MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint perlman-2001-1 TGF-beta-induced apoptosis is mediated by the adapter protein Daxx that facilitates JNK activation. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Perlman R., Schiemann WP., Brooks MW., Lodish HF., Weinberg RA. Apoptosis - Interactions involving proteins with a function related to apoptosis Strength of interaction determined as units of beta galactosidase activity. Nat. Cell Biol. (1465-7392) 2001 perlman-2001-2 TGF-beta-induced apoptosis is mediated by the adapter protein Daxx that facilitates JNK activation. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 pull down pull down western blot western blot Immuno blot Immuno blot Perlman R., Schiemann WP., Brooks MW., Lodish HF., Weinberg RA. Apoptosis - Interactions involving proteins with a function related to apoptosis Strength of interaction determined as units of beta galactosidase activity. Nat. Cell Biol. (1465-7392) 2001 perlman-2001-3 TGF-beta-induced apoptosis is mediated by the adapter protein Daxx that facilitates JNK activation. human-aml_193 Homo sapiens acute myeloid leukemia cell line aml_193 Acute myeloid leukemia cells coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Perlman R., Schiemann WP., Brooks MW., Lodish HF., Weinberg RA. Apoptosis - Interactions involving proteins with a function related to apoptosis Strength of interaction determined as units of beta galactosidase activity. Nat. Cell Biol. (1465-7392) 2001 tgfr2_human TGF-beta receptor type-2 TGFBR2 Transforming growth factor-beta receptor type II TGF-beta type II receptor protein protein human Homo sapiens Human MGRGLLRGLWPLHIVLWTRIASTIPPHVQKSVNNDMIVTDNNGAVKFPQLCKFCDVRFSTCDNQKSCMSNCSITSICEKPQEVCVAVWRKNDENITLETVCHDPKLPYHDFILEDAASPKCIMKEKKKPGETFFMCSCSSDECNDNIIFSEEYNTSNPDLLLVIFQVTGISLLPPLGVAISVIIIFYCYRVNRQQKLSSTWETGKTRKLMEFSEHCAIILEDDRSDISSTCANNINHNTELLPIELDTLVGKGRFAEVYKAKLKQNTSEQFETVAVKIFPYEEYASWKTEKDIFSDINLKHENILQFLTAEERKTELGKQYWLITAFHAKGNLQEYLTRHVISWEDLRKLGSSLARGIAHLHSDHTPCGRPKMPIVHRDLKSSNILVKNDLTCCLCDFGLSLRLDPTLSVDDLANSGQVGTARYMAPEVLESRMNLENVESFKQTDVYSMALVLWEMTSRCNAVGEVKDYEPPFGSKVREHPCVESMKDNVLRDRGRPEIPSFWLNHQGIQMVCETLTECWDHDPEARLTAQCVAERFSELEHLDRLSGRSCSEEKIPEDGSLNTTK daxx_human Death domain-associated protein 6 DAXX BING2 DAP6 Daxx Fas death domain-associated protein ETS1-associated protein 1 protein protein human Homo sapiens Human MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVDPALSDPVLARRLRENRSLAMSRLDEVISKYAMLQDKSEEGERKKRRARLQGTSSHSADTPEASLDSGEGPSGMASQGCPSASRAETDDEDDEESDEEEEEEEEEEEEEATDSEEEEDLEQMQEGQEDDEEEDEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQLFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSGPPCKKSRKEKKQTGSGPLGNSYVERQRSVHEKNGKKICTLPSPPSPLASLAPVADSSTRVDSPSHGLVTSSLCIPSPARLSQTPHSQPPRPGTCKTSVATQCDPEEIIVLSDSD daf4_caeel Cell surface receptor daf-4 daf-4 C05D2.1 Abnormal dauer formation protein 4 protein protein caeel Caenorhabditis elegans MNQKGTVRLKALVLICLPLFLIATPVPVAVTEDDRQDRIESEAAEKEWANTLVSKVAQSNGTGTIKVSAPAKPTLRRMDNEEDEVISIECVYYDEMECEKSGDCEITKKTCYSEAHLKAVGCLAVFGLPTQEINSTEPYLKVDKPQYKSLGCMPYQHADSMNCENESSCRQGRSFRGGIGMCCCSTNNCNMPDLIEMVNPSLKKDSDNSALLWASTPSNMDLESLDKFPFYWIIIIALSVILCIALLILAYVGWKFQQNKKEEIKKQQKIKFDMEKTDALEAGNVPLVEPEEEMIEMVETPKELPITDFQLISKGRFGKVFKAQYTPDSGEKRLVAVKKLNEFQKASFLAEKRIFDELNEYPKWYKSIVEFVCAEKIGDEYWIVTEFHERLSLYELLKNNVISITSANRIIMSMIDGLQFLHDDRPYFFGHPKKPIIHRDIKSKNILVKSDMTTCIADFGLARIYSYDIEQSDLLGQVGTKRYMSPEMLEGATEFTPTAFKAMDVYSMGLVMWEVISRTKLHQTDEPPNYQMPFQVIGFDPTIGLMRNYVVSKKERPQWRDEIIKHEYMSLLKKVTEEMWDPEACARITAGCAFARVWNHIMSSPDSSEGYHSGSSMKNRGVDDVEQSEKPEGIEEMQHYHASSPSKRQHPSPNPFFDSCPPPPPIPVILENGGILQPDNAEPEPEELPDLPIVEKIYDIATNMLFSREELDLMNAQRQVEYEAGADTRASTPTPSGTFGTFTT avr2a_human Activin receptor type-2A ACVR2A ACVR2 Activin receptor type IIA protein protein human Homo sapiens Human MGAAAKLAFAVFLISCSSGAILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTDCVEKKDSPEVYFCCCEGNMCNEKFSYFPEMEVTQPTSNPVTPKPPYYNILLYSLVPLMLIAGIVICAFWVYRHHKMAYPPVLVPTQDPGPPPPSPLLGLKPLQLLEVKARGRFGCVWKAQLLNEYVAVKIFPIQDKQSWQNEYEVYSLPGMKHENILQFIGAEKRGTSVDVDLWLITAFHEKGSLSDFLKANVVSWNELCHIAETMARGLAYLHEDIPGLKDGHKPAISHRDIKSKNVLLKNNLTACIADFGLALKFEAGKSAGDTHGQVGTRRYMAPEVLEGAINFQRDAFLRIDMYAMGLVLWELASRCTAADGPVDEYMLPFEEEIGQHPSLEDMQEVVVHKKKRPVLRDYWQKHAGMAMLCETIEECWDHDAEARLSAGCVGERITQMQRLTNIITTEDIVTVVTMVTNVDFPPKESSL tnr6_mouse Tumor necrosis factor receptor superfamily member 6 Fas Apt1 Tnfrsf6 FASLG receptor Apoptosis-mediating surface antigen FAS Apo-1 antigen protein protein mouse Mus musculus Mouse MLWIWAVLPLVLAGSQLRVHTQGTNSISESLKLRRRVRETDKNCSEGLYQGGPFCCQPCQPGKKKVEDCKMNGGTPTCAPCTEGKEYMDKNHYADKCRRCTLCDEEHGLEVETNCTLTQNTKCKCKPDFYCDSPGCEHCVRCASCEHGTLEPCTATSNTNCRKQSPRNRLWLLTILVLLIPLVFIYRKYRKRKCWKRRQDDPESRTSSRETIPMNASNLSLSKYIPRIAEDMTIQEAKKFARENNIKEGKIDEIMHDSIQDTAEQKVQLLLCWYQSHGKSDAYQDLIKGLKKAECRRTLDKFQDMVQKDLGKSTPDTGNENEGQCLE daxx_mouse Death domain-associated protein 6 Daxx Daxx protein protein mouse Mus musculus Mouse MATDDSIIVLDDDDEDEAAAQPGPSNLPPNPASTGPGPGLSQQATGLSEPRVDGGSSNSGSRKCYKLDNEKLFEEFLELCKTETSDHPEVVPFLHKLQQRAQSVFLASAEFCNILSRVLARSRKRPAKIYVYINELCTVLKAHSIKKKLNLAPAASTTSEASGPNPPTEPPSDLTNTENTASEASRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSSYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGLDTFPDYGDVLRAVEKAATRHSLGLPRQQLQLLAQDAFRDVGVRLQERRHLDLIYNFGCHLTDDYRPGVDPALSDPTLARRLRENRTLAMNRLDEVISKYAMMQDKTEEGERQKRRARLLGTAPQPSDPPQASSESGEGPSGMASQECPTTSKAETDDDDDDDDDDDEDNEESEEEEEEEEEEKEATEDEDEDLEQLQEDQGGDEEEEGGDNEGNESPTSPSDFFHRRNSEPAEGLRTPEGQQKRGLTETPASPPGASLDPPSTDAESSGEQLLEPLLGDESPVSQLAELEMEALPEERDISSPRKKSEDSLPTILENGAAVVTSTSVNGRVSSHTWRDASPPSKRFRKEKKQLGSGLLGNSYIKEPMAQQDSGQNTSVQPMPSPPLASVASVADSSTRVDSPSHELVTSSLCSPSPSLLLQTPQAQSLRQCIYKTSVATQCDPEEIIVLSDSD daxx-tgfbr2-1 Interaction detected by Y2H. 7415 N/A 7418 unspecified role unspecified role bait bait N/A 7419 unspecified role unspecified role prey prey physical association physical association false false false AA 489-740 of Daxx were used in the experiment. K277 of TGFbetaR2 is necessary for this interaction. daxx-daf_4-1 Interaction detected by Y2H. 7415 N/A 7419 unspecified role unspecified role prey prey N/A 7420 unspecified role unspecified role bait bait physical association physical association false false false AA 489-740 of Daxx sufficient for interaction. daxx-acvr2-1 Interaction determined by Y2H. 7415 N/A 7421 unspecified role unspecified role bait bait N/A 7419 unspecified role unspecified role prey prey physical association physical association false false false AA 489-740 sufficient for interaction. daxx-tnfrsf6-1 interaction detected by Y2H. 7415 N/A 7419 unspecified role unspecified role prey prey N/A 7422 unspecified role unspecified role bait bait physical association physical association false false false AA 489-740 of Daxx sufficient for interaction. Death Domain of Fas used in the experiemnt (IPR000483). daxx-daxx-1 Interaction determined by Y2H. 7415 N/A 7419 unspecified role unspecified role bait bait N/A 7419 unspecified role unspecified role prey prey physical association physical association false false false AA 489-740 sufficient fo rinteraction. daxx-tnfrsf6-2 Interaction detected by GST-pull down. 7416 N/A 7423 unspecified role unspecified role prey prey N/A 7422 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Cytoplasmic domian of Fas used in the experiment. Fas is GST-tagged while murine Daxx is HA tagged. daxx-tgfbr2-2 interaction detected by GST pull down. 7416 N/A 7423 unspecified role unspecified role prey prey N/A 7418 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Cytoplasmic domian of TGFBr2 was used in the experiment. TGFBR2 was GST tagged while murine Daxx was HA tagged. tgfbr2-daxx-1 Interaction detected by CoIP. 7417 N/A 7419 unspecified role unspecified role bait bait N/A 7418 unspecified role unspecified role prey prey physical association physical association false false false HA tagged TGFBR2.