IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kashuba-2003-4 EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. human-m1 Homo sapiens fibroblast cell line m1 M1 human fibrobast murine myeloid cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of p14ARF taken from PMID 10772813 elena.kashuba@mtc.ki.se Int. J. Cancer (0020-7136) 2003 Cancer - Interactions investigated in the context of cancer kashuba-2003-1 EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis elena.kashuba@mtc.ki.se Int. J. Cancer (0020-7136) 2003 Cancer - Interactions investigated in the context of cancer kashuba-2003-2 EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of protein p14ARF taken from PMID 10772813 elena.kashuba@mtc.ki.se Int. J. Cancer (0020-7136) 2003 Cancer - Interactions investigated in the context of cancer kashuba-2003-3 EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Kashuba E., Mattsson K., Pokrovskaja K., Kiss C., Protopopova M., Ehlin-Henriksson B., Klein G., Szekely L. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of p14ARF taken from PMID 10772813 elena.kashuba@mtc.ki.se Int. J. Cancer (0020-7136) 2003 Cancer - Interactions investigated in the context of cancer arf_human Tumor suppressor ARF p14ARF CDKN2A CDKN2 MLM Alternative reading frame Cyclin-dependent kinase inhibitor 2A protein protein human Homo sapiens Human MVRRFLVTLRIRRACGPPRVRVFVVHIPRLTGEWAAPGAPAAVALVLMLLRSQRLGQQPLPRRPGHDDGQRPSGGAAAAPRRGAQLRRPRHSHPTRARRCPGGLPGHAGGAAPGRGAAGRARCLGPSARGPG pml_human Protein PML PML MYL RNF71 TRIM19 Tripartite motif-containing protein 19 RING finger protein 71 Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNETQKISQLAAVNRESKFRVVIQPEAFFSIYSKAVSLEVGLQHFLSFLSSMRRPILACYKLWGPGLPNFFRALEDINRLWEFQEAISGFLAALPLIRERVPGASSFKLKNLAQTYLARNMSERSAMAAVLAMRDLCRLLEVSPGPQLAQHVYPFSSLQCFASLQPLVQAAVLPRAEARLLALHNVSFMELLSAHRRDRQGGLKKYSRYLSLQTTTLPPAQPAFNLQALGTYFEGLLEGPALARAEGVSTPLAGRGLAERASQQS mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD ebna5_ebvb9 Epstein-Barr nuclear antigen leader protein EBNA-LP EBNA5 Epstein-Barr nuclear antigen 5 protein protein ebvb9 Epstein-Barr virus (strain B95-8) (HHV-4) Human herpesvirus 4 HHV-4 MGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPRGDRSEGPGPTRPGPPGIGPEGPLGQLLRRHRSPSPTRGGQEPRRVRRRVLVQQEEEVVSGSPSGPLRPRPRPPARSLREWLLRIRDHFEPPTVTTQRQSVYIEEEEDED cdkn2a-pml p14ARF localizes to PML bodies 576086 N/A 576090 unspecified role unspecified role neutral component neutral component N/A 576091 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 5 A Q8N726 transfection cdkn2a-mdm2 p14ARF colocalizes with HDM2 in M1 cells 576086 N/A 576090 unspecified role unspecified role neutral component neutral component N/A 576092 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 5 C Q8N726 transfection cdkn2a-tp53 p14ARF colocalizes with p53 in M1 cells 576086 N/A 576090 unspecified role unspecified role neutral component neutral component N/A 576093 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 5 D Q8N726 transfection cdkn2a-tp53-1 p14ARF colocalizes with p53 in M1 cells 576086 N/A 576090 unspecified role unspecified role neutral component neutral component N/A 576094 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4 C Q8N726 transfection cdkn2a-ebna_lp In a yeast two-hybrid assay p14ARF interacted with EBNA-5 576087 N/A 576090 unspecified role unspecified role bait bait N/A 576094 unspecified role unspecified role prey prey physical association physical association false false false 1 The EBNA-LP protein referred to by UniprotKB accession number Q8AZK7 is from strain GD1 of EBV. However, the authors do not specify which strain their construct comes from. ebna_lp-cdkn2a In a yeast two-hybrid assay p14ARF interacted with EBNA-5 576087 N/A 576090 unspecified role unspecified role prey prey N/A 576094 unspecified role unspecified role bait bait physical association physical association false false false 1 The EBNA-LP protein referred to by UniprotKB accession number Q8AZK7 is from strain GD1 of EBV. However, the authors do not specify which strain their construct comes from. cdkn2a-ebna_lp-1 GST-2TK-p14ARF pulls down EBNA-5 from SW480 cell lysates 576088 N/A 576090 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 576094 unspecified role unspecified role prey prey human-colon human colon colon physical association physical association false false false 2 The EBNA-LP protein referred to by Uniprotkb accession number Q8AZK7 is from strain GD1 of EBV. However, the authors do not specify which strain their construct comes from. cdkn2a-ebna_lp-2 GFP-p14ARF and EBNA-5 colocalize in MCF7 cells 576089 N/A 576090 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 576094 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false The EBNA-LP protein referred to by Uniprotkb accession number Q8AZK7 is from strain GD1 of EBV. However, the authors do not specify which strain their construct comes from. 4 A Q8N726; Q8AZK7 transfection