UniProt UniProt http://www.uniprot.org ugai-2001-5 Interaction of Myc-associated zinc finger protein with DCC, the product of a tumor-suppressor gene, during the neural differentiation of P19 EC cells. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ugai H., Li HO., Komatsu M., Tsutsui H., Song J., Shiga T., Fearon E., Murata T., Yokoyama KK. Apoptosis - Interactions involving proteins with a function related to apoptosis P19 EC cells have been treated with RA (retinoic acid). kazu@rtc.riken.go.jp Biochem. Biophys. Res. Commun. (0006-291X) 2001 ugai-2001-1 Interaction of Myc-associated zinc finger protein with DCC, the product of a tumor-suppressor gene, during the neural differentiation of P19 EC cells. mouse-p19 Mus musculus embryonic teratocarcinoma cell line, epithelial-like, induced by retinoic acid p19 mouse embryonic teratocarcinoma cellline anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Ugai H., Li HO., Komatsu M., Tsutsui H., Song J., Shiga T., Fearon E., Murata T., Yokoyama KK. Apoptosis - Interactions involving proteins with a function related to apoptosis P19 EC cells have been treated with RA (retinoic acid). kazu@rtc.riken.go.jp Biochem. Biophys. Res. Commun. (0006-291X) 2001 ugai-2001-3 Interaction of Myc-associated zinc finger protein with DCC, the product of a tumor-suppressor gene, during the neural differentiation of P19 EC cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ugai H., Li HO., Komatsu M., Tsutsui H., Song J., Shiga T., Fearon E., Murata T., Yokoyama KK. Apoptosis - Interactions involving proteins with a function related to apoptosis P19 EC cells have been treated with RA (retinoic acid). kazu@rtc.riken.go.jp Biochem. Biophys. Res. Commun. (0006-291X) 2001 ugai-2001-2 Interaction of Myc-associated zinc finger protein with DCC, the product of a tumor-suppressor gene, during the neural differentiation of P19 EC cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ugai H., Li HO., Komatsu M., Tsutsui H., Song J., Shiga T., Fearon E., Murata T., Yokoyama KK. Apoptosis - Interactions involving proteins with a function related to apoptosis P19 EC cells have been treated with RA (retinoic acid). kazu@rtc.riken.go.jp cDNA from P19 EC cells treated with RA (retinoic acid). Biochem. Biophys. Res. Commun. (0006-291X) 2001 ugai-2001-4 Interaction of Myc-associated zinc finger protein with DCC, the product of a tumor-suppressor gene, during the neural differentiation of P19 EC cells. mouse-p19 Mus musculus embryonic teratocarcinoma cell line, epithelial-like, induced by retinoic acid p19 mouse embryonic teratocarcinoma cellline anti tag coip anti tag coimmunoprecipitation anti tag coip antibody detection identification by antibody antibody detection Ugai H., Li HO., Komatsu M., Tsutsui H., Song J., Shiga T., Fearon E., Murata T., Yokoyama KK. Apoptosis - Interactions involving proteins with a function related to apoptosis P19 EC cells have been treated with RA (retinoic acid). kazu@rtc.riken.go.jp Biochem. Biophys. Res. Commun. (0006-291X) 2001 ugai-2001-6 Interaction of Myc-associated zinc