UniProt UniProt http://www.uniprot.org shirane-2006-4 Protrudin induces neurite formation by directional membrane trafficking. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp Human brain cDNA Science (0036-8075) 2006 mimix curation shirane-2006-5 Protrudin induces neurite formation by directional membrane trafficking. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp Human brain cDNA Science (0036-8075) 2006 mimix curation shirane-2006-1 Protrudin induces neurite formation by directional membrane trafficking. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp Human brain cDNA Science (0036-8075) 2006 mimix curation shirane-2006-2 Protrudin induces neurite formation by directional membrane trafficking. rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp Human brain cDNA Science (0036-8075) 2006 mimix curation shirane-2006-3 Protrudin induces neurite formation by directional membrane trafficking. rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shirane M., Nakayama KI. Apoptosis - Interactions involving proteins with a function related to apoptosis nakayak1@bioreg.kyushu-u.ac.jp Human brain cDNA Science (0036-8075) 2006 mimix curation zfy27_human Protrudin Spastic paraplegia 33 protein SPG33 Zinc finger FYVE domain-containing protein 27 ZFYVE27 protein protein human Homo sapiens Human MQTSEREGSGPELSPSVMPEAPLESPPFPTKSPAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRWQMPLCSLLTCLGLNVLFLTLNEGAWYSVGALMISVPALLGYLQEVCRARLPDSELMRRKYHSVRQEDLQRGRLSRPEAVAEVKSFLIQLEAFLSRLCCTCEAAYRVLHWENPVVSSQFYGALLGTVCMLYLLPLCWVLTLLNSTLFLGNVEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK rb11a_human Ras-related protein Rab-11A YL8 RAB11A RAB11 protein protein human Homo sapiens Human MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI rfip2_human Rab11 family-interacting protein 2 NRip11 RAB11FIP2 KIAA0941 protein protein human Homo sapiens Human MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPGLLIQGSPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRIKNRGEIKVNIQFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKNDGTFSDTSSAIIPSTHMPDANSEFSSGEIQMKSKPKKPFLLGPQRLSSAHSMSDLSGSHMSSEKLKAGTIGQTHLLGHQLDSFGTVPESGSLKSPHRRTLSFDTSKMNQPDSIVDEGELCFGRQNDPFTNVTASLPQKFATLPRKKNPFEESSETWDSSMNLFSKPIEIRKENKREKREKVSLFERVTGKKDSRRSDKLNNGGSDSPCDLKSPNAFSENRQDYFDYESTNPFTAKFRASNIMPSSSFHMSPTSNEDLRKIPDSNPFDATAGYRSLTYEEVLQELVKHKELLRRKDTHIRELEDYIDNLLVRVMEETPSILRVPYEPSRKAGKFSNS fkbp8_human Peptidyl-prolyl cis-trans isomerase FKBP8 FK506-binding protein 8 38 kDa FK506-binding protein FKBPR38 FKBP8 FKBP38 Rotamase protein protein human Homo sapiens Human MASCAEPSEPSAPLPAGVPPLEDFEVLDGVEDAEGEEEEEEEEEEEDDLSELPPLEDMGQPPAEEAEQPGALAREFLAAMEPEPAPAPAPEEWLDILGNGLLRKKTLVPGPPGSSRPVKGQVVTVHLQTSLENGTRVQEEPELVFTLGDCDVIQALDLSVPLMDVGETAMVTADSKYCYGPQGRSPYIPPHAALCLEVTLKTAVDGPDLEMLTGQERVALANRKRECGNAHYQRADFVLAANSYDLAIKAITSSAKVDMTFEEEAQLLQLKVKCLNNLAASQLKLDHYRAALRSCSLVLEHQPDNIKALFRKGKVLAQQGEYSEAIPILRAALKLEPSNKTIHAELSKLVKKHAAQRSTETALYRKMLGNPSRLPAKCPGKGAWSIPWKWLFGATAVALGGVALSVVIAARN zfy27_rat Protrudin Zinc finger FYVE domain-containing protein 27 Zfyve27 protein protein rat Rattus norvegicus (Rat) Rat MQSSDRDLSGPEASPSVMPEVLSECSPAPTKSTAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRWQMPLCSLLTCLGLNILFLTLNEGAWYSVGALIISVPALLGYLQEVCRAQLPESELMRRKYHSVRQEDLQRVRLSRPEAVAEVKSFLIRLEAFLARLCYTCESAYRVLHWENPVVSSQFYGALLGMVCMLYLLPLCWVLALLNSTLFLGNGEFFRVVSEYRACLQRRMSPKQEECVCEGSALQDAGGRAVVLDSTPAPTPTEDLTPGSVEEAEEAEPDEEFKDAIEEDDEGTPCPAEDELTMQDNGFLSKNEVLRSKVSKLTERLRKRYPTNNFGNCAGCAATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK rb11a_rat Ras-related protein Rab-11A Rab11a Rab11 24KG protein protein rat Rattus norvegicus (Rat) Rat MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI zfy27-rab11a 1142490 n/a 1142495 unspecified role unspecified role bait bait n/a 1142496 unspecified role unspecified role prey prey physical association physical association false false false 3C rab11a-zfy27-1 1142490 n/a 1142495 unspecified role unspecified role prey prey n/a 1142496 unspecified role unspecified role bait bait physical association physical association false false false S4A rab11a-rab11fip2 1142490 n/a 1142496 unspecified role unspecified role bait bait n/a 1142497 unspecified role unspecified role prey prey physical association physical association false false false S4A zfy27-rab11a-1 1142491 n/a 1142495 unspecified role unspecified role bait bait spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection n/a 1142496 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false S4C rab11fip2-rab11a 1142491 n/a 1142497 unspecified role unspecified role bait bait spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection n/a 1142496 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false S4C fkbp8-zfy27 1142492 n/a 1142495 unspecified role unspecified role prey prey n/a 1142498 unspecified role unspecified role bait bait physical association physical association false false false materials and methods rab11a-zfy27 1142493 n/a 1142495 unspecified role unspecified role neutral component neutral component n/a 1142496 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false fig S3 q6p7b7-rab11a 1142494 n/a 1142499 unspecified role unspecified role bait bait n/a 1142500 unspecified role unspecified role prey prey physical association physical association false false false 3B