UniProt UniProt http://www.uniprot.org kurki-2004-6 Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Peltonen K., Latonen L., Kiviharju TM., Ojala PM., Meek D., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 2b marikki.laiho@helsinki.fi Cancer Cell (1535-6108) 2004 kurki-2004-1 Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Peltonen K., Latonen L., Kiviharju TM., Ojala PM., Meek D., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 2b marikki.laiho@helsinki.fi Cancer Cell (1535-6108) 2004 kurki-2004-2 Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kurki S., Peltonen K., Latonen L., Kiviharju TM., Ojala PM., Meek D., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 2b marikki.laiho@helsinki.fi Cancer Cell (1535-6108) 2004 kurki-2004-3 Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Peltonen K., Latonen L., Kiviharju TM., Ojala PM., Meek D., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 2b marikki.laiho@helsinki.fi Cancer Cell (1535-6108) 2004 kurki-2004-4 Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Peltonen K., Latonen L., Kiviharju TM., Ojala PM., Meek D., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 2b marikki.laiho@helsinki.fi Cancer Cell (1535-6108) 2004 kurki-2004-5 Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. mouse-foetal fibroblast Mus musculus fetal fibroblast foetal_fibroblast Fibroblasts from foetal tissue Fibroblasts from a greater than 15.5 dpc foetus Fibroblasts from a foetus as apposed to an embryonic or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Peltonen K., Latonen L., Kiviharju TM., Ojala PM., Meek D., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 2b marikki.laiho@helsinki.fi Cancer Cell (1535-6108) 2004 kurki-2004-7 Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Peltonen K., Latonen L., Kiviharju TM., Ojala PM., Meek D., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 2b marikki.laiho@helsinki.fi Cancer Cell (1535-6108) 2004 kurki-2004-8 Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Peltonen K., Latonen L., Kiviharju TM., Ojala PM., Meek D., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis fig 2b marikki.laiho@helsinki.fi Cancer Cell (1535-6108) 2004 kurki-2004-9 Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Peltonen K., Latonen L., Kiviharju TM., Ojala PM., Meek D., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cells were transfected with a SUMO1 expression construct. fig 2b marikki.laiho@helsinki.fi Cancer Cell (1535-6108) 2004 kurki-2004-10 Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Peltonen K., Latonen L., Kiviharju TM., Ojala PM., Meek D., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cells were transfected with Kaposi's sarcoma-associated herpesvirus cyclin (cyclin K). fig 2b marikki.laiho@helsinki.fi Cancer Cell (1535-6108) 2004 kurki-2004-11 Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kurki S., Peltonen K., Latonen L., Kiviharju TM., Ojala PM., Meek D., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cells were transfected with Kaposi's sarcoma-associated herpesvirus cyclin (cyclin K). fig 2b marikki.laiho@helsinki.fi Cancer Cell (1535-6108) 2004 p06748-2 Nucleophosmin NPM1 NPM Nucleolar phosphoprotein B23 Nucleolar protein NO38 Numatrin protein protein human Homo sapiens Human MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKLAADEDDDDDDEEDDDEDDDDDDFDDEEAEEKAPVKKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL p06748-1 Nucleophosmin NPM1 NPM Nucleolar phosphoprotein B23 Nucleolar protein NO38 Numatrin protein protein human Homo sapiens Human MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKLAADEDDDDDDEEDDDEDDDDDDFDDEEAEEKAPVKKSIRDTPAKNAQKSNQNGKDSKPSSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP npm_human Nucleophosmin NPM1 NPM Nucleolar phosphoprotein B23 Numatrin Nucleolar protein NO38 protein protein human Homo sapiens Human MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKLAADEDDDDDDEEDDDEDDDDDDFDDEEAEEKAPVKKSIRDTPAKNAQKSNQNGKDSKPSSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL npm_mouse Nucleophosmin Nucleolar phosphoprotein B23 Numatrin Nucleolar protein NO38 Npm1 protein protein mouse Mus musculus Mouse MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEDEEDVKLLGMSGKRSAPGGGNKVPQKKVKLDEDDEDDDEDDEDDEDDDDDDFDEEETEEKVPVKKSVRDTPAKNAQKSNQNGKDLKPSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL q98147_hhv8 protein protein hhv8 Human herpesvirus 8 (HHV-8) Kaposi's sarcoma-associated herpesvirus HHV-8 MATANNPPSGLLDPTLCEDRIFYNILEIEPRFLTSDSVFGTFQQSLTSHMRKLLGTWMFSVCQEYNLEPNVVALALNLLDRLLLIKQVSKEHFQKTGSACLLVASKLRSLTPISTSSLCYAAADSFSRQELIDQEKELLEKLAWRTEAVLATDVTSFLLLKLVGGSQHLDFWHHEVNTLITKALVDPKTGSLPASIISAAGCALLVPANVIPQDTHSGGVVPQLASILGCDVSVLQAAVEQILTSVSDFDLRILDSY tp53-npm1-2 coIP: bait = p53; prey = NPM1 204735 N/A 204746 unspecified role unspecified role prey prey N/A 204747 unspecified role unspecified role prey prey N/A 204748 unspecified role unspecified role bait bait association association false false false Interaction is stimulated by transfection with Kaposi's sarcoma virus cyclin homolog (cyclin K). mdm2-npm1-6 coIP: bait = HDM2; preys = NPM1 and p53 204735 N/A 204749 unspecified role unspecified role bait bait N/A 204748 unspecified role unspecified role prey prey N/A 204746 unspecified role unspecified role prey prey N/A 204747 unspecified role unspecified role prey prey association association false false false NPM1 negatively regulates the MDM2-TP53 interaction. MDM2-NPM1 interaction is stimulated by transfection with Kaposi's sarcoma virus cyclin homolog (cyclin K). mdm2-npm1-1 coIP: bait = HDM2, prey = NPM 204736 N/A 204749 unspecified role unspecified role bait bait N/A 204750 unspecified role unspecified role prey prey physical association physical association false false false mdm2-npm1-2 GST pull down: bait = HDM2, prey = NPM 204737 N/A 204749 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 204750 unspecified role unspecified role prey prey 35s radiolabel tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association false false false mdm2-npm1-3 coIP: bait = HDM2, prey = NPM 204738 N/A 204751 unspecified role unspecified role prey prey mouse-foetal fibroblast Mus musculus fetal fibroblast foetal_fibroblast Fibroblasts from foetal tissue Fibroblasts from a greater than 15.5 dpc foetus Fibroblasts from a foetus as apposed to an embryonic or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. N/A 204749 unspecified role unspecified role bait bait rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association false false false Inhibited by high levels of TP53 protein. Stimulated by low levels of TP53 protein. mdm2-npm1-4 coIP: bait = HDM2, prey = NPM 204739 N/A 204749 unspecified role unspecified role bait bait N/A 204750 unspecified role unspecified role prey prey physical association physical association false false false Transiently stimulated by irradiation with UVC. mdm2-npm1-5 coIP: bait = HDM2, prey = NPM 204740 N/A 204751 unspecified role unspecified role prey prey N/A 204749 unspecified role unspecified role bait bait physical association physical association false false false By irradiation with UVC. npm1-tp53-2 coIP: bait = npm1; prey =p53 204741 N/A 204750 unspecified role unspecified role bait bait N/A 204748 unspecified role unspecified role prey prey physical association physical association false false false Transient stimulation by irradiation with UVC. npm1-tp53-3 coIP: bait = npm1; prey =p53 204742 N/A 204748 unspecified role unspecified role prey prey N/A 204750 unspecified role unspecified role bait bait physical association physical association false false false Transient stimulation by irradiation with UVC. npm1-tp53-4 coIP: bait = npm1; prey =p53 204743 N/A 204750 unspecified role unspecified role bait bait N/A 204748 unspecified role unspecified role prey prey physical association physical association false false false Sumoylation of TP53. orf72-npm1-1 coIP: bait = NPM1; prey = KSHV cyclin (cyclin k) 204744 N/A 204752 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 204750 unspecified role unspecified role bait bait physical association physical association false false false npm1-orf72-2 coIP: bait = KSHV cyclin (cyclin k); prey = NPM1 204745 N/A 204752 unspecified role unspecified role bait bait n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 204750 unspecified role unspecified role prey prey physical association physical association false false false