finger protein with DCC, the product of a tumor-suppressor gene, during the neural differentiation of P19 EC cells. mouse-p19 Mus musculus embryonic teratocarcinoma cell line, epithelial-like, induced by retinoic acid p19 mouse embryonic teratocarcinoma cellline confocal microscopy confocal microscopy immunostaining immunostaining Ugai H., Li HO., Komatsu M., Tsutsui H., Song J., Shiga T., Fearon E., Murata T., Yokoyama KK. Apoptosis - Interactions involving proteins with a function related to apoptosis P19 EC cells have been treated with RA (retinoic acid). kazu@rtc.riken.go.jp Biochem. Biophys. Res. Commun. (0006-291X) 2001 maz_human Myc-associated zinc finger protein Transcription factor Zif87 Zinc finger protein 801 MAZ Purine-binding transcription factor Pur-1 ZF87 ZNF801 Serum amyloid A-activating factor-1 protein protein human Homo sapiens Human MFPVFPCTLLAPPFPVLGLDSRGVGGLMNSFPPPQGHAQNPLQVGAELQSRFFASQGCAQSPFQAAPAPPPTPQAPAAEPLQVDLLPVLAAAQESAAAAAAAAAAAAAVAAAPPAPAAASTVDTAALKQPPAPPPPPPPVSAPAAEAAPPASAATIAAAAATAVVAPTSTVAVAPVASALEKKTKSKGPYICALCAKEFKNGYNLRRHEAIHTGAKAGRVPSGAMKMPTMVPLSLLSVPQLSGAGGGGGEAGAGGGAAAVAAGGVVTTTASGKRIRKNHACEMCGKAFRDVYHLNRHKLSHSDEKPYQCPVCQQRFKRKDRMSYHVRSHDGAVHKPYNCSHCGKSFSRPDHLNSHVRQVHSTERPFKCEKCEAAFATKDRLRAHTVRHEEKVPCHVCGKMLSSAYISDHMKVHSQGPHHVCELCNKGTGEVCPMAAAAAAAAAAAAAAVAAPPTAVGSLSGAEGVPVSSQPLPSQPW dcc_human Netrin receptor DCC DCC Tumor suppressor protein DCC Colorectal cancer suppressor IGDCC1 Immunoglobulin superfamily DCC subclass member 1 protein protein human Homo sapiens Human MENSLRCVWVPKLAFVLFGASLFSAHLQVTGFQIKAFTALRFLSEPSDAVTMRGGNVLLDCSAESDRGVPVIKWKKDGIHLALGMDERKQQLSNGSLLIQNILHSRHHKPDEGLYQCEASLGDSGSIISRTAKVAVAGPLRFLSQTESVTAFMGDTVLLKCEVIGEPMPTIHWQKNQQDLTPIPGDSRVVVLPSGALQISRLQPGDIGIYRCSARNPASSRTGNEAEVRILSDPGLHRQLYFLQRPSNVVAIEGKDAVLECCVSGYPPPSFTWLRGEEVIQLRSKKYSLLGGSNLLISNVTDDDSGMYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEFECTVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQTSAQLIVPKPAIPSSSVLPSAPRDVVPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVGNLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLQAVSTSPTSILITWEPPAYANGPVQGYRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYSLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNVSLEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSVPDLSTPMLPPVGVQAVALTHDAVRVSWADNSVPKNQKTSEVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGLKPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAYILFYTLDKNIPIDDWIMETISGDRLTHQIMDLNLDTMYYFRIQARNSKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSNLLVIIVVTVGVITVLVVVIVAVICTRRSSAQQRKKRATHSAGKRKGSQKDLRPPDLWIHHEEMEMKNIEKPSGTDPAGRDSPIQSCQDLTPVSHSQSETQLGSKSTSHSGQDTEEAGSSMSTLERSLAARRAPRAKLMIPMDAQSNNPAVVSAIPVPTLESAQYPGILPSPTCGYPHPQFTLRPVPFPTLSVDRGFGAGRSQSVSEGPTTQQPPMLPPSQPEHSSSEEAPSRTIPTACVRPTHPLRSFANPLLPPPMSAIEPKVPYTPLLSQPGPTLPKTHVKTASLGLAGKARSPLLPVSVPTAPEVSEESHKPTEDSANVYEQDDLSEQMASLEGLMKQLNAITGSAF q91562_xenla dcc.L XDCCa dcc protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MENCLGFYSLPKLVFLLYGFTLLNLNLTVSGSQVKSFTSLWFLSEPSDAVTMRGGNVVLNCSAQSDRGAPIIKWKKDGVYLNLVIDERRQQLPSGSFLIQNVVHSRHHRPDEGVYQCEASLDSVGTIVSRTAKVLVAGPLRILSQTESVTAFVGDTALLRCEITGEPMPTISWQKNEEDLKVTPGDPRLLVLPSGTLQISRLQTADGGVYRCLAKNPGSARVGNEAELRILSESGLHRQQVFLQRPSNVVAIEGQDAVLECAVSGYPTPTIVWMQGDEPVPIRTRKYSVLGGSNLLISNVTDDDAGAYTCVATYKNENTSFSADLTVMVPPQFLNHPANLYAYESMDIEFECAVSGKPSPTVKWTKNGEVVIPSDYFQIVDGSNLRILGLVKSDEGYYQCIAENEAGNIQTYAQLIIPDPAVPSSSILPSAPRDVVPVLVSSRFVRLSWRPPVESKGNIQTYTVYFSKQGVQRERAVNTSQPISLQITVGNLTPEETYNFRVVAYNEWGPGESSQEVKVVTQPELQVPGPVENLQVVSTAPTSVLISWDPPAYANGPVQGYRLFCAETFSGREQNIEVDGIVYRLEGLRKFTEYSIRVLAYNRYGPGVSSEEHTVVTLSDVPSAMPQNVSLEVANSRSIKVSWLPPPPGTQNGFITGYKIRHRKTTRRGELETLEPNNLWYLFTGLEKGSQYSFQVAAMTVNGTGPSSDWYTAETPENDLDESQVPDQPSSLHVRPLTTSIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETVRVDSKQRYYSIENLEPSSHYVISLKAFNNAGEGVPLYESATTRSQTVPDMSTPMLPPVGVQAVALTHDAVRVSWADNSVLKNQKTTEVRFYTIRWRTSYSASSKYKSADTTSLSHTVTGLKPNTMYEFSVMVTKGRRSSTWSMTAHATTYETAPTSAPKDLTVITRERKPRAVIVSWQPPIEANGKIIDFILFYTLDKNLQLDDWIMVTITGDRLTHEILDLNLDTAYYFRIQARNAKGLGPLSEPITFRTPKVEHPDKMANDQGRHGDGGYWSVDTNLIDRSSLNEPPIGQMHPPHGSVTPQKNSNLLVIIVVTVGAITILVVVIVAVICTRRSSAQQRKKRATHSAGKRKGSQKDLRPPDLWIHHEEMEMKNIEKPSGSDTQGRDSPRQSCQDITPVSHSQSESQLGSKSTSHSGPDADEVGSNISTLERTLAARRATRAKLMIPMDSQPSSNPPVVSAIPVPTLESAQYPGILPSPTCGYPHPQFTLRPVPFPTLTVDRGFGTSRVTEVPASQQSSVLSHPQPEHSTSEDAPSRTIPTACVRPTHPLRSFANPLLPPPMTAMEPKVPYTTLLSQTGSGLSKAQVKTASLGLAGKARSPLLPVSVPTAPEVSEESHKHTDDPSSVYEQDDLSEQMASLEGLMKQLNAITGSAF maz_mouse Myc-associated zinc finger protein Maz Purine-binding transcription factor Pur-1 protein protein mouse Mus musculus Mouse MFPVFPCTLLAPPFPVLGLDSRGVGGLMNSFPPPQGHAQNPLQVGAELQSRFFASQGCAQSPFQAAPAPPPTPQAPAAEPLQVDLLPVLAAAQESAAAAAAAAAAAAAVVTAPPAPAAASTVDTAALKQPPAPPPPPPAVSAPAAEAAPPAAAATIAAAAATAVVAPTSTVAVAPVASVLEKKTKSKGPYICALCAKEFKNGYNLRRHEAIHTGAKAGRVPSGAMKMPTMVPLSLLSVPQLSGASGGGGEAGAGGGTTAVAAGGVVTTTASGKRIRKNHACEMCGKAFRDVYHLNRHKLSHSDEKPYQCPVCQQRFKRKDRMSYHVRSHDGAVHKPYNCSHCGKSFSRPDHLNSHVRQVHSTERPFKCEKCEAAFATKDRLRAHTVRHEEKVPCHVCGKMLSSAYISDHMKVHSQGPHHVCELCNKGTGEVCPMAAAAAAAAAAAAAVVAAPPTAVGSLSGAEGVPVSSQPLPSQPW dcc_mouse Netrin receptor DCC Dcc Tumor suppressor protein DCC protein protein mouse Mus musculus Mouse MENSLGCVWVPKLAFVLFGASLLSAHLQVTGFQIKPFTSLHFVSEPSDAVTMRGGNVLLNCSAESDRGVPVIKWKKDGLILALGMDDRKQQLPNGSLLIQNILHSRHHKPDEGLYQCEASLADSGSIISRTAKVTVAGPLRFLSQTESITAFMGDTVLLKCEVIGEPMPTIHWQKNQQDLNPLPGDSRVVVLPSGALQISRLQPGDSGVYRCSARNPASIRTGNEAEVRILSDPGLHRQLYFLQRPSNVIAIEGKDAVLECCVSGYPPPSFTWLRGEEVIQLRSKKYSLLGGSNLLISNVTDDDSGTYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEFECAVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQSSAQLIVPKPAIPSSSILPSAPRDVLPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVGNLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLHAVSTSPTSILITWEPPAYANGPVQGYRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYTLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNISLEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSGPDVSTPMLPPVGVQAVALTHEAVRVSWADNSVPKNQKTSDVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGLKPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAYILFYTLDKNIPIDDWIMETISGDRLTHQIMDLSLDTMYYFRIQARNVKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSNLLVITVVTVGVLTVLVVVIVAVICTRRSSAQQRKKRATHSVSKRKGSQKDLRPPDLWIHHEEMEMKNIEKPTGTDPAGRDSPIQSCQDLTPVSHSQSETQMGSKSASHSGQDTEDAGSSMSTLERSLAARRATRAKLMIPMEAQSSNPAVVSAIPVPTLESAQYPGILPSPTCGYPHPQFTLRPVPFPTLSVDRGFGAGRTQSVSEGPTTQQQPMLPPAQPEHPSSEEAPSRTIPTACVRPTHPLRSFANPLLPPPMSAIEPKVPYTPLLSQPGPTLPKTHVKTASLGLAGKARSPLLPVSVPTAPEVSEESHKPTEDPASVYEQDDLSEQMASLEGLMKQLNAITGSAF