DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ribeil-2007-1 Hsp70 regulates erythropoiesis by preventing caspase-3-mediated cleavage of GATA-1. human-erythroblast Human erythroblast cells-immature red blood cell with a nucleus erythroblast ERYTHROBLAST-immature red blood cell with a nucleus confocal microscopy confocal microscopy immunostaining immunostaining Ribeil J.A., Zermati Y., Vandekerckhove J., Cathelin S., Kersual J., Dussiot M., Coulon S., Moura I.C., Zeuner A., Kirkegaard-Sorensen T., Varet B., Solary E., Garrido C., Hermine O. Apoptosis - Interactions involving proteins with a function related to apoptosis The glycophorin-A+ erythroid progenitors in this experiment were coming from fresh bone marrow. hermine@necker.fr; zermati@igr.fr Nature (0028-0836) 2007 Only protein-protein interactions imex curation ribeil-2007-5 Hsp70 regulates erythropoiesis by preventing caspase-3-mediated cleavage of GATA-1. in vitro In vitro protease assay protease assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ribeil J.A., Zermati Y., Vandekerckhove J., Cathelin S., Kersual J., Dussiot M., Coulon S., Moura I.C., Zeuner A., Kirkegaard-Sorensen T., Varet B., Solary E., Garrido C., Hermine O. Apoptosis - Interactions involving proteins with a function related to apoptosis The glycophorin-A+ erythroid progenitors in this experiment were coming from fresh bone marrow. hermine@necker.fr; zermati@igr.fr Nature (0028-0836) 2007 Only protein-protein interactions imex curation ribeil-2007-2 Hsp70 regulates erythropoiesis by preventing caspase-3-mediated cleavage of GATA-1. human-bone marrow Human bone marrow bone_marrow bone marrow confocal microscopy confocal microscopy immunostaining immunostaining Ribeil J.A., Zermati Y., Vandekerckhove J., Cathelin S., Kersual J., Dussiot M., Coulon S., Moura I.C., Zeuner A., Kirkegaard-Sorensen T., Varet B., Solary E., Garrido C., Hermine O. Apoptosis - Interactions involving proteins with a function related to apoptosis The glycophorin-A+ erythroid progenitors in this experiment were coming from fresh bone marrow. hermine@necker.fr; zermati@igr.fr Nature (0028-0836) 2007 Only protein-protein interactions imex curation ribeil-2007-3 Hsp70 regulates erythropoiesis by preventing caspase-3-mediated cleavage of GATA-1. human-erythroblast Human erythroblast cells-immature red blood cell with a nucleus erythroblast ERYTHROBLAST-immature red blood cell with a nucleus anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ribeil J.A., Zermati Y., Vandekerckhove J., Cathelin S., Kersual J., Dussiot M., Coulon S., Moura I.C., Zeuner A., Kirkegaard-Sorensen T., Varet B., Solary E., Garrido C., Hermine O. Apoptosis - Interactions involving proteins with a function related to apoptosis The glycophorin-A+ erythroid progenitors in this experiment were coming from fresh bone marrow. hermine@necker.fr; zermati@igr.fr Nature (0028-0836) 2007 Only protein-protein interactions imex curation ribeil-2007-4 Hsp70 regulates erythropoiesis by preventing caspase-3-mediated cleavage of GATA-1. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ribeil J.A., Zermati Y., Vandekerckhove J., Cathelin S., Kersual J., Dussiot M., Coulon S., Moura I.C., Zeuner A., Kirkegaard-Sorensen T., Varet B., Solary E., Garrido C., Hermine O. Apoptosis - Interactions involving proteins with a function related to apoptosis The glycophorin-A+ erythroid progenitors in this experiment were coming from fresh bone marrow. hermine@necker.fr; zermati@igr.fr Nature (0028-0836) 2007 Only protein-protein interactions imex curation ribeil-2007-6 Hsp70 regulates erythropoiesis by preventing caspase-3-mediated cleavage of GATA-1. human-erythroblast Human erythroblast cells-immature red blood cell with a nucleus erythroblast ERYTHROBLAST-immature red blood cell with a nucleus confocal microscopy confocal microscopy immunostaining immunostaining Ribeil J.A., Zermati Y., Vandekerckhove J., Cathelin S., Kersual J., Dussiot M., Coulon S., Moura I.C., Zeuner A., Kirkegaard-Sorensen T., Varet B., Solary E., Garrido C., Hermine O. Apoptosis - Interactions involving proteins with a function related to apoptosis The glycophorin-A+ erythroid