IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tong-2006-1 Keratin 17 modulates hair follicle cycling in a TNFalpha-dependent fashion. mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tong X., Coulombe PA. Apoptosis - Interactions involving proteins with a function related to apoptosis coulombe@jhmi.edu Genes Dev. (0890-9369) 2006 Only protein-protein interactions imex curation tong-2006-2 Keratin 17 modulates hair follicle cycling in a TNFalpha-dependent fashion. mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Tong X., Coulombe PA. Apoptosis - Interactions involving proteins with a function related to apoptosis coulombe@jhmi.edu Genes Dev. (0890-9369) 2006 Only protein-protein interactions imex curation k1c17_mouse Keratin, type I cytoskeletal 17 Krt17 Krt1-17 Keratin-17 Cytokeratin-17 protein protein mouse Mus musculus Mouse MTTTIRQFTSSSSIKGSSGLGGGSSRTSCRLSGSLGAGSCRLGSASGLGSALGSNSYSSCYSFGTGSGYGGNFGGVDGLLAGGEKATMQNLNDRLASYLDKVRALEEANTELEVKIRDWYQKQAPGPARDYSAYYHTIEDLKNKILVATVDNASILLQIDNARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLSRILSEMRDQYEKMAEKNRKDAEDWFFSKTEELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGSLAETENRYCVQLSQIQGLIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKPKEPVTTRQVRTIVEEVQDGKVISSREQVHQTTR tradd_mouse Tumor necrosis factor receptor type 1-associated DEATH domain protein Tradd TNFRSF1A-associated via death domain protein protein mouse Mus musculus Mouse MAAGQNGHEEWVGSAYLFLESAVDKVILSEAYTDPKKKVAIYKALQTALSESGDSSDVLQILKIHCSDPQLIVQLRFCGRVLCGRFLQAYREGALRTALQRCMAPALAQEALRLQLELRAGAEQLDSWLTDEERCLNYILAQKPDRLRDEELAELEDELCKLTCDCTGQGGAIQVASAGSKFPVSSPTEEKPLPAACQTFLFHGQLVVNRPLTLQDQQTFARSVGLKWRRVGRSLQRNCRALRDPALDSLAYEYERDGLYEQAFQLLRRFMQAEGRRATLQRLVEALEENELTSLAEDLLGQAEPDGGLA k1c16_mouse Keratin, type I cytoskeletal 16 Krt16 Krt1-16 Cytokeratin-16 Keratin-16 protein protein mouse Mus musculus Mouse MATCSRQFTSSSSMKGSCGIGGGSSRMSSILAGGSCRAPSTCGGMSVTSSRFSSGGVCGIGGGYGGSFSSSSFGGGLGSGFGGRFDGFGGGFGAGLGGGLGGGIGDGLLVGSEKVTMQNLNDRLATYLDKVRALEEANRDLEVKIRDWYQRQRPTEIKDYSPYFKTIEDLKSKIIIATQENAQFTLQIDNARLAADDFRTKYENELFLRQSVEGDINGLRKVLDELTLSRADLEMQIENLREELAFLKKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRDQYEQMAEKNRRDVEAWFLRKTEELNKEVASNSDLIQSNRSEVAELRRVFQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLISSVEEQLAQLRCEMEQQSQEYNILLDVKTRLEQEIATYRRLLDGENIHSSSQHSSGQSYSSREVFSSSSRQPRSILKEQGSTSFSQSQSQSSRD k1c14_mouse Keratin, type I cytoskeletal 14 Krt14 Krt1-14 Cytokeratin-14 Keratin-14 protein protein mouse Mus musculus Mouse MATCSRQFTSSSSMKGSCGIGGGSSRMSSILAGGSCRAPSTYGGMSVTSSRFSSGGACGIGGGYGGSFSSSSFGGGLGSGFGGRFDGFGGGFGGGLGGGFGGGLGGGLGGGIGDGLLVGSEKVTMQNLNDRLATYLDKVRALEEANTELEVKIRDWYQRQRPTEIKDYSPYFKTIEDLKSKILAATVDNANVLLQIDNARLAADDFRTKFETEQSLRMSVEADINGLRRVLDELTLARADLEMQIESLKEELAYLKKNHEEEMASMRGQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFSKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENNLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSSSQFSSGSQSSRDVTSTNRQIRTKVMDVHDGKVVSTHEQVLRTKN krt17-tradd CoIP showing interaction between Krt17 and endogenous TRADD 671901 N/A 671903 unspecified role unspecified role prey prey mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] N/A 671904 unspecified role unspecified role bait bait mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] physical association physical association false false false Figure 3A,A' Krt17 associates with endogenous TRADD. krt16-tradd CoIP showing interaction between Krt16 and endogenous TRADD 671901 N/A 671905 unspecified role unspecified role prey prey mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] N/A 671904 unspecified role unspecified role bait bait mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] physical association physical association false false false Figure 3A,A' Krt16 associates with endogenous TRADD. krt14-tradd CoIP showing interaction between Krt14 and endogenous TRADD 671901 N/A 671906 unspecified role unspecified role prey prey mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] N/A 671904 unspecified role unspecified role bait bait mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] physical association physical association false false false Figure 3A,A' Krt14 associates with endogenous TRADD. krt17-tradd-1 Colocalization showing interaction between Krt17 and TRADD 671902 N/A 671904 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] N/A 671903 unspecified role unspecified role prey prey mouse-epithelium Mus musculus epithelium Epithelium [epithelial cells] physical association physical association false false false Figure 3B-B", 3C-C". It was observed that both full-length TRADD (AA 1-312) and its C-terminal moiety (AA 105-312) partially colocalize with K17 in the cytoplasm. In contrast, the N-terminus of TRADD (AA 1-105) showed a diffuse distribution in the cytoplasm and nucleus.