dcc-naz 764919 N/A 764925 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 764926 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 4B dcc-naz-1 764919 N/A-x 764925 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A-x 764926 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 4B dcc-naz-2 764919 N/A 764927 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 764925 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 4B The cytoplasmic range of DCC used as bait construct was inferred from the human protein. dcc-naz-3 764919 N/A-x-x 764925 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A-x 764927 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 4B The cytoplasmic range of DCC used as bait construct was inferred from the human protein. maz-dcc 764920 N/A 764928 unspecified role unspecified role bait bait N/A 764929 unspecified role unspecified role prey prey physical association physical association false false false 2A The interaction was detected in differentiated P19 EC cells after 3-day or 9-day treatment of cells with RA. DCC is not expressed in undifferentiated P19 EC cells. dcc-maz 764920 N/A-x 764928 unspecified role unspecified role prey prey N/A-x 764929 unspecified role unspecified role bait bait physical association physical association false false false 2B The interaction was detected in differentiated P19 EC cells after 3-day or 9-day treatment of cells with RA. DCC is not expressed in undifferentiated P19 EC cells. maz-dcc-2 764921 N/A 764925 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 764926 unspecified role unspecified role prey prey region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5C MAZ minimal bait construct aa 231-288 interact with DCC prey construct aa 1155-1147 but not with DCC construct aa 1425-1447. dcc-maz-1 764921 N/A-x-x 764925 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A-x 764926 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5B maz-dcc-1 764922 N/A 764925 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 764929 unspecified role unspecified role prey prey physical association physical association false false false no figure maz-dcc-3 764923 N/A 764925 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false mouse-p19 Mus musculus embryonic teratocarcinoma cell line, epithelial-like, induced by retinoic acid p19 mouse embryonic teratocarcinoma cellline N/A 764929 unspecified role unspecified role prey prey physical association physical association false false false 4A The interaction was detected after 3-days treatment with RA. maz-dcc-4 764924 N/A 764928 unspecified role unspecified role neutral component neutral component N/A 764929 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6E