IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tinel-2004-1 The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Tinel A., Tschopp J. Apoptosis - Interactions involving proteins with a function related to apoptosis Science (0036-8075) 2004 tinel-2004-2 The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress. human-jurkat Homo sapiens jurkat Human T cell leukemia affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Tinel A., Tschopp J. Apoptosis - Interactions involving proteins with a function related to apoptosis Biotin-VAD-fmk can immunoprecipitates active caspases using streptavidin beads. Under the conditions used, it was shown the only caspase immunoprecipitated was caspase 2 Science (0036-8075) 2004 tinel-2004-3 The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Tinel A., Tschopp J. Apoptosis - Interactions involving proteins with a function related to apoptosis size-exlusion chromatography of Hela cells cytoplasmic extracts Science (0036-8075) 2004 cradd_human Death domain-containing protein CRADD Caspase and RIP adapter with death domain RIP-associated protein with a death domain CRADD RAIDD protein protein human Homo sapiens Human MEARDKQVLRSLRLELGAEVLVEGLVLQYLYQEGILTENHIQEINAQTTGLRKTMLLLDILPSRGPKAFDTFLDSLQEFPWVREKLKKAREEAMTDLPAGDRLTGIPSHILNSSPSDRQINQLAQRLGPEWEPMVLSLGLSQTDIYRCKANHPHNVQSQVVEAFIRWRQRFGKQATFQSLHNGLRAVEVDPSLLLHMLE casp2_human Caspase-2 Protease ICH-1 Neural precursor cell expressed developmentally down-regulated protein 2 CASP2 ICH1 NEDD2 protein protein human Homo sapiens Human MAAPSAGSWSTFQHKELMAADRGRRILGVCGMHPHHQETLKKNRVVLAKQLLLSELLEHLLEKDIITLEMRELIQAKVGSFSQNVELLNLLPKRGPQAFDAFCEALRETKQGHLEDMLLTTLSGLQHVLPPLSCDYDLSLPFPVCESCPLYKKLRLSTDTVEHSLDNKDGPVCLQVKPCTPEFYQTHFQLAYRLQSRPRGLALVLSNVHFTGEKELEFRSGGDVDHSTLVTLFKLLGYDVHVLCDQTAQEMQEKLQNFAQLPAHRVTDSCIVALLSHGVEGAIYGVDGKLLQLQEVFQLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHAGSPGCEESDAGKEKLPKMRLPTRSDMICGYACLKGTAAMRNTKRGSWYIEALAQVFSERACDMHVADMLVKVNALIKDREGYAPGTEFHRCKEMSEYCSTLCRHLYLFPGHPPT pidd1_human p53-induced death domain-containing protein 1 PIDD1 LRDD Leucine-rich repeat and death domain-containing protein PIDD protein protein human Homo sapiens Human MAATVEGPELEAAAAAGDASEDSDAGSRALPFLGGNRLSLDLYPGGCQQLLHLCVQQPLQLLQVEFLRLSTHEDPQLLEATLAQLPQSLSCLRSLVLKGGQRRDTLGACLRGALTNLPAGLSGLAHLAHLDLSFNSLETLPACVLQMRGLGALLLSHNCLSELPEALGALPALTFLTVTHNRLQTLPPALGALSTLQRLDLSQNLLDTLPPEIGGLGSLLELNLASNRLQSLPASLAGLRSLRLLVLHSNLLASVPADLARLPLLTRLDLRDNQLRDLPPELLDAPFVRLQGNPLGEASPDAPSSPVAALIPEMPRLFLTSDLDSFPVTPQGCSVTLACGVRLQFPAGATATPITIRYRLLLPEPGLVPLGPHDALLSHVLELQPHGVAFQQDVGLWLLFTPPQARRCREVVVRTRNDNSWGDLETYLEEEAPQRLWAHCQVPHFSWFLVVSRPVSNACLVPPEGTLLCSSGHPGVKVIFPPGATEEPRRVSMQVVRMAGRELQALLGEPEAAVSPLLCLSQSGPPSFLQPVTVQLPLPSGITGLSLDRSRLHLLYWAPPAATWDDITAQVVLELTHLYARFQVTHFSWYWLWYTTKNCVGGLARKAWERLRLHRVNLIALQRRRDPEQVLLQCLPRNKVDATLRRLLERYRGPEPSDTVEMFEGEEFFAAFERGIDVDADRPDCVEGRICFVFYSHLKNVKEVYVTTTLDREAQAVRGQVSFYRGAVPVRVPEEAEAARQRKGADALWMATLPIKLPRLRGSEGPRRGAGLSLAPLNLGDAETGFLTQSNLLSVAGRLGLDWPAVALHLGVSYREVQRIRHEFRDDLDEQIRHMLFSWAERQAGQPGAVGLLVQALEQSDRQDVAEEVRAVLELGRRKYQDSIRRMGLAPKDPALPGSSAPQPPEPAQA casp2-cradd-1 In vivo Co-IP: bait= human caspase 2, prey = human RAIDD 174655 N/A 174658 unspecified role unspecified role prey prey n-terminal vsv tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 174659 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false card domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Both proteins are overexpressed lrdd-cradd-1 In vivo Co-IP: bait= PIDD, prey = death domain of RAIDD 174655 N/A 174658 unspecified role unspecified role prey prey n-terminal vsv tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false death domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 174660 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false casp2-cradd-3 In vivo Co-IP: bait= human caspase 2, prey = human RAIDD, prey= PIDD 174655 N/A 174659 unspecified role unspecified role bait bait card domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 174660 unspecified role unspecified role prey prey n-terminal vsv tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 174658 unspecified role unspecified role prey prey n-terminal vsv tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false All proteins are overexpressed PIDD is co-immunoprecipitated with caspase 2 only if RAIDD is overexpressed casp2-cradd-5 In vivo Co-IP: bait= PIDD, prey = RAIDD, prey= caspase 2 174655 N/A 174658 unspecified role unspecified role prey prey N/A 174659 unspecified role unspecified role prey prey N/A 174660 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false immuoprecipitated caspase 2 can be found in several form: pro-caspase-2, p31, p19 casp2-cradd-4 In vivo Co-IP: bait= human caspase 2, prey = human RAIDD, prey= PIDD 174656 N/A 174659 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 174660 unspecified role unspecified role prey prey n-terminal vsv tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 174658 unspecified role unspecified role prey prey n-terminal vsv tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false all proteins are endogenous casp2-cradd-6 In vivo Co-IP: bait= human caspase 2, prey = human RAIDD 174657 N/A 174659 unspecified role unspecified role neutral component neutral component N/A 174658 unspecified role unspecified role neutral component neutral component physical association physical association false false false caspase 2 and RAIDD co-eluted in fraction coorresponding to a molecular mass of 670 kDa