IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kang-2006-3 AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kang CH., Jung WY., Kang YH., Kim JY., Kim DG., Jeong JC., Baek DW., Jin JB., Lee JY., Kim MO., Chung WS., Mengiste T., Koiwa H., Kwak SS., Bahk JD., Lee SY., Nam JS., Yun DJ., Cho MJ. Apoptosis - Interactions involving proteins with a function related to apoptosis The CaM::HRP overlay assay was performed in the absence (5mM EGTA) or presence (1mM CaCl2)of CaCl2. The interacting proteins were detected by enhanced chemiluminescent" (ECL) detection method. djyun@gsnu.ac.kr A cDNA library of 4-week-old A. thaliana (ecotype Columbia) plants that were treated with heat shock (37deree celsius) for 2 h. Cell Death Differ. (1350-9047) 2006 Only protein-protein interactions imex curation kang-2006-1 AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting immunostaining immunostaining Kang CH., Jung WY., Kang YH., Kim JY., Kim DG., Jeong JC., Baek DW., Jin JB., Lee JY., Kim MO., Chung WS., Mengiste T., Koiwa H., Kwak SS., Bahk JD., Lee SY., Nam JS., Yun DJ., Cho MJ. Apoptosis - Interactions involving proteins with a function related to apoptosis The CaM::HRP overlay assay was performed in the absence (5mM EGTA) or presence (1mM CaCl2)of CaCl2. The interacting proteins were detected by enhanced chemiluminescent" (ECL) detection method. djyun@gsnu.ac.kr Cell Death Differ. (1350-9047) 2006 Only protein-protein interactions imex curation kang-2006-2 AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kang CH., Jung WY., Kang YH., Kim JY., Kim DG., Jeong JC., Baek DW., Jin JB., Lee JY., Kim MO., Chung WS., Mengiste T., Koiwa H., Kwak SS., Bahk JD., Lee SY., Nam JS., Yun DJ., Cho MJ. Apoptosis - Interactions involving proteins with a function related to apoptosis djyun@gsnu.ac.kr Cell Death Differ. (1350-9047) 2006 Only protein-protein interactions imex curation The CaM::HRP overlay assay was performed in the absence (5mM EGTA) or presence (1mM CaCl2)of CaCl2. The interacting proteins were detected by enhanced chemiluminescent" (ECL) detection method. calm2_arath Calmodulin-2/3/5 CAM2 CAL1 At2g41110 T3K9.12 CAM3 At3g56800 T8M16.130 CAM5 TCH1 At2g27030 T20P8.8 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEADVDGDGQINYEEFVKVMMAK bag6_arath BAG family molecular chaperone regulator 6 BAG6 Bcl-2-associated athanogene 6 CAMBP1 CaM-binding protein 1 T3F17.11 At2g46240 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMPVYMDPSQPCQMRPQEYYYQGFGNNSQHMAMDAPPPCHGSCVHGNFPAYWPPCYPPQVPYHQCCMNRSAFHPPHASYAPSCYVHPPFPVGYQPWFDVEKDVPGKHHCGKCSSQMCDLKKDRGVVIEEHEPEIEKGEAVLPVRSTNCPYPIIWIPHENARNQEYRSSLGLGKHNQPPAEVRAPDNMTIQKSFPESWRGCFPFDESSMKSLVQNQDSKKAQNGKTVEAPFDISKFKSLLQGQDMKEAQIQKNKEELGQLTYPTSWVPSRRKRDDVEASESSNEDRKKMQNGKTVEYPFDISMIKSLIQGQDVKEAQNQKNKEEPGQVPYPIFWIPSYGKRKDVEASESKESSNEGRNLESCPSDLHRNEGQITQAKGKEGNFECNVLSDAEEKSSVINIPVANHLQEPRNIPVKLSENHLPKPTEPTKRIAKNEPVKSTKKEQSSSSSEASKLPPVCLRVDPLPKERNGGSKSVSHPKRMEKSKETKIAAPLSSKKAESRTVPEACNVKCEDANAEMKMAEGSLNALRTEKGSVESNSNLQEESNGEIIKPCEAKENREQPAKKSFTEEEAARIIQSMYRGYDVRRWEPIKKLKEIATVREQMGDVKKRIEALEASTDQHIEEKEIVVNGELVMNLLLKLDAVEGLHPSIREFRKALATELSSIQDKLDSLKNSCASAEKEAVKEQVEIKSQPSDSPVNLEHSQLTEENKMVSDTNLEKVLRLSPEEHPMSVLNRTDEKQAESAAETEEGYGLFETLATDSKQATENAAAASSTTIPEKIGEVETVVPGNPPSADGNGMTVTNVEENKAMVVESLEEPINELPQMVEETETNSIRDPENASEVSEAETNSSENENRKGEDDIVLHSEKNVELSELPVGVIDEETQPLSQDPSSSYTREGNMTAMDPKTASQEETEVDHSPNNSKGIGQQTSEPQDEKEQSPETEVIVKEQPLETEVILNEQAPEPEITEPGISKETKKLMEENQRFKETMETLVKAGREQLEVISKLTSRVKSLEKKLSHKKKTQIRRRASKPMSVSPTDAVL calm1_arath Calmodulin-1/4 CAM1 At5g37780 K22F20.2 T31G3.3 CAM4 At1g66410 T27F4.1 F28G11.13 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIREADVDGDGQINYEEFVKIMMAK calm6_arath Calmodulin-6 CAM6 F13M11 At5g21274 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDEEVDEMIREADVDGDGQINYEEFVKVMMAK calm7_arath Calmodulin-7 CAM7 T28A8.100 At3g43810 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFVKVMMAK hs701_arath Heat shock 70 kDa protein 1 HSP70-1 ERD2 HSC70-1 Heat shock cognate 70 kDa protein 1 Heat shock cognate protein 70-1 Heat shock protein 70-1 MED37E MED37_4 Protein EARLY-RESPONSIVE TO DEHYDRATION 2 T22P11.90 At5g02500 