progenitors in this experiment were coming from fresh bone marrow. hermine@necker.fr; zermati@igr.fr Nature (0028-0836) 2007 Only protein-protein interactions imex curation gata1_human Erythroid transcription factor GATA1 ERYF1 GF1 Eryf1 GATA-binding factor 1 NF-E1 DNA-binding protein protein protein human Homo sapiens Human MEFPGLGSLGTSEPLPQFVDPALVSSTPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALPSSLPVPNSAYGGPDFSSTFFSPTGSPLNSAAYSSPKLRGTLPLPPCEARECVNCGATATPLWRRDRTGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCTNCQTTTTTLWRRNASGDPVCNACGLYYKLHQVNRPLTMRKDGIQTRNRKASGKGKKKRGSSLGGTGAAEGPAGGFMVVAGGSGSGNCGEVASGLTLGPPGTAHLYQGLGPVVLSGPVSHLMPFPGPLLGSPTGSFPTGPMPPTTSTTVVAPLSS casp3_human Caspase-3 Apopain Cysteine protease CPP32 Protein Yama SREBP cleavage activity 1 CASP3 CPP32 protein protein human Homo sapiens Human MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH hsp71_human Heat shock 70 kDa protein 1A/1B HSPA1A HSPA1 HSPA1B Heat shock 70 kDa protein 1/2 HSX70 protein protein human Homo sapiens Human MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD parp1_human Poly [ADP-ribose] polymerase 1 PARP1 ADPRT PPOL NAD(+) ADP-ribosyltransferase 1 Poly[ADP-ribose] synthase 1 ADP-ribosyltransferase diphtheria toxin-like 1 DNA ADP-ribosyltransferase PARP1 Protein poly-ADP-ribosyltransferase PARP1 protein protein human Homo sapiens Human MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW casp3-gata1 1214858 n/a 1214864 unspecified role unspecified role neutral component neutral component n/a 1214865 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1a Differentiating cells deprived of Epo (erythropoietin) show increased colocalization. hsp70-gata1-1 1214858 n/a 1214866 unspecified role unspecified role neutral component neutral component n/a 1214864 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1c, 1e and 3a Treatment of cells with Epo (erythropoietin) is required for the colocalization to be detected. Leptomycin treatment (prevents nuclear export of Hsp70) allows the interaction to happen without the presence of Epo. casp3-gata1-5 1214859 n/a 1214865 enzyme enzyme unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) n/a 1214864 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage false false false P42574 (GO:0004197) down-regulates quantity P15976 2d and 2e Recombinant Hsp70 can block the GATA-1 cleavage by CASp3 in a dose dependent manner. This blocking action depends on the Hsp70-GATA-1 interaction, as DelPDB-Hsp70 mutants are unable to inhibit the cleavage. casp3-parp1-3 1214859 n/a 1214865 enzyme enzyme unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) n/a 1214867 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage false false false P42574 (GO:0004197) down-regulates quantity P09874 2d and 2e Recombinant Hsp70 can block the GATA-1 cleavage by CASp3 in a dose dependent manner. This blocking action depends on the Hsp70-GATA-1 interaction, as DelPDB-Hsp70 mutants are unable to inhibit the cleavage. gata1-hspa1a 1214860 n/a 1214866 unspecified role unspecified role neutral component neutral component n/a 1214864 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S3 gata1-hspa1a-2 1214861 n/a 1214864 unspecified role unspecified role bait bait n/a 1214866 unspecified role unspecified role prey prey physical association physical association false false false 2a, S4 Treatment of cells with Epo (erythropoietin) is required for the interaction to be detectable under normal conditions. An increase in the input allows the interaction to be detected even in the absence of Epo due to remnant nuclear Hsp70. gata1-hspa1a-3 1214862 n/a 1214866 unspecified role unspecified role prey prey pdb domain required to bind necessary binding region required to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1214864 unspecified role unspecified role bait bait physical association physical association false false false 2c Treatment of cells with Epo (erythropoietin) is required for the interaction to happen. hsp70-gata1-2 1214863 n/a 1214866 unspecified role unspecified role neutral component neutral component n/a 1214864 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4a