IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ munguia-2006-1 Identification of amyloid-beta 1-42 binding protein fragments by screening of a human brain cDNA library. in vitro In vitro phage display phage display nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Munguia ME., Govezensky T., Martinez R., Manoutcharian K., Gevorkian G. Apoptosis - Interactions involving proteins with a function related to apoptosis 10exp10 phage bearing a C-terminal peptide domain of Complex I ND3 was bound to each well of a microtiter plates. Biotinylated amyloid-beta 1-42 was added to each well, an avidine-peroxidase conjugate was then used to identify prey protein. M13 phage was used as a negative control phage. gokar@servidor.unam.mx Human brain cDNA library displayed on M13 phage Neurosci. Lett. (0304-3940) 2006 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease munguia-2006-2 Identification of amyloid-beta 1-42 binding protein fragments by screening of a human brain cDNA library. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa predetermined predetermined participant predetermined Munguia ME., Govezensky T., Martinez R., Manoutcharian K., Gevorkian G. Apoptosis - Interactions involving proteins with a function related to apoptosis 10exp10 phage bearing a C-terminal peptide domain of Complex I ND3 was bound to each well of a microtiter plates. Biotinylated amyloid-beta 1-42 was added to each well, an avidine-peroxidase conjugate was then used to identify prey protein. M13 phage was used as a negative control phage. gokar@servidor.unam.mx Human brain cDNA library displayed on M13 phage Neurosci. Lett. (0304-3940) 2006 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease nu3m_human NADH-ubiquinone oxidoreductase chain 3 MT-ND3 MTND3 NADH3 ND3 NADH dehydrogenase subunit 3 protein protein human Homo sapiens Human MNFALILMINTLLALLLMIITFWLPQLNGYMEKSTPYECGFDPMSPARVPFSMKFFLVAITFLLFDLEIALLLPLPWALQTTNLPLMVMSSLLLIIILALSLAYEWLQKGLDWTE p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 p05067pro-mt_nd3 Interaction between polypeptide corresponding to MTND3 and Abeta1-42 identified by biopanning of phage display library. 854138 n/a 854140 unspecified role unspecified role prey prey n/a 854141 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2 mt_nd3-p05067pro Interaction between polypeptide corresponding to MTND3 and Abeta1-42 identified by biopanning of phage display library. 854139 n/a 854140 unspecified role unspecified role bait bait n/a 854141 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2