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPVNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQAGPADKPMIYVEYKGEEKEFAAEEISSMVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQGAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTTLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKDEIEKMVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIQDEKIGEKLPAADKKKIEDSIEQAIQWLEGNQLAEADEFEDKMKELESICNPIIAKMYQGAGGEAGGPGASGMDDDAPPASGGAGPKIEEVD bag3_arath BAG family molecular chaperone regulator 3 BAG3 Bcl-2-associated athanogene 3 T28J14_160 At5g07220 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMKMNTGTSPSVIGGGTSGNEWESRPGGMVVQRRTDQNSDVPRVFRVRVKYGSVYHEININSQSSFGELKKMLSDQVGLHHEDMKVLYKDKERDSKMFLDLCGVKDRSKLVVKEDPISQEKRLLAKRKNAAIEKASKSISDISFEVDRLAGQVSAFETVINKGGKVEEKSLVNLIEMLMNQLLRLDAIIADGDVKLMRKMQVQRVQKYVEALDLLKVKNSAKKVEVNKSVRHKPQTQTRFEQRDLLSFVEEEEEEPRNSNASSSSGTPAVVASKWEMFDSASTAKAAETVKPVPPRFKWEFFD bag5_arath BAG family molecular chaperone regulator 5, mitochondrial BAG5 Bcl-2-associated athanogene 5 F12F1.7 At1g12060 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKRSRKFSSSTTTTTVIHTFYNDHTTPPATKEIPIETPLPATKANVKTNATAAAARIQSGYRSYRIRNLYKKISSINREANRVQSIIQRQETVDAIRSDEKERLRMNETLMALLLKLDSVPGLDPTIREARRKVSRKIVGMQEILDSISETKDEIQWWNYNDLGGVDSGQGGGAWPLYWEEAVEEEMCRERGGEEMERFCAQYLGFRCFQRFLRE p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD lt_sv40 Large T antigen protein protein sv40 sv40 SV40 MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET bag6-cam2-2 Interaction between BAG3 protein and Cam2 protein 667003 N/A 667006 unspecified role unspecified role prey prey N/A 667007 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false bag6-cam1 Interaction between BAG3 protein and Cam1 protein 667003 N/A 667007 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 667008 unspecified role unspecified role prey prey physical association physical association false false false bag6-cam3 Interaction between BAG3 protein and Cam3 protein 667003 N/A 667007 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 667006 unspecified role unspecified role prey prey physical association physical association false false false bag6-cam4 Interaction between BAG3 protein and Cam4 protein 667003 N/A 667007 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 667008 unspecified role unspecified role prey prey physical association physical association false false false bag6-cam6 Interaction between BAG3 protein and Cam6 protein 667003 N/A 667007 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 667009 unspecified role unspecified role prey prey physical association physical association false false false bag6-cam7 Interaction between BAG3 protein and Cam7 protein 667003 N/A 667007 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 667010 unspecified role unspecified role prey prey physical association physical association false false false bag6-cam2 Interaction between BAG3 protein and Cam2 protein 667004 N/A 667006 unspecified role unspecified role prey prey region hrp tag horseradish peroxidase tag hrp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 667007 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false O82345:p.Ile575Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false O82345:p.Ile575Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 2, Figure 3 The interaction was inhibited by the presence of CaCl (calcium chloride). bag3-hsp70 Interaction between BAG3 protein and Heat shock cognate 70 kDa protein 1 667004 N/A 667011 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 667012 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 7 bag5-hsp70 Interaction between BAG5 protein and Heat shock cognate 70 kDa protein 1 667004 N/A 667011 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 667013 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 7 bag6-cam2-1 Interaction between BAG3 protein and Cam2 protein 667005 N/A 667007 unspecified role unspecified role bait bait O82345:p.Ile575Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false O82345:p.Ile575Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false N/A 667006 unspecified role unspecified role prey prey physical association physical association false false false Figure 3 B p53-sv40t-3 Interaction between p53 and SV40 Large T antigen 667005 N/A 667014 unspecified role unspecified role prey prey N/A 667015 unspecified role unspecified role bait bait physical association physical association false false false 3B