unspecified role prey prey P07948:p.Tyr508Pro mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 593349 unspecified role unspecified role bait bait P46527:p.Pro94_Lys96delinsAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P46527:p.[Tyr74Ala;Tyr88Ala;Tyr89Ala] mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Catalytically active Lyn (Tyr508Phe) will only bind mutated CDKN1B (p27). Inactive endogenous Lyn binds endogenous p27. 1B cdkn1b-cdk2-5 Phosphorylation of CDKN1B by CDK2 and CCNA2 593344 N/A 593349 enzyme target enzyme target substrate substrate unspecified role unspecified role P46527:p.Tyr89Phe mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 593351 unspecified role unspecified role ancillary ancillary spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 593352 enzyme enzyme unspecified role unspecified role spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation false false false Fig. 4 Prior phosphorylation of Tyr-88 of CDKN1B increases the rate of phosphorylation of the molecule by CDK2 ccne1-cdkn1b-1 Interaction between Cyclin E1 and CDKN1B (p27) by coimmunoprecipitation 593345 N/A 593349 unspecified role unspecified role prey prey try-88 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false N/A 593353 unspecified role unspecified role bait bait physical association physical association false false false 2B ccnd1-cdkn1b-2 Interaction between Cyclin D1 and CDKN1B (p27) by coimmunoprecipitation 593345 N/A 593354 unspecified role unspecified role bait bait N/A 593349 unspecified role unspecified role prey prey tyr-88 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2B cdkn1b-cdk2-1 interaction between CDKN1B, CDK2 and CCNA2 demonstrated by gel filtration 593346 N/A 593351 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 593352 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 593349 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false 2A Both phosphorylated and dephosphorylated p27 bind to Cyclin A2/CDK2 cdkn1b-cdk2-4 interaction between CDKN1B, CDK2 and CCNA2 demonstrated by NMR 593347 N/A 593351 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 593349 unspecified role unspecified role neutral component neutral component P46527:p.Tyr89Phe mutation mutation certain certain sequence position certain certain certain sequence position certain false kid region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 593352 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false Figures 3B, S8C, and S8D