IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ xie-2006-8 The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. human-22rv1 Homo sapiens 22rv1 Human prostate adenocarcinoma cell line Also known as CWR22Rv1 or Rv1. Cells dsplay a NE (neuroendocrine phenotype). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis This experiment relates to a negative interaction Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer xie-2006-7 The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis This experiment relates to a negative interaction. Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer p11309-1 Serine/threonine-protein kinase pim-1 Pim-1 PIM1 protein protein human Homo sapiens Human MLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK Initiates from CTG codon. bmx_human Cytoplasmic tyrosine-protein kinase BMX Bone marrow tyrosine kinase gene in chromosome X protein Epithelial and endothelial tyrosine kinase NTK38 BMX protein protein human Homo sapiens Human MDTKSILEELLLKRSQQKKKMSPNNYKERLFVLTKTNLSYYEYDKMKRGSRKGSIEIKKIRCVEKVNLEEQTPVERQYPFQIVYKDGLLYVYASNEESRSQWLKALQKEIRGNPHLLVKYHSGFFVDGKFLCCQQSCKAAPGCTLWEAYANLHTAVNEEKHRVPTFPDRVLKIPRAVPVLKMDAPSSSTTLAQYDNESKKNYGSQPPSSSTSLAQYDSNSKKIYGSQPNFNMQYIPREDFPDWWQVRKLKSSSSSEDVASSNQKERNVNHTTSKISWEFPESSSSEEEENLDDYDWFAGNISRSQSEQLLRQKGKEGAFMVRNSSQVGMYTVSLFSKAVNDKKGTVKHYHVHTNAENKLYLAENYCFDSIPKLIHYHQHNSAGMITRLRHPVSTKANKVPDSVSLGNGIWELKREEITLLKELGSGQFGVVQLGKWKGQYDVAVKMIKEGSMSEDEFFQEAQTMMKLSHPKLVKFYGVCSKEYPIYIVTEYISNGCLLNYLRSHGKGLEPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDRDLCVKVSDFGMTRYVLDDQYVSSVGTKFPVKWSAPEVFHYFKYSSKSDVWAFGILMWEVFSLGKQPYDLYDNSQVVLKVSQGHRLYRPHLASDTIYQIMYSCWHELPEKRPTFQQLLSSIEPLREKDKH pim1-bmx-9 PIM1S does not interact with BMX identified by coimmunoprecipitation 218542 N/A 218544 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 218545 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false true 4a BMX associates with PIM1L (UniProt identifier /FTId=PRO_0000043349) but not the shorter isoform PIM1S, prodiced by alternative promoter usage (UniProt identifier /FTId=PRO_0000043350). Author also performs experiments (Fig. 4B) to demonstrate binding of SH3 domain of BMX (ETK) to PIM1L. However, BMX does not contain an SH3 domain and sequence co-ordinates are not given, so this information has not been added. The interaction does not occur under these experimental conditions. pim1-bmx-10 PIM1S does not interact with BMX identified by coimmunoprecipitation 218542 N/A 218544 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 218545 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false true 4a BMX associates with PIM1L (UniProt identifier /FTId=PRO_0000043349) but not the shorter isoform PIM1S, prodiced by alternative promoter usage (UniProt identifier /FTId=PRO_0000043350). Author also performs experiments (Fig. 4B) to demonstrate binding of SH3 domain of BMX (ETK) to PIM1L. However, BMX does not contain an SH3 domain and sequence co-ordinates are not given, so this information has not been added. The interaction does not occur under these experimental conditions. pim1-bmx-8 PIM1S does not interact with BMX identified by coimmunoprecipitation 218543 N/A 218545 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 218544 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false true 4a BMX associates with PIM1L (UniProt identifier /FTId=PRO_0000043349) but not the shorter isoform PIM1S, prodiced by alternative promoter usage (UniProt identifier /FTId=PRO_0000043350). Author also performs experiments (Fig. 4B) to demonstrate binding of SH3 domain of BMX (ETK) to PIM1L. However, BMX does not contain an SH3 domain and sequence co-ordinates are not given, so this information has not been added. The interaction does not occur under these experimental conditions.