IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chipuk-2005-2 PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Science (0036-8075) 2005 chipuk-2005-3 PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Science (0036-8075) 2005 chipuk-2005-4 PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Science (0036-8075) 2005 chipuk-2005-5 PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Science (0036-8075) 2005 chipuk-2005-6 PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Science (0036-8075) 2005 chipuk-2005-1 PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Science (0036-8075) 2005 q64373-1 Bcl-2-like protein 1 Bcl2l1 Bcl2l Bclx Apoptosis regulator Bcl-X Bcl-xL protein protein mouse Mus musculus Mouse MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEAERETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK bbc3_mouse Bcl-2-binding component 3 Bbc3 Puma p53 up-regulated modulator of apoptosis protein protein mouse Mus musculus Mouse MARARQEGSSPEPVEGLARDSPRPFPLGRLMPSAVSCSLCEPGLPAAPAAPALLPAAYLCAPTAPPAVTAALGGPRWPGGHRSRPRGPRPDGPQPSLSPAQQHLESPVPSAPEALAGGPTQAAPGVRVEEEEWAREIGAQLRRMADDLNAQYERRRQEEQHRHRPSPWRVMYNLFMGLLPLPRDPGAPEMEPN p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD q07817-1 Bcl-2-like protein 1 BCLX Apoptosis regulator Bcl-X BCL2L1 BCL2L Bcl-xL protein protein human Homo sapiens Human MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD bbc3_human Bcl-2-binding component 3, isoforms 1/2 p53 up-regulated modulator of apoptosis JFY-1 BBC3 PUMA protein protein human Homo sapiens Human MARARQEGSSPEPVEGLARDGPRPFPLGRLVPSAVSCGLCEPGLAAAPAAPTLLPAAYLCAPTAPPAVTAALGGSRWPGGPRSRPRGPRPDGPQPSLSLAEQHLESPVPSAPGALAGGPTQAAPGVRGEEEQWAREIGAQLRRMADDLNAQYERRRQEEQQRHRPSPWRVLYNLIMGLLPLPRGHRAPEMEPN bax_human Apoptosis regulator BAX BAX BCL2L4 Bcl-2-like protein 4 protein protein human Homo sapiens Human MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG tp53-bbc3-2 Interaction between murine TP53, BCL-XL and BBC3 (PUMA) identified by coimmunoprecipitation 252028 N/A 252034 unspecified role unspecified role bait bait N/A 252035 unspecified role unspecified role prey prey N/A 252036 unspecified role unspecified role prey prey association association false false false tp53-bbc3-4 Interaction between murine TP53, BCL-XL and BBC3 (PUMA) identified by coimmunoprecipitation 252028 N/A 252036 unspecified role unspecified role prey prey N/A 252037 unspecified role unspecified role bait bait N/A 252035 unspecified role unspecified role prey prey association association false false false tp53-bclxl-5 Interaction between murine TP53, and human BCL-XL identified by coimmunoprecipitation 252028 N/A 252037 unspecified role unspecified role bait bait N/A 252036 unspecified role unspecified role prey prey physical association physical association false false false BBC3 -/- cells used as host organism tp53-bbc3-5 Interaction between murine BBC3 (PUMA) and human BCL-XLidentified by coimmunoprecipitation 252028 N/A 252037 unspecified role unspecified role bait bait Q07817-1:p.Gly138Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false N/A 252035 unspecified role unspecified role prey prey physical association physical association false false false tp53-bclxl-2 Interaction between TP53 and BCL-XL identified by coimmunoprecipitation 252029 N/A 252037 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 252038 unspecified role unspecified role prey prey physical association physical association false false false BBC3(PUMA) BH3 peptide tp53-bclxl-4 Interaction between TP53 and BCL-XL identified by coimmunoprecipitation 252029 N/A 252039 unspecified role unspecified role prey prey N/A 252037 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q07817-1:p.Gly138Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false bbc3-bclxl-4 Interaction of BBC3 (PUMA) with BCL-xl by biacore 252030 N/A 252037 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 252039 unspecified role unspecified role prey prey direct interaction direct interaction false false false tp53-bclxl-3 Interaction of p53 with BCL-xl by biacore 252030 N/A 252037 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 252038 unspecified role unspecified role prey prey direct interaction direct interaction false false false tp53-bbc3-3 Interaction between human BCL-XL and murine BBC3 (PUMA) identified by coimmunoprecipitation 252031 N/A 252037 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 252035 unspecified role unspecified role prey prey physical association physical association false false false BBC3+/+ cells tp53-bclxl-1 Interaction between murine TP53 and BCL-XL identified by coimmunoprecipitation 252031 N/A 252037 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 252036 unspecified role unspecified role prey prey physical association physical association false false false BBC3 (PUMA)-/- cells bbc3-bclxl-5 Interaction of BBC3 (PUMA) with BCL-xl by coimmunoprecipitation 252032 N/A 252039 unspecified role unspecified role prey prey N/A 252037 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q07817-1:p.Gly138Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false bax-bclxl-1 Interaction of BAX with BCL-xl by coimmunoprecipitation 252032 N/A 252040 unspecified role unspecified role prey prey N/A 252037 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q07817-1:p.Gly138Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false tp53-bbc3-1 Interaction between murine TP53, BCL-XL and BBC3 (PUMA) identified by coimmunoprecipitation 252033 N/A 252034 unspecified role unspecified role bait bait N/A 252035 unspecified role unspecified role prey prey N/A 252036 unspecified role unspecified role prey prey association association false false false